##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935002.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	876288
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.106580256719255	33.0	31.0	34.0	30.0	34.0
2	32.28678813358165	34.0	31.0	34.0	30.0	34.0
3	32.297721753578735	34.0	31.0	34.0	30.0	34.0
4	35.85907487036226	37.0	35.0	37.0	35.0	37.0
5	35.7712030747882	37.0	35.0	37.0	35.0	37.0
6	35.700361068507156	37.0	35.0	37.0	33.0	37.0
7	36.026872443762784	37.0	35.0	37.0	35.0	37.0
8	35.747495115761026	37.0	35.0	37.0	35.0	37.0
9	37.506066498685364	39.0	37.0	39.0	35.0	39.0
10	37.31335245946538	39.0	37.0	39.0	34.0	39.0
11	37.24611315001461	39.0	37.0	39.0	34.0	39.0
12	37.11025713007596	39.0	37.0	39.0	34.0	39.0
13	36.97717987693544	39.0	37.0	39.0	33.0	39.0
14	38.05981252738825	40.0	37.0	41.0	33.0	41.0
15	38.14437034399649	40.0	37.0	41.0	33.0	41.0
16	38.23592015410458	40.0	37.0	41.0	34.0	41.0
17	38.25515698035349	40.0	37.0	41.0	34.0	41.0
18	38.28303023663453	40.0	37.0	41.0	34.0	41.0
19	38.269163790899796	40.0	37.0	41.0	34.0	41.0
20	38.21027789950336	40.0	37.0	41.0	34.0	41.0
21	38.16833849145486	40.0	37.0	41.0	34.0	41.0
22	38.087843266140816	40.0	37.0	41.0	34.0	41.0
23	37.856839303973125	40.0	36.0	41.0	33.0	41.0
24	37.615287439745835	39.0	36.0	41.0	33.0	41.0
25	37.59355257632194	39.0	36.0	41.0	33.0	41.0
26	37.784371119997076	39.0	36.0	41.0	33.0	41.0
27	37.78575650927549	39.0	36.0	41.0	33.0	41.0
28	37.78203855353491	40.0	36.0	41.0	33.0	41.0
29	37.84327983494011	40.0	36.0	41.0	34.0	41.0
30	37.6688257741747	39.0	36.0	41.0	33.0	41.0
31	37.52780136210926	39.0	36.0	41.0	33.0	41.0
32	37.45394322414548	39.0	35.0	41.0	33.0	41.0
33	37.395002556237216	39.0	35.0	41.0	33.0	41.0
34	37.269922673824134	39.0	35.0	41.0	33.0	41.0
35	37.208768121896	39.0	35.0	41.0	32.0	41.0
36	37.13503094872919	39.0	35.0	41.0	32.0	41.0
37	36.99702495070114	39.0	35.0	41.0	31.0	41.0
38	37.011999479623135	39.0	35.0	41.0	32.0	41.0
39	37.01622297692083	39.0	35.0	41.0	32.0	41.0
40	36.91881778593339	39.0	35.0	41.0	31.0	41.0
41	36.879673121165645	39.0	35.0	41.0	31.0	41.0
42	36.929045017163304	39.0	35.0	41.0	32.0	41.0
43	36.86380733274905	39.0	35.0	41.0	32.0	41.0
44	36.71444319675723	39.0	35.0	41.0	31.0	41.0
45	36.7149202088811	39.0	35.0	41.0	31.0	41.0
46	36.558497891104295	39.0	35.0	40.0	31.0	41.0
47	36.445457429520886	39.0	35.0	40.0	31.0	41.0
48	36.46649389241893	39.0	35.0	40.0	31.0	41.0
49	36.46864044697634	39.0	35.0	40.0	31.0	41.0
50	36.39894304155711	39.0	35.0	40.0	31.0	41.0
51	35.357622151621385	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	7.0
11	8.0
12	3.0
13	10.0
14	10.0
15	18.0
16	41.0
17	73.0
18	194.0
19	336.0
20	649.0
21	1038.0
22	1682.0
23	2459.0
24	3285.0
25	4096.0
26	5039.0
27	5959.0
28	7493.0
29	9317.0
30	11912.0
31	15660.0
32	21159.0
33	29611.0
34	52722.0
35	79545.0
36	67094.0
37	106664.0
38	195180.0
39	254794.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07849017674555	23.07529031551271	25.977646618463336	10.868572889278411
2	30.23629217791411	26.30322451066316	24.95207055214724	18.50841275927549
3	29.92418017820625	25.49093448729185	28.022978746713413	16.56190658778849
4	27.79257504382121	25.237593120070112	28.26627775343266	18.703554082676018
5	25.016090600350573	29.395929192229037	25.528022750511248	20.059957456909146
6	24.81410221297108	32.97477541630149	26.08971023225241	16.121412138475023
7	76.7981531186094	6.298043565585744	10.842782281624306	6.061021034180544
8	75.9513995398773	5.731106668127374	10.019651073619631	8.297842718375694
9	70.35415297253871	7.455882084428864	12.401174043236926	9.7887908997955
10	38.052786298568506	27.455471260590127	19.617865359333916	14.87387708150745
11	26.995919149868534	23.98378158778849	28.40116491381829	20.619134348524685
12	24.382394829097283	21.26093247881975	33.556091148115684	20.80058154396728
13	21.171464176161262	26.12679849547181	35.10706525708443	17.5946720712825
14	17.14253761320479	32.1603171560035	29.715572962313757	20.981572268477944
15	14.881522969617295	26.173016177329828	38.495220749342685	20.450240103710197
16	16.029889716622844	26.452034034472682	33.69086419076833	23.827212058136137
17	16.157359224364594	26.366217499269645	29.06122188139059	28.41520139497517
18	17.870722867367807	26.37340691644756	33.93530437481741	21.82056584136722
19	18.45066918638621	27.648101993865033	29.831744814490214	24.069484005258545
20	20.516998977505114	27.614094909436165	31.47914840782939	20.389757705229332
21	20.01031624306164	29.015917141396436	30.523184158632777	20.450582456909146
22	18.096105390008766	26.648544770669	28.134700007303536	27.120649832018696
23	16.777360867659947	28.586377994449315	28.637502738825592	25.998758399065146
24	19.997649174700555	25.078855353491093	30.93994211948583	23.983553352322524
25	16.53463244960561	29.583766980718668	28.125	25.756600569675726
26	18.994668419515044	28.38758490359334	29.01603125912942	23.601715417762197
27	20.282487036225533	28.427640227870288	29.401977432077125	21.88789530382705
28	18.88157774612913	25.168780127081508	32.13076066316097	23.818881463628397
29	23.398814088518844	21.76807168419515	29.815996567338594	25.017117659947413
30	21.4108831799591	26.67228125912942	29.866778958515923	22.05005660239556
31	25.34600496640374	28.485840271691497	23.68159783085013	22.48655693105463
32	25.51820862547473	28.51071793748174	23.724734333917617	22.246339103125912
33	21.976108311422728	25.70159582237803	24.846739884604148	27.47555598159509
34	22.692311203622552	26.424531660823842	25.967946611159803	24.915210524393807
35	22.168054338299736	26.25358329681566	25.760366454864155	25.817995910020453
36	24.75179393076249	24.124374634823255	26.591600021910605	24.532231412503652
37	21.914712971078	26.57265647823547	30.970069200993283	20.54256134969325
38	21.997562445223487	27.71543145632486	24.07678754016944	26.21021855828221
39	22.708173568507156	25.141620106631606	27.020796815658777	25.129409509202453
40	21.900448254455156	26.370325737657023	27.726614994157174	24.002611013730647
41	18.354239702015775	28.05196465089103	25.786841768916158	27.806953878177037
42	22.185628469179083	23.635380148992112	28.91891706836109	25.26007431346772
43	25.148124817411627	21.244157172071283	28.046372881974875	25.56134512854221
44	22.373123904469765	22.972242002629272	27.701166739702014	26.953467353198953
45	22.784404214139645	23.352025817995912	25.907121859479986	27.956448108384457
46	25.65047107800175	24.109425211802513	27.621512562080046	22.618591148115687
47	18.06084301051709	24.76434688139059	32.35146435874964	24.823345749342682
48	20.164375182588373	22.437258070406077	30.03350496640374	27.364861780601814
49	21.667533961437336	19.136630879345603	32.837149430324274	26.358685728892784
50	21.275311313175578	20.3661353345019	30.897033851884316	27.461519500438214
51	19.41530638328951	20.979175796085308	26.371923385918784	33.2335944347064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	904.0
1	999.0
2	1094.0
3	1456.0
4	1818.0
5	1351.5
6	885.0
7	1006.5
8	1128.0
9	1353.5
10	1579.0
11	1682.0
12	1785.0
13	2016.0
14	2247.0
15	2187.0
16	2127.0
17	2049.0
18	1971.0
19	2257.0
20	2543.0
21	2571.5
22	2600.0
23	3480.5
24	4361.0
25	4991.0
26	6285.5
27	6950.0
28	8217.5
29	9485.0
30	10906.0
31	12327.0
32	14792.5
33	17258.0
34	18795.0
35	20332.0
36	21908.5
37	23485.0
38	26303.0
39	29121.0
40	32009.5
41	34898.0
42	38558.5
43	42219.0
44	49585.0
45	56951.0
46	64151.5
47	71352.0
48	89622.0
49	107892.0
50	115420.0
51	122948.0
52	117489.0
53	112030.0
54	88126.5
55	64223.0
56	51551.0
57	38879.0
58	31067.5
59	23256.0
60	20383.5
61	17511.0
62	14781.5
63	12052.0
64	10090.5
65	8129.0
66	7242.5
67	6356.0
68	4881.5
69	3407.0
70	2501.0
71	1595.0
72	1512.5
73	1430.0
74	1082.5
75	530.5
76	326.0
77	268.0
78	210.0
79	191.5
80	173.0
81	108.0
82	43.0
83	35.5
84	28.0
85	21.0
86	14.0
87	9.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	876288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.15345402042125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.50124597562792	14.06771932960533
2	12.772637478410692	5.659160741949609
3	6.471821624208451	4.301196083408097
4	3.7866189383060247	3.3554675417047535
5	2.467507516458097	2.7331907155449144
6	1.7412379643653102	2.3144661109307276
7	1.2276733373976132	1.903804338249459
8	0.9316376589454458	1.6511193632912664
9	0.7639634326381198	1.5231985900408596
>10	4.633218947501741	20.240221519851403
>50	1.0976239365924407	17.758530069288973
>100	0.5855383506047129	18.179879406254216
>500	0.012502598233552587	1.7918021930025771
>1k	0.005730357523711604	3.012341932355614
>5k	0.0010418831861293823	1.5079020645221997
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	6728	0.7677841075080339	TruSeq Adapter, Index 18 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	6338	0.723278191644756	RNA PCR Primer, Index 34 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	4478	0.5110192082968156	RNA PCR Primer, Index 34 (96% over 26bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	3638	0.41516031259129416	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	3464	0.3953038270522933	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	3290	0.37544734151329245	RNA PCR Primer, Index 34 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2895	0.3303708369851008	TruSeq Adapter, Index 18 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2321	0.26486725825299445	TruSeq Adapter, Index 3 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	1408	0.16067776803973122	TruSeq Adapter, Index 3 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	1279	0.14595658048495472	RNA PCR Primer, Index 34 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	1152	0.13146362839614373	RNA PCR Primer, Index 34 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC	1139	0.1299800978673678	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	1038	0.11845420683610867	TruSeq Adapter, Index 18 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	981	0.11194949605609116	RNA PCR Primer, Index 34 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	908	0.10361890154834939	TruSeq Adapter, Index 3 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.282354659655273E-4	0.0	0.0	0.5387498174116272	0.0
2	2.282354659655273E-4	0.0	0.0	2.7593667835232254	0.0
3	2.282354659655273E-4	0.0	0.0	3.815640520011686	0.0
4	2.282354659655273E-4	0.0	0.0	5.0918191279579315	0.0
5	2.282354659655273E-4	0.0	0.0	8.560085268770084	0.0
6	2.282354659655273E-4	0.0	0.0	10.06335816535203	0.0
7	2.282354659655273E-4	0.0	0.0	11.803197122407244	0.0
8	2.282354659655273E-4	0.0	0.0	14.382257887817703	0.0
9	2.282354659655273E-4	0.0	0.0	15.317110356412504	0.0
10	2.282354659655273E-4	0.0	0.0	17.76676161262051	0.0
11	2.282354659655273E-4	0.0	0.0	20.845772166228453	0.0
12	2.282354659655273E-4	0.0	0.0	23.624995435290682	0.0
13	2.282354659655273E-4	0.0	0.0	24.53086199970786	0.0
14	2.282354659655273E-4	0.0	0.0	24.872872845457202	0.0
15	2.282354659655273E-4	0.0	0.0	25.51980627373649	0.0
16	2.282354659655273E-4	0.0	0.0	26.824057843996496	0.0
17	2.282354659655273E-4	0.0	0.0	28.49462733713117	0.0
18	2.282354659655273E-4	0.0	0.0	30.335232252410165	0.0
19	2.282354659655273E-4	0.0	0.0	31.50402607361963	0.0
20	2.282354659655273E-4	0.0	0.0	32.44914913818288	0.0
21	2.282354659655273E-4	0.0	0.0	33.57628998685364	0.0
22	2.282354659655273E-4	0.0	0.0	34.727509677183754	0.0
23	3.42353198948291E-4	0.0	0.0	35.89402114373357	0.0
24	3.42353198948291E-4	0.0	0.0	36.82065713555361	0.0
25	5.705886649138183E-4	0.0	0.0	37.5900388913234	0.0
26	5.705886649138183E-4	0.0	0.0	38.27223469909436	0.0
27	5.705886649138183E-4	0.0	0.0	38.934231668127374	0.0
28	5.705886649138183E-4	0.0	0.0	39.62487218813906	0.0
29	5.705886649138183E-4	0.0	0.0	40.31882212240725	0.0
30	5.705886649138183E-4	0.0	0.0	41.169455704060766	0.0
31	5.705886649138183E-4	0.0	0.0	41.88702800905639	0.0
32	5.705886649138183E-4	0.0	0.0	42.5575838080631	0.0
33	5.705886649138183E-4	0.0	0.0	43.20383252994449	0.0
34	6.84706397896582E-4	0.0	0.0	43.808085926088225	0.0
35	6.84706397896582E-4	0.0	0.0	44.51926763803681	0.0
36	6.84706397896582E-4	0.0	0.0	45.12637397750511	0.0
37	6.84706397896582E-4	0.0	0.0	45.74466385480573	0.0
38	7.988241308793456E-4	0.0	0.0	46.35119960560912	0.0
39	7.988241308793456E-4	0.0	0.0	46.90170354951797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	75	0.0	45.000004	22
CTACTAT	30	2.1649084E-6	45.000004	33
CGTATAA	30	2.1649084E-6	45.000004	26
GCGAAGT	30	2.1649084E-6	45.000004	12
TTATCCG	30	2.1649084E-6	45.000004	12
ATCGTCC	30	2.1649084E-6	45.000004	31
GTTACTT	30	2.1649084E-6	45.000004	32
ACTAGCG	30	2.1649084E-6	45.000004	1
TGCGCGT	30	2.1649084E-6	45.000004	37
ACGTCGG	60	0.0	45.000004	2
CATCGGT	30	2.1649084E-6	45.000004	18
ATCGGTT	30	2.1649084E-6	45.000004	19
GCGCTTA	30	2.1649084E-6	45.000004	14
TACGTCG	30	2.1649084E-6	45.000004	1
CGAAGTA	30	2.1649084E-6	45.000004	13
TATCCGA	30	2.1649084E-6	45.000004	13
TCACGCG	20	7.032368E-4	45.0	40
CTATGCG	20	7.032368E-4	45.0	1
TTAGGCA	25	3.890044E-5	45.0	15
TCGCATA	20	7.032368E-4	45.0	1
>>END_MODULE