FastQCFastQC Report
Sat 14 Jan 2017
SRR2935001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406909
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC120090.8535733299026448TruSeq Adapter, Index 14 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC99800.7093564686841863TruSeq Adapter, Index 14 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC98280.6985526427082348No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC92410.6568299726563694TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG89930.6392026776429748No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT68690.48823342518954677Illumina PCR Primer Index 3 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC45060.3202765779449843TruSeq Adapter, Index 14 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC34450.24486302952074368Illumina PCR Primer Index 3 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG26370.1874321651222645Illumina PCR Primer Index 3 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT23220.1650426573431544No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTGCGTCT20980.14912122958912055No Hit
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG20170.14336392758877795TruSeq Adapter, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC19760.14044973768736999No Hit
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG19540.13888602603295594Illumina PCR Primer Index 3 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG19230.1366826141562816TruSeq Adapter, Index 14 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG17970.12772681104463757Illumina PCR Primer Index 3 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC17850.12687387741495718No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC16180.11500388440190516No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTA15970.1135112505499645No Hit
GGCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT15200.10803825975951536Illumina PCR Primer Index 3 (95% over 21bp)
AGCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT14640.10405790282100691TruSeq Adapter, Index 14 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT14450.10270742457401297Illumina PCR Primer Index 3 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG650.045.0000041
GTATACG750.045.0000041
CTATGCG453.8562575E-1045.01
AGCGTAA453.8562575E-1045.025
ATCTCGC351.2121927E-745.037
TCTTGCG551.8189894E-1245.01
CGAACGA207.0338266E-445.012
TCCGCGC207.0338266E-445.014
CGTATAT207.0338266E-445.041
GGTCGTC207.0338266E-445.09
CCGGCCA207.0338266E-445.022
GCAACGT207.0338266E-445.036
CGTTGTC207.0338266E-445.020
ATTACTC351.2121927E-745.010
GAATCCG207.0338266E-445.024
GCACGGT351.2121927E-745.03
TATGCGT207.0338266E-445.036
CATTCGA453.8562575E-1045.012
ATCCGCG207.0338266E-445.01
ATATCGG207.0338266E-445.033
Produced by FastQC (version 0.11.5)