##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935001.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1406909
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21665082816302	33.0	31.0	34.0	30.0	34.0
2	32.40285761197064	34.0	31.0	34.0	30.0	34.0
3	32.40393586223416	34.0	31.0	34.0	30.0	34.0
4	35.94830013881495	37.0	35.0	37.0	35.0	37.0
5	35.86313542666939	37.0	35.0	37.0	35.0	37.0
6	35.7921749025701	37.0	35.0	37.0	35.0	37.0
7	36.097512347991234	37.0	35.0	37.0	35.0	37.0
8	35.79440390245567	37.0	35.0	37.0	35.0	37.0
9	37.58127640096126	39.0	38.0	39.0	35.0	39.0
10	37.39853039535606	39.0	37.0	39.0	34.0	39.0
11	37.33987414964294	39.0	37.0	39.0	34.0	39.0
12	37.293343066253755	39.0	37.0	39.0	34.0	39.0
13	37.22327030390736	39.0	37.0	39.0	34.0	39.0
14	38.35089334136039	40.0	38.0	41.0	34.0	41.0
15	38.36728601494482	40.0	38.0	41.0	34.0	41.0
16	38.4468014633498	40.0	38.0	41.0	34.0	41.0
17	38.440422941355834	40.0	38.0	41.0	34.0	41.0
18	38.449073109916846	40.0	38.0	41.0	34.0	41.0
19	38.44009314035236	40.0	37.0	41.0	34.0	41.0
20	38.38085689977106	40.0	37.0	41.0	34.0	41.0
21	38.37822062407732	40.0	37.0	41.0	34.0	41.0
22	38.29646124944826	40.0	37.0	41.0	34.0	41.0
23	38.04807418248089	40.0	37.0	41.0	34.0	41.0
24	37.85064776755284	40.0	36.0	41.0	33.0	41.0
25	37.8187281480181	40.0	36.0	41.0	33.0	41.0
26	37.975944428530916	40.0	37.0	41.0	34.0	41.0
27	38.03710474522517	40.0	37.0	41.0	34.0	41.0
28	38.05822551423013	40.0	37.0	41.0	34.0	41.0
29	38.09154323413952	40.0	37.0	41.0	34.0	41.0
30	38.04370218685075	40.0	37.0	41.0	34.0	41.0
31	37.95236152444828	40.0	37.0	41.0	34.0	41.0
32	37.81978791805298	40.0	37.0	41.0	34.0	41.0
33	37.79248622334494	40.0	37.0	41.0	34.0	41.0
34	37.69874526355294	40.0	37.0	41.0	33.0	41.0
35	37.614416426364464	40.0	36.0	41.0	33.0	41.0
36	37.56060555444595	40.0	36.0	41.0	33.0	41.0
37	37.49556723284875	40.0	36.0	41.0	33.0	41.0
38	37.412360003383306	40.0	36.0	41.0	33.0	41.0
39	37.33455397612781	40.0	36.0	41.0	33.0	41.0
40	37.21257025152302	39.0	36.0	41.0	32.0	41.0
41	37.10154956717172	39.0	35.0	41.0	32.0	41.0
42	37.10684628501204	39.0	35.0	41.0	32.0	41.0
43	37.01550206871944	39.0	35.0	41.0	32.0	41.0
44	36.884890209672406	39.0	35.0	41.0	31.0	41.0
45	36.87742064341048	39.0	35.0	41.0	31.0	41.0
46	36.662371198137194	39.0	35.0	41.0	31.0	41.0
47	36.51778757545797	39.0	35.0	41.0	31.0	41.0
48	36.4983676982662	39.0	35.0	41.0	31.0	41.0
49	36.47523613822927	39.0	35.0	40.0	31.0	41.0
50	36.38351734191764	39.0	35.0	40.0	31.0	41.0
51	35.07225911555047	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	16.0
10	20.0
11	19.0
12	14.0
13	15.0
14	23.0
15	21.0
16	47.0
17	110.0
18	234.0
19	486.0
20	912.0
21	1659.0
22	2681.0
23	3739.0
24	5262.0
25	6505.0
26	7661.0
27	8956.0
28	10930.0
29	13276.0
30	16951.0
31	22951.0
32	30500.0
33	43226.0
34	76314.0
35	109739.0
36	102823.0
37	166860.0
38	319459.0
39	455149.0
40	346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.75659335465193	22.772688212244006	27.236942829991133	11.233775603112923
2	30.40075797368558	28.209002856616884	23.970349183920213	17.41988998577733
3	29.141117158252595	27.005229193927967	26.622190916399003	17.23146273142044
4	26.991724411458023	25.587298112386797	28.84450948853124	18.576467987623932
5	26.57378693291464	28.266646954422782	24.503290546865504	20.656275565797078
6	23.959047813326944	33.70751057815396	25.630371260685656	16.703070347833442
7	76.49123006534182	5.293661494808833	12.224529091789163	5.99057934806018
8	74.76290222039947	6.160739607181417	10.727275182687722	8.34908298973139
9	69.10041800855635	7.024974607455066	12.875175295630351	10.999432088358239
10	35.583040552018645	31.06967117276242	19.356760103176537	13.9905281720424
11	24.21379065739149	23.814688796503543	30.579163257893722	21.39235728821125
12	22.830972010272163	20.863822749019302	35.377767858475565	20.927437382232966
13	21.763809883937057	25.430713713537976	35.45247062887507	17.353005773649897
14	17.346466615822344	30.731980533211456	30.552295848558792	21.369257002407405
15	14.401500025943397	27.165296405097983	38.35898412761593	20.074219441342688
16	16.57299796930718	29.05255421637078	30.8626215341575	23.51182628016453
17	17.307089513252098	27.564469343788407	29.658990027073536	25.46945111588596
18	17.851900869210446	26.993785667729753	32.95614712820801	22.198166334851795
19	17.39366227666466	28.497791968066167	30.10450569297659	24.004040062292585
20	19.539856522347925	29.430901358936506	31.1303005382722	19.89894158044337
21	19.652372683663263	29.84500063614633	31.10286450651748	19.399762173672926
22	17.521104776499406	26.349607543913645	29.843650157899337	26.285637521687615
23	16.926823270019597	28.306024056993024	30.734681489705444	24.03247118328193
24	20.38063584780537	24.956198304225786	29.220368908010396	25.442796939958445
25	16.17354071940687	30.68556672819635	27.561128687072156	25.579763865324622
26	16.093364958216913	30.75984303178102	29.34077470540028	23.806017304601788
27	18.420594366799843	29.696376951174525	30.386968880005742	21.49605980201989
28	14.863718975427693	27.340432110392353	33.85087450574273	23.94497440843722
29	19.559900462645416	25.922785340061083	30.192784323648507	24.32452987364499
30	19.790832242881383	26.360908914506908	31.495000742763036	22.353258099848674
31	21.721731824872826	27.89100076835104	27.763558268516302	22.62370913825983
32	22.345368463774133	28.55003415288409	27.222016491471727	21.88258089187005
33	20.003781339091585	23.93608968312805	28.044315588286096	28.015813389494276
34	21.90667626690852	23.945045486239692	29.973864692030546	24.174413554821243
35	19.059299499825507	24.101203418273677	31.014656953648036	25.824840128252784
36	19.704330557271295	24.280461636111504	31.13221963893898	24.88298816767822
37	20.787343033557963	22.66187791818803	35.64068464982454	20.910094398429464
38	19.831701979303563	24.260844162628857	30.046435128355846	25.86101872971173
39	24.79122672468511	22.166110245936306	28.0624404279168	24.980222601461787
40	22.692938917868887	21.700621717538233	31.632891679561364	23.973547685031512
41	20.150059456581772	24.176972355710284	28.733201649857953	26.939766537849998
42	19.845988617600714	22.41985800076622	33.17051778046768	24.563635601165394
43	22.457387080472156	21.6988447724764	30.23273004863854	25.6110380984129
44	20.418804627733564	22.007038124000914	30.37573858721495	27.19841866105057
45	22.087569274203236	20.408427268572453	28.933285663820474	28.570717793403837
46	25.05719986154044	22.003484233877245	29.905345690446218	23.033970214136097
47	17.90670185491741	21.633097805188537	36.162040330966676	24.298160008927372
48	18.964410633523563	20.327327495950342	32.632743126954196	28.075518743571905
49	20.460456219982955	18.08318803845878	36.18983175173376	25.266523989824503
50	20.68861596592246	18.371195294080852	33.010166258087764	27.93002248190892
51	19.286464156530382	18.598075639575836	29.010120768294183	33.1053394355996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1648.0
1	1723.0
2	1798.0
3	2437.0
4	3076.0
5	2382.0
6	1688.0
7	1828.5
8	1969.0
9	2284.5
10	2600.0
11	2740.0
12	2880.0
13	3051.0
14	3222.0
15	3306.5
16	3391.0
17	3550.5
18	3710.0
19	3796.0
20	3882.0
21	4493.0
22	5104.0
23	5759.5
24	6415.0
25	7075.0
26	9903.5
27	12072.0
28	15194.5
29	18317.0
30	21679.5
31	25042.0
32	26299.5
33	27557.0
34	30698.5
35	33840.0
36	36318.5
37	38797.0
38	42937.5
39	47078.0
40	55568.5
41	64059.0
42	74301.0
43	84543.0
44	98494.0
45	112445.0
46	152447.5
47	192450.0
48	199382.5
49	206315.0
50	196350.0
51	186385.0
52	146148.0
53	105911.0
54	85043.0
55	64175.0
56	53631.0
57	43087.0
58	35996.0
59	28905.0
60	25559.5
61	22214.0
62	19963.5
63	17713.0
64	14209.5
65	10706.0
66	8734.5
67	6763.0
68	5195.0
69	3627.0
70	2974.0
71	2321.0
72	2079.0
73	1837.0
74	1269.5
75	631.5
76	561.0
77	343.0
78	125.0
79	103.0
80	81.0
81	106.5
82	132.0
83	76.0
84	20.0
85	12.0
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1406909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.274158038587593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.68086969336877	12.505277001208862
2	12.93007963520583	5.24292955951372
3	6.593744091393106	4.010478293247209
4	4.012953295437183	3.254369972526573
5	2.6814149777279788	2.7181715512746436
6	1.8445554310140018	2.2438084991587752
7	1.3816410468189588	1.9608126255065017
8	1.0683844920872125	1.7328476830841821
9	0.8125270478169524	1.4825971600261116
>10	5.146711453417272	20.677176516025987
>50	1.2331823804990358	18.163520875131187
>100	0.5877512692280867	16.679039922934457
>500	0.015923423910179254	2.2168652959351522
>1k	0.00813863888742495	3.028613486074832
>5k	0.0017692693233532502	3.2219551893383263
>10k+	3.5385386467065E-4	0.8615363690134702
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	12009	0.8535733299026448	TruSeq Adapter, Index 14 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	9980	0.7093564686841863	TruSeq Adapter, Index 14 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC	9828	0.6985526427082348	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	9241	0.6568299726563694	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG	8993	0.6392026776429748	No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	6869	0.48823342518954677	Illumina PCR Primer Index 3 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	4506	0.3202765779449843	TruSeq Adapter, Index 14 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3445	0.24486302952074368	Illumina PCR Primer Index 3 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2637	0.1874321651222645	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT	2322	0.1650426573431544	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTGCGTCT	2098	0.14912122958912055	No Hit
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2017	0.14336392758877795	TruSeq Adapter, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	1976	0.14044973768736999	No Hit
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1954	0.13888602603295594	Illumina PCR Primer Index 3 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1923	0.1366826141562816	TruSeq Adapter, Index 14 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1797	0.12772681104463757	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	1785	0.12687387741495718	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC	1618	0.11500388440190516	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTA	1597	0.1135112505499645	No Hit
GGCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1520	0.10803825975951536	Illumina PCR Primer Index 3 (95% over 21bp)
AGCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1464	0.10405790282100691	TruSeq Adapter, Index 14 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1445	0.10270742457401297	Illumina PCR Primer Index 3 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.107780247336537E-5	0.0	0.0	0.5228483149940757	0.0
2	7.107780247336537E-5	0.0	0.0	2.7740244749305036	0.0
3	7.107780247336537E-5	0.0	0.0	3.911553625714243	0.0
4	7.107780247336537E-5	0.0	0.0	5.367369175973713	0.0
5	7.107780247336537E-5	0.0	0.0	9.289868783268854	0.0
6	7.107780247336537E-5	0.0	0.0	10.944275713638906	0.0
7	1.4215560494673074E-4	0.0	0.0	12.820942932343172	0.0
8	1.4215560494673074E-4	0.0	0.0	15.337310373307727	0.0
9	1.4215560494673074E-4	0.0	0.0	16.239074453287312	0.0
10	1.4215560494673074E-4	0.0	0.0	18.80157138805708	0.0
11	1.4215560494673074E-4	0.0	0.0	21.920252127180934	0.0
12	1.4215560494673074E-4	0.0	0.0	25.147610826286563	0.0
13	1.4215560494673074E-4	0.0	0.0	26.076313393403552	0.0
14	1.4215560494673074E-4	0.0	0.0	26.443430243178486	0.0
15	1.4215560494673074E-4	0.0	0.0	27.210999432088357	0.0
16	1.4215560494673074E-4	0.0	0.0	28.596021491084358	0.0
17	2.1323340742009612E-4	0.0	0.0	30.34908441128744	0.0
18	2.1323340742009612E-4	0.0	0.0	32.2292344423129	0.0
19	2.1323340742009612E-4	0.0	0.0	33.53528906276099	0.0
20	2.843112098934615E-4	0.0	0.0	34.60152717766394	0.0
21	2.843112098934615E-4	0.0	0.0	35.75547530081903	0.0
22	2.843112098934615E-4	0.0	0.0	37.00701324677005	0.0
23	3.5538901236682686E-4	0.0	0.0	38.13537336103472	0.0
24	3.5538901236682686E-4	0.0	0.0	39.01325529938326	0.0
25	3.5538901236682686E-4	0.0	0.0	39.781819577527756	0.0
26	3.5538901236682686E-4	0.0	0.0	40.498212748656805	0.0
27	3.5538901236682686E-4	0.0	0.0	41.17387833896862	0.0
28	4.2646681484019225E-4	0.0	0.0	41.867597691108664	0.0
29	4.2646681484019225E-4	0.0	0.0	42.58768690796633	0.0
30	4.2646681484019225E-4	0.0	0.0	43.41524576216372	0.0
31	4.2646681484019225E-4	0.0	0.0	44.16696460112203	0.0
32	4.2646681484019225E-4	0.0	0.0	44.83886306790276	0.0
33	4.2646681484019225E-4	0.0	0.0	45.4912151390033	0.0
34	4.975446173135576E-4	0.0	0.0	46.15131469057345	0.0
35	5.68622419786923E-4	0.0	0.0	46.829823392984196	0.0
36	5.68622419786923E-4	0.0	0.0	47.472153493936	0.0
37	5.68622419786923E-4	0.0	0.0	48.09166762029385	0.0
38	5.68622419786923E-4	0.0	0.0	48.74494370282655	0.0
39	5.68622419786923E-4	0.0	0.0	49.3351026967629	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	65	0.0	45.000004	1
GTATACG	75	0.0	45.000004	1
CTATGCG	45	3.8562575E-10	45.0	1
AGCGTAA	45	3.8562575E-10	45.0	25
ATCTCGC	35	1.2121927E-7	45.0	37
TCTTGCG	55	1.8189894E-12	45.0	1
CGAACGA	20	7.0338266E-4	45.0	12
TCCGCGC	20	7.0338266E-4	45.0	14
CGTATAT	20	7.0338266E-4	45.0	41
GGTCGTC	20	7.0338266E-4	45.0	9
CCGGCCA	20	7.0338266E-4	45.0	22
GCAACGT	20	7.0338266E-4	45.0	36
CGTTGTC	20	7.0338266E-4	45.0	20
ATTACTC	35	1.2121927E-7	45.0	10
GAATCCG	20	7.0338266E-4	45.0	24
GCACGGT	35	1.2121927E-7	45.0	3
TATGCGT	20	7.0338266E-4	45.0	36
CATTCGA	45	3.8562575E-10	45.0	12
ATCCGCG	20	7.0338266E-4	45.0	1
ATATCGG	20	7.0338266E-4	45.0	33
>>END_MODULE