SRR2935000.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935000.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1478175
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	14430	0.9762037647775128	RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	14145	0.9569232330407428	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	9658	0.6533732474165779	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	6565	0.44412873983121076	Illumina PCR Primer Index 7 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	4735	0.3203274307845823	Illumina PCR Primer Index 7 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	3374	0.2282544353679368	Illumina PCR Primer Index 7 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2606	0.17629847616148292	RNA PCR Primer, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2588	0.17508075836758166	Illumina PCR Primer Index 7 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2322	0.15708559541326297	RNA PCR Primer, Index 16 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2038	0.13787271466504306	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	1801	0.1218394303786764	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1713	0.11588614338627022	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	1542	0.10431782434420823	Illumina PCR Primer Index 7 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	35	1.2122473E-7	45.0	23
AGCGTCG	20	7.033943E-4	45.0	2
AGCCGGT	25	3.8913495E-5	45.0	38
TACCCGC	20	7.033943E-4	45.0	39
CGCGGTC	35	1.2122473E-7	45.0	37
CCGATCA	20	7.033943E-4	45.0	10
GCGGTAT	35	1.2122473E-7	45.0	5
ATAATCG	25	3.8913495E-5	45.0	33
GACGTGC	25	3.8913495E-5	45.0	8
TTAGACG	20	7.033943E-4	45.0	36
TAGCGAT	20	7.033943E-4	45.0	23
GATTTCG	45	3.8562575E-10	45.0	13
GTTAGCG	90	0.0	45.0	1
CCTGTCG	20	7.033943E-4	45.0	12
CGCGACA	25	3.8913495E-5	45.0	33
TAACGCG	20	7.033943E-4	45.0	1
GTAATCG	35	1.2122473E-7	45.0	1
TACGCTT	20	7.033943E-4	45.0	40
ATATCGC	20	7.033943E-4	45.0	16
TCGCTTA	20	7.033943E-4	45.0	32