##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935000.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1478175
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11352647690564	33.0	31.0	34.0	30.0	34.0
2	32.293070170987875	34.0	31.0	34.0	30.0	34.0
3	32.309213049875694	34.0	31.0	34.0	30.0	34.0
4	35.881231247991614	37.0	35.0	37.0	35.0	37.0
5	35.77559152333114	37.0	35.0	37.0	35.0	37.0
6	35.70721463967392	37.0	35.0	37.0	33.0	37.0
7	36.04865188492567	37.0	35.0	37.0	35.0	37.0
8	35.85218123699833	37.0	35.0	37.0	35.0	37.0
9	37.62316775753886	39.0	38.0	39.0	35.0	39.0
10	37.32241649331101	39.0	37.0	39.0	34.0	39.0
11	37.250818746088925	39.0	37.0	39.0	34.0	39.0
12	37.22690547465625	39.0	37.0	39.0	34.0	39.0
13	37.1349677812167	39.0	37.0	39.0	33.0	39.0
14	38.23446750215638	40.0	37.0	41.0	33.0	41.0
15	38.3070847497759	40.0	37.0	41.0	34.0	41.0
16	38.34788709050011	40.0	37.0	41.0	34.0	41.0
17	38.391281140595666	40.0	38.0	41.0	34.0	41.0
18	38.429630456475046	40.0	38.0	41.0	34.0	41.0
19	38.42270976034637	40.0	37.0	41.0	34.0	41.0
20	38.391413736533224	40.0	37.0	41.0	34.0	41.0
21	38.35005530468314	40.0	37.0	41.0	34.0	41.0
22	38.27240076445617	40.0	37.0	41.0	34.0	41.0
23	38.04104182522367	40.0	37.0	41.0	34.0	41.0
24	37.822811236829196	39.0	36.0	41.0	33.0	41.0
25	37.75259559930319	39.0	36.0	41.0	33.0	41.0
26	37.9231633602246	40.0	37.0	41.0	34.0	41.0
27	37.991347438564446	40.0	37.0	41.0	34.0	41.0
28	37.96686928137738	40.0	37.0	41.0	34.0	41.0
29	38.014187765318724	40.0	37.0	41.0	34.0	41.0
30	37.94639876875201	40.0	37.0	41.0	34.0	41.0
31	37.79273969590881	40.0	36.0	41.0	33.0	41.0
32	37.7768532142676	40.0	36.0	41.0	34.0	41.0
33	37.741049605087355	40.0	36.0	41.0	33.0	41.0
34	37.638266781673344	40.0	36.0	41.0	33.0	41.0
35	37.64801461261353	40.0	36.0	41.0	33.0	41.0
36	37.58240803693744	40.0	36.0	41.0	33.0	41.0
37	37.50611057554079	39.0	36.0	41.0	33.0	41.0
38	37.4772520168451	39.0	36.0	41.0	33.0	41.0
39	37.41690530552878	39.0	36.0	41.0	33.0	41.0
40	37.35488220271619	39.0	36.0	41.0	33.0	41.0
41	37.37638980499602	39.0	36.0	41.0	33.0	41.0
42	37.39852047288041	39.0	36.0	41.0	33.0	41.0
43	37.320175892570234	39.0	36.0	41.0	33.0	41.0
44	37.16311262198319	39.0	35.0	41.0	32.0	41.0
45	37.170389162311636	39.0	35.0	41.0	32.0	41.0
46	37.03400409288481	39.0	35.0	41.0	32.0	41.0
47	37.00172848275746	39.0	35.0	41.0	32.0	41.0
48	37.01618685203037	39.0	35.0	41.0	32.0	41.0
49	36.9917229015509	39.0	35.0	41.0	32.0	41.0
50	36.914094068699576	39.0	35.0	41.0	32.0	41.0
51	35.85713193634042	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	12.0
10	14.0
11	12.0
12	20.0
13	17.0
14	16.0
15	34.0
16	42.0
17	83.0
18	209.0
19	353.0
20	728.0
21	1135.0
22	1918.0
23	2846.0
24	3955.0
25	5451.0
26	6855.0
27	8323.0
28	10468.0
29	13456.0
30	17794.0
31	23874.0
32	32614.0
33	45926.0
34	82129.0
35	115757.0
36	111582.0
37	179300.0
38	348815.0
39	464121.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.29607455138938	22.946606457286858	26.668087337426215	12.089231653897542
2	28.878853992253962	24.743010807245422	27.16051888308218	19.217616317418436
3	28.82953642160096	24.59600520912612	28.99961100681584	17.57484736245708
4	28.984998393289025	24.442911022037308	27.68914370761243	18.88294687706124
5	23.946691021022545	28.90943223907859	27.051194885585268	20.092681854313597
6	24.757420467806586	32.71344732524904	25.84233937118406	16.68679283576031
7	77.37642701303973	5.393542713142896	10.267525834221253	6.962504439596123
8	76.82933346863531	3.7420467806585824	10.572564141593519	8.856055609112587
9	72.27763965700949	5.904375327684476	13.273665161432172	8.544319853873866
10	36.21066517834492	25.112249902751703	21.50455798535356	17.17252693354982
11	23.86672755255636	26.809410252507316	27.481387521775165	21.84247467316116
12	23.282155360495203	22.09271567980787	33.55732575642262	21.06780320327431
13	21.611717151216872	25.826238435909143	33.47073249107852	19.091311921795455
14	16.585586956889408	29.788150929355456	30.72958208601823	22.896680027736906
15	14.822669846263128	27.195359142185467	37.33948957329139	20.642481438260017
16	16.719535914218547	27.817207028937712	31.76667174049081	23.696585316352937
17	16.708847058027633	27.144147343853064	28.671571363336547	27.47543423478276
18	17.352613865070104	26.99572107497421	33.64236304903005	22.009302010925637
19	18.288835895614525	27.079743602753396	30.64217700881154	23.98924349282054
20	19.58570534611937	29.355116951646455	30.48759449997463	20.571583202259543
21	20.151402912375058	28.186581426421093	31.454733032286438	20.207282628917415
22	17.990224432154513	26.21644933786595	28.073063067634074	27.72026316234546
23	16.83860165406667	26.392950766993085	29.93610364131446	26.83234393762579
24	21.07889796539652	24.798214013902278	29.543254350804197	24.579633669897003
25	17.105890709827996	28.022595430175723	27.855835743399798	27.01567811659648
26	17.046154886938286	30.433406058146023	28.42105975273564	24.09937930218005
27	22.049824953067127	27.634346406886873	28.47301571194209	21.84281292810391
28	15.630557951528068	26.614440103506016	32.073266020599725	25.681735924366194
29	17.506046307101663	25.653660764117912	29.573832597628837	27.26646033115159
30	21.924264718318195	26.047118913525125	26.63980922421229	25.38880714394439
31	18.46844927021496	30.460263500600405	25.556040387640167	25.51524684154447
32	18.67816733472018	28.445075853670907	23.337426218140614	29.5393305934683
33	19.669660222910007	24.81194716457794	24.586195815786358	30.93219679672569
34	16.12298949718403	26.41777867979096	28.018130464931417	29.441101358093597
35	16.99392832377763	25.806687300218172	23.599438496795035	33.59994587920916
36	20.505758790400325	25.260405567676358	25.70859336682057	28.525242275102748
37	18.331185414446868	25.60799634684662	30.788641399022442	25.272176839684068
38	16.46320631860233	24.52145381974394	26.00395758283018	33.01138227882355
39	18.801224482892756	23.889525935697737	27.208280480998532	30.10096910041098
40	19.919901229556718	24.53951663368681	27.817680585857563	27.722901550898914
41	17.47526510731138	26.314543271263553	24.131445870752785	32.07874575067228
42	20.004464965244303	22.48549731932958	27.574407631031512	29.93563008439461
43	22.83430581629374	21.545216229472153	28.56630642515264	27.054171529081465
44	19.69239095506283	22.55680146126135	27.17871699900215	30.572090584673667
45	19.987687520083888	21.66712331083938	26.344106753259933	32.0010824158168
46	22.129111911647808	22.89052378777885	28.002503086576354	26.977861213996988
47	16.758570534611934	23.58516413821097	31.81869535068581	27.837569976491285
48	17.361205540615963	20.235696044108444	29.81494072082128	32.58815769445431
49	20.079084005615034	18.62046104148697	33.016726706919	28.283728245978995
50	19.114110304936833	19.003027381737617	30.907707138870567	30.975155174454986
51	17.98413584318501	18.750147986537453	26.68229404502173	36.58342212525581
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	971.0
1	1107.5
2	1244.0
3	1795.0
4	2346.0
5	1761.0
6	1176.0
7	1261.0
8	1346.0
9	1527.5
10	1709.0
11	1854.0
12	1999.0
13	2073.5
14	2148.0
15	2324.0
16	2500.0
17	2477.0
18	2454.0
19	2545.0
20	2636.0
21	3050.0
22	3464.0
23	4217.5
24	4971.0
25	6372.0
26	8871.0
27	9969.0
28	11728.5
29	13488.0
30	17386.5
31	21285.0
32	22434.5
33	23584.0
34	28325.5
35	33067.0
36	36157.5
37	39248.0
38	42307.0
39	45366.0
40	50819.0
41	56272.0
42	64882.5
43	73493.0
44	82866.5
45	92240.0
46	104547.0
47	116854.0
48	142734.5
49	168615.0
50	201420.5
51	234226.0
52	224366.0
53	214506.0
54	165565.0
55	116624.0
56	91167.5
57	65711.0
58	53064.0
59	40417.0
60	34371.5
61	28326.0
62	23390.0
63	18454.0
64	15817.5
65	13181.0
66	9993.5
67	6806.0
68	5756.5
69	4707.0
70	3488.5
71	2270.0
72	1782.0
73	1294.0
74	845.5
75	465.0
76	533.0
77	363.0
78	193.0
79	207.0
80	221.0
81	139.0
82	57.0
83	42.5
84	28.0
85	15.5
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1478175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.88385937484307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.26237735081506	11.982486368373344
2	13.01109566881204	5.174215931825765
3	6.637929847296907	3.9596299087117566
4	4.075674824305299	3.241605802562992
5	2.829265491305494	2.812835858160736
6	2.0171344040834978	2.406505009857249
7	1.473980821306172	2.051589916044733
8	1.1390723451736462	1.8119323463364387
9	0.9097119014219753	1.627972516754609
>10	5.8302805056460185	22.855461259950395
>50	1.1830924912601921	17.013652104857893
>100	0.605292714200317	17.321767890981075
>500	0.017186050940677936	2.341704872828602
>1k	0.006530699357457616	2.336920722547015
>5k	6.874420376271174E-4	1.1087610002597088
>10k+	6.874420376271174E-4	1.952958489947678
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	14430	0.9762037647775128	RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	14145	0.9569232330407428	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	9658	0.6533732474165779	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	6565	0.44412873983121076	Illumina PCR Primer Index 7 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	4735	0.3203274307845823	Illumina PCR Primer Index 7 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	3374	0.2282544353679368	Illumina PCR Primer Index 7 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2606	0.17629847616148292	RNA PCR Primer, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2588	0.17508075836758166	Illumina PCR Primer Index 7 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2322	0.15708559541326297	RNA PCR Primer, Index 16 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2038	0.13787271466504306	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	1801	0.1218394303786764	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1713	0.11588614338627022	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	1542	0.10431782434420823	Illumina PCR Primer Index 7 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0295296565021057E-4	0.0	0.0	0.6848309570923605	0.0
2	2.0295296565021057E-4	0.0	0.0	3.5147394591303467	0.0
3	2.0295296565021057E-4	0.0	0.0	4.8906929152502245	0.0
4	2.0295296565021057E-4	0.0	0.0	6.315558036091803	0.0
5	2.0295296565021057E-4	0.0	0.0	9.982647521436906	0.0
6	2.0295296565021057E-4	0.0	0.0	11.719045444551558	0.0
7	2.0295296565021057E-4	0.0	0.0	13.598931114380909	0.0
8	2.0295296565021057E-4	0.0	0.0	16.467197726926784	0.0
9	2.0295296565021057E-4	0.0	0.0	17.425676932704178	0.0
10	2.0295296565021057E-4	0.0	0.0	19.773707443300015	0.0
11	2.0295296565021057E-4	0.0	0.0	23.40108579836623	0.0
12	2.0295296565021057E-4	0.0	0.0	25.998883758688923	0.0
13	2.0295296565021057E-4	0.0	0.0	27.03522925228745	0.0
14	2.0295296565021057E-4	0.0	0.0	27.40270942209143	0.0
15	2.0295296565021057E-4	0.0	0.0	28.11798332403132	0.0
16	2.0295296565021057E-4	0.0	0.0	29.651834187427063	0.0
17	2.0295296565021057E-4	0.0	0.0	31.544979451012228	0.0
18	2.0295296565021057E-4	0.0	0.0	33.57647098618229	0.0
19	2.0295296565021057E-4	0.0	0.0	34.748524362812255	0.0
20	2.0295296565021057E-4	0.0	0.0	35.85915064183875	0.0
21	2.0295296565021057E-4	0.0	0.0	37.22739188526393	0.0
22	2.0295296565021057E-4	0.0	0.0	38.62370828893737	0.0
23	2.0295296565021057E-4	0.0	0.0	40.01880697481692	0.0
24	2.0295296565021057E-4	0.0	0.0	41.087151385999626	0.0
25	2.0295296565021057E-4	0.0	0.0	41.973108732051344	0.0
26	2.0295296565021057E-4	0.0	0.0	42.781064488304835	0.0
27	2.0295296565021057E-4	0.0	0.0	43.50973328597764	0.0
28	2.0295296565021057E-4	0.0	0.0	44.25186463037191	0.0
29	2.0295296565021057E-4	0.0	0.0	45.02477717455646	0.0
30	2.0295296565021057E-4	0.0	0.0	45.90329291186767	0.0
31	2.7060395420028076E-4	0.0	0.0	46.71713430412502	0.0
32	2.7060395420028076E-4	0.0	0.0	47.46718081417965	0.0
33	2.7060395420028076E-4	0.0	0.0	48.16601552590187	0.0
34	2.7060395420028076E-4	0.0	0.0	48.87682446259746	0.0
35	2.7060395420028076E-4	0.0	0.0	49.62768278451469	0.0
36	2.7060395420028076E-4	0.0	0.0	50.32340555076361	0.0
37	2.7060395420028076E-4	0.0	0.0	50.996668188813906	0.0
38	2.7060395420028076E-4	0.0	0.0	51.669389618955805	0.0
39	2.7060395420028076E-4	0.0	0.0	52.28771965430345	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	35	1.2122473E-7	45.0	23
AGCGTCG	20	7.033943E-4	45.0	2
AGCCGGT	25	3.8913495E-5	45.0	38
TACCCGC	20	7.033943E-4	45.0	39
CGCGGTC	35	1.2122473E-7	45.0	37
CCGATCA	20	7.033943E-4	45.0	10
GCGGTAT	35	1.2122473E-7	45.0	5
ATAATCG	25	3.8913495E-5	45.0	33
GACGTGC	25	3.8913495E-5	45.0	8
TTAGACG	20	7.033943E-4	45.0	36
TAGCGAT	20	7.033943E-4	45.0	23
GATTTCG	45	3.8562575E-10	45.0	13
GTTAGCG	90	0.0	45.0	1
CCTGTCG	20	7.033943E-4	45.0	12
CGCGACA	25	3.8913495E-5	45.0	33
TAACGCG	20	7.033943E-4	45.0	1
GTAATCG	35	1.2122473E-7	45.0	1
TACGCTT	20	7.033943E-4	45.0	40
ATATCGC	20	7.033943E-4	45.0	16
TCGCTTA	20	7.033943E-4	45.0	32
>>END_MODULE