SRR2934999.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934999.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283443
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	12185	0.9493993889872787	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	11329	0.8827037897280985	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	10180	0.7931789724981942	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	9526	0.7422222880174656	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	9119	0.7105107122014769	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	6356	0.49523040758335196	No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	4111	0.32031029036739456	No Hit
ACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	3202	0.2494851738643633	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT	2471	0.19252900206709608	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCGTAAT	2414	0.18808782314446376	No Hit
AGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	2091	0.16292114258288057	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	2011	0.15668790900725627	No Hit
ACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1846	0.14383186475753112	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT	1665	0.1297291737926811	No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1655	0.12895001959572805	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTA	1617	0.1259892336473065	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTC	1602	0.12482050235187694	No Hit
GGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1584	0.12341802479736148	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC	1524	0.11874309961564322	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	1293	0.10074463766602802	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	1.2120836E-7	45.000004	1
TTTCGAT	35	1.2120836E-7	45.000004	16
TACGGGT	70	0.0	45.000004	4
TCGATAG	35	1.2120836E-7	45.000004	1
CGTACAC	35	1.2120836E-7	45.000004	21
CCTATAG	35	1.2120836E-7	45.000004	2
TTACCGG	35	1.2120836E-7	45.000004	2
AATCGGC	35	1.2120836E-7	45.000004	19
AACCGCA	20	7.0335955E-4	45.0	13
CGAACGT	20	7.0335955E-4	45.0	37
ACGTTCG	20	7.0335955E-4	45.0	40
TTAGCGA	25	3.8910617E-5	45.0	2
GCGCGCA	20	7.0335955E-4	45.0	36
CGCATAT	20	7.0335955E-4	45.0	45
CGCATAC	20	7.0335955E-4	45.0	11
TAGCGAA	45	3.8562575E-10	45.0	1
CACGCAT	20	7.0335955E-4	45.0	9
CGTTCTA	20	7.0335955E-4	45.0	40
CGCGACA	20	7.0335955E-4	45.0	38
ATCACCG	25	3.8910617E-5	45.0	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content