##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934999.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283443
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0900110094488	33.0	31.0	34.0	30.0	34.0
2	32.267226514928986	34.0	31.0	34.0	30.0	34.0
3	32.27773886335427	34.0	31.0	34.0	30.0	34.0
4	35.864840900608755	37.0	35.0	37.0	35.0	37.0
5	35.765803389788246	37.0	35.0	37.0	35.0	37.0
6	35.69378850482647	37.0	35.0	37.0	33.0	37.0
7	36.00322803583798	37.0	35.0	37.0	35.0	37.0
8	35.670533089510016	37.0	35.0	37.0	35.0	37.0
9	37.43658035456191	39.0	37.0	39.0	35.0	39.0
10	37.269348930961485	39.0	37.0	39.0	34.0	39.0
11	37.24455546526024	39.0	37.0	39.0	34.0	39.0
12	37.164015854229596	39.0	37.0	39.0	34.0	39.0
13	37.04882413944367	39.0	37.0	39.0	33.0	39.0
14	38.13472199388676	40.0	37.0	41.0	33.0	41.0
15	38.16758749706843	40.0	37.0	41.0	33.0	41.0
16	38.276256912071666	40.0	37.0	41.0	34.0	41.0
17	38.26500280885088	40.0	37.0	41.0	34.0	41.0
18	38.268857284663206	40.0	37.0	41.0	34.0	41.0
19	38.27082932393569	40.0	37.0	41.0	34.0	41.0
20	38.21298803297069	40.0	37.0	41.0	34.0	41.0
21	38.17990124999708	40.0	37.0	41.0	34.0	41.0
22	38.10857903311639	40.0	37.0	41.0	34.0	41.0
23	37.84495376888572	40.0	36.0	41.0	33.0	41.0
24	37.588962657476806	39.0	36.0	41.0	33.0	41.0
25	37.559187279840245	39.0	36.0	41.0	33.0	41.0
26	37.73229898016507	39.0	36.0	41.0	33.0	41.0
27	37.783060096942364	40.0	36.0	41.0	33.0	41.0
28	37.80317240422831	40.0	36.0	41.0	33.0	41.0
29	37.846241710773285	40.0	36.0	41.0	33.0	41.0
30	37.79234216089067	40.0	36.0	41.0	33.0	41.0
31	37.68213235804005	40.0	36.0	41.0	33.0	41.0
32	37.55688643749664	40.0	36.0	41.0	33.0	41.0
33	37.499507184970426	40.0	36.0	41.0	33.0	41.0
34	37.34872526477608	39.0	36.0	41.0	33.0	41.0
35	37.26233576403471	39.0	36.0	41.0	32.0	41.0
36	37.211940070575785	39.0	35.0	41.0	32.0	41.0
37	37.19130572997788	39.0	35.0	41.0	32.0	41.0
38	37.13817988021283	39.0	35.0	41.0	32.0	41.0
39	37.08579500608909	39.0	35.0	41.0	32.0	41.0
40	36.97462061034265	39.0	35.0	41.0	31.0	41.0
41	36.8596127759472	39.0	35.0	41.0	31.0	41.0
42	36.92490044357248	39.0	35.0	41.0	31.0	41.0
43	36.89611459176606	39.0	35.0	41.0	31.0	41.0
44	36.839179457132104	39.0	35.0	41.0	31.0	41.0
45	36.86269822656713	39.0	35.0	41.0	31.0	41.0
46	36.72451990466269	39.0	35.0	41.0	31.0	41.0
47	36.5569729236125	39.0	35.0	40.0	31.0	41.0
48	36.57511630824275	39.0	35.0	40.0	31.0	41.0
49	36.57909544872659	39.0	35.0	40.0	31.0	41.0
50	36.51631665761549	39.0	35.0	40.0	31.0	41.0
51	35.47551858555464	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	11.0
11	13.0
12	16.0
13	15.0
14	16.0
15	30.0
16	65.0
17	132.0
18	250.0
19	460.0
20	849.0
21	1533.0
22	2383.0
23	3594.0
24	4927.0
25	6169.0
26	7312.0
27	8657.0
28	10615.0
29	13488.0
30	17230.0
31	23061.0
32	31096.0
33	43430.0
34	75256.0
35	104776.0
36	98477.0
37	157906.0
38	294434.0
39	376952.0
40	279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.141456223611016	22.1085003385425	23.36675645120196	11.383286986644519
2	30.394103984360815	26.83726507527019	24.83016386391916	17.93846707644983
3	29.37512612558563	27.591797999599514	26.553107539641417	16.479968335173435
4	28.254702390367157	24.433496462250368	28.46608692400052	18.845714223381947
5	25.508183846107695	28.92251545257561	24.765416150152365	20.803884551164327
6	24.487024355581042	33.62050359852366	26.023983924490608	15.868488121404688
7	74.78571311698299	5.793868523962498	13.195755479596679	6.224662879457833
8	73.00776115495586	6.94545842705909	11.32516208355182	8.72161833443324
9	66.77452757933153	7.4864251860035855	13.278423739893396	12.460623494771486
10	35.95687537350704	29.594613862867302	20.250451325068585	14.198059438557067
11	27.32143149325681	23.765060076684357	27.304445931763233	21.6090624982956
12	24.39017548889978	20.631613558218014	33.41776767647648	21.56044327640573
13	22.195687693181544	25.030562323375484	34.377373985443846	18.396375997999133
14	17.56969339503196	30.196198818334747	29.486155598651443	22.747952187981856
15	14.921504110427966	25.975208871761346	38.406458253307704	20.696828764502982
16	16.58975116152412	27.16139322120266	30.842117647608813	25.406737969664412
17	17.21525615083802	26.832200572989994	29.060659491695382	26.8918837844766
18	17.781623336603182	26.82744773238858	32.755019116548226	22.63590981446001
19	18.30848740458283	27.41913742955472	29.250149792394364	25.02222537346809
20	20.96750693252447	28.18372144302474	30.660263058039977	20.188508566410817
21	20.158043637309955	29.203400540577185	30.588580871920296	20.049974950192567
22	18.189354727868707	25.43143715770782	28.86197517147236	27.517232942951107
23	17.662646490728452	27.695269676954876	30.050341152665133	24.59174267965153
24	21.374926662111214	24.24369449987261	28.80353860670088	25.577840231315296
25	16.938656410919688	30.69205254927566	26.533472853878198	25.835818185926453
26	17.216970290071316	29.117459832653264	27.95340346240542	25.71216641487
27	19.788958294213298	27.65958441473443	27.561956393856214	24.989500897196056
28	14.681446702346735	26.298090370978688	29.943285366003785	29.07717756067079
29	17.668723893464687	25.677571968525285	27.212116159424298	29.441587978585726
30	20.197624670515168	26.02686679501933	26.258976830291648	27.51653170417385
31	19.634374101537816	26.628451750486775	24.7996210193986	28.937553128576806
32	19.63149123100909	30.578841444458384	22.843943985046472	26.945723339486054
33	21.44863464914297	26.32208832024484	23.069353294224985	29.1599237363872
34	18.288930634239307	25.98775325433229	28.626982265671323	27.096333845757076
35	16.922138341944283	29.084189948443367	24.91664997978095	29.077021729831397
36	20.052156581944036	30.028992327668625	24.39710996125266	25.52174112913468
37	17.391500830188797	25.380402557807397	29.98613884683621	27.2419577651676
38	19.017907300908572	27.882422515063	23.289386439444527	29.810283744583906
39	22.117538527227154	24.85587595241861	28.17990358745967	24.846681932894565
40	21.892129218048638	25.35515796182612	30.3927015068063	22.36001131331894
41	17.15549502393172	29.62320882189548	26.52451258061324	26.696783573559557
42	18.848597093910676	27.366077028742218	27.537179290393105	26.248146586953997
43	21.9542278075458	25.27786586548838	25.538103367270693	27.22980295969513
44	19.785919592845183	22.596250865835103	27.379634311769202	30.238195229550513
45	20.277643806542244	21.71043045931919	26.785217574913727	31.226708159224835
46	24.356048535073235	23.839079725394896	27.21157075148643	24.59330098804544
47	16.270375856193066	24.744145240575545	33.84303003717345	25.142448866057936
48	18.09803785598581	23.97091261551935	28.987574828021188	28.943474700473647
49	19.65104800135261	20.954962549953525	33.737922136004485	25.656067312689384
50	19.674734288939984	20.79289847698729	31.18377676297272	28.348590471100003
51	18.536078345512813	20.17440587544597	26.998472078619773	34.29104370042144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1384.0
1	1457.0
2	1530.0
3	2156.5
4	2783.0
5	2138.5
6	1494.0
7	1558.5
8	1623.0
9	1840.5
10	2058.0
11	2217.5
12	2377.0
13	2503.5
14	2630.0
15	2640.0
16	2650.0
17	2700.5
18	2751.0
19	3083.5
20	3416.0
21	3374.5
22	3333.0
23	3847.5
24	4362.0
25	5133.0
26	7630.5
27	9357.0
28	10770.0
29	12183.0
30	13989.5
31	15796.0
32	17936.5
33	20077.0
34	23615.0
35	27153.0
36	28000.5
37	28848.0
38	33034.0
39	37220.0
40	43337.0
41	49454.0
42	60002.0
43	70550.0
44	84479.0
45	98408.0
46	135962.5
47	173517.0
48	180418.5
49	187320.0
50	181110.5
51	174901.0
52	140106.5
53	105312.0
54	86030.5
55	66749.0
56	55761.0
57	44773.0
58	40025.0
59	35277.0
60	31683.5
61	28090.0
62	24684.0
63	21278.0
64	17933.0
65	14588.0
66	11570.0
67	8552.0
68	7320.5
69	6089.0
70	4687.0
71	3285.0
72	3179.5
73	3074.0
74	2430.5
75	1293.5
76	800.0
77	537.5
78	275.0
79	255.5
80	236.0
81	191.5
82	147.0
83	85.0
84	23.0
85	17.5
86	12.0
87	10.0
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1283443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.85690560317822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.27870779830709	12.36662421838903
2	12.689141350673323	5.03934163971411
3	6.3443867744317926	3.7793966786983337
4	3.975513809507748	3.1576560975810715
5	2.64215558093056	2.6232516979724316
6	1.8641791768149303	2.2210097964854727
7	1.410626795501072	1.9607478183705
8	1.036830140783985	1.6470590585662062
9	0.811460617659254	1.4501787197000853
>10	5.017414247661186	19.39511193715073
>50	1.2147174157601708	17.644615599120915
>100	0.6890803269423321	19.186295785425127
>500	0.015074871861719111	1.9813965146072203
>1k	0.008330850239371088	2.9236882932313035
>5k	0.0011901214627672984	1.9694229023056935
>10k+	0.0011901214627672984	2.6542032426818145
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	12185	0.9493993889872787	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	11329	0.8827037897280985	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	10180	0.7931789724981942	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	9526	0.7422222880174656	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	9119	0.7105107122014769	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	6356	0.49523040758335196	No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	4111	0.32031029036739456	No Hit
ACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	3202	0.2494851738643633	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT	2471	0.19252900206709608	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCGTAAT	2414	0.18808782314446376	No Hit
AGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	2091	0.16292114258288057	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	2011	0.15668790900725627	No Hit
ACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1846	0.14383186475753112	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT	1665	0.1297291737926811	No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1655	0.12895001959572805	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTA	1617	0.1259892336473065	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTC	1602	0.12482050235187694	No Hit
GGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1584	0.12341802479736148	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC	1524	0.11874309961564322	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	1293	0.10074463766602802	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.518059625554076	0.0
2	0.0	0.0	0.0	2.8626904350251627	0.0
3	0.0	0.0	0.0	3.95779165884266	0.0
4	0.0	0.0	0.0	5.353880148943117	0.0
5	0.0	0.0	0.0	9.451374155299456	0.0
6	0.0	0.0	0.0	10.944467342920566	0.0
7	0.0	0.0	0.0	12.725769667994605	0.0
8	0.0	0.0	0.0	15.202467113849233	0.0
9	0.0	0.0	0.0	16.042784915263084	0.0
10	0.0	0.0	0.0	18.718088765921042	0.0
11	0.0	0.0	0.0	21.630878815810284	0.0
12	0.0	0.0	0.0	24.972749081961567	0.0
13	7.791541969530396E-5	0.0	0.0	25.918876023321644	0.0
14	7.791541969530396E-5	0.0	0.0	26.29551916212874	0.0
15	7.791541969530396E-5	0.0	0.0	27.12866874493063	0.0
16	7.791541969530396E-5	0.0	0.0	28.489617380748502	0.0
17	7.791541969530396E-5	0.0	0.0	30.10036285210952	0.0
18	7.791541969530396E-5	0.0	0.0	31.772349843351048	0.0
19	3.895770984765198E-4	0.0	0.0	32.97863637107375	0.0
20	3.895770984765198E-4	0.0	0.0	33.97790162866602	0.0
21	3.895770984765198E-4	0.0	0.0	35.16541054024214	0.0
22	3.895770984765198E-4	0.0	0.0	36.33079147262481	0.0
23	3.895770984765198E-4	0.0	0.0	37.4791089280942	0.0
24	3.895770984765198E-4	0.0	0.0	38.39562800997006	0.0
25	3.895770984765198E-4	0.0	0.0	39.15732915291135	0.0
26	3.895770984765198E-4	0.0	0.0	39.90290180397571	0.0
27	3.895770984765198E-4	0.0	0.0	40.605309312528874	0.0
28	3.895770984765198E-4	0.0	0.0	41.288939204935474	0.0
29	3.895770984765198E-4	0.0	0.0	42.00217695682629	0.0
30	3.895770984765198E-4	0.0	0.0	42.826677928041995	0.0
31	5.454079378671277E-4	0.0	0.0	43.507580780759255	0.0
32	5.454079378671277E-4	0.0	0.0	44.164875261308836	0.0
33	5.454079378671277E-4	0.0	0.0	44.80658665791936	0.0
34	5.454079378671277E-4	0.0	0.0	45.452349656354045	0.0
35	5.454079378671277E-4	0.0	0.0	46.17361269647347	0.0
36	5.454079378671277E-4	0.0	0.0	46.77636638323634	0.0
37	6.233233575624317E-4	0.0	0.0	47.40202720338963	0.0
38	6.233233575624317E-4	0.0	0.0	47.98787324407862	0.0
39	6.233233575624317E-4	0.0	0.0	48.618286904833326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	1.2120836E-7	45.000004	1
TTTCGAT	35	1.2120836E-7	45.000004	16
TACGGGT	70	0.0	45.000004	4
TCGATAG	35	1.2120836E-7	45.000004	1
CGTACAC	35	1.2120836E-7	45.000004	21
CCTATAG	35	1.2120836E-7	45.000004	2
TTACCGG	35	1.2120836E-7	45.000004	2
AATCGGC	35	1.2120836E-7	45.000004	19
AACCGCA	20	7.0335955E-4	45.0	13
CGAACGT	20	7.0335955E-4	45.0	37
ACGTTCG	20	7.0335955E-4	45.0	40
TTAGCGA	25	3.8910617E-5	45.0	2
GCGCGCA	20	7.0335955E-4	45.0	36
CGCATAT	20	7.0335955E-4	45.0	45
CGCATAC	20	7.0335955E-4	45.0	11
TAGCGAA	45	3.8562575E-10	45.0	1
CACGCAT	20	7.0335955E-4	45.0	9
CGTTCTA	20	7.0335955E-4	45.0	40
CGCGACA	20	7.0335955E-4	45.0	38
ATCACCG	25	3.8910617E-5	45.0	38
>>END_MODULE