FastQCFastQC Report
Sat 14 Jan 2017
SRR2934998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962057
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC158541.6479273057625483TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC131321.3649918871750841TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT97441.0128298011448387TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC59950.6231439509301424TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC52450.5451859920981813TruSeq Adapter, Index 27 (100% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG26450.2749317348140495TruSeq Adapter, Index 27 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG23250.2416696723790794TruSeq Adapter, Index 27 (100% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG23000.23907107375134737TruSeq Adapter, Index 27 (100% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG21830.22690963217356142TruSeq Adapter, Index 27 (100% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG20860.21682706949796116TruSeq Adapter, Index 27 (95% over 24bp)
AAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC20050.2084076099441093TruSeq Adapter, Index 27 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19810.2059129552614866No Hit
AGGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC17030.17701653852110633TruSeq Adapter, Index 27 (100% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC16390.17036412603411233TruSeq Adapter, Index 27 (100% over 21bp)
GAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC14480.15051083251823955TruSeq Adapter, Index 27 (100% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG14410.1497832249024746TruSeq Adapter, Index 27 (100% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT13960.14510574737255694TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC13150.13668628781870512No Hit
GACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT12250.1273313327588698TruSeq Adapter, Index 27 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC12100.12577217358223058TruSeq Adapter, Index 27 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG11930.12400512651537278TruSeq Adapter, Index 27 (95% over 24bp)
GTACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC11040.11475411540064676TruSeq Adapter, Index 27 (100% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT10920.11350678805933537TruSeq Adapter, Index 27 (100% over 22bp)
AGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT10810.11236340466313326TruSeq Adapter, Index 27 (95% over 23bp)
AGGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC10450.10862142263919913TruSeq Adapter, Index 27 (100% over 21bp)
AGACATAGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC10280.10685437557234134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTC302.1651304E-645.00000411
CGGGTAT302.1651304E-645.0000046
AACCGCA302.1651304E-645.00000418
CGAACAT351.211647E-745.00000440
CGCGGTC302.1651304E-645.00000422
GAATGCG302.1651304E-645.0000041
CAACGAC351.211647E-745.00000420
TAGACTT302.1651304E-645.00000420
TTAGCGA351.211647E-745.00000436
TAGACGC302.1651304E-645.0000042
TCACACG351.211647E-745.00000426
TTTCGTC302.1651304E-645.00000412
GTTTACG302.1651304E-645.0000041
GGCGTAT302.1651304E-645.0000047
ATAGCCG302.1651304E-645.0000041
TGTATCC302.1651304E-645.00000422
CGCGAGC302.1651304E-645.00000413
TCTAGCG600.045.0000041
AGTATCC302.1651304E-645.00000437
GTTACGG1200.045.0000042
Produced by FastQC (version 0.11.5)