SRR2934997.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934997.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334127
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	24229	1.8160939700643193	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	20442	1.5322379353689717	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	19398	1.4539845157170195	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	11444	0.8577894008591386	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	8244	0.617932175872312	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	5689	0.4264211727968927	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAAGAT	5505	0.41262938236015007	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	4901	0.3673563311438866	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	3948	0.2959238513274973	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTA	3565	0.2672159397118865	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	3533	0.2648173674620182	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	2857	0.2141475286835511	No Hit
ACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2682	0.20103033669208403	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	2347	0.17592028345127564	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	1946	0.14586317494511392	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1703	0.12764901692267677	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1693	0.12689946309459293	No Hit
TTGGCAGGGAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTTTG	1609	0.12060321093868874	No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1495	0.11205829729853305	No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1481	0.11100892193921569	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGC	20	7.0336944E-4	45.000004	42
GGTACGA	25	3.891143E-5	45.000004	8
GTCGCAC	20	7.0336944E-4	45.000004	25
TACCCGT	25	3.891143E-5	45.000004	18
TCGCAAT	20	7.0336944E-4	45.000004	30
TGCCCGT	25	3.891143E-5	45.000004	42
GTCGACG	25	3.891143E-5	45.000004	1
CAACGTT	25	3.891143E-5	45.000004	43
ACGCATA	20	7.0336944E-4	45.000004	10
CGACGAT	20	7.0336944E-4	45.000004	31
TCCGATC	25	3.891143E-5	45.000004	34
CGCATTC	20	7.0336944E-4	45.000004	23
CGCGCGA	20	7.0336944E-4	45.000004	45
CGTCTCG	25	3.891143E-5	45.000004	41
GTCTACG	20	7.0336944E-4	45.000004	1
GCGCATC	20	7.0336944E-4	45.000004	43
CGTTACC	20	7.0336944E-4	45.000004	15
ATCCGGC	460	0.0	45.000004	6
CGCCTCG	20	7.0336944E-4	45.000004	40
TCGGATA	20	7.0336944E-4	45.000004	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content