##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934997.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334127
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.125497797436076	33.0	31.0	34.0	30.0	34.0
2	32.299270609169895	34.0	31.0	34.0	30.0	34.0
3	32.32455231023733	34.0	31.0	34.0	30.0	34.0
4	35.88437532558744	37.0	35.0	37.0	35.0	37.0
5	35.79598718862597	37.0	35.0	37.0	35.0	37.0
6	35.70973528007453	37.0	35.0	37.0	33.0	37.0
7	36.017966055705344	37.0	35.0	37.0	35.0	37.0
8	35.60560876138479	37.0	35.0	37.0	35.0	37.0
9	37.46874547925347	39.0	37.0	39.0	35.0	39.0
10	37.28418958614885	39.0	37.0	39.0	34.0	39.0
11	37.24503514283123	39.0	37.0	39.0	34.0	39.0
12	37.20514838542358	39.0	37.0	39.0	34.0	39.0
13	37.108611848797004	39.0	37.0	39.0	33.0	39.0
14	38.2271387956319	40.0	37.0	41.0	33.0	41.0
15	38.20967868876051	40.0	37.0	41.0	33.0	41.0
16	38.36125871075242	40.0	38.0	41.0	34.0	41.0
17	38.304114226006966	40.0	37.0	41.0	34.0	41.0
18	38.33096099546745	40.0	37.0	41.0	34.0	41.0
19	38.33220375571441	40.0	37.0	41.0	34.0	41.0
20	38.218508432855344	40.0	37.0	41.0	34.0	41.0
21	38.24682058004973	40.0	37.0	41.0	34.0	41.0
22	38.21317235915321	40.0	37.0	41.0	34.0	41.0
23	37.93770832911709	40.0	37.0	41.0	33.0	41.0
24	37.69802649972604	40.0	36.0	41.0	33.0	41.0
25	37.712306249704866	39.0	36.0	41.0	33.0	41.0
26	37.91791036385592	40.0	37.0	41.0	33.0	41.0
27	37.8821109234728	40.0	37.0	41.0	33.0	41.0
28	37.86872913898002	40.0	37.0	41.0	33.0	41.0
29	37.94290873357634	40.0	37.0	41.0	34.0	41.0
30	37.80812846153327	40.0	36.0	41.0	33.0	41.0
31	37.773486332260724	40.0	37.0	41.0	33.0	41.0
32	37.60690998683034	40.0	36.0	41.0	33.0	41.0
33	37.50051007138001	40.0	36.0	41.0	33.0	41.0
34	37.43624782348307	40.0	36.0	41.0	33.0	41.0
35	37.39827017967555	40.0	36.0	41.0	33.0	41.0
36	37.37356638460956	40.0	36.0	41.0	33.0	41.0
37	37.29168662353734	40.0	36.0	41.0	32.0	41.0
38	37.2137165352324	40.0	36.0	41.0	32.0	41.0
39	37.18176305554119	39.0	36.0	41.0	32.0	41.0
40	37.12516049821344	39.0	36.0	41.0	32.0	41.0
41	37.06346996950065	39.0	35.0	41.0	32.0	41.0
42	37.203162067779154	39.0	36.0	41.0	32.0	41.0
43	37.14405824932709	39.0	36.0	41.0	32.0	41.0
44	37.07672657850414	39.0	35.0	41.0	32.0	41.0
45	37.096409862029624	39.0	35.0	41.0	32.0	41.0
46	36.99704675791735	39.0	35.0	41.0	32.0	41.0
47	36.8513215008766	39.0	35.0	41.0	31.0	41.0
48	36.883400905610934	39.0	35.0	41.0	31.0	41.0
49	36.89737034030493	39.0	35.0	41.0	31.0	41.0
50	36.84062386864219	39.0	35.0	41.0	31.0	41.0
51	35.86784466546288	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	16.0
11	5.0
12	18.0
13	8.0
14	7.0
15	19.0
16	48.0
17	92.0
18	186.0
19	471.0
20	961.0
21	1839.0
22	2929.0
23	4318.0
24	5383.0
25	6297.0
26	7377.0
27	8507.0
28	10196.0
29	12632.0
30	16787.0
31	22796.0
32	31128.0
33	43059.0
34	71199.0
35	101258.0
36	100053.0
37	157488.0
38	297908.0
39	430811.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07698817279015	20.74899915825105	24.70199613679957	10.472016532159232
2	27.36508593259862	30.741975838881906	24.5815428366265	17.311395391892976
3	28.182399426741235	30.77083366126313	26.116853942690614	14.92991296930502
4	26.55706690592425	23.92860649698267	32.8054975275967	16.70882906949638
5	29.244517201136023	26.974568388166944	23.63013416263969	20.150780248057345
6	23.01894797122013	37.265117938547085	24.158794477587218	15.557139612645573
7	74.36728287486874	5.346567455721981	14.553112259927278	5.733037409482006
8	73.81771000811767	8.98475182647529	9.543544205311788	7.653993960095254
9	68.59541857709198	6.748008248090325	10.866131934965711	13.790441239851978
10	37.05486808976956	31.517464229417442	17.82581418410691	13.601853496706084
11	25.89266239271074	23.1858736087344	30.208818200965876	20.712645797588987
12	22.294204374845872	19.707044381831714	34.76520601112188	23.233545232200534
13	22.040855180953535	22.887176408242993	37.51187105875228	17.56009735205119
14	17.9347993107103	30.44702640753092	29.898952648436016	21.719221633322764
15	14.334842185189267	25.180961032945138	39.871316598794564	20.612880183071027
16	16.815940311529562	27.049223949444094	30.429786669484987	25.705049069541356
17	17.19476481624313	26.796099621700183	31.70147969421202	24.307655867844666
18	17.825214541044442	24.826571983027105	33.545606977446674	23.80260649848178
19	17.83788200073906	27.346272131513714	30.23122986042558	24.584616007321642
20	20.15565234793989	27.902665938100345	32.806996635252865	19.1346850787069
21	19.8857380144469	30.143756928688198	30.51785924428484	19.45264581258006
22	17.643447737734114	24.894556515234306	30.873597491093424	26.588398255938152
23	16.96997362320079	28.91981048281011	30.114674240158546	23.995541653830557
24	19.962042594145835	24.496393521756175	29.41579025085318	26.12577363324481
25	16.259021817263275	31.856712292008183	26.995405984587673	24.88885990614087
26	17.023566721908782	27.071860475052222	29.37269090573836	26.53188189730063
27	20.1593251616975	30.27627804549342	27.178821806319785	22.385574986489292
28	16.346944481297506	27.048174574084776	31.539800933494337	25.06508001112338
29	18.599353734689426	23.151394132642544	28.47884796574839	29.77040416691964
30	18.332362661125963	32.639171533144896	26.91617814495921	22.112287660769926
31	24.1461270178926	28.357195379450385	21.877752267962496	25.618925334694527
32	22.041529779398815	30.887089459998933	22.07136202175655	25.000018738845704
33	19.76805806343774	31.19568077102105	22.793856956646554	26.242404208894655
34	21.488733831186984	27.685520194104456	24.586789713423084	26.238956261285466
35	19.99292421186289	27.78431138864591	24.369194237130348	27.853570162360853
36	21.343695165452765	30.83229707516601	23.66056604805989	24.163441711321337
37	20.03797239693073	30.12839107521248	27.774792055029245	22.05884447282755
38	22.50175582984229	29.13530720838421	23.05470168881973	25.308235272953773
39	20.79217345874868	29.366169787434032	25.539547584300443	24.302109169516843
40	22.13897177704971	27.932723046606505	27.644819421239507	22.283485755104273
41	19.67983557787227	31.94635892984701	22.862516087299035	25.511289404981685
42	22.72729657671271	25.004965794111055	28.498861052958226	23.768876576218005
43	22.667257315083198	26.46014959595301	25.87159993014158	25.00099315882221
44	20.364328133678427	24.513033616739634	27.91233518248263	27.21030306709931
45	20.349187146351134	23.10949407365266	26.419223956939632	30.122094823056578
46	24.943127603294137	26.458350666765607	25.477859304249144	23.12066242569111
47	17.44803905475266	25.469614212140222	33.68982113397001	23.392525599137112
48	19.657124096881333	26.214146029575897	26.995331029204866	27.133398844337908
49	20.604485180196487	21.723868867056883	33.43309894785129	24.238547004895334
50	21.36303365421733	22.197511931023058	29.577319100805244	26.862135313954365
51	19.870746937885222	21.899114552062883	26.10291224148825	32.12722626856363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	992.0
1	1060.0
2	1128.0
3	1759.0
4	2390.0
5	1826.5
6	1263.0
7	1350.0
8	1437.0
9	1672.0
10	1907.0
11	1944.5
12	1982.0
13	2252.5
14	2523.0
15	2462.0
16	2401.0
17	2523.5
18	2646.0
19	2724.5
20	2803.0
21	3295.5
22	3788.0
23	4337.5
24	4887.0
25	6307.0
26	9439.5
27	11152.0
28	14006.5
29	16861.0
30	19349.5
31	21838.0
32	24792.5
33	27747.0
34	30670.5
35	33594.0
36	35984.0
37	38374.0
38	41124.5
39	43875.0
40	49804.5
41	55734.0
42	65227.5
43	74721.0
44	86807.0
45	98893.0
46	155228.0
47	211563.0
48	199077.0
49	186591.0
50	175146.0
51	163701.0
52	132377.5
53	101054.0
54	82700.5
55	64347.0
56	54538.0
57	44729.0
58	38425.5
59	32122.0
60	28986.0
61	25850.0
62	20570.0
63	15290.0
64	13647.5
65	12005.0
66	9637.0
67	7269.0
68	5219.5
69	3170.0
70	2803.5
71	2437.0
72	2029.0
73	1621.0
74	1290.5
75	668.0
76	376.0
77	282.5
78	189.0
79	171.0
80	153.0
81	85.0
82	17.0
83	13.5
84	10.0
85	6.5
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1334127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.781582191285572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.90790180204192	12.44417829978414
2	12.988600111526535	5.138701213118061
3	6.270266692537814	3.7210738781915125
4	3.9080198544412768	3.09227263823224
5	2.617468600368502	2.5888835125649368
6	1.776283135278884	2.10826145013082
7	1.3081252509167471	1.8113751017253987
8	1.0025620985191293	1.5865811642979115
9	0.7373223677405271	1.3126862717039267
>10	4.577666571250658	17.972743962352528
>50	1.193079585760266	17.40854425018481
>100	0.6927681553627463	19.191737381460307
>500	0.00958450685338376	1.2473946749790652
>1k	0.007667605482707008	3.174607313632275
>5k	0.0011501408224060513	1.4741523402385133
>10k+	0.0015335210965414018	5.726806547403584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	24229	1.8160939700643193	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	20442	1.5322379353689717	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	19398	1.4539845157170195	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	11444	0.8577894008591386	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	8244	0.617932175872312	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	5689	0.4264211727968927	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAAGAT	5505	0.41262938236015007	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	4901	0.3673563311438866	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	3948	0.2959238513274973	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTA	3565	0.2672159397118865	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	3533	0.2648173674620182	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	2857	0.2141475286835511	No Hit
ACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2682	0.20103033669208403	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	2347	0.17592028345127564	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	1946	0.14586317494511392	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1703	0.12764901692267677	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1693	0.12689946309459293	No Hit
TTGGCAGGGAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTTTG	1609	0.12060321093868874	No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1495	0.11205829729853305	No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1481	0.11100892193921569	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.495538280838332E-5	0.0	0.0	0.4468839923035813	0.0
2	7.495538280838332E-5	0.0	0.0	2.52772037444711	0.0
3	7.495538280838332E-5	0.0	0.0	3.4014003164616264	0.0
4	7.495538280838332E-5	0.0	0.0	4.547468119601807	0.0
5	7.495538280838332E-5	0.0	0.0	8.444773248723697	0.0
6	7.495538280838332E-5	0.0	0.0	9.7117440843338	0.0
7	7.495538280838332E-5	0.0	0.0	11.43601771045785	0.0
8	7.495538280838332E-5	0.0	0.0	13.735723810401858	0.0
9	7.495538280838332E-5	0.0	0.0	14.507764253328206	0.0
10	7.495538280838332E-5	0.0	0.0	17.8337594546846	0.0
11	7.495538280838332E-5	0.0	0.0	20.353234737022788	0.0
12	7.495538280838332E-5	0.0	0.0	24.40389857937063	0.0
13	7.495538280838332E-5	0.0	0.0	25.226983637989488	0.0
14	7.495538280838332E-5	0.0	0.0	25.560909868400834	0.0
15	7.495538280838332E-5	0.0	0.0	26.363831929044235	0.0
16	7.495538280838332E-5	0.0	0.0	27.50367843541132	0.0
17	7.495538280838332E-5	0.0	0.0	28.99671470557151	0.0
18	7.495538280838332E-5	0.0	0.0	30.551888988079845	0.0
19	7.495538280838332E-5	0.0	0.0	32.04559985668531	0.0
20	7.495538280838332E-5	0.0	0.0	33.02204362853012	0.0
21	7.495538280838332E-5	0.0	0.0	34.04218638855222	0.0
22	1.4991076561676663E-4	0.0	0.0	35.189828254731374	0.0
23	1.4991076561676663E-4	0.0	0.0	36.183211943090875	0.0
24	1.4991076561676663E-4	0.0	0.0	37.00367356331144	0.0
25	1.4991076561676663E-4	0.0	0.0	37.69858491732796	0.0
26	2.2486614842514994E-4	0.0	0.0	38.31951530851261	0.0
27	2.2486614842514994E-4	0.0	0.0	38.96240762686011	0.0
28	7.495538280838331E-4	0.0	0.0	39.59495610238006	0.0
29	7.495538280838331E-4	0.0	0.0	40.27457655830367	0.0
30	7.495538280838331E-4	0.0	0.0	41.04841593041742	0.0
31	7.495538280838331E-4	0.0	0.0	41.73215893239549	0.0
32	7.495538280838331E-4	0.0	0.0	42.374526563063334	0.0
33	7.495538280838331E-4	0.0	0.0	42.97551882242095	0.0
34	7.495538280838331E-4	0.0	0.0	43.596674079754024	0.0
35	7.495538280838331E-4	0.0	0.0	44.272846588068454	0.0
36	7.495538280838331E-4	0.0	0.0	44.885531887144175	0.0
37	7.495538280838331E-4	0.0	0.0	45.50653723371163	0.0
38	7.495538280838331E-4	0.0	0.0	46.09298814880442	0.0
39	8.245092108922164E-4	0.0	0.0	46.681387903850236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGC	20	7.0336944E-4	45.000004	42
GGTACGA	25	3.891143E-5	45.000004	8
GTCGCAC	20	7.0336944E-4	45.000004	25
TACCCGT	25	3.891143E-5	45.000004	18
TCGCAAT	20	7.0336944E-4	45.000004	30
TGCCCGT	25	3.891143E-5	45.000004	42
GTCGACG	25	3.891143E-5	45.000004	1
CAACGTT	25	3.891143E-5	45.000004	43
ACGCATA	20	7.0336944E-4	45.000004	10
CGACGAT	20	7.0336944E-4	45.000004	31
TCCGATC	25	3.891143E-5	45.000004	34
CGCATTC	20	7.0336944E-4	45.000004	23
CGCGCGA	20	7.0336944E-4	45.000004	45
CGTCTCG	25	3.891143E-5	45.000004	41
GTCTACG	20	7.0336944E-4	45.000004	1
GCGCATC	20	7.0336944E-4	45.000004	43
CGTTACC	20	7.0336944E-4	45.000004	15
ATCCGGC	460	0.0	45.000004	6
CGCCTCG	20	7.0336944E-4	45.000004	40
TCGGATA	20	7.0336944E-4	45.000004	11
>>END_MODULE