SRR2934995.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934995.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821995
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	10685	1.2998862523494668	RNA PCR Primer, Index 41 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	8531	1.0378408627789706	Illumina PCR Primer Index 11 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	8171	0.9940449759426759	RNA PCR Primer, Index 41 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	3713	0.45170591061989435	Illumina PCR Primer Index 11 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	3008	0.365938965565484	Illumina PCR Primer Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1896	0.23065833733781835	RNA PCR Primer, Index 41 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1848	0.22481888575964573	RNA PCR Primer, Index 41 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1722	0.20949032536694262	Illumina PCR Primer Index 11 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1699	0.20669225481906828	RNA PCR Primer, Index 41 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1607	0.19549997262757074	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	1596	0.19416176497423948	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1464	0.1781032731342648	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	1416	0.17226382155609218	Illumina PCR Primer Index 11 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	1360	0.16545112804822415	RNA PCR Primer, Index 41 (95% over 23bp)
AGCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1072	0.13041441857918845	RNA PCR Primer, Index 41 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	983	0.11958710211132673	RNA PCR Primer, Index 41 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	972	0.11824889445799548	Illumina PCR Primer Index 11 (95% over 24bp)
GAAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	903	0.10985468281437234	RNA PCR Primer, Index 41 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGTA	35	1.211356E-7	45.000004	34
ACGTTAG	35	1.211356E-7	45.000004	1
TCCCTAT	35	1.211356E-7	45.000004	31
ATTCGAC	35	1.211356E-7	45.000004	13
TGCGCGA	35	1.211356E-7	45.000004	36
GTAGACG	35	1.211356E-7	45.000004	1
CTTGCAC	35	1.211356E-7	45.000004	21
AGTCTAT	35	1.211356E-7	45.000004	21
TCATCCG	35	1.211356E-7	45.000004	41
TTAGTAC	35	1.211356E-7	45.000004	35
GATCTTA	35	1.211356E-7	45.000004	29
ACTCCCC	35	1.211356E-7	45.000004	12
AGGGCCG	70	0.0	45.000004	6
ACGAATG	35	1.211356E-7	45.000004	1
AACGTTA	20	7.032113E-4	45.0	19
CGGAATT	25	3.8898324E-5	45.0	30
AGGTAAT	20	7.032113E-4	45.0	6
ACTATAT	20	7.032113E-4	45.0	37
TTGGCGA	25	3.8898324E-5	45.0	24
TCCGCTA	20	7.032113E-4	45.0	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content