SRR2934994.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934994.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1189160
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	16990	1.4287396145178108	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	15496	1.3031047125702178	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	14788	1.2435668875508763	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	8737	0.7347203067711662	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	6198	0.5212082478388106	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	4724	0.39725520535504055	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTAAATT	4308	0.3622725285075179	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	3601	0.3028187964613677	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2806	0.2359648827743954	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTC	2788	0.23445120925695448	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTA	2742	0.23058293249016115	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	2496	0.20989606108513573	No Hit
TGTTTTCTAATTGGTTATAACATTTTTTCAATTAATAGTTTCAAAACAAAT	2115	0.1778566382993037	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2019	0.16978371287295232	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	1929	0.16221534528574794	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	1431	0.12033704463655016	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1322	0.11117091055871371	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1264	0.10629351811362643	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1224	0.10292979918598003	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	1223	0.10284570621278885	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1205	0.10133203269534798	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.033385E-4	45.0	1
TATCACG	25	3.8908867E-5	45.0	11
TTAGGCG	25	3.8908867E-5	45.0	1
TCTTGCG	25	3.8908867E-5	45.0	16
GCGACTC	20	7.033385E-4	45.0	9
CTACTTA	20	7.033385E-4	45.0	43
CACGGTA	20	7.033385E-4	45.0	16
CGACGGA	35	1.2119744E-7	45.0	42
CGACGAA	25	3.8908867E-5	45.0	19
CTATCCG	25	3.8908867E-5	45.0	1
GCGAACC	35	1.2119744E-7	45.0	33
CGTTGTC	20	7.033385E-4	45.0	41
CCGTGAC	25	3.8908867E-5	45.0	43
TAAGTCG	25	3.8908867E-5	45.0	45
GCGCATT	25	3.8908867E-5	45.0	1
CCTCGCG	25	3.8908867E-5	45.0	21
CCCGTGC	20	7.033385E-4	45.0	12
ATTCACG	20	7.033385E-4	45.0	22
CTCGGTA	20	7.033385E-4	45.0	38
CGTAGTT	50	2.1827873E-11	45.0	29

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content