FastQCFastQC Report
Sat 14 Jan 2017
SRR2934993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4572491
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137250.300164614867476No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC76550.167414216889656RNA PCR Primer, Index 27 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC62660.13703690176754857No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC59160.12938243071446176RNA PCR Primer, Index 38 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC51530.1126956838187325No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG47980.10493186317917301No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT46890.10254804219406884RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG750.042.01
CGTTTTT80900.040.7447471
CGCGAAA451.9304935E-840.029
GTTAGCG3650.037.602741
AGTACGG8100.037.52
TACGGGA36900.036.7682884
CGTTAGG5000.036.4499972
CTATCGC250.002107763236.00000428
CGCGTAC250.002107763236.00000433
TTAATCG2550.035.29411720
CGTAAGG7450.035.0335542
TAGATCG1100.034.7727281
TGAACGG7200.034.68752
CGGGTAT3700.034.6621636
AACGGGA24300.034.6296274
TCGATTG653.6743586E-1034.6153831
TTACGAG1300.034.6153831
ATTACGG3650.034.520552
GTTTACG2350.034.4680861
TAAGGGA50550.034.451044
Produced by FastQC (version 0.11.5)