##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934992.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1967120
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1954303753711	33.0	31.0	34.0	30.0	34.0
2	32.372093212412054	34.0	31.0	34.0	30.0	34.0
3	32.37764498352922	34.0	31.0	34.0	30.0	34.0
4	35.92259292773191	37.0	35.0	37.0	35.0	37.0
5	35.84510350156574	37.0	35.0	37.0	35.0	37.0
6	35.793526068567246	37.0	35.0	37.0	35.0	37.0
7	36.131343791939486	37.0	35.0	37.0	35.0	37.0
8	35.93630739354996	37.0	35.0	37.0	35.0	37.0
9	37.652616007157675	39.0	38.0	39.0	35.0	39.0
10	37.37462737402904	39.0	37.0	39.0	34.0	39.0
11	37.285295253975356	39.0	37.0	39.0	34.0	39.0
12	37.268004493879374	39.0	37.0	39.0	34.0	39.0
13	37.23569024767173	39.0	37.0	39.0	34.0	39.0
14	38.31547287404937	40.0	38.0	41.0	34.0	41.0
15	38.37304129895482	40.0	38.0	41.0	34.0	41.0
16	38.43295477652609	40.0	38.0	41.0	34.0	41.0
17	38.471673309203304	40.0	38.0	41.0	34.0	41.0
18	38.48771961039489	40.0	38.0	41.0	34.0	41.0
19	38.485009557119035	40.0	38.0	41.0	34.0	41.0
20	38.45588271178169	40.0	38.0	41.0	34.0	41.0
21	38.41175627313026	40.0	38.0	41.0	34.0	41.0
22	38.36544796453699	40.0	37.0	41.0	34.0	41.0
23	38.11707165805848	40.0	37.0	41.0	34.0	41.0
24	37.908144902192035	40.0	37.0	41.0	33.0	41.0
25	37.875216051893126	40.0	37.0	41.0	33.0	41.0
26	38.080038838505025	40.0	37.0	41.0	34.0	41.0
27	38.11578144698849	40.0	37.0	41.0	34.0	41.0
28	38.12651693846842	40.0	37.0	41.0	34.0	41.0
29	38.18657580625483	40.0	37.0	41.0	34.0	41.0
30	38.11069736467526	40.0	37.0	41.0	34.0	41.0
31	38.058569888974745	40.0	37.0	41.0	34.0	41.0
32	38.00098214648827	40.0	37.0	41.0	34.0	41.0
33	37.94152009028427	40.0	37.0	41.0	34.0	41.0
34	37.853450221643826	40.0	37.0	41.0	34.0	41.0
35	37.81113760217983	40.0	37.0	41.0	33.0	41.0
36	37.7509211436008	40.0	37.0	41.0	33.0	41.0
37	37.677693785839196	40.0	37.0	41.0	33.0	41.0
38	37.65188854772459	40.0	37.0	41.0	33.0	41.0
39	37.607765159217536	40.0	36.0	41.0	33.0	41.0
40	37.549078856399206	40.0	36.0	41.0	33.0	41.0
41	37.49946724144943	40.0	36.0	41.0	33.0	41.0
42	37.48922282321363	40.0	36.0	41.0	33.0	41.0
43	37.398981251779254	40.0	36.0	41.0	33.0	41.0
44	37.30035127496035	40.0	36.0	41.0	32.0	41.0
45	37.32056356500875	40.0	36.0	41.0	33.0	41.0
46	37.21099627882386	39.0	35.0	41.0	32.0	41.0
47	37.142873846028714	39.0	35.0	41.0	32.0	41.0
48	37.106188234576436	39.0	35.0	41.0	32.0	41.0
49	37.07630190329009	39.0	35.0	41.0	32.0	41.0
50	37.01095815201919	39.0	35.0	41.0	32.0	41.0
51	36.00283053397861	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	14.0
10	22.0
11	21.0
12	16.0
13	17.0
14	24.0
15	47.0
16	82.0
17	145.0
18	275.0
19	620.0
20	962.0
21	1608.0
22	2550.0
23	3715.0
24	5314.0
25	7154.0
26	9135.0
27	11368.0
28	14149.0
29	18016.0
30	23126.0
31	31151.0
32	41737.0
33	59270.0
34	103558.0
35	138721.0
36	137968.0
37	220325.0
38	433575.0
39	701856.0
40	572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86665785513848	23.06137907194274	26.73909065029078	10.332872422628006
2	30.482176989710847	24.293179877180854	26.778132498271585	18.446510634836716
3	30.948086542763026	24.919933710195615	27.419882874456057	16.712096872585303
4	28.858483468217493	24.831886209280572	27.272052543820408	19.037577778681523
5	25.381217210947987	30.111279433893205	24.973514579690107	19.533988775468707
6	26.030084590670626	32.704613851722314	24.825379234617106	16.439922322989954
7	81.53391760543333	4.916883565822116	8.276363414534954	5.272835414209606
8	81.17664402781732	3.6911830493310016	8.345449184594738	6.786723738256945
9	75.59792996868518	7.129559965838384	10.422953353125381	6.849556712351052
10	34.98530847126764	35.77422831347351	16.997387042986702	12.243076172272154
11	23.544572776444753	25.0441762576762	31.135721257472852	20.275529708406197
12	23.0797307739233	23.271534019276913	33.97393143275448	19.674803774045305
13	21.921895969742568	25.553906218227663	34.219773069258615	18.304424742771157
14	17.99483508886087	29.749176461019154	32.02229655537029	20.233691894749683
15	15.780074423522713	28.669730367237385	36.135975436170646	19.41421977306926
16	18.885172231485623	30.117633901338	31.00883525153524	19.98835861564114
17	19.321902070031317	28.56724551628777	28.990808898287852	23.120043515393064
18	19.926745699296433	27.793728903168084	32.441284720810124	19.838240676725366
19	19.870368864126238	28.94012566594819	29.517060474195777	21.672444995729798
20	21.089206555776975	31.166273536947415	29.643946480133394	18.10057342714222
21	20.11321119199642	29.361858961324174	31.79475578510716	18.730174061572246
22	19.36704420675912	26.84686241815446	29.434299890194804	24.35179348489162
23	17.295691162715034	28.21251372564968	31.03115214120135	23.460642970433934
24	19.88922892350238	27.103023709788932	30.717495628126397	22.290251738582292
25	17.575948594900158	29.74617715238521	28.559976005530928	24.1178982471837
26	17.53914351945992	30.213052584489	28.08517019805604	24.16263369799504
27	19.282046850217576	29.997712391719876	28.842876896173088	21.877363861889464
28	15.483295375981129	28.516867298385456	29.833004595550854	26.166832730082557
29	16.858097116596852	26.36875228760828	30.915856683883035	25.85729391191183
30	19.05415022977754	28.120246858351294	29.77922038309813	23.04638252877303
31	18.287801455935583	28.362580828825894	26.459494082719914	26.890123632518602
32	18.36644434503233	28.308949123591848	27.89479035341006	25.42981617796576
33	19.933964374313717	26.871416080361136	28.565008743747207	24.629610801577943
34	17.195087234129083	24.614410915449998	28.887002318109722	29.303499532311193
35	16.951126519988613	25.113414534954654	29.090345276343083	28.84511366871365
36	19.990442880963034	26.978069461954533	27.922292488511125	25.109195168571315
37	17.303164016430113	26.57956809955671	30.112753670340396	26.00451421367278
38	17.63659563219326	26.535137663182724	29.61924031070804	26.20902639391598
39	20.774431656431737	23.682439302126966	31.49609581520192	24.047033226239375
40	19.93198178047094	23.08186587498475	31.19367400056936	25.79247834397495
41	18.610557566391474	25.011082191223714	26.75591728008459	29.622442962300216
42	20.86247915734678	24.441264386514295	28.29085160030908	26.405404855829843
43	21.555929480662083	24.67348204481679	29.418744153889953	24.351844320631177
44	19.296535035991706	24.2764040831266	28.962493391353856	27.464567489527838
45	18.93544877790882	23.46481150107772	29.159685225100656	28.440054495912808
46	21.392441742242465	23.43166659888568	29.59295823335638	25.582933425515474
47	17.339104884297857	24.097513115620806	31.96983407214608	26.59354792793526
48	18.100522591402658	23.850553092846393	30.346293057871403	27.70263125787954
49	20.94361299768189	21.169171174102242	33.50253161983001	24.384684208385863
50	19.29597584285656	21.85850380251332	31.308562771971204	27.53695758265891
51	18.19446703810647	21.733397047460244	28.132244092887067	31.93989182154622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	812.0
1	1219.5
2	1627.0
3	2513.5
4	3400.0
5	2548.0
6	1696.0
7	1922.5
8	2149.0
9	2470.0
10	2791.0
11	3322.5
12	3854.0
13	4111.5
14	4369.0
15	4796.0
16	5223.0
17	5155.0
18	5087.0
19	5191.5
20	5296.0
21	6329.5
22	7363.0
23	8613.5
24	9864.0
25	11949.0
26	17895.5
27	21757.0
28	26333.5
29	30910.0
30	34549.0
31	38188.0
32	43704.5
33	49221.0
34	55147.5
35	61074.0
36	67443.5
37	73813.0
38	80693.0
39	87573.0
40	91020.5
41	94468.0
42	105682.5
43	116897.0
44	126426.0
45	135955.0
46	147407.5
47	158860.0
48	183502.5
49	208145.0
50	233960.5
51	259776.0
52	244096.0
53	228416.0
54	179278.5
55	130141.0
56	103716.5
57	77292.0
58	63819.0
59	50346.0
60	39873.0
61	29400.0
62	24812.0
63	20224.0
64	15945.0
65	11666.0
66	9187.5
67	6709.0
68	5105.0
69	3501.0
70	2806.0
71	2111.0
72	1653.0
73	1195.0
74	1011.5
75	737.5
76	647.0
77	467.0
78	287.0
79	173.5
80	60.0
81	66.0
82	72.0
83	42.5
84	13.0
85	8.0
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1967120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.11614798078539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.38354532254766	12.549166292744008
2	13.548167089707578	5.450738680899283
3	6.554969632800765	3.95582217428924
4	3.887755447630934	3.1282665559059355
5	2.452103314453617	2.4663436568861643
6	1.756756584042946	2.120350524649616
7	1.2200856535136806	1.718039648872011
8	0.9475092496683444	1.5248189023593073
9	0.7160783484182442	1.2964264220356019
>10	4.481579150835975	18.059383702168212
>50	1.1389704277287434	16.82055113833435
>100	0.892253657490887	25.447660189105026
>500	0.01408147675736398	1.9191466022400854
>1k	0.005120537002677811	1.7362603723456982
>5k	7.680805504016716E-4	1.1466570043279731
>10k+	2.5602685013389055E-4	0.6603681328374628
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	12822	0.6518158526170239	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	9731	0.4946825816421977	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	6819	0.3466489080483143	No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	5714	0.29047541583634956	No Hit
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	3734	0.18982065151083818	No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	2531	0.12866525682215627	No Hit
ACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	2477	0.12592012688600593	No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	2253	0.11453292122493798	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC	2216	0.11265199886127944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0501443735003454E-4	0.0	0.0	0.3639838952377079	0.0
2	3.0501443735003454E-4	0.0	0.0	2.0803001342063525	0.0
3	3.0501443735003454E-4	0.0	0.0	2.9756191793078206	0.0
4	3.0501443735003454E-4	0.0	0.0	4.0687909227703445	0.0
5	3.0501443735003454E-4	0.0	0.0	6.870450201309529	0.0
6	3.0501443735003454E-4	0.0	0.0	8.172709341575501	0.0
7	3.5585017690837364E-4	0.0	0.0	9.631085038025134	0.0
8	3.5585017690837364E-4	0.0	0.0	11.82327463499939	0.0
9	3.5585017690837364E-4	0.0	0.0	12.575389401765017	0.0
10	3.5585017690837364E-4	0.0	0.0	14.314785066493148	0.0
11	3.5585017690837364E-4	0.0	0.0	17.096821749562814	0.0
12	3.5585017690837364E-4	0.0	0.0	19.206454105494327	0.0
13	3.5585017690837364E-4	0.0	0.0	19.97031192809793	0.0
14	3.5585017690837364E-4	0.0	0.0	20.24634999389971	0.0
15	3.5585017690837364E-4	0.0	0.0	20.75419903208752	0.0
16	3.5585017690837364E-4	0.0	0.0	21.84259221603156	0.0
17	3.5585017690837364E-4	0.0	0.0	23.341483996909187	0.0
18	3.5585017690837364E-4	0.0	0.0	24.8482044816788	0.0
19	3.5585017690837364E-4	0.0	0.0	25.820438000732036	0.0
20	3.5585017690837364E-4	0.0	0.0	26.684543901744682	0.0
21	3.5585017690837364E-4	0.0	0.0	27.742486477693276	0.0
22	3.5585017690837364E-4	0.0	0.0	28.905150677132053	0.0
23	3.5585017690837364E-4	0.0	0.0	29.94982512505592	0.0
24	3.5585017690837364E-4	0.0	0.0	30.745404449143926	0.0
25	3.5585017690837364E-4	0.0	0.0	31.406014884704543	0.0
26	3.5585017690837364E-4	0.0	0.0	32.04781609662857	0.0
27	3.5585017690837364E-4	0.0	0.0	32.70019114238074	0.0
28	3.5585017690837364E-4	0.0	0.0	33.34407661962666	0.0
29	3.5585017690837364E-4	0.0	0.0	34.049727520435965	0.0
30	3.5585017690837364E-4	0.0	0.0	34.83442799625849	0.0
31	3.5585017690837364E-4	0.0	0.0	35.51496604172598	0.0
32	3.5585017690837364E-4	0.0	0.0	36.14858269958111	0.0
33	4.0668591646671274E-4	0.0	0.0	36.755256415470335	0.0
34	4.0668591646671274E-4	0.0	0.0	37.40000610028875	0.0
35	4.0668591646671274E-4	0.0	0.0	38.07205457724999	0.0
36	4.0668591646671274E-4	0.0	0.0	38.69138639228923	0.0
37	4.5752165602505184E-4	0.0	0.0	39.31986864044898	0.0
38	4.5752165602505184E-4	0.0	0.0	39.970515271056165	0.0
39	4.5752165602505184E-4	0.0	0.0	40.68760421326609	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAG	35	1.2125201E-7	45.000004	2
ACACGTT	35	1.2125201E-7	45.000004	42
TCCGCTA	25	3.891821E-5	45.000004	45
TAACGCG	35	1.2125201E-7	45.000004	1
CGCTAAG	25	3.891821E-5	45.000004	2
TTGCGTA	25	3.891821E-5	45.000004	13
TTACGGA	50	2.1827873E-11	45.000004	36
ACCGTAC	25	3.891821E-5	45.000004	44
ACGAGTC	35	1.2125201E-7	45.000004	36
TCGCGTG	25	3.891821E-5	45.000004	1
GTACGTT	25	3.891821E-5	45.000004	29
TGCCCCG	40	6.8193913E-9	45.0	33
GTATTCG	20	7.0345134E-4	45.0	33
CGAGTTA	20	7.0345134E-4	45.0	38
TACTTCG	20	7.0345134E-4	45.0	31
CGACCGG	165	0.0	45.0	2
CCTACGC	20	7.0345134E-4	45.0	28
CAATTCG	20	7.0345134E-4	45.0	23
TCGATTA	20	7.0345134E-4	45.0	41
CGTTCCG	20	7.0345134E-4	45.0	19
>>END_MODULE