##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934991.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867656
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.161523691416875	33.0	31.0	34.0	30.0	34.0
2	32.3509973998912	34.0	31.0	34.0	30.0	34.0
3	32.366891947960944	34.0	31.0	34.0	30.0	34.0
4	35.92129138737011	37.0	35.0	37.0	35.0	37.0
5	35.815648137049706	37.0	35.0	37.0	35.0	37.0
6	35.751913200623285	37.0	35.0	37.0	35.0	37.0
7	36.02668684363388	37.0	35.0	37.0	35.0	37.0
8	35.797267580700186	37.0	35.0	37.0	35.0	37.0
9	37.51886692421881	39.0	37.0	39.0	35.0	39.0
10	37.34958555003365	39.0	37.0	39.0	34.0	39.0
11	37.27653009948643	39.0	37.0	39.0	34.0	39.0
12	37.07724144130854	39.0	37.0	39.0	34.0	39.0
13	36.90716597361166	39.0	35.0	39.0	33.0	39.0
14	37.98977935956185	40.0	37.0	41.0	33.0	41.0
15	38.1002839835142	40.0	37.0	41.0	33.0	41.0
16	38.16838816305079	40.0	37.0	41.0	34.0	41.0
17	38.20616465511677	40.0	37.0	41.0	34.0	41.0
18	38.23389914897148	40.0	37.0	41.0	34.0	41.0
19	38.203133499912404	40.0	36.0	41.0	34.0	41.0
20	38.164171053966086	40.0	36.0	41.0	34.0	41.0
21	38.087879297786216	40.0	36.0	41.0	34.0	41.0
22	38.016092783315045	40.0	36.0	41.0	34.0	41.0
23	37.81221013858027	40.0	36.0	41.0	33.0	41.0
24	37.59023737518095	39.0	35.0	41.0	33.0	41.0
25	37.55699609061656	39.0	35.0	41.0	33.0	41.0
26	37.68273140507298	39.0	35.0	41.0	33.0	41.0
27	37.755653162082666	39.0	36.0	41.0	34.0	41.0
28	37.73542279428714	40.0	36.0	41.0	33.0	41.0
29	37.76914814165983	40.0	36.0	41.0	34.0	41.0
30	37.71290234839614	40.0	36.0	41.0	33.0	41.0
31	37.58051923803904	39.0	35.0	41.0	33.0	41.0
32	37.46053735581844	39.0	35.0	41.0	33.0	41.0
33	37.406438726868714	39.0	35.0	41.0	33.0	41.0
34	37.28437076445043	39.0	35.0	41.0	33.0	41.0
35	37.262589090607335	39.0	35.0	41.0	33.0	41.0
36	37.20249154042616	39.0	35.0	41.0	33.0	41.0
37	37.06984795817697	39.0	35.0	41.0	32.0	41.0
38	36.99544519947998	39.0	35.0	41.0	32.0	41.0
39	36.947823791917536	39.0	35.0	41.0	32.0	41.0
40	36.90143098186378	39.0	35.0	41.0	32.0	41.0
41	36.90553399042939	39.0	35.0	41.0	32.0	41.0
42	36.894818914408475	39.0	35.0	41.0	32.0	41.0
43	36.766201121181666	39.0	35.0	41.0	31.0	41.0
44	36.621721050739005	39.0	35.0	41.0	31.0	41.0
45	36.65218358427764	39.0	35.0	41.0	31.0	41.0
46	36.54359907613156	39.0	35.0	40.0	31.0	41.0
47	36.47391362475451	39.0	35.0	40.0	31.0	41.0
48	36.45556649178937	39.0	35.0	40.0	31.0	41.0
49	36.4013410844851	39.0	35.0	40.0	31.0	41.0
50	36.330076666328594	38.0	35.0	40.0	31.0	41.0
51	35.32762869155518	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	15.0
10	11.0
11	13.0
12	11.0
13	22.0
14	17.0
15	28.0
16	49.0
17	86.0
18	187.0
19	316.0
20	620.0
21	1039.0
22	1744.0
23	2548.0
24	3464.0
25	4192.0
26	5034.0
27	5809.0
28	7031.0
29	8562.0
30	11038.0
31	14588.0
32	19971.0
33	29131.0
34	57560.0
35	85143.0
36	65054.0
37	101594.0
38	188795.0
39	253819.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.888273693721935	23.41204348267055	25.363277612325625	11.33640521128189
2	30.15238758217542	23.989230755045778	25.96247821717363	19.89590344560517
3	30.254617037166803	24.78516831555363	26.930373327678254	18.029841319601317
4	28.02447052749016	26.019989488921873	27.0788192555575	18.876720728030463
5	23.80482587569267	29.160519837354897	25.989447430779016	21.045206856173415
6	25.531777570834524	33.261453848068825	25.06557898521995	16.141189595876707
7	76.81558129028095	6.993209290317822	9.670768138524945	6.520441280876292
8	77.1642217653079	4.77551010999751	10.120485538047337	7.939782586647243
9	71.24666918686668	7.711581548447772	12.413329706704038	8.628419557981504
10	39.11515623703403	25.68552513899518	19.55406290050435	15.645255723466441
11	29.953691324672448	24.348244004536358	24.709216555870068	20.988848114921122
12	28.725670081230348	20.64285846003485	31.048941054980318	19.582530403754483
13	22.939390726278617	26.407931253860976	31.138031662317783	19.51464635754262
14	17.391108918741992	31.13249951593719	28.530200909116054	22.946190656204763
15	15.77134255972413	26.66702010935209	36.645513890297536	20.91612344062624
16	17.594876310427175	25.826825377799494	32.80608904911624	23.77220926265709
17	17.52745327641369	25.63227823008197	28.164041970550542	28.676226522953797
18	19.130046931041793	24.86296412403072	32.59897931899278	23.408009625934703
19	20.049881519865014	27.3116304157408	28.604193366956487	24.034294697437694
20	23.144886913707737	26.825377799496575	29.62141678268807	20.40831850410762
21	21.105714707211153	28.432466323058907	29.288681228505308	21.173137741224632
22	18.68770572669353	27.047124666918688	27.364185806356435	26.900983800031348
23	18.518744755986244	26.901099053080944	28.654443696580213	25.9257124943526
24	20.988271849673144	24.527923508856045	29.950003227085386	24.533801414385426
25	18.48589763685147	27.00494205076666	28.25128852909448	26.257871783287385
26	17.011580626423374	28.93911872908157	30.245742552347938	23.803558092147117
27	19.309611182311883	27.66856911033866	30.1075541458827	22.914265561466756
28	15.725702352084237	25.53442839097523	31.32116875812534	27.418700498815195
29	17.272167771559236	25.378952027070632	32.28756557898522	25.061314622384906
30	19.45068091501701	25.178065961625347	29.89156993094037	25.479683192417273
31	19.761864148925383	27.30333219616991	24.896618014512665	28.038185640392044
32	20.04123754114534	27.84559779451764	25.800778188590872	26.31238647574615
33	21.635763482301744	24.592119457480845	25.657864407092212	28.1142526531252
34	17.5968356122703	23.39417925998322	28.34279945047346	30.666185677273024
35	17.89222917838406	23.17427644135464	28.800930322616335	30.132564057644966
36	21.53145947241764	23.13197857215302	29.407737628737657	25.928824326691686
37	18.645523110541504	25.463547765473876	29.58130872142877	26.30962040255585
38	18.283513281761437	27.07248033782974	24.826083148160098	29.817923232248727
39	21.08174207289525	23.74535530210129	27.34666734281789	27.826235282185568
40	20.826341314991197	21.130263606775035	28.91480033561688	29.128594742616894
41	18.777372599278976	21.66918686668449	27.782669629438395	31.770770904598134
42	22.110490793586397	20.28061812515559	29.486685967710706	28.122205113547306
43	22.66658675788561	22.331776648810127	28.61433563532091	26.38730095798335
44	18.71951556838194	23.193754206736312	27.498801368284205	30.587928856597546
45	19.17107701669786	23.17957808163604	26.364135095014614	31.28520980665148
46	21.349474907106043	22.22954719381875	28.985911467217424	27.435066431857784
47	17.422457748232016	22.60596365379828	31.807767133518354	28.16381146445135
48	18.62581483906064	20.19060549342136	30.983362069760368	30.20021759775764
49	20.842130982785807	18.973302783591652	32.80055690273564	27.384009330886894
50	19.47903316521755	19.361936066828328	30.994887374719937	30.164143393234184
51	17.9580386697032	19.984648293793853	27.1297611034788	34.92755193302415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1242.0
1	1287.5
2	1333.0
3	1545.0
4	1757.0
5	1446.0
6	1135.0
7	1211.5
8	1288.0
9	1374.5
10	1461.0
11	1572.0
12	1683.0
13	1763.5
14	1844.0
15	2057.0
16	2270.0
17	2079.0
18	1888.0
19	2200.0
20	2512.0
21	2245.0
22	1978.0
23	2447.5
24	2917.0
25	3129.0
26	4509.0
27	5677.0
28	5830.0
29	5983.0
30	7794.5
31	9606.0
32	11549.0
33	13492.0
34	15215.0
35	16938.0
36	18500.5
37	20063.0
38	23136.5
39	26210.0
40	30104.5
41	33999.0
42	39921.5
43	45844.0
44	51560.5
45	57277.0
46	62429.0
47	67581.0
48	80373.0
49	93165.0
50	107686.0
51	122207.0
52	116519.0
53	110831.0
54	88957.5
55	67084.0
56	54657.0
57	42230.0
58	36522.5
59	30815.0
60	25922.5
61	21030.0
62	18994.5
63	16959.0
64	14448.5
65	11938.0
66	9454.0
67	6970.0
68	6089.0
69	5208.0
70	4335.0
71	3462.0
72	3249.0
73	3036.0
74	2393.0
75	1288.5
76	827.0
77	585.0
78	343.0
79	309.5
80	276.0
81	182.0
82	88.0
83	88.5
84	89.0
85	51.0
86	13.0
87	11.0
88	9.0
89	5.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	867656.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.343809699197955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.68699226570739	13.379792335343527
2	12.95327794323376	5.529445988023992
3	6.519106391134802	4.174276986636191
4	3.831642954156373	3.271274321951452
5	2.627072613732459	2.803586896674009
6	1.7660551888553093	2.261660752152529
7	1.36767163951401	2.043392624334397
8	0.9513858307167796	1.6244958497065844
9	0.750227427272404	1.4411440294938958
>10	4.631066486075382	19.509505816529575
>50	1.2471252138120377	19.70450045233686
>100	0.6508878745176334	19.25378393129982
>500	0.012023117749276184	1.6492445369625426
>1k	0.004372042817918612	1.8690038344826947
>5k	0.001093010704479653	1.4848916440719406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	6506	0.7498363406695742	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	6224	0.7173349806835889	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	4883	0.5627806411757654	No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	2815	0.32443733461187385	No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	2184	0.251712660316992	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG	1541	0.17760494942696184	No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG	1289	0.14856118092884738	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG	1229	0.14164599795310584	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCC	1080	0.12447329356334769	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCC	1002	0.11548355569488368	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	949	0.10937514406631199	No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG	931	0.10730058917358953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.152530495956923E-4	0.0	0.0	0.5864075163428825	0.0
2	1.152530495956923E-4	0.0	0.0	2.7233143088966134	0.0
3	1.152530495956923E-4	0.0	0.0	3.7563273924228033	0.0
4	1.152530495956923E-4	0.0	0.0	4.90770535788377	0.0
5	1.152530495956923E-4	0.0	0.0	7.843431037185244	0.0
6	1.152530495956923E-4	0.0	0.0	9.294121172446223	0.0
7	2.305060991913846E-4	0.0	0.0	10.807278460588067	0.0
8	2.305060991913846E-4	0.0	0.0	13.041343573950966	0.0
9	2.305060991913846E-4	0.0	0.0	13.873816351180652	0.0
10	2.305060991913846E-4	0.0	0.0	15.80211512396618	0.0
11	2.305060991913846E-4	0.0	0.0	18.897581530007283	0.0
12	2.305060991913846E-4	0.0	0.0	21.10260287487207	0.0
13	2.305060991913846E-4	0.0	0.0	21.97161086882359	0.0
14	2.305060991913846E-4	0.0	0.0	22.33431221590123	0.0
15	2.305060991913846E-4	0.0	0.0	22.9612888057018	0.0
16	3.457591487870769E-4	0.0	0.0	24.38028435232396	0.0
17	3.457591487870769E-4	0.0	0.0	26.010423485805436	0.0
18	3.457591487870769E-4	0.0	0.0	27.89031597776077	0.0
19	4.610121983827692E-4	0.0	0.0	28.94741694865246	0.0
20	4.610121983827692E-4	0.0	0.0	29.90505453774307	0.0
21	4.610121983827692E-4	0.0	0.0	31.062886673981392	0.0
22	4.610121983827692E-4	0.0	0.0	32.18210903860516	0.0
23	4.610121983827692E-4	0.0	0.0	33.293955208054804	0.0
24	4.610121983827692E-4	0.0	0.0	34.20583733645592	0.0
25	4.610121983827692E-4	0.0	0.0	34.979992070590185	0.0
26	4.610121983827692E-4	0.0	0.0	35.69824907567054	0.0
27	4.610121983827692E-4	0.0	0.0	36.34274412900965	0.0
28	4.610121983827692E-4	0.0	0.0	37.02780825580645	0.0
29	4.610121983827692E-4	0.0	0.0	37.74410595904368	0.0
30	4.610121983827692E-4	0.0	0.0	38.58487695584425	0.0
31	4.610121983827692E-4	0.0	0.0	39.29760181454401	0.0
32	4.610121983827692E-4	0.0	0.0	39.96572374305024	0.0
33	4.610121983827692E-4	0.0	0.0	40.578063195552154	0.0
34	4.610121983827692E-4	0.0	0.0	41.236964880090724	0.0
35	4.610121983827692E-4	0.0	0.0	41.926869634970544	0.0
36	4.610121983827692E-4	0.0	0.0	42.5647952644827	0.0
37	4.610121983827692E-4	0.0	0.0	43.192809131729625	0.0
38	4.610121983827692E-4	0.0	0.0	43.786938602395416	0.0
39	4.610121983827692E-4	0.0	0.0	44.43984712835502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	60	0.0	45.000004	25
TACTTAT	30	2.164883E-6	45.000004	36
GGCCGTA	30	2.164883E-6	45.000004	8
CTAACGA	30	2.164883E-6	45.000004	10
GGTATTC	30	2.164883E-6	45.000004	8
ACTCGGT	30	2.164883E-6	45.000004	28
TGCTCTA	45	3.8380676E-10	45.000004	23
CATCGTT	30	2.164883E-6	45.000004	36
ACGTAAC	30	2.164883E-6	45.000004	22
CGTCCAT	30	2.164883E-6	45.000004	35
CCACGTA	30	2.164883E-6	45.000004	20
CGTCATT	30	2.164883E-6	45.000004	6
CAATAGT	30	2.164883E-6	45.000004	25
AATACGG	75	0.0	45.000004	2
TGCGTAG	60	0.0	45.000004	1
GCATAAC	35	1.2114651E-7	45.0	42
AGCGTTA	25	3.890012E-5	45.0	1
AATCCGA	20	7.0323306E-4	45.0	25
GTACCGA	20	7.0323306E-4	45.0	24
TCTTGCG	25	3.890012E-5	45.0	36
>>END_MODULE