SRR2934990.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934990.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1352602
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	36035	2.664124406144601	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	31453	2.325369916649539	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	30948	2.288034469858835	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	15813	1.1690800398047616	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	8385	0.6199162798812954	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT	8255	0.6103051747668568	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT	8120	0.6003244117634012	No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	6737	0.49807703966133426	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC	5870	0.4339783617058085	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA	5397	0.39900872540481236	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	5004	0.3699536153280862	No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	4095	0.3027498111048187	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	3644	0.2694066695154968	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	3027	0.22379088601081473	No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2852	0.21085285989522418	TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1996	0.14756742929553557	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATG	1754	0.12967598746711895	No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1674	0.12376146124284895	No Hit
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1638	0.12109992444192749	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1635	0.12087812970851737	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTAATGAC	1577	0.11659009819592163	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC	1467	0.10845762463755045	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT	1466	0.10838369305974707	No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1465	0.1083097614819437	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTAT	1445	0.1068311299258762	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTAATGACTCGT	1422	0.10513070363639858	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	40	6.8157533E-9	45.000004	25
CTTAACG	20	7.033729E-4	45.000004	1
AACGTAA	25	3.8911723E-5	45.000004	10
CGAACTT	20	7.033729E-4	45.000004	45
ACGTTCA	40	6.8157533E-9	45.000004	26
GACGTAA	20	7.033729E-4	45.000004	1
CGGCACG	25	3.8911723E-5	45.000004	2
CCGGATT	20	7.033729E-4	45.000004	44
ATAGCGC	25	3.8911723E-5	45.000004	12
ACATTCG	25	3.8911723E-5	45.000004	14
GCCGGAT	20	7.033729E-4	45.000004	45
TTCCGTA	20	7.033729E-4	45.000004	38
CGCGATA	20	7.033729E-4	45.000004	11
ATATGCG	20	7.033729E-4	45.000004	2
TGCGGTA	20	7.033729E-4	45.000004	18
TTATCCG	20	7.033729E-4	45.000004	1
CCGTCCG	20	7.033729E-4	45.000004	23
TCGGCCC	25	3.8911723E-5	45.000004	14
TCGGATC	25	3.8911723E-5	45.000004	29
CTAACGG	40	6.8157533E-9	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content