##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934990.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1352602
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02069049136405	33.0	31.0	34.0	30.0	34.0
2	32.193628280898594	33.0	31.0	34.0	30.0	34.0
3	32.23150860341771	34.0	31.0	34.0	30.0	34.0
4	35.806481877152336	37.0	35.0	37.0	35.0	37.0
5	35.7178578768921	37.0	35.0	37.0	35.0	37.0
6	35.61295192525222	37.0	35.0	37.0	33.0	37.0
7	35.9115061193167	37.0	35.0	37.0	35.0	37.0
8	35.34490190018941	37.0	35.0	37.0	33.0	37.0
9	37.285162967376955	39.0	37.0	39.0	34.0	39.0
10	37.217308565269015	39.0	37.0	39.0	34.0	39.0
11	37.15801100397604	39.0	37.0	39.0	34.0	39.0
12	36.98806744334254	39.0	35.0	39.0	33.0	39.0
13	36.79937187731498	39.0	35.0	39.0	33.0	39.0
14	37.820889663034656	40.0	36.0	41.0	33.0	41.0
15	37.77312616719478	40.0	36.0	41.0	33.0	41.0
16	37.98974421152712	40.0	36.0	41.0	33.0	41.0
17	37.90232159940618	40.0	36.0	41.0	33.0	41.0
18	37.93086066706984	40.0	36.0	41.0	33.0	41.0
19	37.942819099779534	40.0	36.0	41.0	34.0	41.0
20	37.78647894946185	40.0	36.0	41.0	33.0	41.0
21	37.86208359887092	40.0	36.0	41.0	33.0	41.0
22	37.80823479486205	40.0	36.0	41.0	33.0	41.0
23	37.51304005169296	39.0	35.0	41.0	33.0	41.0
24	37.254537550587685	39.0	35.0	41.0	32.0	41.0
25	37.29359338519387	39.0	35.0	41.0	32.0	41.0
26	37.50908101570159	39.0	35.0	41.0	33.0	41.0
27	37.514448448250114	39.0	36.0	41.0	33.0	41.0
28	37.47521887443609	39.0	36.0	41.0	33.0	41.0
29	37.552668856027125	39.0	36.0	41.0	33.0	41.0
30	37.4901249591528	39.0	36.0	41.0	33.0	41.0
31	37.383494923118555	39.0	36.0	41.0	33.0	41.0
32	37.16411405572371	39.0	35.0	41.0	32.0	41.0
33	37.162486082380475	39.0	35.0	41.0	32.0	41.0
34	37.029702750698284	39.0	35.0	41.0	32.0	41.0
35	36.954474413020236	39.0	35.0	41.0	32.0	41.0
36	37.00979445542739	39.0	35.0	41.0	32.0	41.0
37	36.84842178260863	39.0	35.0	41.0	31.0	41.0
38	36.70476829104201	39.0	35.0	41.0	31.0	41.0
39	36.688126292878465	39.0	35.0	41.0	31.0	41.0
40	36.54297864412444	39.0	35.0	40.0	30.0	41.0
41	36.462688211314195	39.0	35.0	40.0	30.0	41.0
42	36.55106454078879	39.0	35.0	40.0	31.0	41.0
43	36.56659386870639	39.0	35.0	40.0	31.0	41.0
44	36.473845965036276	39.0	35.0	40.0	31.0	41.0
45	36.47380086677382	39.0	35.0	40.0	31.0	41.0
46	36.31858151917563	38.0	35.0	40.0	30.0	41.0
47	36.04662421022592	38.0	35.0	40.0	30.0	41.0
48	36.01025504915711	38.0	35.0	40.0	30.0	41.0
49	36.08381401180835	38.0	35.0	40.0	30.0	41.0
50	36.01067645915059	38.0	35.0	40.0	30.0	41.0
51	34.876694696592196	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	18.0
11	15.0
12	16.0
13	14.0
14	28.0
15	43.0
16	69.0
17	151.0
18	345.0
19	669.0
20	1383.0
21	2532.0
22	4001.0
23	5290.0
24	6779.0
25	7676.0
26	8855.0
27	10227.0
28	12710.0
29	16025.0
30	20604.0
31	28213.0
32	37286.0
33	52930.0
34	92372.0
35	122358.0
36	106201.0
37	161557.0
38	290735.0
39	363236.0
40	255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.75018815586551	19.27462771753997	23.471723389437543	9.503460737156976
2	26.80064054319009	34.65712752162129	21.50203829360004	17.040193641588584
3	27.10457325953976	34.35038540531509	23.42536829015483	15.119673044990323
4	25.63185622969654	22.26382927128601	35.554065423531824	16.55024907548562
5	31.60530592147579	24.999297650010867	22.50432869388039	20.89106773463295
6	22.491316736186995	37.75279054740419	24.49345779468018	15.262434921728637
7	68.77485025158916	5.4179278161646955	19.33902212180671	6.468199810439435
8	65.86039352300233	12.106221933724777	12.684884393191789	9.348500150081103
9	61.03443585030925	6.7153530750361154	13.168322980448055	19.081888094206573
10	40.62185328721974	21.87228763523934	21.0345689271493	16.471290150391617
11	31.043573793325752	21.2929597915721	25.80988346904707	21.853582946055088
12	25.516079378856453	18.36430820004702	31.807804513079237	24.31180790801729
13	22.887072472168455	22.801237910338738	37.073359347391175	17.238330270101628
14	18.565476023250003	30.203932864212828	28.680055182529674	22.550535930007495
15	14.568513132466165	23.32371237067519	41.842759363064665	20.265015133793977
16	15.90807939068551	26.836793084735937	28.346475903480844	28.908651621097707
17	16.18088691277996	26.456193322204168	31.24244973761683	26.120470027399044
18	16.746093825086756	25.673110050110825	32.255460216678664	25.325335908123748
19	16.702326331027162	27.82422323787781	28.79413160708028	26.679318824014754
20	19.648130048602617	27.95279025167788	33.50860046044586	18.890479239273635
21	19.757918441640633	32.256199532456705	29.004984466975504	18.980897558927165
22	16.742692972507804	24.521847520556676	30.143456833569672	28.59200267336585
23	17.346344305272357	29.178723674813433	29.511711501239834	23.963220518674376
24	21.81203339932959	23.898900045985442	27.120838206656504	27.168228348028467
25	16.213047149124428	32.91145510652801	24.84293236295673	26.03256538139083
26	15.898690080304481	24.683388018057048	29.606417852405954	29.811504049232518
27	20.523036340327753	28.031157724149452	27.57381698385778	23.871988951665013
28	13.747724755693099	27.611448156959696	32.018953099285675	26.62187398806153
29	18.082111367571542	24.148123394760617	27.177395863676086	30.592369373991758
30	17.572353138617274	27.953233841144698	33.494479529085424	20.979933491152607
31	21.700248853690887	29.966760362619603	22.27351430797825	26.059476475711257
32	20.691748200875054	34.928456412159676	22.789113131578986	21.590682255386284
33	19.131496182912638	25.0241386601528	27.410132470601106	28.43423268633345
34	23.279797013459984	25.905033409680012	25.087941611797117	25.727227965062895
35	15.961901579326366	27.909096689196083	27.940074020295697	28.188927711181854
36	18.284609959174983	26.301898119328527	26.282601977521843	29.13088994397465
37	17.96182469048545	29.017257108890863	32.924245269488	20.096672931135693
38	16.52947430212287	32.342847341642255	25.946065435360882	25.18161292087399
39	23.791329600281532	26.655882513851083	27.377454713212018	22.17533317265537
40	20.61086705475816	23.852766741436135	33.20732928089711	22.32903692290859
41	21.25163203958001	25.801824927066498	23.9067367932326	29.039806240120893
42	18.08425538332784	25.79420997455275	30.429054518624106	25.692480123495308
43	21.56539765577753	22.202392130131408	26.461442464228206	29.77076774986286
44	20.05674987912187	22.132970378574036	30.053112445493944	27.75716729681015
45	19.19448588720111	22.105541763208986	27.162609548115412	31.53736280147449
46	26.395717291561006	24.6034679824516	26.613963309236567	22.386851416750826
47	15.474248892135305	22.488507336230466	38.096572384189884	23.940671387444347
48	19.714964194936872	23.545433172507508	28.47666941199259	28.262933220563035
49	19.538045929253396	18.99302233768692	36.863393666429594	24.605538066630096
50	21.468473357277308	18.50041623478303	31.676132373011423	28.354978034928237
51	18.803683566932474	18.698478931718274	27.436230317565702	35.06160718378355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1411.0
1	1484.0
2	1557.0
3	2248.0
4	2939.0
5	2189.0
6	1439.0
7	1612.5
8	1786.0
9	1876.0
10	1966.0
11	2034.0
12	2102.0
13	2252.0
14	2402.0
15	2477.5
16	2553.0
17	2753.5
18	2954.0
19	3539.5
20	4125.0
21	3806.0
22	3487.0
23	4022.0
24	4557.0
25	5605.5
26	7019.0
27	7384.0
28	8800.0
29	10216.0
30	12143.5
31	14071.0
32	16802.5
33	19534.0
34	21235.0
35	22936.0
36	25799.5
37	28663.0
38	32970.5
39	37278.0
40	46221.0
41	55164.0
42	69060.0
43	82956.0
44	168055.0
45	253154.0
46	226938.5
47	200723.0
48	189217.5
49	177712.0
50	140995.0
51	104278.0
52	87529.5
53	70781.0
54	62399.0
55	54017.0
56	48052.0
57	42087.0
58	37165.5
59	32244.0
60	31414.5
61	30585.0
62	27272.0
63	23959.0
64	20684.0
65	17409.0
66	14004.5
67	10600.0
68	8917.5
69	7235.0
70	5422.0
71	3609.0
72	3218.5
73	2828.0
74	2197.0
75	1270.0
76	974.0
77	654.0
78	334.0
79	257.5
80	181.0
81	150.0
82	119.0
83	80.0
84	41.0
85	33.0
86	25.0
87	13.5
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1352602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.945609871496227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.17507215835298	11.968902707157786
2	12.555937052027263	4.7575976991754585
3	6.308052467490217	3.585297032939963
4	3.834227142101964	2.9056708637186293
5	2.5225874269775277	2.389597862912886
6	1.8228662205303021	2.0721187357257547
7	1.3603307611630724	1.8040607128033754
8	0.9939488460295335	1.506477365527953
9	0.7866149838495533	1.341261054277825
>10	4.813129682692972	17.70738786578296
>50	1.1675084007757635	16.313811388135736
>100	0.6337452394953959	17.081716601611276
>500	0.014170701007349304	1.8062983855526342
>1k	0.0074789810872121325	2.677268157439394
>5k	0.0027554140847623645	3.5623327394029243
>10k+	0.0015745223341499224	8.52020082783547
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	36035	2.664124406144601	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	31453	2.325369916649539	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	30948	2.288034469858835	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	15813	1.1690800398047616	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	8385	0.6199162798812954	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT	8255	0.6103051747668568	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT	8120	0.6003244117634012	No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	6737	0.49807703966133426	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC	5870	0.4339783617058085	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA	5397	0.39900872540481236	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	5004	0.3699536153280862	No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	4095	0.3027498111048187	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	3644	0.2694066695154968	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	3027	0.22379088601081473	No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2852	0.21085285989522418	TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1996	0.14756742929553557	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATG	1754	0.12967598746711895	No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1674	0.12376146124284895	No Hit
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1638	0.12109992444192749	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1635	0.12087812970851737	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTAATGAC	1577	0.11659009819592163	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC	1467	0.10845762463755045	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT	1466	0.10838369305974707	No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	1465	0.1083097614819437	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTAT	1445	0.1068311299258762	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTAATGACTCGT	1422	0.10513070363639858	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4786315560674906E-4	0.0	0.0	0.5263189023822233	0.0
2	1.4786315560674906E-4	0.0	0.0	2.954675506911863	0.0
3	1.4786315560674906E-4	0.0	0.0	3.9306462654942105	0.0
4	1.4786315560674906E-4	0.0	0.0	5.447426515708242	0.0
5	1.4786315560674906E-4	0.0	0.0	10.767542854439073	0.0
6	2.217947334101236E-4	0.0	0.0	12.314265393663472	0.0
7	2.217947334101236E-4	0.0	0.0	14.452662350048277	0.0
8	2.217947334101236E-4	0.0	0.0	16.99147273181616	0.0
9	2.217947334101236E-4	0.0	0.0	17.78268847746787	0.0
10	2.957263112134981E-4	0.0	0.0	22.014236264621818	0.0
11	2.957263112134981E-4	0.0	0.0	24.766117453619025	0.0
12	2.957263112134981E-4	0.0	0.0	29.942658668255703	0.0
13	2.957263112134981E-4	0.0	0.0	30.88661705364919	0.0
14	3.6965788901687267E-4	0.0	0.0	31.3293932731136	0.0
15	3.6965788901687267E-4	0.0	0.0	32.37996099369955	0.0
16	3.6965788901687267E-4	0.0	0.0	33.67871702097143	0.0
17	3.6965788901687267E-4	0.0	0.0	35.13413406160866	0.0
18	3.6965788901687267E-4	0.0	0.0	36.625260054324926	0.0
19	3.6965788901687267E-4	0.0	0.0	38.3708585378404	0.0
20	3.6965788901687267E-4	0.0	0.0	39.38179893272374	0.0
21	3.6965788901687267E-4	0.0	0.0	40.40471624321123	0.0
22	3.6965788901687267E-4	0.0	0.0	41.56130184636722	0.0
23	3.6965788901687267E-4	0.0	0.0	42.52174697361086	0.0
24	3.6965788901687267E-4	0.0	0.0	43.41136564931887	0.0
25	3.6965788901687267E-4	0.0	0.0	44.11024085429417	0.0
26	3.6965788901687267E-4	0.0	0.0	44.761060533697275	0.0
27	3.6965788901687267E-4	0.0	0.0	45.41735114985783	0.0
28	3.6965788901687267E-4	0.0	0.0	46.00680761968413	0.0
29	3.6965788901687267E-4	0.0	0.0	46.62997688898878	0.0
30	3.6965788901687267E-4	0.0	0.0	47.35849865666323	0.0
31	3.6965788901687267E-4	0.0	0.0	48.035416183030925	0.0
32	3.6965788901687267E-4	0.0	0.0	48.64350341046369	0.0
33	3.6965788901687267E-4	0.0	0.0	49.22741501195473	0.0
34	3.6965788901687267E-4	0.0	0.0	49.79705781892974	0.0
35	3.6965788901687267E-4	0.0	0.0	50.41800914090028	0.0
36	3.6965788901687267E-4	0.0	0.0	50.99770664245654	0.0
37	3.6965788901687267E-4	0.0	0.0	51.56882808098761	0.0
38	3.6965788901687267E-4	0.0	0.0	52.15177857196721	0.0
39	3.6965788901687267E-4	0.0	0.0	52.72267821576487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	40	6.8157533E-9	45.000004	25
CTTAACG	20	7.033729E-4	45.000004	1
AACGTAA	25	3.8911723E-5	45.000004	10
CGAACTT	20	7.033729E-4	45.000004	45
ACGTTCA	40	6.8157533E-9	45.000004	26
GACGTAA	20	7.033729E-4	45.000004	1
CGGCACG	25	3.8911723E-5	45.000004	2
CCGGATT	20	7.033729E-4	45.000004	44
ATAGCGC	25	3.8911723E-5	45.000004	12
ACATTCG	25	3.8911723E-5	45.000004	14
GCCGGAT	20	7.033729E-4	45.000004	45
TTCCGTA	20	7.033729E-4	45.000004	38
CGCGATA	20	7.033729E-4	45.000004	11
ATATGCG	20	7.033729E-4	45.000004	2
TGCGGTA	20	7.033729E-4	45.000004	18
TTATCCG	20	7.033729E-4	45.000004	1
CCGTCCG	20	7.033729E-4	45.000004	23
TCGGCCC	25	3.8911723E-5	45.000004	14
TCGGATC	25	3.8911723E-5	45.000004	29
CTAACGG	40	6.8157533E-9	45.000004	2
>>END_MODULE