SRR2934989.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934989.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955855
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	13342	1.3958184034189285	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	11670	1.220896474883743	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	8038	0.8409225248599421	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	5398	0.5647300061201751	No Hit
ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	3950	0.4132425943265453	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2409	0.2520256733500374	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2269	0.23737910038656493	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2024	0.21174759770048804	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1930	0.20191347013929936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1706	0.1784789533977434	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1680	0.17575887556166991	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	1645	0.1720972323208018	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1623	0.1697956279979704	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1596	0.16697093178358643	No Hit
TGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1468	0.15357977935984013	No Hit
AAAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1461	0.15284745071166653	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1368	0.14311794152878837	No Hit
GACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	1255	0.13129606477969985	No Hit
GGCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	1220	0.12763442153883173	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC	1187	0.12418201505458465	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	1125	0.11769567559933253	No Hit
GGACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1119	0.11706796532946943	No Hit
GAAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1075	0.11246475668380664	No Hit
GAAGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1049	0.10974467884773317	No Hit
GTACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1038	0.10859387668631748	No Hit
GAGCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1019	0.10660612749841764	No Hit
GTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1017	0.1063968907417966	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	964	0.10085211669133916	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	960	0.1004336431780971	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCA	20	7.0326886E-4	45.000004	36
GTTGAGT	20	7.0326886E-4	45.000004	12
AACGTTA	20	7.0326886E-4	45.000004	30
CGGCGTG	20	7.0326886E-4	45.000004	20
TACCCGG	20	7.0326886E-4	45.000004	44
CGTATAA	20	7.0326886E-4	45.000004	11
GATCGGT	40	6.8121153E-9	45.000004	18
ATCTACG	20	7.0326886E-4	45.000004	20
ATCTACC	20	7.0326886E-4	45.000004	35
GTCGACC	20	7.0326886E-4	45.000004	17
TTTACGA	20	7.0326886E-4	45.000004	12
ACGCATG	40	6.8121153E-9	45.000004	1
TCCGATA	20	7.0326886E-4	45.000004	32
GAGTCGA	20	7.0326886E-4	45.000004	15
CATGAGG	20	7.0326886E-4	45.000004	2
CGCATCG	20	7.0326886E-4	45.000004	21
CGGCACG	20	7.0326886E-4	45.000004	43
CCGTGTT	20	7.0326886E-4	45.000004	32
CACGCAT	20	7.0326886E-4	45.000004	12
CGACCGA	35	1.211647E-7	45.000004	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content