##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934989.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955855
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9716452809265	33.0	31.0	34.0	30.0	34.0
2	32.13510521993398	33.0	31.0	34.0	30.0	34.0
3	32.171069879845795	34.0	31.0	34.0	30.0	34.0
4	35.765730157816826	37.0	35.0	37.0	35.0	37.0
5	35.673196248384954	37.0	35.0	37.0	33.0	37.0
6	35.59918397664918	37.0	35.0	37.0	33.0	37.0
7	35.93288312557867	37.0	35.0	37.0	35.0	37.0
8	35.66795068289647	37.0	35.0	37.0	35.0	37.0
9	37.34569573837036	39.0	37.0	39.0	34.0	39.0
10	37.22486988089198	39.0	37.0	39.0	34.0	39.0
11	37.158191357475765	39.0	37.0	39.0	34.0	39.0
12	37.07376432617918	39.0	37.0	39.0	34.0	39.0
13	36.907639757076126	39.0	37.0	39.0	33.0	39.0
14	37.90984720485848	40.0	37.0	41.0	33.0	41.0
15	37.988971130558504	40.0	37.0	41.0	33.0	41.0
16	38.0743637894869	40.0	37.0	41.0	34.0	41.0
17	38.091367414513705	40.0	37.0	41.0	34.0	41.0
18	38.095062535635634	40.0	37.0	41.0	34.0	41.0
19	38.08392381689691	40.0	37.0	41.0	34.0	41.0
20	38.057436535876256	40.0	36.0	41.0	34.0	41.0
21	37.9537618153381	40.0	36.0	41.0	34.0	41.0
22	37.83321947366494	39.0	36.0	41.0	33.0	41.0
23	37.60866867882681	39.0	36.0	41.0	33.0	41.0
24	37.36277887336468	39.0	35.0	41.0	32.0	41.0
25	37.323848282427775	39.0	35.0	41.0	32.0	41.0
26	37.57685422998258	39.0	36.0	41.0	33.0	41.0
27	37.636698034743766	39.0	36.0	41.0	33.0	41.0
28	37.63127880274728	39.0	36.0	41.0	33.0	41.0
29	37.662405908846004	39.0	36.0	41.0	33.0	41.0
30	37.60952341097761	39.0	36.0	41.0	33.0	41.0
31	37.479756866888806	39.0	36.0	41.0	33.0	41.0
32	37.34732987743957	39.0	35.0	41.0	33.0	41.0
33	37.27216784972616	39.0	35.0	41.0	33.0	41.0
34	37.193481228847475	39.0	35.0	41.0	33.0	41.0
35	37.12101417055934	39.0	35.0	41.0	32.0	41.0
36	37.038782032839705	39.0	35.0	41.0	32.0	41.0
37	36.93349514309179	39.0	35.0	41.0	32.0	41.0
38	36.77669939478268	39.0	35.0	41.0	31.0	41.0
39	36.741930522934965	39.0	35.0	41.0	31.0	41.0
40	36.74756108405563	39.0	35.0	41.0	31.0	41.0
41	36.695502979008324	39.0	35.0	41.0	31.0	41.0
42	36.67501870053513	39.0	35.0	41.0	31.0	41.0
43	36.557751960286865	39.0	35.0	40.0	31.0	41.0
44	36.394505442771134	39.0	35.0	40.0	30.0	41.0
45	36.45273289358742	39.0	35.0	40.0	31.0	41.0
46	36.340036930287546	39.0	35.0	40.0	30.0	41.0
47	36.27566733448065	39.0	35.0	40.0	30.0	41.0
48	36.23258967102751	39.0	35.0	40.0	30.0	41.0
49	36.18626046837648	39.0	35.0	40.0	30.0	41.0
50	36.09580846467299	38.0	35.0	40.0	30.0	41.0
51	35.02043406165161	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	10.0
11	14.0
12	19.0
13	12.0
14	18.0
15	39.0
16	62.0
17	139.0
18	268.0
19	495.0
20	840.0
21	1395.0
22	2141.0
23	3194.0
24	4686.0
25	5622.0
26	6383.0
27	7321.0
28	9004.0
29	11039.0
30	13698.0
31	17849.0
32	24245.0
33	33886.0
34	60560.0
35	84320.0
36	76277.0
37	118652.0
38	220467.0
39	253074.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34298612237212	21.5168618671242	25.26690763766471	11.873244372838977
2	28.989857248222794	27.03276124516794	24.387590167964806	19.58979133864446
3	29.335620988539056	24.827510448760535	27.879228544078337	17.95764001862207
4	28.273325975174057	26.307023554827875	25.129334470186375	20.290315999811686
5	23.58066861605578	28.32751829513891	26.719115346993007	21.372697741812306
6	24.13200746975221	31.622264883272045	26.31162676347354	17.934100883502204
7	71.83976649177961	7.081094935947398	13.081272787190526	7.997865785082466
8	69.05566220818011	5.595827819072976	13.452981885327794	11.895528087419118
9	62.48405877460493	8.397612608606954	17.815777497633007	11.302551119155101
10	35.34312212626392	25.412327183516325	23.716567889481144	15.527982800738604
11	24.874693337378577	24.609381129983106	27.76833306306919	22.747592469569128
12	23.900696235307656	20.086310162106177	34.53933912570421	21.473654476881954
13	21.31965622400887	26.395530702878574	33.9831878266055	18.301625246507054
14	15.869352569165827	30.765335746530592	30.189725429066122	23.175586255237455
15	13.546719952294021	27.494546767030563	38.502911006376486	20.455822274298928
16	15.259008950102265	27.87399762516281	32.36871701251759	24.498276412217336
17	14.753074472592601	27.324855757410905	28.022555722363744	29.899514047632746
18	15.077600682111827	26.769122931825436	34.67220446615857	23.48107191990417
19	17.185137913177208	27.22306207531477	30.21127681499809	25.38052319650993
20	19.52294019490404	28.514471337179803	30.227911137149466	21.734677330766697
21	18.499667836648864	29.75231598934985	31.0011455712425	20.746870602758786
22	15.743182804923341	27.698447986357767	27.585878611295644	28.97249059742325
23	15.355257858147942	28.63363167007548	28.987555643899967	27.023554827876612
24	20.7469752211371	24.191221471875966	29.193653849171685	25.868149457815253
25	15.92302179723912	28.923110722860685	26.067656705253412	29.08621077464678
26	15.454540699164623	28.06942475584686	26.893618802014952	29.582415742973566
27	18.729828268932003	27.38773140277553	26.38098874829341	27.50145157999906
28	13.934017188799555	25.429484597559256	31.361346647765615	29.27515156587558
29	15.388840357585618	25.139063979369258	28.134811242290937	31.33728442075419
30	18.09887482934127	24.93987058706603	28.045153292078815	28.91610129151388
31	17.391445355205548	27.324437283897662	25.5811812461095	29.70293611478729
32	20.56786855746949	28.185132682258292	23.401143478874932	27.84585528139728
33	18.067803170983048	23.238357282223767	30.780086937872376	27.91375260892081
34	16.184149269502175	21.457752483378755	31.80974101720449	30.54835722991458
35	18.460122089647488	22.073431639736153	26.811074901527952	32.65537136908841
36	20.03818570808334	19.603810201338067	32.7666853236108	27.59131876696779
37	17.925836031615674	23.05454279153219	33.46124673721433	25.55837443963781
38	18.152230202279636	27.544972825376234	29.959251141648053	24.34354583069608
39	19.669824398052004	22.58407394426979	33.90503789800754	23.84106375967066
40	22.461775060024795	19.358689340956523	31.319394678063095	26.860140920955583
41	20.002929314592695	20.891871675097164	26.783874123167216	32.32132488714293
42	21.787091138300266	18.26689194490796	31.57058340438665	28.375433512405124
43	22.136830376992325	19.612493526737843	32.2534275596194	25.997248536650435
44	18.20317935251686	20.948051744249913	31.604270522202636	29.2444983810306
45	19.792541755810245	19.377102175539175	28.712304690564995	32.11805137808559
46	22.842795193831698	19.625570824026656	31.472451365531384	26.05918261661026
47	17.035010540301617	19.28231792478985	36.36691757641064	27.31575395849789
48	18.252977700592663	17.778533354954465	33.70689068948742	30.26159825496545
49	20.481453776985003	16.105999340904216	38.7477180116231	24.66482887048768
50	20.02050520214886	16.283013637005613	34.53410820678869	29.162372954056835
51	18.15913501524813	15.79538737570029	29.228387150770775	36.8170904582808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1995.0
1	1986.0
2	1977.0
3	2176.5
4	2376.0
5	1944.0
6	1512.0
7	1683.0
8	1854.0
9	2113.0
10	2372.0
11	2570.5
12	2769.0
13	2828.0
14	2887.0
15	2883.0
16	2879.0
17	2911.0
18	2943.0
19	3290.5
20	3638.0
21	3372.5
22	3107.0
23	3322.0
24	3537.0
25	3617.5
26	4109.5
27	4521.0
28	5641.0
29	6761.0
30	8197.5
31	9634.0
32	10147.0
33	10660.0
34	11389.0
35	12118.0
36	14083.0
37	16048.0
38	19469.0
39	22890.0
40	26896.5
41	30903.0
42	37665.5
43	44428.0
44	59986.0
45	75544.0
46	96693.5
47	117843.0
48	141136.0
49	164429.0
50	160854.0
51	157279.0
52	121394.5
53	85510.0
54	68260.5
55	51011.0
56	40906.5
57	30802.0
58	26946.5
59	23091.0
60	19410.0
61	15729.0
62	14282.0
63	12835.0
64	10859.0
65	8883.0
66	7763.0
67	6643.0
68	5563.5
69	4484.0
70	3797.0
71	3110.0
72	2217.0
73	1324.0
74	1074.5
75	669.0
76	513.0
77	400.5
78	288.0
79	183.5
80	79.0
81	84.0
82	89.0
83	54.0
84	19.0
85	16.0
86	13.0
87	6.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	955855.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.82547315057427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.399441121486	13.837227999584067
2	12.11188958459268	5.286954418624953
3	6.1851389014510065	4.049807490785741
4	3.8088662632155934	3.325212334477603
5	2.551874971846363	2.7847939340827637
6	1.8482530952659215	2.4203398983715294
7	1.4059319236775552	2.1479590616231796
8	1.0363274479541984	1.8094669512422008
9	0.8333671637712392	1.636976939171347
>10	5.334704761267273	22.73549946691878
>50	1.0353681030537083	16.163625115265866
>100	0.4217808463846668	13.880054105642753
>500	0.014011047588952276	2.0471577178982634
>1k	0.011112210156755254	3.8206791795924477
>5k	9.662791440656741E-4	1.4167925774019299
>10k+	9.662791440656741E-4	2.6374528093165432
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	13342	1.3958184034189285	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	11670	1.220896474883743	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	8038	0.8409225248599421	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	5398	0.5647300061201751	No Hit
ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	3950	0.4132425943265453	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2409	0.2520256733500374	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2269	0.23737910038656493	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2024	0.21174759770048804	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1930	0.20191347013929936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1706	0.1784789533977434	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1680	0.17575887556166991	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	1645	0.1720972323208018	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1623	0.1697956279979704	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1596	0.16697093178358643	No Hit
TGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1468	0.15357977935984013	No Hit
AAAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1461	0.15284745071166653	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1368	0.14311794152878837	No Hit
GACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	1255	0.13129606477969985	No Hit
GGCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	1220	0.12763442153883173	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC	1187	0.12418201505458465	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	1125	0.11769567559933253	No Hit
GGACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1119	0.11706796532946943	No Hit
GAAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1075	0.11246475668380664	No Hit
GAAGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1049	0.10974467884773317	No Hit
GTACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1038	0.10859387668631748	No Hit
GAGCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	1019	0.10660612749841764	No Hit
GTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1017	0.1063968907417966	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	964	0.10085211669133916	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	960	0.1004336431780971	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0461837831051781E-4	0.0	0.0	0.8862222826683963	0.0
2	1.0461837831051781E-4	0.0	0.0	4.678847733181288	0.0
3	1.0461837831051781E-4	0.0	0.0	6.431100951504151	0.0
4	1.0461837831051781E-4	0.0	0.0	8.355137547012884	0.0
5	1.0461837831051781E-4	0.0	0.0	13.11223982717044	0.0
6	1.0461837831051781E-4	0.0	0.0	15.435395535933797	0.0
7	1.0461837831051781E-4	0.0	0.0	17.960150859701525	0.0
8	1.0461837831051781E-4	0.0	0.0	21.56383551898562	0.0
9	1.0461837831051781E-4	0.0	0.0	22.851478519231474	0.0
10	1.0461837831051781E-4	0.0	0.0	25.512656208316116	0.0
11	1.0461837831051781E-4	0.0	0.0	29.599573157016493	0.0
12	1.0461837831051781E-4	0.0	0.0	32.8383489127535	0.0
13	1.0461837831051781E-4	0.0	0.0	34.05390985034341	0.0
14	1.0461837831051781E-4	0.0	0.0	34.521867856526356	0.0
15	1.0461837831051781E-4	0.0	0.0	35.364150420304334	0.0
16	2.0923675662103561E-4	0.0	0.0	37.10510485376966	0.0
17	2.0923675662103561E-4	0.0	0.0	39.176862599452846	0.0
18	2.0923675662103561E-4	0.0	0.0	41.473654476881954	0.0
19	2.0923675662103561E-4	0.0	0.0	42.7443492998415	0.0
20	2.0923675662103561E-4	0.0	0.0	43.846085441829565	0.0
21	2.0923675662103561E-4	0.0	0.0	45.13707623018136	0.0
22	2.0923675662103561E-4	0.0	0.0	46.4296362942078	0.0
23	2.0923675662103561E-4	0.0	0.0	47.64676650747237	0.0
24	2.0923675662103561E-4	0.0	0.0	48.55192471661497	0.0
25	2.0923675662103561E-4	0.0	0.0	49.388034796072624	0.0
26	2.0923675662103561E-4	0.0	0.0	50.11398172316931	0.0
27	3.1385513493155343E-4	0.0	0.0	50.781237740033795	0.0
28	3.1385513493155343E-4	0.0	0.0	51.451841545004214	0.0
29	3.1385513493155343E-4	0.0	0.0	52.206349289379666	0.0
30	3.1385513493155343E-4	0.0	0.0	53.01954794398732	0.0
31	3.1385513493155343E-4	0.0	0.0	53.76694163863766	0.0
32	4.1847351324207123E-4	0.0	0.0	54.41745871497246	0.0
33	4.1847351324207123E-4	0.0	0.0	55.05217841618237	0.0
34	4.1847351324207123E-4	0.0	0.0	55.65875577362675	0.0
35	4.1847351324207123E-4	0.0	0.0	56.364406735331194	0.0
36	5.230918915525891E-4	0.0	0.0	56.96732244953471	0.0
37	5.230918915525891E-4	0.0	0.0	57.52650768160443	0.0
38	5.230918915525891E-4	0.0	0.0	58.09270234502095	0.0
39	6.277102698631069E-4	0.0	0.0	58.67835602680323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCA	20	7.0326886E-4	45.000004	36
GTTGAGT	20	7.0326886E-4	45.000004	12
AACGTTA	20	7.0326886E-4	45.000004	30
CGGCGTG	20	7.0326886E-4	45.000004	20
TACCCGG	20	7.0326886E-4	45.000004	44
CGTATAA	20	7.0326886E-4	45.000004	11
GATCGGT	40	6.8121153E-9	45.000004	18
ATCTACG	20	7.0326886E-4	45.000004	20
ATCTACC	20	7.0326886E-4	45.000004	35
GTCGACC	20	7.0326886E-4	45.000004	17
TTTACGA	20	7.0326886E-4	45.000004	12
ACGCATG	40	6.8121153E-9	45.000004	1
TCCGATA	20	7.0326886E-4	45.000004	32
GAGTCGA	20	7.0326886E-4	45.000004	15
CATGAGG	20	7.0326886E-4	45.000004	2
CGCATCG	20	7.0326886E-4	45.000004	21
CGGCACG	20	7.0326886E-4	45.000004	43
CCGTGTT	20	7.0326886E-4	45.000004	32
CACGCAT	20	7.0326886E-4	45.000004	12
CGACCGA	35	1.211647E-7	45.000004	28
>>END_MODULE