FastQCFastQC Report
Sat 14 Jan 2017
SRR2934987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329980
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC173950.7465729319564975TruSeq Adapter, Index 16 (100% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC132740.5697044609824977TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT92680.3977716546923149TruSeq Adapter, Index 16 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC70250.30150473394621413TruSeq Adapter, Index 16 (100% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC50160.21528081786109754TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG37740.1619756392758736TruSeq Adapter, Index 16 (100% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG35240.1512459334414888No Hit
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG31770.1363531017433626TruSeq Adapter, Index 16 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG30620.1314174370595456TruSeq Adapter, Index 16 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG25770.11060180774083898TruSeq Adapter, Index 16 (100% over 22bp)
AGCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT23360.100258371316492TruSeq Adapter, Index 16 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA302.1664655E-645.00000418
GACGTTA302.1664655E-645.0000049
CCTTACG207.03478E-445.016
CGAACGC253.892044E-545.032
CTCCGTA253.892044E-545.040
AGGCGTA207.03478E-445.013
ATCGTAC253.892044E-545.042
CTACGTT351.2126475E-745.045
CGTACTA207.03478E-445.043
TCCGTAC253.892044E-545.022
TACTACG351.2126475E-745.01
ACCGTAC351.2126475E-745.031
ATCGGTC207.03478E-445.026
TGTCGAC207.03478E-445.044
TACGTAA207.03478E-445.033
CCATACG2850.042.631582
ACGTTAG1100.040.909091
GAGTACG556.184564E-1140.909091
ATACGGA2900.040.344834
GCTAACG850.039.7058831
Produced by FastQC (version 0.11.5)