##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934985.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1221913
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27093500110073	33.0	31.0	34.0	30.0	34.0
2	32.44692461738274	34.0	31.0	34.0	31.0	34.0
3	32.44559555385695	34.0	31.0	34.0	30.0	34.0
4	35.984536542290655	37.0	35.0	37.0	35.0	37.0
5	35.89950593863884	37.0	35.0	37.0	35.0	37.0
6	35.8426917464664	37.0	35.0	37.0	35.0	37.0
7	36.13057476268769	37.0	35.0	37.0	35.0	37.0
8	35.90273611951096	37.0	35.0	37.0	35.0	37.0
9	37.63546831893924	39.0	38.0	39.0	35.0	39.0
10	37.41029598670282	39.0	37.0	39.0	35.0	39.0
11	37.34773097593691	39.0	37.0	39.0	34.0	39.0
12	37.27625371037054	39.0	37.0	39.0	34.0	39.0
13	37.21579932450182	39.0	37.0	39.0	34.0	39.0
14	38.35106263702899	40.0	38.0	41.0	34.0	41.0
15	38.40756665981948	40.0	38.0	41.0	34.0	41.0
16	38.47617874595	40.0	38.0	41.0	34.0	41.0
17	38.4854265401874	40.0	38.0	41.0	34.0	41.0
18	38.49596084172932	40.0	38.0	41.0	34.0	41.0
19	38.497451127862625	40.0	38.0	41.0	34.0	41.0
20	38.468787057671044	40.0	38.0	41.0	34.0	41.0
21	38.3998893538247	40.0	37.0	41.0	34.0	41.0
22	38.34857882680682	40.0	37.0	41.0	34.0	41.0
23	38.1457583314033	40.0	37.0	41.0	34.0	41.0
24	37.904436731584	40.0	36.0	41.0	33.0	41.0
25	37.880325358679386	40.0	36.0	41.0	33.0	41.0
26	38.06232685960457	40.0	37.0	41.0	34.0	41.0
27	38.11110119951257	40.0	37.0	41.0	34.0	41.0
28	38.087994808141005	40.0	37.0	41.0	34.0	41.0
29	38.12852469856692	40.0	37.0	41.0	34.0	41.0
30	38.04257586260233	40.0	37.0	41.0	34.0	41.0
31	37.93603881782091	40.0	37.0	41.0	34.0	41.0
32	37.87837104605647	40.0	37.0	41.0	34.0	41.0
33	37.82594914695236	40.0	37.0	41.0	33.0	41.0
34	37.715474014925775	40.0	36.0	41.0	33.0	41.0
35	37.66903863040986	40.0	36.0	41.0	33.0	41.0
36	37.646752264686604	40.0	36.0	41.0	33.0	41.0
37	37.5839924773695	40.0	36.0	41.0	33.0	41.0
38	37.505433692906124	40.0	36.0	41.0	33.0	41.0
39	37.48486839897767	40.0	36.0	41.0	33.0	41.0
40	37.43415693261304	40.0	36.0	41.0	33.0	41.0
41	37.37334654758563	40.0	36.0	41.0	33.0	41.0
42	37.38541369148213	40.0	36.0	41.0	33.0	41.0
43	37.30757590761372	39.0	35.0	41.0	33.0	41.0
44	37.193429483113775	39.0	35.0	41.0	33.0	41.0
45	37.202007016866176	39.0	35.0	41.0	33.0	41.0
46	37.085361232755524	39.0	35.0	41.0	32.0	41.0
47	37.032464668106485	39.0	35.0	41.0	32.0	41.0
48	36.99632297880455	39.0	35.0	41.0	32.0	41.0
49	36.9606346769369	39.0	35.0	41.0	32.0	41.0
50	36.89622419926787	39.0	35.0	41.0	32.0	41.0
51	35.90202575797131	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	9.0
10	11.0
11	10.0
12	15.0
13	24.0
14	22.0
15	35.0
16	63.0
17	110.0
18	231.0
19	416.0
20	717.0
21	1213.0
22	1893.0
23	2669.0
24	3715.0
25	4626.0
26	5927.0
27	7174.0
28	8401.0
29	10732.0
30	13878.0
31	18105.0
32	24796.0
33	35465.0
34	67402.0
35	93910.0
36	87369.0
37	138389.0
38	266062.0
39	428215.0
40	301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.628669962591445	22.876587776707506	25.44968422465429	11.045058036046756
2	31.013828316745958	24.390770865028852	26.598374843380828	17.997025974844362
3	30.634095880803297	23.69906859162641	27.97449572923768	17.692339798332615
4	29.402093275053133	25.164803058810243	26.14686970348953	19.286233962647096
5	24.87779408190272	29.266649916974448	25.495022968083653	20.36053303303918
6	26.101121765624885	32.497239983534016	24.99523288482895	16.406405366012148
7	80.02222744172457	5.559724792190606	8.562393558297522	5.855654207787298
8	79.80363577439637	4.295968698262478	8.773865242451794	7.12653028488935
9	74.18195894470392	6.797619797808846	11.484205503992511	7.536215753494726
10	38.335626186152375	28.45685412954932	18.828018034017152	14.379501650281156
11	26.739383245779365	26.137458231478018	26.22011550740519	20.903043015337424
12	25.370136826435267	22.494236496379038	31.237084800636378	20.898541876549313
13	23.12660557666544	24.6539647258029	32.344283103625216	19.87514659390644
14	18.292709873779884	29.34914351512751	30.325399598825776	22.03274701226683
15	17.14246431619927	27.537803427903622	35.034982032272346	20.28475022362476
16	19.544026456875407	27.704591079724988	30.526968777646204	22.224413685753404
17	19.422577548483403	27.109867887484622	29.027189333446817	24.440365230585158
18	20.207248797582153	27.083515765852397	30.8019474381564	21.907287998409053
19	20.833970994661648	27.00724192311564	28.804587560652845	23.354199521569864
20	22.510031401580964	28.60760135950759	28.252911623004255	20.62945561590719
21	21.634027954527042	27.5957453599397	30.214344229089964	20.555882456443296
22	19.858124105398666	25.989739040340844	28.363885153853012	25.78825170040748
23	19.90632720987501	25.59134733814928	29.516422200271215	24.9859032517045
24	21.33130591130465	24.892688759347024	29.66610552469775	24.109899804650574
25	19.243104869168263	28.103064620803607	27.33165127140803	25.322179238620095
26	18.56736117874186	28.014351267234243	29.299958344006487	24.118329210017407
27	19.670385698490808	27.569638754968643	28.521425011436985	24.238550535103563
28	17.002683497106585	28.04004867777002	29.887479714185872	25.069788110937523
29	18.663194515485145	24.714607341111847	31.70111129024734	24.921086853155668
30	20.93021352584022	25.683825280523244	28.20405380743146	25.181907386205072
31	19.51186377426216	29.28637308875509	26.33395339930093	24.867809737681814
32	20.624217927135565	28.360775276144867	27.424129213781995	23.590877582937576
33	22.50798542940455	26.09522936575681	25.499033073549427	25.897752131289213
34	19.348677033471287	25.88097515944261	28.348090248651094	26.422257558435014
35	18.29860227364796	25.95389360781005	28.269606755963807	27.477897362578187
36	20.489592957927446	26.88612037027186	26.524801683916937	26.099484987883752
37	19.67660545390711	27.134746909149833	28.911550986035834	24.277096650907225
38	19.29916450680204	28.33016753238569	27.65417832529812	24.71648963551415
39	22.43007480892666	23.96193509685223	28.868749248105225	24.739240846115884
40	22.206409130600953	23.803085817075356	29.466173123618454	24.524331928705234
41	18.577100006301595	26.797488855589556	26.050217977875672	28.575193160233177
42	20.02663037384822	24.86003504341144	28.296695427579543	26.8166391551608
43	21.92758404239909	23.781398512005357	28.646147475311253	25.6448699702843
44	20.33393539474578	24.321289649917794	28.39915771417441	26.94561724116201
45	20.818667122782063	23.180864758783972	27.859266576261977	28.14120154217199
46	22.56167173931368	24.27554171205315	28.139319247769684	25.023467300863484
47	17.948741031480964	24.975673390822422	31.03805262731471	26.0375329503819
48	18.62693988851907	23.106964243771856	30.544727816137485	27.721368051571595
49	20.903534048659765	21.298897712030236	32.07544235964426	25.722125879665736
50	20.033914034796258	21.443834381007488	30.914148552311005	27.60810303188525
51	18.895207760290624	21.45815618624239	28.177619846912176	31.469016206554805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	877.0
1	1086.5
2	1296.0
3	1846.5
4	2397.0
5	1836.5
6	1276.0
7	1357.5
8	1439.0
9	1584.5
10	1730.0
11	1794.5
12	1859.0
13	1883.0
14	1907.0
15	2003.5
16	2100.0
17	2367.0
18	2634.0
19	2670.5
20	2707.0
21	2941.0
22	3175.0
23	3550.5
24	3926.0
25	5187.5
26	7192.5
27	7936.0
28	11074.5
29	14213.0
30	17224.5
31	20236.0
32	22757.5
33	25279.0
34	28190.5
35	31102.0
36	32856.0
37	34610.0
38	39235.5
39	43861.0
40	50662.5
41	57464.0
42	65438.0
43	73412.0
44	82750.5
45	92089.0
46	105739.5
47	119390.0
48	136841.0
49	154292.0
50	152247.0
51	150202.0
52	123264.0
53	96326.0
54	81276.0
55	66226.0
56	56684.5
57	47143.0
58	42876.0
59	38609.0
60	35669.5
61	32730.0
62	29840.0
63	26950.0
64	22973.0
65	18996.0
66	16051.0
67	13106.0
68	10914.0
69	8722.0
70	7715.0
71	6708.0
72	5194.0
73	3680.0
74	3046.5
75	1698.0
76	983.0
77	828.0
78	673.0
79	513.0
80	353.0
81	311.5
82	270.0
83	189.0
84	108.0
85	70.5
86	33.0
87	20.5
88	8.0
89	7.5
90	7.0
91	5.0
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1221913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.92793081503154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.33422084411856	14.107163437087916
2	13.440589866723087	5.894486494214357
3	6.26585260464636	4.121915472356116
4	3.529787749384304	3.0960376624097967
5	2.2118513450251425	2.425066163342289
6	1.5160459409420262	1.9946250303231685
7	1.0579228489470565	1.6238641327546481
8	0.7925896033368759	1.3903879989347443
9	0.587001644535072	1.1584558304707318
>10	4.196471136258333	19.984077649103824
>50	1.4853102212976295	23.56247327639658
>100	0.5702866363444397	16.781870537575934
>500	0.006789126623146827	0.9218454036626978
>1k	0.004526084415431219	1.7897662421730471
>5k	7.543474025718698E-4	1.1479646691941725
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	7332	0.6000427198990436	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	6548	0.5358810324466635	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	4976	0.4072303019936771	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	3075	0.25165457769906696	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2328	0.19052092906778142	TruSeq Adapter, Index 16 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1498	0.1225946528107975	No Hit
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1395	0.11416524744396696	No Hit
GGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1382	0.11310134191223106	No Hit
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1335	0.10925491422057053	No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1302	0.1065542309477025	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	1292	0.10573584207713642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4238435960661684	0.0
2	0.0	0.0	0.0	2.081326575623633	0.0
3	8.183888705660714E-5	0.0	0.0	2.931632612141781	0.0
4	8.183888705660714E-5	0.0	0.0	3.9216376288655574	0.0
5	8.183888705660714E-5	0.0	0.0	6.4497226889312085	0.0
6	8.183888705660714E-5	0.0	0.0	7.733365632414092	0.0
7	8.183888705660714E-5	0.0	0.0	9.073886602401316	0.0
8	8.183888705660714E-5	0.0	0.0	10.993745053862263	0.0
9	8.183888705660714E-5	0.0	0.0	11.676854244123763	0.0
10	8.183888705660714E-5	0.0	0.0	13.24357789793545	0.0
11	8.183888705660714E-5	0.0	0.0	15.670264576937965	0.0
12	8.183888705660714E-5	0.0	0.0	17.538891885101478	0.0
13	8.183888705660714E-5	0.0	0.0	18.232721969567393	0.0
14	8.183888705660714E-5	0.0	0.0	18.51662106876676	0.0
15	8.183888705660714E-5	0.0	0.0	19.04677337911946	0.0
16	8.183888705660714E-5	0.0	0.0	20.169521070649058	0.0
17	8.183888705660714E-5	0.0	0.0	21.523136262565338	0.0
18	8.183888705660714E-5	0.0	0.0	22.998200362873625	0.0
19	8.183888705660714E-5	0.0	0.0	23.89441801503053	0.0
20	8.183888705660714E-5	0.0	0.0	24.75045277364264	0.0
21	8.183888705660714E-5	0.0	0.0	25.78792434485925	0.0
22	8.183888705660714E-5	0.0	0.0	26.881619231483747	0.0
23	8.183888705660714E-5	0.0	0.0	27.884636631249524	0.0
24	8.183888705660714E-5	0.0	0.0	28.704171246234388	0.0
25	8.183888705660714E-5	0.0	0.0	29.365183936990604	0.0
26	8.183888705660714E-5	0.0	0.0	29.963835395809685	0.0
27	8.183888705660714E-5	0.0	0.0	30.565678571223973	0.0
28	8.183888705660714E-5	0.0	0.0	31.15385465249981	0.0
29	1.6367777411321427E-4	0.0	0.0	31.787369477205004	0.0
30	2.455166611698214E-4	0.0	0.0	32.511725466543034	0.0
31	3.2735554822642854E-4	0.0	0.0	33.168073340737024	0.0
32	3.2735554822642854E-4	0.0	0.0	33.7722080049889	0.0
33	3.2735554822642854E-4	0.0	0.0	34.35236387533319	0.0
34	3.2735554822642854E-4	0.0	0.0	34.924008501423586	0.0
35	4.091944352830357E-4	0.0	0.0	35.5435288764421	0.0
36	4.091944352830357E-4	0.0	0.0	36.132032313266166	0.0
37	4.091944352830357E-4	0.0	0.0	36.71709851683385	0.0
38	4.091944352830357E-4	0.0	0.0	37.28833394848897	0.0
39	4.091944352830357E-4	0.0	0.0	37.85948754125703	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCCG	30	2.1656142E-6	45.000004	37
GTATGCG	30	2.1656142E-6	45.000004	1
TATTCGA	30	2.1656142E-6	45.000004	24
CGGGTCG	35	1.2120108E-7	45.0	6
GTCGACT	25	3.8909508E-5	45.0	30
TAGAACG	35	1.2120108E-7	45.0	14
GACCGAA	25	3.8909508E-5	45.0	9
CGCCTAG	20	7.033462E-4	45.0	37
TACGCTA	20	7.033462E-4	45.0	17
ACTACGC	20	7.033462E-4	45.0	15
ACGTCAA	35	1.2120108E-7	45.0	36
CGTAAGT	20	7.033462E-4	45.0	14
ATGCGAT	20	7.033462E-4	45.0	42
ATACGAC	20	7.033462E-4	45.0	28
ATCGCTA	20	7.033462E-4	45.0	12
TCGAATC	25	3.8909508E-5	45.0	11
ATCGCAA	20	7.033462E-4	45.0	15
ACGACTC	20	7.033462E-4	45.0	12
TGGTACG	75	0.0	45.0	1
CGTAAGG	90	0.0	44.999996	2
>>END_MODULE