FastQCFastQC Report
Sat 14 Jan 2017
SRR2934984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930497
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC110430.5720288609617109TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC94180.4878536459782118TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT66420.34405647872024664TruSeq Adapter, Index 15 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC63690.3299150426030188No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC59450.30795178650886273No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG51970.26920528755030443No Hit
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC37830.19595990048158585TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC24410.12644412293829TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT302.1662709E-645.00000442
CCGTACA302.1662709E-645.00000415
CGGAAAT253.8917948E-545.032
ACGTTCA207.03448E-445.015
CGGCATA253.8917948E-545.06
CACGATA253.8917948E-545.010
TACGACG551.8189894E-1245.01
GTCGTGC551.8189894E-1245.022
CGTGTCG207.03448E-445.02
TCGAGTA253.8917948E-545.021
ACCCGGT253.8917948E-545.029
CGGTCAA207.03448E-445.045
TACGTTG207.03448E-445.01
TAATCGC253.8917948E-545.030
CGTCATA700.041.78571338
TCGCGTC650.041.53846418
CGCGTCG603.6379788E-1241.25000419
CGTAAGG1800.041.252
TCGATAG1150.041.086961
CCGCTCG556.184564E-1140.90909219