##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934984.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1930497
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.339066571976026	33.0	31.0	34.0	30.0	34.0
2	32.510447827683755	34.0	31.0	34.0	31.0	34.0
3	32.51022664111884	34.0	31.0	34.0	31.0	34.0
4	36.0257586517876	37.0	35.0	37.0	35.0	37.0
5	35.95394346637161	37.0	35.0	37.0	35.0	37.0
6	35.893140989082085	37.0	35.0	37.0	35.0	37.0
7	36.18185109844771	37.0	35.0	37.0	35.0	37.0
8	35.95613875597838	37.0	36.0	37.0	35.0	37.0
9	37.729568603318214	39.0	38.0	39.0	35.0	39.0
10	37.47875857874941	39.0	37.0	39.0	35.0	39.0
11	37.40617882338071	39.0	37.0	39.0	35.0	39.0
12	37.37235333699042	39.0	37.0	39.0	35.0	39.0
13	37.334332557885354	39.0	37.0	39.0	34.0	39.0
14	38.53388013552986	40.0	38.0	41.0	34.0	41.0
15	38.55914202404873	40.0	38.0	41.0	34.0	41.0
16	38.61984504508425	40.0	38.0	41.0	35.0	41.0
17	38.63024702965092	40.0	38.0	41.0	35.0	41.0
18	38.63636514327658	40.0	38.0	41.0	35.0	41.0
19	38.616707511070985	40.0	38.0	41.0	35.0	41.0
20	38.5891275666318	40.0	38.0	41.0	35.0	41.0
21	38.54656236192027	40.0	38.0	41.0	34.0	41.0
22	38.49271405239169	40.0	38.0	41.0	34.0	41.0
23	38.292446970909566	40.0	37.0	41.0	34.0	41.0
24	38.09173440828968	40.0	37.0	41.0	34.0	41.0
25	38.06895581811316	40.0	37.0	41.0	34.0	41.0
26	38.24955438936191	40.0	37.0	41.0	34.0	41.0
27	38.26449561952181	40.0	37.0	41.0	34.0	41.0
28	38.2701226678933	40.0	37.0	41.0	34.0	41.0
29	38.33000206682528	40.0	37.0	41.0	34.0	41.0
30	38.22185219661051	40.0	37.0	41.0	34.0	41.0
31	38.1768637817101	40.0	37.0	41.0	34.0	41.0
32	38.07019746728433	40.0	37.0	41.0	34.0	41.0
33	37.98641282529836	40.0	37.0	41.0	34.0	41.0
34	37.94889709748319	40.0	37.0	41.0	34.0	41.0
35	37.851231056044114	40.0	37.0	41.0	34.0	41.0
36	37.7855971804152	40.0	37.0	41.0	33.0	41.0
37	37.76469375502785	40.0	37.0	41.0	33.0	41.0
38	37.68983064982748	40.0	37.0	41.0	33.0	41.0
39	37.67957474163389	40.0	36.0	41.0	33.0	41.0
40	37.64168087285295	40.0	36.0	41.0	33.0	41.0
41	37.57144507347072	40.0	36.0	41.0	33.0	41.0
42	37.56104516090934	40.0	36.0	41.0	33.0	41.0
43	37.515244519934505	40.0	36.0	41.0	33.0	41.0
44	37.45288493066811	40.0	36.0	41.0	33.0	41.0
45	37.44973289261781	40.0	36.0	41.0	33.0	41.0
46	37.34072832022013	39.0	36.0	41.0	33.0	41.0
47	37.26703952401895	39.0	35.0	41.0	33.0	41.0
48	37.222792369011714	39.0	35.0	41.0	33.0	41.0
49	37.19127198850866	39.0	35.0	41.0	33.0	41.0
50	37.13737602285836	39.0	35.0	41.0	33.0	41.0
51	36.10699265525924	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	17.0
10	23.0
11	21.0
12	18.0
13	26.0
14	36.0
15	40.0
16	74.0
17	153.0
18	282.0
19	517.0
20	947.0
21	1608.0
22	2672.0
23	3797.0
24	5166.0
25	6681.0
26	8194.0
27	10154.0
28	12340.0
29	15614.0
30	19935.0
31	26369.0
32	36298.0
33	52268.0
34	97463.0
35	140515.0
36	128263.0
37	211231.0
38	422105.0
39	727019.0
40	643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66849106732619	21.49772830519809	26.843916359362378	10.98986426811334
2	30.193053913059693	26.143992971758053	25.96911572512156	17.693837390060693
3	30.620819405572764	25.215320199927792	27.42252383712588	16.741336557373568
4	28.30115768115672	25.379267618649497	27.491625213610792	18.82794948658299
5	25.809312316983657	29.25619672032642	25.55150305853881	19.382987904151108
6	25.61024440856422	34.21688818993244	24.592475409182196	15.580391992321148
7	80.77655650332531	4.992341350439809	9.05072631555501	5.180375830679871
8	81.66472157169889	4.500343693877794	7.73137694593672	6.103557788486592
9	76.35743541689006	6.416223387034531	9.876627624906954	7.3497135711684605
10	37.4849585365841	30.17269646106676	17.781690414437318	14.560654587911817
11	25.13368319142687	26.29250395105509	27.924674319618216	20.649138537899823
12	23.556317362834545	23.216922896021078	31.595076293824853	21.631683447319524
13	22.244530812531696	24.73606537591097	33.13566402848593	19.883739783071405
14	19.109483205620105	28.85091248523049	29.80310251712383	22.236501792025575
15	16.683579409861814	27.881317608885176	34.80870470143181	20.626398279821206
16	19.742532622428318	27.749175471394157	30.299917585989515	22.208374320188014
17	20.069909458548757	26.90861472460201	28.13073524589782	24.89074057095142
18	19.83463325765334	26.539020780659072	31.673087293064945	21.953258668622638
19	19.82033642113922	28.156117310723612	29.515767183269386	22.50777908486778
20	21.71544426124464	28.526177455857223	29.79590229873447	19.962475984163664
21	21.26022469861388	28.306803895577147	30.439674342928274	19.9932970628807
22	20.148438459111826	25.53839762506753	29.312762464795334	25.000401451025304
23	18.23737617825876	27.61672253310935	29.81465394662618	24.33124734200571
24	20.26804496458684	25.844329206416795	30.104061285772527	23.783564543223843
25	18.554082187125907	29.023873126971967	28.376837674443422	24.045207011458707
26	18.717356204127743	28.486135953591223	29.060651220903218	23.73585662137781
27	20.31140167531988	29.002376072068486	28.59885304147067	22.087369211140963
28	16.36107178617734	27.33187360560519	30.539285997336435	25.76776861088103
29	17.770397985596457	25.28794398540894	31.077023170717176	25.86463485827743
30	19.767034085005054	29.183728335242172	28.319961129180726	22.729276450572055
31	20.605315625976107	28.44733765450037	25.70048023902653	25.246866480496994
32	19.682030067904794	28.574973180481507	27.052204691330783	24.69079206028292
33	20.172214719836397	30.393106023992782	25.707732257548187	23.726946998622633
34	20.354447585259134	25.505867141984677	29.122604179131073	25.017081093625116
35	17.48420225465256	27.112707245854306	30.033820306377063	25.369270193116073
36	19.146727500742035	30.58958392579735	28.56647795878471	21.69721061467591
37	19.447634469258436	27.469610157384345	29.822682967132298	23.260072406224925
38	17.81510149976923	28.83112483469283	27.248837993532238	26.10493567200571
39	21.075453626708562	27.474168569026524	27.989269084593243	23.46110871967167
40	21.746731541152357	25.594341767948876	30.842834772600007	21.81609191829876
41	20.243077300819426	26.976213897250297	28.699189897731	24.08151890419928
42	19.184696997716134	26.133788345695436	31.134262316905957	23.547252339682476
43	20.266439160485618	26.382273580326725	29.05117179669277	24.300115462494894
44	19.4547310873832	25.21832460760105	27.744565259619673	27.58237904539608
45	20.14201524270693	24.187657375276935	28.196832214709477	27.473495167306655
46	22.63867801918366	25.683541595765234	28.404395344825712	23.273385040225392
47	18.038878071294594	25.95968810104341	32.32986117046543	23.671572657196567
48	18.290937515054413	24.83660943270049	30.616364594195172	26.25608845804992
49	19.82934964415899	23.500528620350096	32.92903330075105	23.74108843473986
50	20.09384111967022	23.069499719502286	30.823979524443708	26.01267963638379
51	18.854678354848517	22.732695259303693	28.42796440502109	29.9846619808267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	861.0
1	1220.0
2	1579.0
3	2698.5
4	3818.0
5	2793.5
6	1769.0
7	1899.5
8	2030.0
9	2187.5
10	2345.0
11	2479.0
12	2613.0
13	2758.5
14	2904.0
15	3122.5
16	3341.0
17	3437.0
18	3533.0
19	3680.5
20	3828.0
21	5152.5
22	6477.0
23	6600.0
24	6723.0
25	8459.5
26	13616.0
27	17036.0
28	21578.5
29	26121.0
30	29009.0
31	31897.0
32	35876.0
33	39855.0
34	46130.0
35	52405.0
36	59068.5
37	65732.0
38	71906.0
39	78080.0
40	89377.5
41	100675.0
42	118956.5
43	137238.0
44	175538.5
45	213839.0
46	234277.0
47	254715.0
48	247464.5
49	240214.0
50	204450.0
51	168686.0
52	140813.0
53	112940.0
54	99574.5
55	86209.0
56	78915.0
57	71621.0
58	62567.0
59	53513.0
60	47360.5
61	41208.0
62	35785.0
63	30362.0
64	26406.0
65	22450.0
66	18061.5
67	13673.0
68	10990.5
69	8308.0
70	6500.0
71	4692.0
72	3999.0
73	3306.0
74	2487.5
75	1425.0
76	1181.0
77	788.0
78	395.0
79	307.5
80	220.0
81	181.5
82	143.0
83	88.5
84	34.0
85	31.5
86	29.0
87	24.5
88	20.0
89	15.0
90	10.0
91	5.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1930497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.8264243036892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.23080320909234	12.338143091828483
2	13.499607625979706	5.352978974519843
3	6.66882393119664	3.9665679859950362
4	3.8456156320875916	3.049792289226741
5	2.455848046656295	2.434534269919701
6	1.6726225021933865	1.989727405703064
7	1.2291718674991623	1.7059058091037502
8	0.9152539781222244	1.4516970972712555
9	0.7141897112491945	1.2743845423700222
>10	4.535176450233322	18.47455872650246
>50	1.4096138954122133	20.59969800081422
>100	0.8060940968572523	21.970902833682644
>500	0.01083601831156523	1.5169240470937353
>1k	0.004757276331906686	1.5364168014382333
>5k	0.0013214656477518573	1.7591162798363948
>10k+	2.642931295503715E-4	0.5786518446943286
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	11043	0.5720288609617109	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	9418	0.4878536459782118	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	6642	0.34405647872024664	TruSeq Adapter, Index 15 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	6369	0.3299150426030188	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	5945	0.30795178650886273	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	5197	0.26920528755030443	No Hit
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	3783	0.19595990048158585	TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	2441	0.12644412293829	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36187572423059966	0.0
2	0.0	0.0	0.0	1.8208264503907543	0.0
3	5.180013229753789E-5	0.0	0.0	2.520646237730491	0.0
4	5.180013229753789E-5	0.0	0.0	3.430670961933637	0.0
5	5.180013229753789E-5	0.0	0.0	5.908530290386362	0.0
6	5.180013229753789E-5	0.0	0.0	7.039948780029184	0.0
7	5.180013229753789E-5	0.0	0.0	8.28449875860983	0.0
8	5.180013229753789E-5	0.0	0.0	10.08248135065737	0.0
9	5.180013229753789E-5	0.0	0.0	10.700974930289972	0.0
10	5.180013229753789E-5	0.0	0.0	12.448037992289033	0.0
11	5.180013229753789E-5	0.0	0.0	14.772154528082664	0.0
12	5.180013229753789E-5	0.0	0.0	16.911189191177193	0.0
13	5.180013229753789E-5	0.0	0.0	17.58914932268737	0.0
14	5.180013229753789E-5	0.0	0.0	17.843384372003687	0.0
15	5.180013229753789E-5	0.0	0.0	18.364493702916917	0.0
16	5.180013229753789E-5	0.0	0.0	19.397750941855904	0.0
17	5.180013229753789E-5	0.0	0.0	20.721347922322593	0.0
18	5.180013229753789E-5	0.0	0.0	22.124147305072217	0.0
19	5.180013229753789E-5	0.0	0.0	23.105293610919883	0.0
20	5.180013229753789E-5	0.0	0.0	23.933992127415895	0.0
21	5.180013229753789E-5	0.0	0.0	24.96999477336665	0.0
22	5.180013229753789E-5	0.0	0.0	26.058729953996302	0.0
23	5.180013229753789E-5	0.0	0.0	27.099291011589244	0.0
24	5.180013229753789E-5	0.0	0.0	27.93612214885597	0.0
25	5.180013229753789E-5	0.0	0.0	28.676553239916974	0.0
26	5.180013229753789E-5	0.0	0.0	29.36337119405003	0.0
27	5.180013229753789E-5	0.0	0.0	30.003206428189216	0.0
28	5.180013229753789E-5	0.0	0.0	30.655059293021434	0.0
29	5.180013229753789E-5	0.0	0.0	31.359437492003355	0.0
30	5.180013229753789E-5	0.0	0.0	32.14700670345512	0.0
31	1.0360026459507577E-4	0.0	0.0	32.87583456488148	0.0
32	1.0360026459507577E-4	0.0	0.0	33.52680682746464	0.0
33	1.0360026459507577E-4	0.0	0.0	34.138203788972476	0.0
34	1.0360026459507577E-4	0.0	0.0	34.769647401679464	0.0
35	1.0360026459507577E-4	0.0	0.0	35.44232391969529	0.0
36	1.0360026459507577E-4	0.0	0.0	36.076305738884855	0.0
37	1.0360026459507577E-4	0.0	0.0	36.69226111203488	0.0
38	1.0360026459507577E-4	0.0	0.0	37.316193705558725	0.0
39	1.0360026459507577E-4	0.0	0.0	37.93986729842108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	30	2.1662709E-6	45.000004	42
CCGTACA	30	2.1662709E-6	45.000004	15
CGGAAAT	25	3.8917948E-5	45.0	32
ACGTTCA	20	7.03448E-4	45.0	15
CGGCATA	25	3.8917948E-5	45.0	6
CACGATA	25	3.8917948E-5	45.0	10
TACGACG	55	1.8189894E-12	45.0	1
GTCGTGC	55	1.8189894E-12	45.0	22
CGTGTCG	20	7.03448E-4	45.0	2
TCGAGTA	25	3.8917948E-5	45.0	21
ACCCGGT	25	3.8917948E-5	45.0	29
CGGTCAA	20	7.03448E-4	45.0	45
TACGTTG	20	7.03448E-4	45.0	1
TAATCGC	25	3.8917948E-5	45.0	30
CGTCATA	70	0.0	41.785713	38
TCGCGTC	65	0.0	41.538464	18
CGCGTCG	60	3.6379788E-12	41.250004	19
CGTAAGG	180	0.0	41.25	2
TCGATAG	115	0.0	41.08696	1
CCGCTCG	55	6.184564E-11	40.909092	19
>>END_MODULE