##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934982.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1599204
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37264101390442	33.0	31.0	34.0	31.0	34.0
2	32.544065672672154	34.0	31.0	34.0	31.0	34.0
3	32.550728362360275	34.0	31.0	34.0	31.0	34.0
4	36.055651436589706	37.0	35.0	37.0	35.0	37.0
5	35.97123568975565	37.0	35.0	37.0	35.0	37.0
6	35.913165549861056	37.0	35.0	37.0	35.0	37.0
7	36.15211067506084	37.0	35.0	37.0	35.0	37.0
8	35.90483890735641	37.0	35.0	37.0	35.0	37.0
9	37.61128473915773	39.0	38.0	39.0	35.0	39.0
10	37.488551179211655	39.0	37.0	39.0	35.0	39.0
11	37.42572617377145	39.0	37.0	39.0	35.0	39.0
12	37.2546341805048	39.0	37.0	39.0	35.0	39.0
13	37.19084682129359	39.0	37.0	39.0	34.0	39.0
14	38.35280552074657	40.0	38.0	41.0	34.0	41.0
15	38.43098816661289	40.0	38.0	41.0	34.0	41.0
16	38.48522577482297	40.0	38.0	41.0	34.0	41.0
17	38.49460794245137	40.0	38.0	41.0	34.0	41.0
18	38.49702789637845	40.0	37.0	41.0	35.0	41.0
19	38.48026330599473	40.0	37.0	41.0	34.0	41.0
20	38.45110630038444	40.0	37.0	41.0	34.0	41.0
21	38.37613275104364	40.0	37.0	41.0	34.0	41.0
22	38.31845593182609	40.0	37.0	41.0	34.0	41.0
23	38.135524298338424	40.0	37.0	41.0	34.0	41.0
24	37.92557484848712	40.0	36.0	41.0	34.0	41.0
25	37.85469020837867	40.0	36.0	41.0	33.0	41.0
26	37.97970928036698	40.0	36.0	41.0	34.0	41.0
27	38.01902571529336	40.0	36.0	41.0	34.0	41.0
28	37.9739820560729	40.0	36.0	41.0	34.0	41.0
29	37.983266675170896	40.0	36.0	41.0	34.0	41.0
30	37.890397972991565	40.0	36.0	41.0	34.0	41.0
31	37.80327900630564	40.0	36.0	41.0	34.0	41.0
32	37.70753324779078	40.0	36.0	41.0	33.0	41.0
33	37.651100797646826	40.0	36.0	41.0	33.0	41.0
34	37.50239869334994	40.0	35.0	41.0	33.0	41.0
35	37.418990947996626	40.0	35.0	41.0	33.0	41.0
36	37.35432002421204	40.0	35.0	41.0	33.0	41.0
37	37.26293893712122	39.0	35.0	41.0	33.0	41.0
38	37.18688047303534	39.0	35.0	41.0	33.0	41.0
39	37.123531456899805	39.0	35.0	41.0	32.0	41.0
40	37.057302883184384	39.0	35.0	41.0	32.0	41.0
41	37.041394968997075	39.0	35.0	41.0	32.0	41.0
42	36.991792166602885	39.0	35.0	41.0	32.0	41.0
43	36.90475011318131	39.0	35.0	41.0	32.0	41.0
44	36.752576281700144	39.0	35.0	41.0	32.0	41.0
45	36.73705230852349	39.0	35.0	41.0	32.0	41.0
46	36.618171290216885	39.0	35.0	41.0	31.0	41.0
47	36.56119794597812	39.0	35.0	41.0	31.0	41.0
48	36.50160892544041	39.0	35.0	41.0	31.0	41.0
49	36.4208456206963	38.0	35.0	40.0	31.0	41.0
50	36.33621664277978	38.0	35.0	40.0	31.0	41.0
51	35.316989577314715	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	31.0
10	40.0
11	36.0
12	21.0
13	22.0
14	44.0
15	47.0
16	92.0
17	177.0
18	342.0
19	577.0
20	1097.0
21	1713.0
22	2574.0
23	3660.0
24	5327.0
25	7039.0
26	8815.0
27	10361.0
28	12374.0
29	15329.0
30	19215.0
31	24681.0
32	33199.0
33	48640.0
34	95369.0
35	139634.0
36	118207.0
37	190450.0
38	337214.0
39	522375.0
40	491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2268203431207	22.238563685433505	24.99043274028829	10.544183231157502
2	30.880738167238203	24.005192583310198	25.674898261885286	19.439170987566314
3	32.12985960515356	24.93490511529486	25.91851946343306	17.01671581611852
4	29.620548722989685	26.210164556867042	24.299276390004028	19.870010330139245
5	25.941655973846988	30.07940200249624	23.384133606469216	20.59480841718755
6	26.994617322117755	34.1449245999885	23.078544075677648	15.781914002216102
7	80.8833019427165	6.172695916218319	7.637612212075508	5.306389928989672
8	81.47928594475752	4.735605963967074	7.8971788464761214	5.887929244799288
9	75.48192725881125	8.113223828854856	9.748662459573639	6.656186452760249
10	41.570056102911195	26.99330416882399	16.608762859522614	14.827876868742198
11	30.53956843529656	24.24299839169987	23.854242485636604	21.363190687366966
12	27.98761133663998	21.796656336527423	29.215909915182802	20.999822411649795
13	24.386194631829337	24.918521964677424	30.837966888527042	19.857316514966193
14	19.354441334563948	27.70003076530574	30.367858009359654	22.577669890770657
15	18.89740145722497	25.872684160369786	34.240096948231745	20.989817434173503
16	21.75375999559781	25.48367812986961	30.80626361614903	21.956298258383548
17	21.06860663179932	25.309091272908272	27.566026598232618	26.05627549705979
18	21.661213953942084	25.023073979304705	30.65018596751884	22.66552609923437
19	22.928344351314777	26.44865820745821	27.384061070382515	23.238936370844495
20	24.775638380094097	26.6089254404066	28.141500396447235	20.47393578305207
21	23.46404836406112	26.2978957031123	29.254491609575762	20.983564323250818
22	23.39476389503778	23.719237820815856	27.806396182100595	25.07960210204577
23	22.070355001613304	25.488930743044662	27.68996325672022	24.750750998621815
24	22.491814677802207	24.15376649883317	30.107728594976003	23.246690228388623
25	21.065980325211793	26.955660441069433	27.050520133766547	24.927839099952227
26	20.926973669400525	28.390874460043875	27.125870120384892	23.55628175017071
27	22.444916345882078	27.56346282275432	26.908386922493943	23.083233908869662
28	19.82655120922659	26.750558402805396	28.643750265757213	24.779140122210798
29	20.601686839202504	26.50481114354391	28.074216922919152	24.819285094334433
30	22.843114449438595	27.994864945310294	26.551209226590228	22.610811378660884
31	23.406144556917067	28.363735958639424	23.91846193481257	24.31165754963094
32	23.29646499133319	28.71215929925138	23.918211810375663	24.073163899039773
33	25.179964532354848	27.44240259529116	23.880943269276464	23.49668960307753
34	22.608247603182583	26.976545831551196	26.34335581952021	24.07185074574601
35	22.61462577632372	26.902008749352802	25.21335614468198	25.270009329641496
36	26.147946103186335	27.611799370186667	24.471111878159384	21.769142648467614
37	23.666899282392993	27.325594483255422	26.240617207060513	22.766889027291075
38	22.139702001745867	28.19834117473443	24.833917373893513	24.82803944962619
39	24.22380134116723	26.0507102283386	25.95691356449834	23.768574865995834
40	24.082355972096117	26.006125547459863	26.98948977116115	22.92202870928287
41	22.38407357660436	25.075537579946023	26.41895593057546	26.121432912874155
42	24.245874822724307	24.57547629945898	25.834039934867597	25.344608942949115
43	24.454978851978858	24.824912894164846	26.52382060074887	24.196287653107422
44	21.88301179836969	25.588792924479932	26.125622497192353	26.402572779958028
45	22.921340867081373	26.04789632842339	25.772696916716065	25.258065887779168
46	23.678717662036863	26.548707982221153	25.90763905042759	23.864935305314393
47	21.73099867183924	26.078098854179956	28.353793512272357	23.83710896170845
48	21.60118408908432	24.435844332555444	28.145377325219297	25.817594253140935
49	23.581856973844488	24.083168876516066	28.75561842016403	23.579355729475417
50	21.811101022758823	23.843299541521905	28.976040580188645	25.369558855530627
51	21.620256077398505	23.47711736588953	26.00987741401347	28.89274914269849
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	728.0
1	1333.0
2	1938.0
3	2747.5
4	3557.0
5	2800.0
6	2043.0
7	2071.0
8	2099.0
9	2383.5
10	2668.0
11	2857.5
12	3047.0
13	2906.0
14	2765.0
15	3029.0
16	3293.0
17	3265.0
18	3237.0
19	3502.5
20	3768.0
21	4219.0
22	4670.0
23	5127.0
24	5584.0
25	7257.0
26	11445.0
27	13960.0
28	16740.5
29	19521.0
30	21570.5
31	23620.0
32	26783.0
33	29946.0
34	34850.5
35	39755.0
36	44516.5
37	49278.0
38	52141.0
39	55004.0
40	58430.5
41	61857.0
42	68554.5
43	75252.0
44	85848.0
45	96444.0
46	103952.0
47	111460.0
48	123577.5
49	135695.0
50	149284.5
51	162874.0
52	162173.0
53	161472.0
54	135809.5
55	110147.0
56	100760.0
57	91373.0
58	81646.0
59	71919.0
60	68609.5
61	65300.0
62	59555.5
63	53811.0
64	46712.5
65	39614.0
66	34013.5
67	28413.0
68	23283.0
69	18153.0
70	16566.5
71	14980.0
72	12281.0
73	9582.0
74	7692.0
75	4310.0
76	2818.0
77	2210.5
78	1603.0
79	1142.5
80	682.0
81	446.0
82	210.0
83	232.5
84	255.0
85	144.5
86	34.0
87	27.5
88	21.0
89	17.0
90	13.0
91	9.0
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1599204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.18716509451017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.10578560749323	12.683826221532671
2	12.694104972382245	4.8712777566428125
3	5.922083799714814	3.408839987059568
4	3.3221931049760802	2.5497387032407772
5	2.077135261301447	1.9927168591109694
6	1.3998140596861193	1.6115078078888452
7	0.9775019821831994	1.3128844338651993
8	0.7529819974816453	1.1558071919099497
9	0.5736294906590267	0.9905691366319075
>10	3.6519091244907256	14.246102262679782
>50	1.228925954236612	17.590101020714627
>100	1.2830446062983372	35.08607921254219
>500	0.007590027249100676	0.924504250978545
>1k	0.002640009477948061	0.8806882834641862
>5k	6.600023694870152E-4	0.6953568717379892
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	5689	0.3557394803914948	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	5293	0.33097716113766595	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	3764	0.2353670951298271	No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	2260	0.1413203068526592	No Hit
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1763	0.11024234556691954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.253110922684036E-5	0.0	0.0	0.24881128361359778	0.0
2	6.253110922684036E-5	0.0	0.0	1.2354271249946849	0.0
3	6.253110922684036E-5	0.0	0.0	1.7478070339994147	0.0
4	6.253110922684036E-5	0.0	0.0	2.3530456402060023	0.0
5	6.253110922684036E-5	0.0	0.0	3.9672236937876595	0.0
6	6.253110922684036E-5	0.0	0.0	4.801888939747524	0.0
7	6.253110922684036E-5	0.0	0.0	5.643995387705384	0.0
8	6.253110922684036E-5	0.0	0.0	6.934449888819688	0.0
9	6.253110922684036E-5	0.0	0.0	7.38185997533773	0.0
10	6.253110922684036E-5	0.0	0.0	8.413998464235958	0.0
11	6.253110922684036E-5	0.0	0.0	10.105214844385081	0.0
12	1.250622184536807E-4	0.0	0.0	11.378660883789685	0.0
13	1.250622184536807E-4	0.0	0.0	11.900858176943029	0.0
14	1.250622184536807E-4	0.0	0.0	12.100707602032012	0.0
15	1.250622184536807E-4	0.0	0.0	12.491714628027443	0.0
16	1.250622184536807E-4	0.0	0.0	13.315124274326477	0.0
17	1.250622184536807E-4	0.0	0.0	14.299739120212305	0.0
18	5.002488738147228E-4	0.0	0.0	15.422422655270998	0.0
19	5.002488738147228E-4	0.0	0.0	16.077561086640603	0.0
20	5.002488738147228E-4	0.0	0.0	16.70581114104267	0.0
21	5.002488738147228E-4	0.0	0.0	17.473317975692908	0.0
22	6.253110922684036E-4	0.0	0.0	18.26133501416955	0.0
23	6.253110922684036E-4	0.0	0.0	19.038409108531493	0.0
24	6.253110922684036E-4	0.0	0.0	19.69317235324574	0.0
25	6.253110922684036E-4	0.0	0.0	20.257265489581066	0.0
26	6.253110922684036E-4	0.0	0.0	20.756826521194295	0.0
27	6.253110922684036E-4	0.0	0.0	21.249634193011023	0.0
28	6.253110922684036E-4	0.0	0.0	21.782774430279066	0.0
29	6.253110922684036E-4	0.0	0.0	22.33798814910418	0.0
30	6.253110922684036E-4	0.0	0.0	22.94335181752922	0.0
31	6.878422014952439E-4	0.0	0.0	23.470113881656125	0.0
32	6.878422014952439E-4	0.0	0.0	23.982243666223948	0.0
33	6.878422014952439E-4	0.0	0.0	24.5030652749743	0.0
34	8.129044199489246E-4	0.0	0.0	25.02057273493563	0.0
35	8.129044199489246E-4	0.0	0.0	25.587104584530802	0.0
36	8.129044199489246E-4	0.0	0.0	26.114679553077657	0.0
37	8.75435529175765E-4	0.0	0.0	26.638315061743217	0.0
38	9.379666384026053E-4	0.0	0.0	27.177583347715487	0.0
39	0.001063028856856286	0.0	0.0	27.719477940275286	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.034115E-4	45.000004	4
ATAATCG	30	2.1660362E-6	45.000004	2
TTCGTAA	20	7.034115E-4	45.000004	18
TATCTCG	20	7.034115E-4	45.000004	1
CGTAGAC	20	7.034115E-4	45.000004	33
CATACCG	30	2.1660362E-6	45.000004	28
TCCGTAG	20	7.034115E-4	45.000004	29
TCGTAAG	20	7.034115E-4	45.000004	19
CGTAAGC	20	7.034115E-4	45.000004	20
ATACGTT	20	7.034115E-4	45.000004	26
CGGTACG	30	2.1660362E-6	45.000004	14
TATCGTG	20	7.034115E-4	45.000004	17
CGATACC	30	2.1660362E-6	45.000004	41
CCGTTGA	20	7.034115E-4	45.000004	11
ATCGCAC	20	7.034115E-4	45.000004	11
TAGTACG	60	0.0	45.000004	1
TCTATCG	20	7.034115E-4	45.000004	12
ACGTTGC	25	3.8914928E-5	45.0	37
TAAGTCG	35	1.21232E-7	45.0	1
CGCGACT	25	3.8914928E-5	45.0	16
>>END_MODULE