##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934981.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	965107
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1814182261656	33.0	31.0	34.0	30.0	34.0
2	32.36555117722698	34.0	31.0	34.0	30.0	34.0
3	32.37115262867226	34.0	31.0	34.0	30.0	34.0
4	35.917706534094144	37.0	35.0	37.0	35.0	37.0
5	35.840293356073474	37.0	35.0	37.0	35.0	37.0
6	35.77785675577941	37.0	35.0	37.0	35.0	37.0
7	36.09756949229464	37.0	35.0	37.0	35.0	37.0
8	35.86064343124649	37.0	35.0	37.0	35.0	37.0
9	37.57723858598062	39.0	38.0	39.0	35.0	39.0
10	37.37240533951158	39.0	37.0	39.0	34.0	39.0
11	37.30479832806103	39.0	37.0	39.0	34.0	39.0
12	37.22190285636722	39.0	37.0	39.0	34.0	39.0
13	37.14449796758287	39.0	37.0	39.0	33.0	39.0
14	38.2018014582839	40.0	37.0	41.0	33.0	41.0
15	38.28710806159317	40.0	37.0	41.0	34.0	41.0
16	38.35587349381986	40.0	37.0	41.0	34.0	41.0
17	38.37021594496776	40.0	37.0	41.0	34.0	41.0
18	38.38609294098996	40.0	37.0	41.0	34.0	41.0
19	38.39071004562189	40.0	37.0	41.0	34.0	41.0
20	38.344552469311694	40.0	37.0	41.0	34.0	41.0
21	38.2723480401655	40.0	37.0	41.0	34.0	41.0
22	38.19252165822028	40.0	37.0	41.0	34.0	41.0
23	37.98227243196868	40.0	36.0	41.0	34.0	41.0
24	37.74874288550389	39.0	36.0	41.0	33.0	41.0
25	37.72305765060247	39.0	36.0	41.0	33.0	41.0
26	37.9062653156593	40.0	36.0	41.0	34.0	41.0
27	37.97035665475434	40.0	37.0	41.0	34.0	41.0
28	37.98856603464694	40.0	37.0	41.0	34.0	41.0
29	38.05630878234227	40.0	37.0	41.0	34.0	41.0
30	37.953670422036105	40.0	36.0	41.0	34.0	41.0
31	37.83221238681307	40.0	36.0	41.0	34.0	41.0
32	37.778285723759126	40.0	36.0	41.0	34.0	41.0
33	37.68922927716823	40.0	36.0	41.0	33.0	41.0
34	37.58217689852006	40.0	36.0	41.0	33.0	41.0
35	37.55016179553148	40.0	36.0	41.0	33.0	41.0
36	37.445597223934755	40.0	36.0	41.0	33.0	41.0
37	37.36307994864818	40.0	35.0	41.0	33.0	41.0
38	37.3201458491131	40.0	35.0	41.0	33.0	41.0
39	37.26060944537756	39.0	35.0	41.0	33.0	41.0
40	37.21301472271986	39.0	35.0	41.0	33.0	41.0
41	37.14307636355347	39.0	35.0	41.0	32.0	41.0
42	37.171033885361936	39.0	35.0	41.0	33.0	41.0
43	37.09678512330757	39.0	35.0	41.0	32.0	41.0
44	36.97259889317972	39.0	35.0	41.0	32.0	41.0
45	36.987938124995466	39.0	35.0	41.0	32.0	41.0
46	36.865677070003635	39.0	35.0	41.0	32.0	41.0
47	36.758212301848395	39.0	35.0	41.0	31.0	41.0
48	36.731822481859524	39.0	35.0	41.0	31.0	41.0
49	36.71101857099783	39.0	35.0	41.0	31.0	41.0
50	36.62648079435752	39.0	35.0	41.0	31.0	41.0
51	35.53963861001941	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	9.0
11	14.0
12	10.0
13	8.0
14	17.0
15	28.0
16	44.0
17	88.0
18	182.0
19	372.0
20	639.0
21	1048.0
22	1679.0
23	2491.0
24	3449.0
25	4223.0
26	5214.0
27	6026.0
28	7341.0
29	9383.0
30	11725.0
31	15589.0
32	21059.0
33	29609.0
34	54715.0
35	81342.0
36	69876.0
37	108784.0
38	213953.0
39	315943.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.32507690857076	23.698926647511623	25.519346559500654	12.456649884416962
2	30.578371102893254	25.455312208905333	25.45997490433703	18.50634178386438
3	30.26752474078004	25.141771844987137	27.756922289445622	16.8337811247872
4	28.775462202636596	25.78035388822172	26.839200213033376	18.604983696108306
5	24.248295784819714	28.63454518514527	25.429097499033787	21.688061531001228
6	25.62575963079741	32.36832807139519	24.891644138940034	17.11426815886736
7	78.13485965804828	5.578137968121669	10.136492637603913	6.150509736226139
8	77.05466854970486	4.69647406971455	9.937654581305493	8.311202799275106
9	70.77526118865578	7.372861247509343	13.22703078518755	8.624846778647342
10	35.73261824854653	30.772028386489787	19.475146279117237	14.02020708584644
11	25.103330511539134	23.604118507067092	30.21872186192826	21.073829119465508
12	23.560496400917206	21.03808178782249	35.046373096454595	20.355048714805715
13	22.543199873174686	25.153791237655515	33.705174659390096	18.597834229779703
14	16.827771428453012	30.648518765276805	30.95376989287198	21.569939913398205
15	14.864051343529786	27.379554805840183	37.87300268260411	19.88339116802593
16	16.557335093414512	28.58512061356927	32.816879371924564	22.04066492109165
17	17.31953037331612	27.39789474120486	28.670499747696372	26.612075137782647
18	17.039250570144034	26.23170280601011	33.44385648430692	23.285190139538933
19	18.941526690822883	27.616626964678524	29.962066382276788	23.479779962221805
20	20.92710963654807	28.86519318583328	30.28793698522547	19.919760192393174
21	19.8168700465337	28.5363177347175	31.915528537250275	19.731283681498528
22	18.325947278384678	26.34485088181932	29.238312435823175	26.090889403972824
23	17.00288154577679	26.96623275968364	29.97688339220418	26.054002302335387
24	20.43089522716134	25.117214982380194	30.07666507444252	24.375224716015946
25	17.316421909694988	28.47891477318059	28.58242661176429	25.62223670536013
26	16.547284394372852	28.229615990765787	30.48004003701144	24.74305957784992
27	19.44520141290033	27.45218923912064	29.258206602998428	23.84440274498061
28	17.244512784592796	25.895574273111688	31.276117570383388	25.58379537191213
29	17.829629253543906	22.294833629846224	33.238076192587975	26.637460924021894
30	19.56726041775679	25.89640319674399	29.404304393191637	25.132031992307592
31	21.54807705259624	29.238519666731257	24.92842762512343	24.284975655549072
32	21.825041161239117	26.991411315014812	26.344332804549133	24.83921471919694
33	20.71034610670112	24.420608284884473	29.14992845352899	25.719117154885417
34	19.275893761002667	25.379672927457786	30.78612008823892	24.55831322330063
35	19.99166931749537	22.682666274309483	29.457977198383183	27.86768720981197
36	21.357839078982952	22.741934314019066	32.68404435984818	23.216182247149796
37	20.98679213807381	25.636639253471376	31.16328034093629	22.213288267518525
38	20.36136925750202	25.120427061455363	28.79380213800128	25.724401543041342
39	19.910952878799968	24.46205446649957	32.42365872385135	23.20333393084912
40	21.829600241216777	23.712914733806716	32.879152259801245	21.578332765175258
41	20.067204983488875	24.673844454552707	28.85876902768294	26.400181534275475
42	21.702256848204396	21.256503164934042	32.330508430671415	24.710731556190144
43	23.697372415701057	20.948972497350034	31.065363736870626	24.288291350078282
44	19.89717202341295	22.680283118866612	29.58107235778002	27.841472499940423
45	20.23526924993809	22.371923527650303	28.215524289016656	29.17728293339495
46	23.13028503575251	23.27617559503765	29.83482660471844	23.7587127644914
47	18.1634782464535	22.976623317414546	34.57751316693382	24.28238526919813
48	19.39090691498456	20.704232794912897	32.55359250321467	27.351267786887878
49	21.42933374226899	18.601046308854873	35.7371773285242	24.23244262035194
50	20.22283539545356	19.530787777935505	32.82226737553452	27.42410945107641
51	18.223886056157504	19.860699383591665	29.423058790372465	32.492355769878365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1100.0
1	1237.0
2	1374.0
3	1728.5
4	2083.0
5	1602.5
6	1122.0
7	1194.5
8	1267.0
9	1532.0
10	1797.0
11	1872.0
12	1947.0
13	2251.0
14	2555.0
15	2480.5
16	2406.0
17	2266.5
18	2127.0
19	2472.5
20	2818.0
21	3285.0
22	3752.0
23	3943.0
24	4134.0
25	5316.5
26	7208.5
27	7918.0
28	9986.5
29	12055.0
30	13445.5
31	14836.0
32	16764.0
33	18692.0
34	20591.0
35	22490.0
36	25077.5
37	27665.0
38	30802.5
39	33940.0
40	38121.5
41	42303.0
42	48567.5
43	54832.0
44	63769.0
45	72706.0
46	91043.0
47	109380.0
48	126253.5
49	143127.0
50	137753.0
51	132379.0
52	104815.5
53	77252.0
54	62610.0
55	47968.0
56	39893.0
57	31818.0
58	28040.0
59	24262.0
60	21156.0
61	18050.0
62	16307.5
63	14565.0
64	12012.0
65	9459.0
66	7205.0
67	4951.0
68	4320.5
69	3690.0
70	2865.5
71	2041.0
72	1978.0
73	1915.0
74	1442.0
75	679.5
76	390.0
77	308.5
78	227.0
79	208.5
80	190.0
81	105.5
82	21.0
83	14.5
84	8.0
85	6.5
86	5.0
87	13.0
88	21.0
89	11.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	965107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.68822132589392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.3614218252544	13.525083187435492
2	13.01652345505123	5.646104831736809
3	6.655747591608894	4.330539805682974
4	4.040454461338391	3.505210824588101
5	2.6121337705789776	2.832626767457934
6	1.780350687839859	2.3167583853347
7	1.3071786749715266	1.9845266290689854
8	0.9284590014401748	1.6109299452202377
9	0.744905970696264	1.454011700349638
>10	4.700875327777921	20.58259434427946
>50	1.3432779392401506	20.998569861040515
>100	0.4913137903242016	15.301679857352676
>500	0.01012521059549385	1.464194529878918
>1k	0.005785834625996486	2.0516709524513908
>5k	0.0014464586564991214	2.3954983781221433
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	9659	1.0008216705505193	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	7522	0.7793954452718713	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	5727	0.5934057052741302	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	2921	0.3026607412442351	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	2097	0.21728160711713831	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1800	0.1865078172679299	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	1702	0.17635350277223147	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	1577	0.16340157101751412	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1498	0.15521595014853273	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1487	0.15407618015411761	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	1442	0.1494134847224194	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	1404	0.1454760974689853	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1271	0.13169524208196606	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	1227	0.12713616210430553	No Hit
GGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1194	0.12371685212106015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6201384924158668	0.0
2	0.0	0.0	0.0	3.028886952431181	0.0
3	0.0	0.0	0.0	4.1665846377655535	0.0
4	0.0	0.0	0.0	5.411420702574947	0.0
5	0.0	0.0	0.0	8.787730272394667	0.0
6	0.0	0.0	0.0	10.364343021032902	0.0
7	0.0	0.0	0.0	12.02187943927461	0.0
8	0.0	0.0	0.0	14.513831108882227	0.0
9	0.0	0.0	0.0	15.348039129340062	0.0
10	0.0	0.0	0.0	17.402526352000347	0.0
11	0.0	0.0	0.0	20.476900488754097	0.0
12	0.0	0.0	0.0	22.975172701058018	0.0
13	0.0	0.0	0.0	23.860463140356458	0.0
14	0.0	0.0	0.0	24.20446644776175	0.0
15	0.0	0.0	0.0	24.86770896905732	0.0
16	0.0	0.0	0.0	26.20383024887396	0.0
17	0.0	0.0	0.0	27.880639141566686	0.0
18	0.0	0.0	0.0	29.72893161069187	0.0
19	0.0	0.0	0.0	30.895434392248735	0.0
20	0.0	0.0	0.0	31.84797126121767	0.0
21	0.0	0.0	0.0	33.06897577159838	0.0
22	0.0	0.0	0.0	34.304590060998414	0.0
23	0.0	0.0	0.0	35.43928289816569	0.0
24	0.0	0.0	0.0	36.351098893697795	0.0
25	0.0	0.0	0.0	37.08293484556635	0.0
26	1.0361545403773882E-4	0.0	0.0	37.78762354847701	0.0
27	1.0361545403773882E-4	0.0	0.0	38.49324479047401	0.0
28	1.0361545403773882E-4	0.0	0.0	39.18891894888339	0.0
29	1.0361545403773882E-4	0.0	0.0	39.90210411902515	0.0
30	1.0361545403773882E-4	0.0	0.0	40.756309922112266	0.0
31	3.1084636211321646E-4	0.0	0.0	41.485244641267755	0.0
32	3.1084636211321646E-4	0.0	0.0	42.18703211146536	0.0
33	3.1084636211321646E-4	0.0	0.0	42.800850061184924	0.0
34	3.1084636211321646E-4	0.0	0.0	43.503673685922905	0.0
35	3.1084636211321646E-4	0.0	0.0	44.229292710549196	0.0
36	3.1084636211321646E-4	0.0	0.0	44.86497352107072	0.0
37	3.1084636211321646E-4	0.0	0.0	45.48480116712447	0.0
38	3.1084636211321646E-4	0.0	0.0	46.069606789713475	0.0
39	3.1084636211321646E-4	0.0	0.0	46.72694323012889	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.032723E-4	45.000004	20
TCGCCCA	20	7.032723E-4	45.000004	22
CGGGTTA	90	0.0	45.000004	6
TCGCATA	20	7.032723E-4	45.000004	35
TCCGCGA	20	7.032723E-4	45.000004	33
TCCGCAC	20	7.032723E-4	45.000004	23
CGTATCA	20	7.032723E-4	45.000004	30
CGAAACT	35	1.2116652E-7	45.000004	23
GGCACGC	20	7.032723E-4	45.000004	9
CGCATCG	20	7.032723E-4	45.000004	21
CGCATAT	20	7.032723E-4	45.000004	36
GGCCGTA	20	7.032723E-4	45.000004	33
CGGCACG	20	7.032723E-4	45.000004	31
GACCGAC	45	3.8380676E-10	45.000004	9
GTCTCGC	45	3.8380676E-10	45.000004	31
CGCGCAT	30	2.1651376E-6	45.000004	25
TTCGTGC	30	2.1651376E-6	45.000004	42
AAGCGTA	20	7.032723E-4	45.000004	31
CGCGATT	20	7.032723E-4	45.000004	21
TTCCGCG	40	6.8139343E-9	45.000004	1
>>END_MODULE