##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934980.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	948214
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21300255005727	33.0	31.0	34.0	30.0	34.0
2	32.39814746460187	34.0	31.0	34.0	30.0	34.0
3	32.40347853965455	34.0	31.0	34.0	30.0	34.0
4	35.936536478052425	37.0	35.0	37.0	35.0	37.0
5	35.84313456666955	37.0	35.0	37.0	35.0	37.0
6	35.77873771110741	37.0	35.0	37.0	35.0	37.0
7	36.058151429951465	37.0	35.0	37.0	35.0	37.0
8	35.78246682710865	37.0	35.0	37.0	35.0	37.0
9	37.449203449854146	39.0	37.0	39.0	35.0	39.0
10	37.33802706983867	39.0	37.0	39.0	34.0	39.0
11	37.288352629258796	39.0	37.0	39.0	34.0	39.0
12	37.142583847106245	39.0	37.0	39.0	34.0	39.0
13	37.03567127251865	39.0	37.0	39.0	33.0	39.0
14	38.149564338851775	40.0	37.0	41.0	33.0	41.0
15	38.19371049151352	40.0	37.0	41.0	33.0	41.0
16	38.256069832337424	40.0	37.0	41.0	34.0	41.0
17	38.266101323119045	40.0	37.0	41.0	34.0	41.0
18	38.24474327525221	40.0	37.0	41.0	34.0	41.0
19	38.21474266357595	40.0	37.0	41.0	34.0	41.0
20	38.18176276663285	40.0	37.0	41.0	34.0	41.0
21	38.14112004252205	40.0	37.0	41.0	34.0	41.0
22	38.07694149211043	40.0	36.0	41.0	34.0	41.0
23	37.86059897871156	40.0	36.0	41.0	33.0	41.0
24	37.62213171288338	39.0	35.0	41.0	33.0	41.0
25	37.59278074358742	39.0	35.0	41.0	33.0	41.0
26	37.76643563583748	39.0	36.0	41.0	33.0	41.0
27	37.79294969279087	40.0	36.0	41.0	33.0	41.0
28	37.73592986393367	40.0	36.0	41.0	33.0	41.0
29	37.77359224816339	40.0	36.0	41.0	33.0	41.0
30	37.70727388543093	40.0	36.0	41.0	33.0	41.0
31	37.54875059849359	40.0	36.0	41.0	33.0	41.0
32	37.44326491699131	39.0	35.0	41.0	33.0	41.0
33	37.38136116952502	39.0	35.0	41.0	33.0	41.0
34	37.272256051903895	39.0	35.0	41.0	33.0	41.0
35	37.15932690299869	39.0	35.0	41.0	32.0	41.0
36	37.13214316599417	39.0	35.0	41.0	32.0	41.0
37	37.04517862001616	39.0	35.0	41.0	32.0	41.0
38	36.93644050815533	39.0	35.0	41.0	31.0	41.0
39	36.92352992046099	39.0	35.0	41.0	31.0	41.0
40	36.865924780692964	39.0	35.0	41.0	31.0	41.0
41	36.77839917993196	39.0	35.0	41.0	31.0	41.0
42	36.75135570662319	39.0	35.0	41.0	31.0	41.0
43	36.669241331598144	39.0	35.0	41.0	31.0	41.0
44	36.5388530437222	39.0	35.0	41.0	31.0	41.0
45	36.53305161071235	39.0	35.0	41.0	31.0	41.0
46	36.391810287551124	39.0	35.0	41.0	31.0	41.0
47	36.2946908609238	39.0	35.0	40.0	31.0	41.0
48	36.21985859732086	38.0	35.0	40.0	31.0	41.0
49	36.182565328079946	38.0	35.0	40.0	31.0	41.0
50	36.102423081709404	38.0	35.0	40.0	31.0	41.0
51	35.03938984237735	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	11.0
11	17.0
12	9.0
13	11.0
14	19.0
15	26.0
16	58.0
17	106.0
18	258.0
19	455.0
20	762.0
21	1268.0
22	1947.0
23	2847.0
24	3899.0
25	4981.0
26	6276.0
27	7534.0
28	8758.0
29	10550.0
30	13094.0
31	16954.0
32	22066.0
33	31785.0
34	60946.0
35	81533.0
36	70919.0
37	111515.0
38	202920.0
39	286470.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.3329375014501	23.96537068636405	25.586629178645325	14.115062633540528
2	30.472340631967043	28.145228819654633	23.406214209028764	17.976216339349556
3	30.64318814107364	25.57650488180938	25.795020955185223	17.985286021931756
4	29.506103052686417	25.379924784911424	25.965763002866442	19.148209159535718
5	26.051819525971986	28.60577886426482	24.757175068075348	20.585226541687845
6	26.411864832200326	32.94393459704244	24.07273041739523	16.571470153362007
7	77.2367841014792	6.533229840521232	10.130519060043408	6.099466997956158
8	76.03452385221058	5.644084563189322	10.463566241375892	7.857825343224209
9	69.2245632315068	8.838616599206508	12.50371751524445	9.433102654042232
10	37.09848198824316	27.811232485493782	20.03176498132278	15.058520544940276
11	30.409169238167756	24.56787180952823	25.106779693191623	19.916179259112393
12	27.85151874998682	21.376187231996152	30.514103356415323	20.258190661601706
13	24.460933924198546	24.699698591246282	30.989523462003305	19.84984402255187
14	19.272653641477557	28.70153783850481	29.135089758219134	22.8907187617985
15	16.80127059925291	26.346795132744294	36.18318227741839	20.668751990584404
16	19.345316563560548	26.75672369317475	31.64918467772043	22.248775065544276
17	19.486107566435425	25.92990611823913	28.753319398363658	25.830666916961782
18	20.14619062785405	26.571111584515734	31.20835591965527	22.07434186797495
19	21.230439542128675	27.645552586230533	28.282539595492157	22.841468276148632
20	22.613038828787595	28.44642665052404	28.14723258673675	20.793301933951618
21	22.000624331638218	27.77316091093361	29.58435543031425	20.64185932711392
22	19.925248941694594	26.31905877787082	27.973221234868923	25.782471045565664
23	19.15232215512532	27.532392476803757	29.396001324595506	23.919284043475418
24	22.45790507206179	23.87013901925093	29.970238785759335	23.701717122927946
25	19.55729402856317	27.492528058012223	26.85322089739236	26.096957016032245
26	19.044435116967268	28.05579753093711	27.344354755361135	25.555412596734495
27	22.176323066312033	27.373356647339104	28.373869189866426	22.076451096482437
28	18.267606257659136	25.471043456434938	30.39651386712282	25.8648364187831
29	19.432005855218335	24.462515845579162	28.540709164808785	27.564769134393714
30	24.28502426667398	24.441529021929647	27.461522398952138	23.811924312444237
31	22.10903867692314	26.755774540346376	25.762327913319144	25.372858869411335
32	22.312368305045062	26.834765147951835	24.581898179103028	26.27096836790007
33	24.97990959846617	25.050146907765548	25.411035905396883	24.5589075883714
34	20.093987222293702	24.191163598090725	27.940528193002844	27.77432098661273
35	20.62023973491216	25.0214614000637	26.01680633274767	28.34149253227647
36	22.58561885819024	25.21972887976765	28.201017913677713	23.9936343483644
37	21.00633401320799	26.035789389315074	29.459489102670915	23.498387494806025
38	20.390228366170508	27.119932842164324	26.90637345578108	25.583465335884092
39	23.137393035749316	22.98531766035937	28.324302319940436	25.55298698395088
40	24.09055339828351	22.57581094563042	28.186569698401414	25.147065957684656
41	19.609813818399644	25.409032138314767	27.298795419599376	27.682358623686216
42	20.804902690742807	24.12377374727646	29.996076834976073	25.075246727004664
43	22.751931526005734	22.52339661721932	29.75077355955512	24.973898297219826
44	20.953075993393895	22.67251907269878	28.45876563729285	27.915639296614476
45	22.362778866374047	21.92015726407752	26.083879799285814	29.63318407026262
46	23.749596610047945	24.322357611256532	27.01710795242424	24.910937826271283
47	19.57817539078731	23.51441763146294	32.05341832118066	24.853988656569086
48	19.63797201897462	22.496714876599587	31.158050819751658	26.707262284674133
49	21.80952822885973	20.209256560227963	32.94340728991557	25.037807920996734
50	20.33971234341615	21.412571423750336	30.713636373223768	27.53407985960975
51	19.370732767075786	20.538823514523095	27.96225324662998	32.12819047177114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1208.0
1	1511.5
2	1815.0
3	2328.5
4	2842.0
5	2239.0
6	1636.0
7	1725.0
8	1814.0
9	1991.5
10	2169.0
11	2280.0
12	2391.0
13	2417.0
14	2443.0
15	2532.5
16	2622.0
17	2639.0
18	2656.0
19	2874.5
20	3093.0
21	3149.5
22	3206.0
23	3927.5
24	4649.0
25	6091.0
26	8319.5
27	9106.0
28	9515.5
29	9925.0
30	11382.0
31	12839.0
32	14961.5
33	17084.0
34	19651.0
35	22218.0
36	22506.0
37	22794.0
38	25171.5
39	27549.0
40	30858.5
41	34168.0
42	40192.0
43	46216.0
44	50501.0
45	54786.0
46	60244.5
47	65703.0
48	78922.5
49	92142.0
50	103138.0
51	114134.0
52	112906.0
53	111678.0
54	91696.5
55	71715.0
56	60826.0
57	49937.0
58	43680.0
59	37423.0
60	34810.5
61	32198.0
62	28851.0
63	25504.0
64	21853.0
65	18202.0
66	14518.0
67	10834.0
68	9072.0
69	7310.0
70	6543.5
71	5777.0
72	4865.0
73	3953.0
74	3130.0
75	1807.0
76	1307.0
77	982.5
78	658.0
79	474.5
80	291.0
81	215.5
82	140.0
83	117.0
84	94.0
85	77.0
86	60.0
87	48.0
88	36.0
89	41.5
90	47.0
91	24.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	948214.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.995934494020347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.11677512769013	14.974210952982153
2	12.904744137663688	5.9351330170361445
3	6.098153774311454	4.206982341855873
4	3.5046005400200158	3.2236625778403445
5	2.2686068063304834	2.608436675553225
6	1.5563225032276407	2.147345420147556
7	1.1105437983351645	1.787659470747919
8	0.8143483214714115	1.4981360524697598
9	0.5954121461144839	1.2322852838093468
>10	4.1637433175264755	20.327264632036528
>50	1.3976332893274588	22.889746338207306
>100	0.4556996986310599	14.919565874033266
>500	0.006939589319254719	1.133885729767797
>1k	0.00601431074335409	2.316497267776695
>5k	4.626392879503146E-4	0.7991883657361316
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	7512	0.7922262274127992	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	3738	0.3942148080496597	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	3300	0.3480227037356546	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1859	0.19605278977108542	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1576	0.16620720639011868	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1474	0.15545014100192572	No Hit
ATTACGGGGGCAGTTACCAAACATTGTTATGCAAGAAATCATAAAATGAAG	1470	0.15502829530042797	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	1395	0.1471186883973449	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	1345	0.14184561712862287	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	1276	0.13456877877778645	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1167	0.12307348341197241	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	1134	0.11959325637461585	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	1023	0.10788703815805294	No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1017	0.10725426960580628	No Hit
GGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	991	0.10451227254607083	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	980	0.10335219686695198	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	955	0.10071566123259096	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1092285074888158E-4	0.0	0.0	0.37038052591503606	0.0
2	2.1092285074888158E-4	0.0	0.0	2.00007593222627	0.0
3	2.1092285074888158E-4	0.0	0.0	2.9060950376180905	0.0
4	2.1092285074888158E-4	0.0	0.0	3.9408825433921035	0.0
5	2.1092285074888158E-4	0.0	0.0	6.676657379030472	0.0
6	2.1092285074888158E-4	0.0	0.0	8.04902690742807	0.0
7	2.1092285074888158E-4	0.0	0.0	9.521795712782136	0.0
8	2.1092285074888158E-4	0.0	0.0	11.429487436380395	0.0
9	2.1092285074888158E-4	0.0	0.0	12.126165612403952	0.0
10	2.1092285074888158E-4	0.0	0.0	13.749533333192717	0.0
11	2.1092285074888158E-4	0.0	0.0	16.233993592163795	0.0
12	2.1092285074888158E-4	0.0	0.0	18.277730554495083	0.0
13	2.1092285074888158E-4	0.0	0.0	19.012374843653436	0.0
14	2.1092285074888158E-4	0.0	0.0	19.306506759022753	0.0
15	2.1092285074888158E-4	0.0	0.0	19.92767455447821	0.0
16	2.1092285074888158E-4	0.0	0.0	21.11538112704516	0.0
17	2.1092285074888158E-4	0.0	0.0	22.426372105874833	0.0
18	2.1092285074888158E-4	0.0	0.0	23.85832733960899	0.0
19	2.1092285074888158E-4	0.0	0.0	24.755804069545484	0.0
20	2.1092285074888158E-4	0.0	0.0	25.634825050041446	0.0
21	2.1092285074888158E-4	0.0	0.0	26.693974145076954	0.0
22	2.1092285074888158E-4	0.0	0.0	27.756181621448324	0.0
23	3.1638427612332235E-4	0.0	0.0	28.720626356497583	0.0
24	3.1638427612332235E-4	0.0	0.0	29.480265003469682	0.0
25	3.1638427612332235E-4	0.0	0.0	30.15047236172425	0.0
26	3.1638427612332235E-4	0.0	0.0	30.77670230559768	0.0
27	3.1638427612332235E-4	0.0	0.0	31.40904901214283	0.0
28	4.2184570149776315E-4	0.0	0.0	32.04223941009097	0.0
29	4.2184570149776315E-4	0.0	0.0	32.69789309164387	0.0
30	4.2184570149776315E-4	0.0	0.0	33.4087030986676	0.0
31	4.2184570149776315E-4	0.0	0.0	34.086398218123755	0.0
32	4.2184570149776315E-4	0.0	0.0	34.7439502053334	0.0
33	4.2184570149776315E-4	0.0	0.0	35.34961517125881	0.0
34	4.2184570149776315E-4	0.0	0.0	35.9549637529081	0.0
35	4.2184570149776315E-4	0.0	0.0	36.62031988559544	0.0
36	4.2184570149776315E-4	0.0	0.0	37.22999238568509	0.0
37	4.2184570149776315E-4	0.0	0.0	37.833653584528385	0.0
38	4.2184570149776315E-4	0.0	0.0	38.4479663873345	0.0
39	4.2184570149776315E-4	0.0	0.0	39.04392890212547	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	25	3.890287E-5	45.0	38
AACCGTA	25	3.890287E-5	45.0	24
GGTAACG	25	3.890287E-5	45.0	29
CGACGGA	20	7.032662E-4	45.0	3
ACGTTAT	20	7.032662E-4	45.0	11
ACGTTAG	35	1.2116288E-7	45.0	1
TTAGCGA	25	3.890287E-5	45.0	21
TAGACCG	45	3.8380676E-10	45.0	1
TTCACGT	20	7.032662E-4	45.0	19
GGTCCAC	35	1.2116288E-7	45.0	35
GAATCGT	25	3.890287E-5	45.0	10
GAATCGG	20	7.032662E-4	45.0	33
GATTTCG	20	7.032662E-4	45.0	22
TACGGTC	20	7.032662E-4	45.0	14
ATCGTAG	25	3.890287E-5	45.0	1
ATATCCG	20	7.032662E-4	45.0	1
TACGATT	25	3.890287E-5	45.0	40
TAACACG	25	3.890287E-5	45.0	1
ACGGATA	35	1.2116288E-7	45.0	32
ACTACGT	35	1.2116288E-7	45.0	17
>>END_MODULE