##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934979.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1255866
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2035957657903	33.0	31.0	34.0	30.0	34.0
2	32.374833780037044	34.0	31.0	34.0	30.0	34.0
3	32.384670020527665	34.0	31.0	34.0	30.0	34.0
4	35.91933534310189	37.0	35.0	37.0	35.0	37.0
5	35.8451745648023	37.0	35.0	37.0	35.0	37.0
6	35.79259491060352	37.0	35.0	37.0	35.0	37.0
7	36.10174572764929	37.0	35.0	37.0	35.0	37.0
8	35.88985449084536	37.0	35.0	37.0	35.0	37.0
9	37.58522644931864	39.0	38.0	39.0	35.0	39.0
10	37.37756655566756	39.0	37.0	39.0	34.0	39.0
11	37.31259545206256	39.0	37.0	39.0	34.0	39.0
12	37.24826534041052	39.0	37.0	39.0	34.0	39.0
13	37.181841056291034	39.0	37.0	39.0	34.0	39.0
14	38.33299173637952	40.0	38.0	41.0	34.0	41.0
15	38.40167501946864	40.0	38.0	41.0	34.0	41.0
16	38.45276088372486	40.0	38.0	41.0	34.0	41.0
17	38.478599627667286	40.0	38.0	41.0	34.0	41.0
18	38.47880904491403	40.0	38.0	41.0	34.0	41.0
19	38.4653848420134	40.0	37.0	41.0	34.0	41.0
20	38.4421873034225	40.0	37.0	41.0	34.0	41.0
21	38.370338873733346	40.0	37.0	41.0	34.0	41.0
22	38.29644563990107	40.0	37.0	41.0	34.0	41.0
23	38.08247456336902	40.0	37.0	41.0	34.0	41.0
24	37.8641662406658	40.0	36.0	41.0	33.0	41.0
25	37.836366300226295	40.0	36.0	41.0	33.0	41.0
26	38.04151398317973	40.0	37.0	41.0	34.0	41.0
27	38.05606330611705	40.0	37.0	41.0	34.0	41.0
28	38.05801415119129	40.0	37.0	41.0	34.0	41.0
29	38.1029942684968	40.0	37.0	41.0	34.0	41.0
30	38.0032981225704	40.0	37.0	41.0	34.0	41.0
31	37.901449676955984	40.0	37.0	41.0	34.0	41.0
32	37.83898282141566	40.0	37.0	41.0	34.0	41.0
33	37.7841346130877	40.0	36.0	41.0	33.0	41.0
34	37.66166613317026	40.0	36.0	41.0	33.0	41.0
35	37.583965168258395	40.0	36.0	41.0	33.0	41.0
36	37.540743200309585	40.0	36.0	41.0	33.0	41.0
37	37.431199666206425	40.0	36.0	41.0	33.0	41.0
38	37.423390712066414	40.0	36.0	41.0	33.0	41.0
39	37.38822533614255	40.0	36.0	41.0	33.0	41.0
40	37.31878799171249	39.0	36.0	41.0	33.0	41.0
41	37.28862314928504	39.0	35.0	41.0	33.0	41.0
42	37.277428483612105	39.0	36.0	41.0	33.0	41.0
43	37.18374810688402	39.0	35.0	41.0	32.0	41.0
44	37.05941875964474	39.0	35.0	41.0	32.0	41.0
45	37.079976685410706	39.0	35.0	41.0	32.0	41.0
46	36.963703930196374	39.0	35.0	41.0	32.0	41.0
47	36.90625273715508	39.0	35.0	41.0	32.0	41.0
48	36.85567568514475	39.0	35.0	41.0	31.0	41.0
49	36.81933263580669	39.0	35.0	41.0	31.0	41.0
50	36.73981061673777	39.0	35.0	41.0	31.0	41.0
51	35.70757708226833	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	12.0
11	18.0
12	12.0
13	24.0
14	21.0
15	33.0
16	63.0
17	120.0
18	223.0
19	433.0
20	767.0
21	1180.0
22	1848.0
23	2793.0
24	3860.0
25	5133.0
26	6243.0
27	7788.0
28	9534.0
29	11829.0
30	15414.0
31	20132.0
32	26545.0
33	37393.0
34	67345.0
35	98071.0
36	91566.0
37	146856.0
38	280288.0
39	419942.0
40	364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.00867449234234	22.620964338552042	25.570960596114556	10.799400572991068
2	30.516551925125768	24.68400291113861	26.620435619723757	18.179009544011862
3	31.9206029942685	24.57149090746943	26.66916693341487	16.838739164847205
4	28.949028001395057	26.165848904262077	26.646791934808334	18.23833115953454
5	25.152364981614276	29.769019943210502	25.219091845786096	19.859523229389122
6	25.73037250789495	33.76331551296078	24.69196554409467	15.8143464350496
7	80.2634198234525	6.288409750721813	7.974576905497879	5.473593520327805
8	80.19804660688322	4.281348487816375	8.724418050970407	6.796186854329999
9	73.79202876740034	7.823764637310031	11.252952146168461	7.1312544491211645
10	37.603534135011216	31.843126575605996	17.24507232459514	13.308266964787643
11	25.701707029253125	24.269308986786804	29.2798754007195	20.74910858324057
12	24.595936190644544	22.389331345860146	32.453701270676966	20.56103119281834
13	21.93291322481857	26.0788173260523	33.003361823634044	18.984907625495076
14	17.896017568753354	29.415080908313467	31.00959815776524	21.67930336516794
15	17.127145730515835	28.13078783882994	35.55028960096061	19.191776829693612
16	19.606231875056736	27.700407527554695	31.415612812194933	21.277747785193643
17	19.464098876790995	27.04468470362284	27.889201554942964	25.602014864643202
18	19.123616691589707	26.436658051097805	33.13848770489845	21.301237552414033
19	20.821409290481625	27.508269194324875	29.531733481119797	22.1385880340737
20	21.48947419549538	29.085587156591547	29.3221569817162	20.10278166619687
21	21.941035110433756	27.919141054857764	30.559470516758953	19.580353317949527
22	19.96399297377268	26.396048623021883	28.678059601900202	24.961898801305235
23	18.33977510339479	27.83226872930711	29.105573365311265	24.722382801986836
24	20.59949070999613	26.01089606693708	30.391538587715566	22.998074635351223
25	17.927310716270686	28.84248797244292	28.43918061321829	24.791020698068106
26	18.29375108490874	28.582587632757	27.86634879835906	25.257312483975202
27	20.340545886264934	29.53308712872233	28.197912834649557	21.928454150363173
28	18.67938139897091	27.253066808083027	29.46046791616303	24.60708387678303
29	18.52466744063459	25.910646518020236	28.40223399630215	27.162452045043022
30	22.32841720374626	27.88729052303351	26.62250590429234	23.161786368927896
31	22.552406068800334	28.50678336701527	25.127521566791362	23.813288997393034
32	21.22861833985473	28.54420774190877	24.681056736944864	25.546117181291635
33	24.7277177660674	26.965695384698684	25.3669579397802	22.939628909453717
34	21.69905069489898	24.541471781225066	28.885804695723905	24.87367282815205
35	19.929912904720727	27.13099964486657	25.809521079478227	27.129566370934477
36	25.24329824997253	26.002694554992335	26.43562290881352	22.31838428622162
37	21.953138312526974	27.325128636335407	28.91741634855948	21.80431670257814
38	22.25221480635673	28.222915502131595	24.907354765556196	24.617514925955476
39	24.270662634389335	24.029952240127532	28.585931938598545	23.113453186884588
40	22.93891227248767	24.645384141301697	29.512862041013932	22.9028415451967
41	20.21983237065101	26.914256775802514	26.60164380594745	26.264267047599027
42	22.652257486069374	25.576614065513358	27.111730073112895	24.659398375304374
43	24.449264491593848	24.703272482892285	27.40316243930483	23.44430058620904
44	22.11868145168354	24.120885508485777	27.629858599563967	26.13057444026672
45	22.41489139764911	23.353765449498592	26.639068180840948	27.592274972011342
46	23.712641316828385	24.621098110785706	28.47182740833815	23.194433164047755
47	20.05389109984664	24.74037835246754	30.900430459937606	24.305300087748215
48	20.939734016208735	23.60689755117186	29.715988807723114	25.73737962489629
49	23.210358429959886	21.338741553637092	31.208106597359908	24.242793419043114
50	20.969355010805295	22.356923429728965	30.17814002449306	26.49558153497268
51	20.025942258170858	22.072100048890565	27.562335472096542	30.33962222084203
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	863.0
1	1046.0
2	1229.0
3	1823.0
4	2417.0
5	1883.5
6	1350.0
7	1478.0
8	1606.0
9	1783.5
10	1961.0
11	2050.5
12	2140.0
13	2340.5
14	2541.0
15	2497.5
16	2454.0
17	2500.0
18	2546.0
19	3046.5
20	3547.0
21	3776.0
22	4005.0
23	4734.5
24	5464.0
25	6741.5
26	10013.5
27	12008.0
28	15398.5
29	18789.0
30	20236.0
31	21683.0
32	24016.0
33	26349.0
34	28739.5
35	31130.0
36	34789.0
37	38448.0
38	42081.5
39	45715.0
40	50970.0
41	56225.0
42	63168.0
43	70111.0
44	75698.5
45	81286.0
46	88842.5
47	96399.0
48	109684.5
49	122970.0
50	141784.5
51	160599.0
52	155438.5
53	150278.0
54	121453.0
55	92628.0
56	75512.5
57	58397.0
58	49238.5
59	40080.0
60	34817.0
61	29554.0
62	25530.5
63	21507.0
64	18408.5
65	15310.0
66	12183.5
67	9057.0
68	7968.5
69	6880.0
70	5760.5
71	4641.0
72	3570.0
73	2499.0
74	2125.0
75	1242.5
76	734.0
77	545.5
78	357.0
79	290.5
80	224.0
81	154.5
82	85.0
83	52.0
84	19.0
85	12.5
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1255866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.131477841254423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.873685619016705	12.657402088372315
2	12.966988736575807	5.220892928363431
3	6.453201332811993	3.897374389099743
4	3.829014055814766	3.0833484647394673
5	2.524766872496794	2.5413644174001218
6	1.773811229052441	2.1425664871342494
7	1.3167461627906407	1.8555632339143602
8	0.9639264759441709	1.5524211592854833
9	0.7348904226945522	1.3314987234202926
>10	4.477489604308011	17.712220783054015
>50	1.128852209441898	16.91755861562083
>100	0.9398443443320288	26.85666965015603
>500	0.011588216830624169	1.4941689718050872
>1k	0.004395530521960891	1.6191791248246366
>5k	7.99187367629253E-4	1.1177709628099326
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	7416	0.5905088600216902	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	6479	0.5158989892233725	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	4792	0.3815693712545765	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	3124	0.24875265354743262	No Hit
ACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	2367	0.18847552207002977	No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG	1506	0.11991725231832058	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCC	1348	0.10733629224773981	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG	1338	0.10654002895213342	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG	1323	0.10534563400872386	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.962632956063784E-5	0.0	0.0	0.40243146959946363	0.0
2	7.962632956063784E-5	0.0	0.0	2.0320639303874777	0.0
3	7.962632956063784E-5	0.0	0.0	2.8366083642681623	0.0
4	7.962632956063784E-5	0.0	0.0	3.823178587524465	0.0
5	7.962632956063784E-5	0.0	0.0	6.331646847673239	0.0
6	7.962632956063784E-5	0.0	0.0	7.552477732496938	0.0
7	7.962632956063784E-5	0.0	0.0	8.896888680798748	0.0
8	7.962632956063784E-5	0.0	0.0	10.925449052685558	0.0
9	7.962632956063784E-5	0.0	0.0	11.641210129106131	0.0
10	7.962632956063784E-5	0.0	0.0	13.266224262779629	0.0
11	7.962632956063784E-5	0.0	0.0	15.763067078812549	0.0
12	7.962632956063784E-5	0.0	0.0	17.663030928458927	0.0
13	7.962632956063784E-5	0.0	0.0	18.38126042109588	0.0
14	7.962632956063784E-5	0.0	0.0	18.672692787287815	0.0
15	1.5925265912127568E-4	0.0	0.0	19.207065084969255	0.0
16	1.5925265912127568E-4	0.0	0.0	20.334175779900086	0.0
17	1.5925265912127568E-4	0.0	0.0	21.76896261225322	0.0
18	1.5925265912127568E-4	0.0	0.0	23.339353083848117	0.0
19	2.388789886819135E-4	0.0	0.0	24.3182791794666	0.0
20	2.388789886819135E-4	0.0	0.0	25.153001992250765	0.0
21	2.388789886819135E-4	0.0	0.0	26.23193875779741	0.0
22	3.1850531824255136E-4	0.0	0.0	27.254261203026438	0.0
23	3.1850531824255136E-4	0.0	0.0	28.300312294464536	0.0
24	3.1850531824255136E-4	0.0	0.0	29.1196672256435	0.0
25	3.1850531824255136E-4	0.0	0.0	29.878904278004182	0.0
26	3.1850531824255136E-4	0.0	0.0	30.5324771910379	0.0
27	3.1850531824255136E-4	0.0	0.0	31.197914427176148	0.0
28	3.981316478031892E-4	0.0	0.0	31.840498906730495	0.0
29	3.981316478031892E-4	0.0	0.0	32.54471416536477	0.0
30	3.981316478031892E-4	0.0	0.0	33.35204552078008	0.0
31	3.981316478031892E-4	0.0	0.0	34.046625993537525	0.0
32	3.981316478031892E-4	0.0	0.0	34.711903977016654	0.0
33	3.981316478031892E-4	0.0	0.0	35.343181517773395	0.0
34	3.981316478031892E-4	0.0	0.0	35.96848708381308	0.0
35	3.981316478031892E-4	0.0	0.0	36.67413561637945	0.0
36	3.981316478031892E-4	0.0	0.0	37.31679972226336	0.0
37	3.981316478031892E-4	0.0	0.0	37.98175920042425	0.0
38	3.981316478031892E-4	0.0	0.0	38.584132383550475	0.0
39	3.981316478031892E-4	0.0	0.0	39.19407006798496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	30	2.1656633E-6	45.000004	26
TCGTCAC	30	2.1656633E-6	45.000004	14
TATCGAT	30	2.1656633E-6	45.000004	18
CCGCATT	30	2.1656633E-6	45.000004	27
TACGTAG	30	2.1656633E-6	45.000004	10
GTGTTCG	35	1.2120472E-7	45.0	1
TTTAGCG	55	1.8189894E-12	45.0	1
ACTATCG	25	3.8910126E-5	45.0	33
TCGTTAG	20	7.033536E-4	45.0	1
TACCCGT	20	7.033536E-4	45.0	31
TCGCAAT	20	7.033536E-4	45.0	30
CGTATTA	25	3.8910126E-5	45.0	38
CGAGTAT	20	7.033536E-4	45.0	24
CCGATTA	20	7.033536E-4	45.0	10
GCGGTAT	20	7.033536E-4	45.0	14
ATTACGA	20	7.033536E-4	45.0	14
TAGCGAG	25	3.8910126E-5	45.0	1
GAATCGT	25	3.8910126E-5	45.0	43
ATTCGGT	20	7.033536E-4	45.0	29
CGTCTAC	35	1.2120472E-7	45.0	22
>>END_MODULE