##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934978.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1429785
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14837335683337	33.0	31.0	34.0	30.0	34.0
2	32.317409260832925	34.0	31.0	34.0	30.0	34.0
3	32.333658557055784	34.0	31.0	34.0	30.0	34.0
4	35.87857614956095	37.0	35.0	37.0	35.0	37.0
5	35.80483289445616	37.0	35.0	37.0	35.0	37.0
6	35.74033928178013	37.0	35.0	37.0	35.0	37.0
7	36.04051378354088	37.0	35.0	37.0	35.0	37.0
8	35.776758044041586	37.0	35.0	37.0	35.0	37.0
9	37.486368929594306	39.0	37.0	39.0	35.0	39.0
10	37.31154614155275	39.0	37.0	39.0	34.0	39.0
11	37.26831236864284	39.0	37.0	39.0	34.0	39.0
12	37.1353930835755	39.0	37.0	39.0	34.0	39.0
13	36.998628465118884	39.0	37.0	39.0	33.0	39.0
14	38.090974517147686	40.0	37.0	41.0	33.0	41.0
15	38.14719975380914	40.0	37.0	41.0	33.0	41.0
16	38.203068992890536	40.0	37.0	41.0	34.0	41.0
17	38.21734176816794	40.0	37.0	41.0	34.0	41.0
18	38.229119762761535	40.0	37.0	41.0	34.0	41.0
19	38.218829404420944	40.0	37.0	41.0	34.0	41.0
20	38.1853054829922	40.0	37.0	41.0	34.0	41.0
21	38.10463810992562	40.0	37.0	41.0	34.0	41.0
22	38.03801830345122	40.0	36.0	41.0	34.0	41.0
23	37.8137104529702	40.0	36.0	41.0	33.0	41.0
24	37.58001517710705	39.0	35.0	41.0	33.0	41.0
25	37.55241102683271	39.0	35.0	41.0	33.0	41.0
26	37.74485604478995	39.0	36.0	41.0	33.0	41.0
27	37.770808897841285	40.0	36.0	41.0	33.0	41.0
28	37.69847214791035	40.0	36.0	41.0	33.0	41.0
29	37.724585164902415	40.0	36.0	41.0	33.0	41.0
30	37.65523068153603	40.0	36.0	41.0	33.0	41.0
31	37.528221375941136	39.0	36.0	41.0	33.0	41.0
32	37.4643411421997	39.0	35.0	41.0	33.0	41.0
33	37.41319638966698	39.0	35.0	41.0	33.0	41.0
34	37.296747413072595	39.0	35.0	41.0	33.0	41.0
35	37.23283780428526	39.0	35.0	41.0	32.0	41.0
36	37.19795843431005	39.0	35.0	41.0	32.0	41.0
37	37.10642439247859	39.0	35.0	41.0	32.0	41.0
38	37.001406505173854	39.0	35.0	41.0	32.0	41.0
39	37.014724591459554	39.0	35.0	41.0	32.0	41.0
40	36.956619351860596	39.0	35.0	41.0	32.0	41.0
41	36.88261102193687	39.0	35.0	41.0	32.0	41.0
42	36.86337596212018	39.0	35.0	41.0	32.0	41.0
43	36.7867189822246	39.0	35.0	41.0	31.0	41.0
44	36.63094451263652	39.0	35.0	41.0	31.0	41.0
45	36.61923645862839	39.0	35.0	41.0	31.0	41.0
46	36.48972817591456	39.0	35.0	41.0	31.0	41.0
47	36.42554370062632	39.0	35.0	40.0	31.0	41.0
48	36.40265844165381	39.0	35.0	40.0	31.0	41.0
49	36.3740527421955	39.0	35.0	40.0	31.0	41.0
50	36.276598929209634	39.0	35.0	40.0	31.0	41.0
51	35.22924495640953	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	15.0
10	33.0
11	15.0
12	27.0
13	24.0
14	30.0
15	45.0
16	108.0
17	202.0
18	341.0
19	634.0
20	1132.0
21	1841.0
22	2961.0
23	4278.0
24	5924.0
25	7295.0
26	8560.0
27	10394.0
28	12466.0
29	15528.0
30	19453.0
31	25413.0
32	33828.0
33	47784.0
34	89275.0
35	124438.0
36	107660.0
37	169924.0
38	315580.0
39	424308.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43986683312526	22.342939672748	24.760715771951727	11.456477722175013
2	31.61006724787293	24.78680361033302	24.20203037519627	19.401098766597773
3	30.32875572201415	24.525855285934597	26.84095860566449	18.304430386386763
4	28.581150312809267	25.469004081033162	26.026710309591998	19.923135296565565
5	24.683081722077098	28.72340946366062	25.461240676045698	21.132268138216585
6	26.145399483139077	32.23967239829765	25.58706378931098	16.0278643292523
7	77.04871711481097	6.538395632909843	10.180551621397623	6.2323356308815665
8	75.20487345999574	5.1245466975804055	11.162797203775392	8.507782638648468
9	69.08451270645587	7.503995355945124	13.971191472843818	9.44030046475519
10	36.873795710543895	26.05391719734086	20.925733589315875	16.14655350279937
11	28.065898019632325	24.45332689879947	25.46145049780212	22.01932458376609
12	25.61986592389765	22.183405197284905	31.546840958605664	20.64988792021178
13	22.874977706438383	26.276538080900274	32.05929562836371	18.78918858429764
14	18.664274698643503	29.37609500729131	30.133271785618117	21.826358508447075
15	16.51674902170606	26.457614256688945	36.3919050766374	20.6337316449676
16	19.172183230345823	27.385655885325416	30.96514510922971	22.477015775099055
17	18.628605000052456	26.89558220291862	27.791940746335985	26.683872050692937
18	20.711715397769595	25.107341313554137	32.18232111821008	21.998622170466188
19	20.074346842357418	27.594078830033887	28.204730081795514	24.12684424581318
20	22.54499802417846	28.43455484565861	28.499389768391755	20.521057361771174
21	21.013299202327623	27.85894382721878	31.226163374213606	19.901593596239994
22	20.066653377955426	25.47075259566998	27.785506212472505	26.67708781390209
23	18.632032088740615	26.771647485461102	29.144661609962334	25.45165881583595
24	21.17535153886773	24.655315309644457	30.032207639610153	24.13712551187766
25	18.439905300447272	27.596246988183537	27.633175617313093	26.3306720940561
26	18.18462216347213	27.590511860174782	27.50812185048801	26.716744125865077
27	22.153680448459035	26.504054805442777	27.66926495941697	23.672999786681213
28	17.64139363610613	25.910469056536474	30.41065614760261	26.037481159754787
29	18.85863958567197	23.472200365789263	30.43807285710789	27.23108719143088
30	22.50527177163	24.706581758795902	27.766342492053003	25.021803977521095
31	20.98378427525817	26.207856425966142	28.236972691698405	24.57138660707729
32	20.713254090649993	26.445724357158596	27.632406270872895	25.20861528131852
33	20.589878897876254	24.29840850197757	27.086380120087984	28.02533248005819
34	17.747703326024542	22.834132404522357	32.96041013159321	26.45775413785989
35	18.063834772360877	24.893393062593326	28.671653430410863	28.371118734634926
36	21.44811982221103	24.305542441695778	27.926296611028935	26.320041125064257
37	19.83501015887004	24.359396692509712	33.209048912948454	22.596544235671796
38	18.091111600695207	27.143381697248188	28.949667257664615	25.81583944439199
39	20.162262158296524	21.67983298188189	30.95661235780205	27.201292502019538
40	21.39188759149103	22.4896750210696	31.12195190185937	24.99648548558
41	18.5753102739223	25.746248561846713	26.91621467563305	28.762226488597936
42	20.600230104526204	22.862598222809723	29.01023580468392	27.52693586798015
43	22.671660424469415	21.300195483936395	30.1576111093626	25.87053298223159
44	20.449508142832666	21.658221340970847	29.579901873358583	28.31236864283791
45	20.788719982374975	21.06533499791927	28.73669817490042	29.409246844805338
46	22.645922289015484	22.31741135905049	29.25782547725707	25.778840874676963
47	17.345475018971385	23.718531107823903	33.039792696104655	25.896201177100053
48	18.91529145990481	21.514843140751932	31.687771238333035	27.882094161010222
49	21.57995782582696	18.695608080935244	33.734722353360816	25.989711739876974
50	19.50230279377669	20.42530870025913	32.605461660319556	27.466926845644622
51	18.615386229398126	19.893830191252533	28.863640337533266	32.62714324181608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1480.0
1	1746.0
2	2012.0
3	2669.0
4	3326.0
5	2625.0
6	1924.0
7	2075.0
8	2226.0
9	2366.5
10	2507.0
11	2831.5
12	3156.0
13	3345.0
14	3534.0
15	3527.0
16	3520.0
17	3553.0
18	3586.0
19	3699.5
20	3813.0
21	4017.5
22	4222.0
23	5585.5
24	6949.0
25	6970.0
26	8426.0
27	9861.0
28	11753.0
29	13645.0
30	17207.0
31	20769.0
32	21942.5
33	23116.0
34	25610.0
35	28104.0
36	28640.0
37	29176.0
38	34913.5
39	40651.0
40	47199.5
41	53748.0
42	65160.5
43	76573.0
44	89796.5
45	103020.0
46	127612.0
47	152204.0
48	174774.5
49	197345.0
50	194361.5
51	191378.0
52	157615.0
53	123852.0
54	103604.0
55	83356.0
56	71745.0
57	60134.0
58	52838.0
59	45542.0
60	41432.0
61	37322.0
62	32523.5
63	27725.0
64	24626.0
65	21527.0
66	17837.0
67	14147.0
68	11412.0
69	8677.0
70	7618.0
71	6559.0
72	5777.0
73	4995.0
74	3766.0
75	2303.0
76	2069.0
77	1644.5
78	1220.0
79	1047.5
80	875.0
81	504.5
82	134.0
83	170.5
84	207.0
85	120.0
86	33.0
87	26.5
88	20.0
89	16.0
90	12.0
91	7.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1429785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.26199437786876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.275856837354105	12.820950554932974
2	12.264267089713679	4.969970216409189
3	6.33422016382543	3.8503180004284148
4	3.8076202305594524	3.0859991881861992
5	2.531118220615391	2.564275157791512
6	1.8168158622130477	2.208738767147014
7	1.3532593030243896	1.919381267277515
8	1.0377707215540977	1.6821843620516748
9	0.8189680890446428	1.4934534134288828
>10	4.828106493082843	18.87236427378807
>50	1.0862694902597112	16.399977940541945
>100	0.8230678584324883	23.92721628319442
>500	0.014293003894668477	1.9104731870641816
>1k	0.007320806872878976	2.440591176819669
>5k	6.9721970217895E-4	1.1317189116405633
>10k+	3.48609851089475E-4	0.7223872992977182
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	10227	0.7152823676286995	TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	9397	0.6572316816864073	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	6625	0.4633563787562466	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	4145	0.2899037267840969	TruSeq Adapter, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3217	0.2249988634654861	TruSeq Adapter, Index 22 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	2300	0.16086334658707427	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	2191	0.15323982277055642	TruSeq Adapter, Index 23 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1944	0.1359644981588141	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1809	0.12652251912000756	No Hit
GGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1716	0.12001804467105194	TruSeq Adapter, Index 23 (95% over 22bp)
GGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1613	0.11281416436736992	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC	1518	0.10616980874746902	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	1512	0.10575016523463318	No Hit
AGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1434	0.10029479956776717	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0982175641792296E-4	0.0	0.0	0.48349926737236715	0.0
2	2.0982175641792296E-4	0.0	0.0	2.4532359760383553	0.0
3	2.0982175641792296E-4	0.0	0.0	3.4970292736320494	0.0
4	2.0982175641792296E-4	0.0	0.0	4.759456841413219	0.0
5	2.0982175641792296E-4	0.0	0.0	8.006028878467742	0.0
6	2.0982175641792296E-4	0.0	0.0	9.651730854638984	0.0
7	2.0982175641792296E-4	0.0	0.0	11.318904590550327	0.0
8	2.0982175641792296E-4	0.0	0.0	13.643310008148077	0.0
9	2.0982175641792296E-4	0.0	0.0	14.4802190539137	0.0
10	2.0982175641792296E-4	0.0	0.0	16.34595411198187	0.0
11	2.0982175641792296E-4	0.0	0.0	19.293040562042545	0.0
12	2.0982175641792296E-4	0.0	0.0	21.465465087408248	0.0
13	2.0982175641792296E-4	0.0	0.0	22.320768507153172	0.0
14	2.0982175641792296E-4	0.0	0.0	22.686068185076778	0.0
15	2.0982175641792296E-4	0.0	0.0	23.39904251338488	0.0
16	2.0982175641792296E-4	0.0	0.0	24.74462943729302	0.0
17	2.0982175641792296E-4	0.0	0.0	26.315494987008535	0.0
18	4.895840983084869E-4	0.0	0.0	28.01071489769441	0.0
19	5.595246837811279E-4	0.0	0.0	29.041289424633774	0.0
20	5.595246837811279E-4	0.0	0.0	29.96618372692398	0.0
21	5.595246837811279E-4	0.0	0.0	31.136289721881262	0.0
22	5.595246837811279E-4	0.0	0.0	32.21386432225824	0.0
23	5.595246837811279E-4	0.0	0.0	33.231989424983475	0.0
24	5.595246837811279E-4	0.0	0.0	34.136880719828504	0.0
25	5.595246837811279E-4	0.0	0.0	34.896155715719495	0.0
26	5.595246837811279E-4	0.0	0.0	35.55905258482919	0.0
27	5.595246837811279E-4	0.0	0.0	36.22992268068276	0.0
28	5.595246837811279E-4	0.0	0.0	36.89177044101036	0.0
29	5.595246837811279E-4	0.0	0.0	37.61824330231468	0.0
30	5.595246837811279E-4	0.0	0.0	38.40367607717244	0.0
31	6.29465269253769E-4	0.0	0.0	39.140570085712184	0.0
32	6.29465269253769E-4	0.0	0.0	39.80584493472795	0.0
33	6.29465269253769E-4	0.0	0.0	40.462027507632264	0.0
34	6.994058547264099E-4	0.0	0.0	41.09715796430932	0.0
35	6.994058547264099E-4	0.0	0.0	41.78628255297125	0.0
36	7.693464401990509E-4	0.0	0.0	42.401270121032184	0.0
37	7.693464401990509E-4	0.0	0.0	43.02744818276874	0.0
38	7.693464401990509E-4	0.0	0.0	43.64075717677833	0.0
39	7.693464401990509E-4	0.0	0.0	44.29204390869956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.033864E-4	45.000004	2
CGAGTTT	30	2.1658743E-6	45.000004	27
GTCGACG	20	7.033864E-4	45.000004	1
TACCACG	20	7.033864E-4	45.000004	25
GCGAAGT	30	2.1658743E-6	45.000004	29
CGGCACA	20	7.033864E-4	45.000004	24
ACTTCGC	20	7.033864E-4	45.000004	40
TCGATAT	20	7.033864E-4	45.000004	17
TAGCACG	30	2.1658743E-6	45.000004	1
AATGCGC	20	7.033864E-4	45.000004	38
CCCGTAA	20	7.033864E-4	45.000004	16
TGACCGA	40	6.8175723E-9	45.000004	25
GTATGCG	55	1.8189894E-12	45.000004	1
CTACGAT	20	7.033864E-4	45.000004	26
TCGTAGT	30	2.1658743E-6	45.000004	17
ACCACGT	20	7.033864E-4	45.000004	13
CCGACAA	20	7.033864E-4	45.000004	36
TTACGTA	20	7.033864E-4	45.000004	13
TTTGCGA	20	7.033864E-4	45.000004	1
CAATGCG	30	2.1658743E-6	45.000004	1
>>END_MODULE