##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934977.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1093905
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08598187228324	33.0	31.0	34.0	30.0	34.0
2	32.27319831246772	34.0	31.0	34.0	30.0	34.0
3	32.269018790479976	34.0	31.0	34.0	30.0	34.0
4	35.85001165549111	37.0	35.0	37.0	35.0	37.0
5	35.76134856317505	37.0	35.0	37.0	35.0	37.0
6	35.691956796979625	37.0	35.0	37.0	33.0	37.0
7	36.01538707657429	37.0	35.0	37.0	35.0	37.0
8	35.74206261055576	37.0	35.0	37.0	35.0	37.0
9	37.499377916729514	39.0	37.0	39.0	35.0	39.0
10	37.29386007011578	39.0	37.0	39.0	34.0	39.0
11	37.24015888034153	39.0	37.0	39.0	34.0	39.0
12	37.14219699151206	39.0	37.0	39.0	34.0	39.0
13	37.074050306013774	39.0	37.0	39.0	33.0	39.0
14	38.15269698922667	40.0	37.0	41.0	33.0	41.0
15	38.21470877269964	40.0	37.0	41.0	33.0	41.0
16	38.27453755124988	40.0	37.0	41.0	34.0	41.0
17	38.27997038134024	40.0	37.0	41.0	34.0	41.0
18	38.27826639424813	40.0	37.0	41.0	34.0	41.0
19	38.22987096685727	40.0	37.0	41.0	34.0	41.0
20	38.19284672800654	40.0	37.0	41.0	34.0	41.0
21	38.14664801788089	40.0	37.0	41.0	34.0	41.0
22	38.06690891805047	40.0	37.0	41.0	34.0	41.0
23	37.800033823778115	40.0	36.0	41.0	33.0	41.0
24	37.565299546121466	39.0	36.0	41.0	33.0	41.0
25	37.52730538757936	39.0	36.0	41.0	33.0	41.0
26	37.70422934349875	39.0	36.0	41.0	33.0	41.0
27	37.78332487738881	39.0	36.0	41.0	33.0	41.0
28	37.77806573696984	40.0	36.0	41.0	33.0	41.0
29	37.81061792385993	40.0	36.0	41.0	33.0	41.0
30	37.75774770203994	40.0	36.0	41.0	33.0	41.0
31	37.658786640521804	40.0	36.0	41.0	33.0	41.0
32	37.58788834496597	40.0	36.0	41.0	33.0	41.0
33	37.55274543950343	40.0	36.0	41.0	33.0	41.0
34	37.4026053450711	40.0	36.0	41.0	33.0	41.0
35	37.31698456447315	40.0	36.0	41.0	33.0	41.0
36	37.27945205479452	40.0	36.0	41.0	32.0	41.0
37	37.204707904251286	39.0	35.0	41.0	32.0	41.0
38	37.13070696267043	39.0	35.0	41.0	32.0	41.0
39	37.09033325563006	39.0	35.0	41.0	32.0	41.0
40	37.005358783440975	39.0	35.0	41.0	31.0	41.0
41	36.94925427710816	39.0	35.0	41.0	31.0	41.0
42	36.937129823887815	39.0	35.0	41.0	31.0	41.0
43	36.84940556995352	39.0	35.0	41.0	31.0	41.0
44	36.689253637198846	39.0	35.0	41.0	31.0	41.0
45	36.71036973046105	39.0	35.0	41.0	31.0	41.0
46	36.59887650207285	39.0	35.0	41.0	31.0	41.0
47	36.51191191191191	39.0	35.0	41.0	31.0	41.0
48	36.480622174686104	39.0	35.0	40.0	31.0	41.0
49	36.44839085661003	39.0	35.0	40.0	31.0	41.0
50	36.34881273968032	39.0	35.0	40.0	31.0	41.0
51	35.270479612032126	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	19.0
11	10.0
12	16.0
13	15.0
14	20.0
15	36.0
16	64.0
17	152.0
18	254.0
19	440.0
20	908.0
21	1429.0
22	2242.0
23	3354.0
24	4697.0
25	5933.0
26	6779.0
27	7873.0
28	9289.0
29	11653.0
30	14630.0
31	19305.0
32	25258.0
33	35585.0
34	64701.0
35	89511.0
36	82529.0
37	129718.0
38	246648.0
39	330608.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.44986539050466	23.88552936498142	25.977758580498307	11.686846664015613
2	30.63904086735137	25.675172889784765	24.817511575502444	18.868274667361426
3	29.010471658873488	25.961120938289888	27.858269228132244	17.170138174704384
4	27.519574368889437	26.102540897061445	27.467741714317057	18.91014301973206
5	24.709001238681605	28.332533446688696	26.054547698383313	20.903917616246385
6	25.121559916080464	33.083585868974	25.802423427994203	15.992430786951333
7	76.36056147471673	6.392876895160001	10.996567343599308	6.249994286523966
8	74.82249372660331	5.528907903337127	11.012382245258957	8.6362161248006
9	68.89574506012862	7.259131277396118	14.058167756797893	9.786955905677367
10	36.2465662009041	26.53018315118772	20.831516447954805	16.39173419995338
11	26.206114790589673	25.016614788304288	26.570588853693877	22.20668156741216
12	23.40450039080176	22.245990282519962	32.84078599147092	21.508723335207353
13	21.82291880922018	25.353572750833024	33.96538090602018	18.858127533926623
14	17.82430832659143	30.35994899008598	30.42741371508495	21.388328968237644
15	15.426476705015519	26.945941375165123	36.67164881776754	20.955933102051823
16	17.34849004255397	28.188096772571658	30.86456319333032	23.598849991544053
17	17.507187552849654	27.10866117258811	28.01129897020308	27.372852304359153
18	18.66642898606369	26.440778678221598	32.35308367728459	22.53970865843012
19	18.63324511726338	28.555953213487463	29.711629437656832	23.099172231592323
20	20.66468294778797	29.08168442415018	29.366718316490005	20.886914311571847
21	19.741019558371157	29.381984724450476	30.7152814915372	20.161714225641166
22	17.957866542341428	26.793003048710812	28.24623710468459	27.002893304263164
23	17.495486353933842	27.82673084042947	29.018150570661987	25.6596322349747
24	20.684154474108812	25.159497396940317	29.311686115339082	24.844662013611785
25	16.847166801504702	29.589498173973062	26.810280600234936	26.753054424287303
26	16.92359025692359	30.494147115151684	28.13635553361581	24.44590709430892
27	19.50617283950617	28.437935652547523	27.357037402699504	24.6988541052468
28	15.610222094240358	27.243956285052175	31.061289600102388	26.084532020605078
29	17.3442849241936	26.345249358947985	30.712356191808247	25.598109525050166
30	19.35561131908164	26.10830008090282	30.089084518308262	24.447004081707277
31	19.915989048409138	27.783217006961298	27.92756226546181	24.373231679167752
32	19.733797724665305	27.496994711606582	28.28773979458911	24.481467769139
33	21.11362504056568	23.26938810957076	29.567375594772855	26.049611255090703
34	20.538529396976884	24.114342653155436	30.829642427815944	24.517485522051732
35	18.260452233054973	24.163889917314574	28.374858877141985	29.200798972488467
36	22.86935337163648	23.628468651299702	29.114228383634778	24.387949593429045
37	20.73717553169608	22.955284051174463	33.164854352068964	23.142686065060495
38	19.49145492524488	23.78478935556561	31.741056124617767	24.98269959457174
39	24.15365136826324	21.75499700613856	32.27391775336981	21.817433872228392
40	23.962866976565607	21.93097206795837	30.725428624972007	23.38073233050402
41	21.368126116984566	24.016345112235523	25.59244175682532	29.023087013954594
42	22.88882489795732	22.105941557996353	28.465634584356046	26.539598959690284
43	23.920632961728852	20.81670711807698	30.572307467284638	24.69035245290953
44	20.243714033668372	21.88672690955796	30.582363185102913	27.287195871670754
45	21.1566817959512	20.75765262979875	29.87754878165837	28.20811679259168
46	24.40988934139619	21.248280243714035	30.22986456776411	24.111965847125663
47	18.963895402251566	22.274603370493782	33.61297370429791	25.148527522956748
48	19.683976213656578	20.388699201484588	32.38571905238572	27.541605532473113
49	22.594100950265332	17.98053761524081	34.95404079878966	24.471320635704195
50	20.898706926104186	18.0330101791289	33.18396021592368	27.884322678843226
51	19.27068621132548	18.36174073616996	29.270000594201505	33.097572458303055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1594.0
1	1638.5
2	1683.0
3	2199.0
4	2715.0
5	2167.0
6	1619.0
7	1814.5
8	2010.0
9	2175.5
10	2341.0
11	2582.0
12	2823.0
13	2922.5
14	3022.0
15	3254.0
16	3486.0
17	3244.5
18	3003.0
19	3244.0
20	3485.0
21	3446.5
22	3408.0
23	3872.0
24	4336.0
25	5134.0
26	6799.0
27	7666.0
28	8152.5
29	8639.0
30	10383.0
31	12127.0
32	14787.5
33	17448.0
34	18584.0
35	19720.0
36	23369.0
37	27018.0
38	30372.5
39	33727.0
40	38892.5
41	44058.0
42	52589.5
43	61121.0
44	72681.5
45	84242.0
46	108659.5
47	133077.0
48	150164.0
49	167251.0
50	159770.5
51	152290.0
52	121015.0
53	89740.0
54	73164.5
55	56589.0
56	46182.5
57	35776.0
58	32211.5
59	28647.0
60	26207.0
61	23767.0
62	20903.5
63	18040.0
64	14758.5
65	11477.0
66	9183.5
67	6890.0
68	5893.5
69	4897.0
70	3922.5
71	2948.0
72	2441.0
73	1934.0
74	1643.5
75	1198.5
76	1044.0
77	866.5
78	689.0
79	446.5
80	204.0
81	118.0
82	32.0
83	22.0
84	12.0
85	16.5
86	21.0
87	11.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1093905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.857536810738534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.35350948140519	13.628941287685484
2	13.045395758868938	5.702804360202603
3	6.536231575261571	4.285977667793739
4	4.020110312691407	3.514788365715281
5	2.5924896501722157	2.8332718980048934
6	1.7778897883828693	2.331617489700882
7	1.329495899028383	2.034165388691716
8	0.9117866959066597	1.5943529015457183
9	0.7670025266839422	1.5088307364831357
>10	4.918897288859236	21.569192801750532
>50	1.288426653871061	20.18150813348542
>100	0.4359528809137404	13.583698366059554
>500	0.01436278871227435	2.08656096031141
>1k	0.007181394356137175	3.030397854563257
>5k	0.0012673048863771485	2.113891788006435
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	9087	0.8306937074060362	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	7511	0.6866226957551158	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	6296	0.5755527216714431	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3808	0.3481106677453709	No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3004	0.2746125120554344	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	2769	0.25312984217093804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	2724	0.24901613942709833	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	2466	0.22543091036241722	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	2146	0.19617791307289023	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	2009	0.1836539736083115	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1946	0.1778947897669359	No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1737	0.15878892591221358	No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1735	0.15860609467915404	No Hit
GGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1470	0.13438095629876454	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1353	0.12368532916478123	No Hit
AAAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1260	0.11518367682751243	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1198	0.10951590860266659	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1107	0.10119708749845735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8283123305954356E-4	0.0	0.0	0.5999606912848922	0.0
2	1.8283123305954356E-4	0.0	0.0	2.8311416439270323	0.0
3	1.8283123305954356E-4	0.0	0.0	4.08152444682125	0.0
4	1.8283123305954356E-4	0.0	0.0	5.550847651304272	0.0
5	1.8283123305954356E-4	0.0	0.0	9.205735415781078	0.0
6	1.8283123305954356E-4	0.0	0.0	11.016038869920148	0.0
7	1.8283123305954356E-4	0.0	0.0	12.844351200515584	0.0
8	1.8283123305954356E-4	0.0	0.0	15.56606835145648	0.0
9	1.8283123305954356E-4	0.0	0.0	16.497684899511384	0.0
10	1.8283123305954356E-4	0.0	0.0	18.85895027447539	0.0
11	1.8283123305954356E-4	0.0	0.0	22.358340075235052	0.0
12	1.8283123305954356E-4	0.0	0.0	25.164890918315574	0.0
13	1.8283123305954356E-4	0.0	0.0	26.109305652684647	0.0
14	1.8283123305954356E-4	0.0	0.0	26.487674889501374	0.0
15	1.8283123305954356E-4	0.0	0.0	27.18965540883349	0.0
16	1.8283123305954356E-4	0.0	0.0	28.64060407439403	0.0
17	1.8283123305954356E-4	0.0	0.0	30.37457548873074	0.0
18	2.7424684958931537E-4	0.0	0.0	32.350706871254815	0.0
19	2.7424684958931537E-4	0.0	0.0	33.50336638007871	0.0
20	2.7424684958931537E-4	0.0	0.0	34.518902464107946	0.0
21	2.7424684958931537E-4	0.0	0.0	35.73491299518697	0.0
22	2.7424684958931537E-4	0.0	0.0	36.961253490933856	0.0
23	2.7424684958931537E-4	0.0	0.0	38.16117487350364	0.0
24	2.7424684958931537E-4	0.0	0.0	39.12432980926132	0.0
25	2.7424684958931537E-4	0.0	0.0	39.934455002948155	0.0
26	2.7424684958931537E-4	0.0	0.0	40.6792180308162	0.0
27	2.7424684958931537E-4	0.0	0.0	41.38503800604257	0.0
28	2.7424684958931537E-4	0.0	0.0	42.09241204674995	0.0
29	2.7424684958931537E-4	0.0	0.0	42.81441258610209	0.0
30	2.7424684958931537E-4	0.0	0.0	43.68944286752506	0.0
31	2.7424684958931537E-4	0.0	0.0	44.45376883733048	0.0
32	2.7424684958931537E-4	0.0	0.0	45.14697345747574	0.0
33	2.7424684958931537E-4	0.0	0.0	45.802332012377676	0.0
34	2.7424684958931537E-4	0.0	0.0	46.44160141877037	0.0
35	2.7424684958931537E-4	0.0	0.0	47.1313322454875	0.0
36	2.7424684958931537E-4	0.0	0.0	47.76676219598594	0.0
37	2.7424684958931537E-4	0.0	0.0	48.38061806098336	0.0
38	3.656624661190871E-4	0.0	0.0	49.03670794081753	0.0
39	3.656624661190871E-4	0.0	0.0	49.65577449595715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	65	0.0	45.000004	1
TAATACG	40	6.8139343E-9	45.000004	1
CCGGGTA	25	3.8906815E-5	45.000004	5
TCGCACA	25	3.8906815E-5	45.000004	19
TCCGACA	50	2.1827873E-11	45.000004	20
CGGTTTA	20	7.033136E-4	45.000004	13
CGTTGCC	25	3.8906815E-5	45.000004	40
ATCACTC	20	7.033136E-4	45.000004	38
CGTTCGC	20	7.033136E-4	45.000004	15
GCGCAAC	35	1.2118471E-7	45.000004	23
ATCGTTC	20	7.033136E-4	45.000004	44
CCCGTTG	20	7.033136E-4	45.000004	36
GCCGATT	25	3.8906815E-5	45.000004	9
TTCCCGA	20	7.033136E-4	45.000004	43
CCCGTAC	25	3.8906815E-5	45.000004	38
CCGTACT	25	3.8906815E-5	45.000004	39
GTTCGCC	20	7.033136E-4	45.000004	16
TAATTCG	20	7.033136E-4	45.000004	18
GTATGCG	25	3.8906815E-5	45.000004	1
CGCTACC	20	7.033136E-4	45.000004	34
>>END_MODULE