FastQCFastQC Report
Sat 14 Jan 2017
SRR2934976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158585
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC93290.8052063508503907TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC72400.6249002015389462TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC57710.49810760539796384No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC54360.4691930242494076No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT51870.4477012907986898TruSeq Adapter, Index 8 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG46960.40532200917498495No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC32890.2838807683510489TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC25010.21586676851504208TruSeq Adapter, Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG13800.11911081189554501TruSeq Adapter, Index 13 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG13790.119024499713012TruSeq Adapter, Index 13 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG13600.11738456824488491TruSeq Adapter, Index 8 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG13310.11488151495142782TruSeq Adapter, Index 8 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG13300.11479520276889482TruSeq Adapter, Index 8 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC12220.10547348705533043No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT12070.10417880431733537No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG207.0333085E-445.00000416
TGTTTCG207.0333085E-445.00000412
TCGCAAA207.0333085E-445.00000431
AGATCGC207.0333085E-445.00000422
GCGACTT351.211938E-745.00000442
ATAGGCC207.0333085E-445.00000425
CTATCGG850.045.0000042
GTTTACG406.8157533E-945.0000041
GCAACGA650.045.00000411
ATTACGC351.211938E-745.00000430
GAATCGT650.045.00000415
GAATCGC207.0333085E-445.0000047
CAACCCG207.0333085E-445.00000430
CTAAGTC207.0333085E-445.00000422
CGTTCGG207.0333085E-445.00000445
TACGCTT351.211938E-745.00000426
CCCGTCG207.0333085E-445.00000443
CCGTACG351.211938E-745.00000424
TACGAAC207.0333085E-445.00000442
CGAATAT1300.045.00000414
Produced by FastQC (version 0.11.5)