##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934976.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1158585
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14104446372083	33.0	31.0	34.0	30.0	34.0
2	32.31842204067893	34.0	31.0	34.0	30.0	34.0
3	32.325755123706934	34.0	31.0	34.0	30.0	34.0
4	35.887159768165475	37.0	35.0	37.0	35.0	37.0
5	35.80299158024659	37.0	35.0	37.0	35.0	37.0
6	35.735005200309	37.0	35.0	37.0	35.0	37.0
7	36.05804494275345	37.0	35.0	37.0	35.0	37.0
8	35.74900158382855	37.0	35.0	37.0	35.0	37.0
9	37.508337325271775	39.0	37.0	39.0	35.0	39.0
10	37.31289460850952	39.0	37.0	39.0	34.0	39.0
11	37.24103626406349	39.0	37.0	39.0	34.0	39.0
12	37.121692409275106	39.0	37.0	39.0	34.0	39.0
13	37.0300711643945	39.0	37.0	39.0	33.0	39.0
14	38.1231778419365	40.0	37.0	41.0	33.0	41.0
15	38.185719649399914	40.0	37.0	41.0	33.0	41.0
16	38.265712917049676	40.0	37.0	41.0	34.0	41.0
17	38.28157450683377	40.0	37.0	41.0	34.0	41.0
18	38.30301790546227	40.0	37.0	41.0	34.0	41.0
19	38.26663300491548	40.0	37.0	41.0	34.0	41.0
20	38.22849855642875	40.0	37.0	41.0	34.0	41.0
21	38.165607184626076	40.0	37.0	41.0	34.0	41.0
22	38.08618961923381	40.0	37.0	41.0	34.0	41.0
23	37.842525149212186	40.0	36.0	41.0	33.0	41.0
24	37.56787029005209	39.0	36.0	41.0	33.0	41.0
25	37.555323088077266	39.0	36.0	41.0	33.0	41.0
26	37.74274481371673	39.0	36.0	41.0	33.0	41.0
27	37.714958332793884	39.0	36.0	41.0	33.0	41.0
28	37.728237462076585	40.0	36.0	41.0	33.0	41.0
29	37.76878433606512	40.0	36.0	41.0	33.0	41.0
30	37.59265310702279	40.0	36.0	41.0	33.0	41.0
31	37.58217135557598	40.0	36.0	41.0	33.0	41.0
32	37.50484858685379	40.0	36.0	41.0	33.0	41.0
33	37.35362619056867	39.0	35.0	41.0	33.0	41.0
34	37.330061238493506	39.0	35.0	41.0	33.0	41.0
35	37.29409408891018	39.0	35.0	41.0	33.0	41.0
36	37.255227713115566	39.0	35.0	41.0	33.0	41.0
37	37.211757445504645	39.0	35.0	41.0	32.0	41.0
38	37.12930945938364	39.0	35.0	41.0	32.0	41.0
39	37.082190775817054	39.0	35.0	41.0	32.0	41.0
40	37.00753246416966	39.0	35.0	41.0	32.0	41.0
41	37.02466456928063	39.0	35.0	41.0	32.0	41.0
42	37.07108930289966	39.0	35.0	41.0	32.0	41.0
43	36.97230328374698	39.0	35.0	41.0	32.0	41.0
44	36.82354682651683	39.0	35.0	41.0	31.0	41.0
45	36.8205949498742	39.0	35.0	41.0	31.0	41.0
46	36.67540836451361	39.0	35.0	41.0	31.0	41.0
47	36.61439514580285	39.0	35.0	41.0	31.0	41.0
48	36.61802284683472	39.0	35.0	41.0	31.0	41.0
49	36.59298972453467	39.0	35.0	40.0	31.0	41.0
50	36.512694364246045	39.0	35.0	40.0	31.0	41.0
51	35.493866224748295	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	9.0
10	12.0
11	14.0
12	11.0
13	12.0
14	20.0
15	33.0
16	65.0
17	115.0
18	248.0
19	511.0
20	930.0
21	1588.0
22	2386.0
23	3538.0
24	4530.0
25	5836.0
26	6492.0
27	7874.0
28	9300.0
29	11978.0
30	15369.0
31	19893.0
32	27053.0
33	38406.0
34	69828.0
35	98109.0
36	86580.0
37	137838.0
38	257873.0
39	351847.0
40	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61989409495203	22.230047860105216	25.996452569297894	11.15360547564486
2	29.9132994126456	26.95589879033476	25.20574666511305	17.925055131906593
3	30.220484470280557	26.0830236883785	27.17936103091271	16.517130810428238
4	28.630182507109964	25.1046750993669	27.98465369394563	18.280488699577504
5	25.63100678845316	29.83941618439735	24.310861956610864	20.218715070538632
6	25.101999421708378	34.07501391783943	24.733014841379784	16.089971819072403
7	78.28963779092601	5.622634506747455	10.440753160104784	5.646974542221762
8	76.39180552139032	6.066192812784561	9.39352744943185	8.148474216393273
9	71.0739393311669	7.425350751131768	11.841513570432898	9.659196347268436
10	38.41720719670978	28.47085021815404	18.373705856713148	14.738236728423033
11	27.761105141185155	23.878351609937983	26.789575214593665	21.5709680342832
12	25.12901513484121	21.43166017167493	31.59440179184091	21.844922901642953
13	22.57624602424509	26.179779644997993	33.010007897564705	18.233966433192215
14	18.167937613554468	29.49701575628892	29.181372104765728	23.153674525390887
15	15.606364660339983	25.56195704242675	37.5911132976864	21.24056499954686
16	17.247418186839983	27.791918590349436	31.553144568590135	23.40751865422045
17	17.773749875926235	26.447606347397905	28.489062088668504	27.289581688007353
18	18.212302075376428	25.846700932603135	32.32184086622906	23.619156125791374
19	19.246580958669412	27.717258552458386	29.004604754938136	24.031555733934066
20	22.13562233241411	27.57622444619946	29.771920057656537	20.516233163729893
21	20.72070672415058	28.760513902734804	29.904754506574832	20.614024866539786
22	19.01664530440149	25.336941182563212	27.796579448206217	27.849834064829082
23	18.1403177151439	27.815654440546012	28.951522762680337	25.092505081629746
24	20.64535618879927	24.970632279893145	29.35624058657759	25.027770944729994
25	17.89484586802004	28.92165874752392	26.388137253632664	26.795358130823377
26	17.522581424755195	28.11205047536435	27.975159353867003	26.390208746013457
27	20.881851568939698	29.656865918340042	26.551957776080304	22.909324736639952
28	18.441288295636486	26.70662920717945	29.29694411717742	25.555138380006646
29	20.415679471078946	24.12468657888718	28.05983160493188	27.399802345101996
30	21.053008626902646	30.209350198733798	25.08862103341576	23.649020140947794
31	23.03482265004294	27.870548988637	23.378345136524295	25.716283224795767
32	23.938942762076152	27.901017189071155	23.496420202229444	24.663619846623252
33	21.3698606489813	29.16825265302071	23.081431228610764	26.380455469387226
34	21.4663576690532	24.214796497451633	26.00870889921758	28.31013693427759
35	19.061613951501187	25.038473655364086	27.08864692707052	28.811265466064206
36	21.955920368380394	26.912052201607995	24.63116646599084	26.500860964020767
37	21.873405921878845	25.7425221282858	28.450307918711186	23.93376403112417
38	21.933565513104348	26.791128833879256	25.834530914865976	25.440774738150417
39	22.51720849139252	24.559441042305917	26.960818584739144	25.962531881562423
40	20.445543486235366	23.990643759413423	29.753190314046872	25.810622440304336
41	18.136347354747386	24.827008808158226	26.74210351420051	30.294540322893877
42	21.139493433800713	22.15694144149976	29.625016722985364	27.07854840171416
43	24.027499061355016	22.633471001264475	27.088215366157858	26.25081457122265
44	21.985525446989215	23.758205051852045	26.888230039228887	27.368039461929854
45	22.304448961448664	23.04405805357397	25.407889796605343	29.243603188372024
46	24.21410599999137	24.7293897297134	26.889006848871684	24.167497421423548
47	18.16353569224528	23.8213855694662	31.656028690169475	26.35905004811904
48	20.578118998606058	22.208556126654496	28.757406664163614	28.45591821057583
49	21.46817022488639	20.582952480827906	32.274369165835914	25.67450812844979
50	21.188691377844528	20.845773076640903	30.20762395508314	27.757911590431434
51	18.97469758369045	20.920260490166882	27.141297358415656	32.96374456772701
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1033.0
1	1177.5
2	1322.0
3	1850.0
4	2378.0
5	1834.0
6	1290.0
7	1382.0
8	1474.0
9	1646.0
10	1818.0
11	2045.5
12	2273.0
13	2321.0
14	2369.0
15	2348.0
16	2327.0
17	2436.0
18	2545.0
19	2322.5
20	2100.0
21	2661.0
22	3222.0
23	3523.5
24	3825.0
25	4148.0
26	5979.5
27	7488.0
28	9675.0
29	11862.0
30	13862.0
31	15862.0
32	17595.0
33	19328.0
34	21706.5
35	24085.0
36	27698.0
37	31311.0
38	33010.5
39	34710.0
40	39782.0
41	44854.0
42	51399.0
43	57944.0
44	65606.5
45	73269.0
46	77788.5
47	82308.0
48	109514.5
49	136721.0
50	148008.5
51	159296.0
52	154162.0
53	149028.0
54	120103.0
55	91178.0
56	76730.0
57	62282.0
58	49421.0
59	36560.0
60	31338.0
61	26116.0
62	23775.0
63	21434.0
64	17677.5
65	13921.0
66	11233.5
67	8546.0
68	7421.0
69	6296.0
70	5271.5
71	4247.0
72	3600.5
73	2954.0
74	2472.0
75	1572.0
76	1154.0
77	888.5
78	623.0
79	450.0
80	277.0
81	257.0
82	237.0
83	199.0
84	161.0
85	116.0
86	71.0
87	41.0
88	11.0
89	8.5
90	6.0
91	6.5
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1158585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.639865972799072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.0063571506926	13.210826329974045
2	12.604357905749172	5.203045156957066
3	6.26051732986571	3.8764871582644247
4	3.6937229148085495	3.0495178360922073
5	2.4420314134999654	2.5201600538002173
6	1.6863634793931608	2.0883789717658767
7	1.2271967042588832	1.7730422848714857
8	0.9284495716635073	1.5330459777309995
9	0.7123911406313608	1.3233291896556665
>10	4.494730758053601	18.361566194338348
>50	1.1317466600372983	17.2866060906806
>100	0.7935332333246911	23.315120928646945
>500	0.010991936103591877	1.5686459712506398
>1k	0.005495968051795939	2.0139260731192503
>5k	0.002113833866075361	2.8763017828522464
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	9329	0.8052063508503907	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	7240	0.6249002015389462	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	5771	0.49810760539796384	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	5436	0.4691930242494076	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	5187	0.4477012907986898	TruSeq Adapter, Index 8 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	4696	0.40532200917498495	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3289	0.2838807683510489	TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2501	0.21586676851504208	TruSeq Adapter, Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1380	0.11911081189554501	TruSeq Adapter, Index 13 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1379	0.119024499713012	TruSeq Adapter, Index 13 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1360	0.11738456824488491	TruSeq Adapter, Index 8 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1331	0.11488151495142782	TruSeq Adapter, Index 8 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1330	0.11479520276889482	TruSeq Adapter, Index 8 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC	1222	0.10547348705533043	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	1207	0.10417880431733537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4696245851620727	0.0
2	0.0	0.0	0.0	2.4999460548859167	0.0
3	0.0	0.0	0.0	3.4016494258082055	0.0
4	0.0	0.0	0.0	4.557024301194992	0.0
5	0.0	0.0	0.0	7.79122809288917	0.0
6	0.0	0.0	0.0	9.17627968599628	0.0
7	0.0	0.0	0.0	10.784103022221071	0.0
8	0.0	0.0	0.0	13.203778747351295	0.0
9	0.0	0.0	0.0	14.044545717405283	0.0
10	0.0	0.0	0.0	16.331818554529878	0.0
11	0.0	0.0	0.0	19.11063927117993	0.0
12	0.0	0.0	0.0	21.838363175770443	0.0
13	0.0	0.0	0.0	22.69259484629958	0.0
14	0.0	0.0	0.0	23.028867109448164	0.0
15	8.631218253300361E-5	0.0	0.0	23.705986181419576	0.0
16	8.631218253300361E-5	0.0	0.0	24.94974473172016	0.0
17	8.631218253300361E-5	0.0	0.0	26.428099794145446	0.0
18	1.7262436506600723E-4	0.0	0.0	28.060953663304808	0.0
19	1.7262436506600723E-4	0.0	0.0	29.14063275461015	0.0
20	1.7262436506600723E-4	0.0	0.0	30.037157394580458	0.0
21	1.7262436506600723E-4	0.0	0.0	31.15610852893832	0.0
22	1.7262436506600723E-4	0.0	0.0	32.25089225218694	0.0
23	1.7262436506600723E-4	0.0	0.0	33.29380235373322	0.0
24	2.5893654759901087E-4	0.0	0.0	34.12878640755749	0.0
25	2.5893654759901087E-4	0.0	0.0	34.84888894643034	0.0
26	2.5893654759901087E-4	0.0	0.0	35.55552678482805	0.0
27	2.5893654759901087E-4	0.0	0.0	36.160402560019335	0.0
28	3.4524873013201446E-4	0.0	0.0	36.773391680368725	0.0
29	3.4524873013201446E-4	0.0	0.0	37.444900460475495	0.0
30	3.4524873013201446E-4	0.0	0.0	38.149466806492406	0.0
31	4.315609126650181E-4	0.0	0.0	38.83668440382018	0.0
32	4.315609126650181E-4	0.0	0.0	39.42006844556075	0.0
33	4.315609126650181E-4	0.0	0.0	40.00250305329346	0.0
34	4.315609126650181E-4	0.0	0.0	40.59857498586638	0.0
35	4.315609126650181E-4	0.0	0.0	41.23305583966649	0.0
36	4.315609126650181E-4	0.0	0.0	41.83102664025514	0.0
37	4.315609126650181E-4	0.0	0.0	42.407764643940666	0.0
38	4.315609126650181E-4	0.0	0.0	42.98277640397554	0.0
39	4.315609126650181E-4	0.0	0.0	43.55260943305843	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	20	7.0333085E-4	45.000004	16
TGTTTCG	20	7.0333085E-4	45.000004	12
TCGCAAA	20	7.0333085E-4	45.000004	31
AGATCGC	20	7.0333085E-4	45.000004	22
GCGACTT	35	1.211938E-7	45.000004	42
ATAGGCC	20	7.0333085E-4	45.000004	25
CTATCGG	85	0.0	45.000004	2
GTTTACG	40	6.8157533E-9	45.000004	1
GCAACGA	65	0.0	45.000004	11
ATTACGC	35	1.211938E-7	45.000004	30
GAATCGT	65	0.0	45.000004	15
GAATCGC	20	7.0333085E-4	45.000004	7
CAACCCG	20	7.0333085E-4	45.000004	30
CTAAGTC	20	7.0333085E-4	45.000004	22
CGTTCGG	20	7.0333085E-4	45.000004	45
TACGCTT	35	1.211938E-7	45.000004	26
CCCGTCG	20	7.0333085E-4	45.000004	43
CCGTACG	35	1.211938E-7	45.000004	24
TACGAAC	20	7.0333085E-4	45.000004	42
CGAATAT	130	0.0	45.000004	14
>>END_MODULE