##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934969.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1720734
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.609731079876376	34.0	31.0	34.0	31.0	34.0
2	32.77051711653283	34.0	31.0	34.0	31.0	34.0
3	32.818926109439346	34.0	31.0	34.0	31.0	34.0
4	36.21786807257833	37.0	37.0	37.0	35.0	37.0
5	36.111636080881766	37.0	35.0	37.0	35.0	37.0
6	36.10662659074558	37.0	36.0	37.0	35.0	37.0
7	36.29281864599642	37.0	37.0	37.0	35.0	37.0
8	36.31846002926658	37.0	37.0	37.0	35.0	37.0
9	38.14590517767418	39.0	39.0	39.0	37.0	39.0
10	37.6254650631649	39.0	38.0	39.0	35.0	39.0
11	37.44434351852175	39.0	37.0	39.0	34.0	39.0
12	37.51088779555701	39.0	37.0	39.0	35.0	39.0
13	37.5523108161982	39.0	37.0	39.0	35.0	39.0
14	38.88760958986107	40.0	38.0	41.0	35.0	41.0
15	38.932877481353884	40.0	38.0	41.0	35.0	41.0
16	39.010239235117105	40.0	38.0	41.0	35.0	41.0
17	39.017597722832235	40.0	38.0	41.0	35.0	41.0
18	38.486889315838475	39.0	38.0	41.0	35.0	41.0
19	37.943412520470915	38.0	37.0	41.0	35.0	41.0
20	37.36266325881862	38.0	35.0	41.0	34.0	41.0
21	37.325527362160564	38.0	35.0	41.0	34.0	41.0
22	37.1437328488889	38.0	35.0	41.0	34.0	41.0
23	37.03543371607697	38.0	35.0	40.0	34.0	41.0
24	36.83042701544806	38.0	35.0	40.0	33.0	41.0
25	36.689211696868895	37.0	35.0	40.0	33.0	41.0
26	36.80441427902279	38.0	35.0	40.0	33.0	41.0
27	36.85174175671545	38.0	35.0	40.0	33.0	41.0
28	36.78490690600639	38.0	35.0	40.0	33.0	41.0
29	36.63156013654638	38.0	35.0	40.0	33.0	41.0
30	36.314372238823665	38.0	35.0	40.0	33.0	41.0
31	35.74057814862727	38.0	35.0	40.0	30.0	41.0
32	34.921188864751905	38.0	35.0	40.0	21.0	41.0
33	33.86409869276716	38.0	34.0	41.0	15.0	41.0
34	32.97612937269793	38.0	33.0	41.0	10.0	41.0
35	32.417364334057446	38.0	31.0	41.0	8.0	41.0
36	32.17819198086398	38.0	31.0	41.0	7.0	41.0
37	31.984240446228178	38.0	30.0	41.0	7.0	41.0
38	31.85891602072139	38.0	29.0	41.0	7.0	41.0
39	31.793183025383353	38.0	29.0	41.0	7.0	41.0
40	31.6857323676989	38.0	27.0	41.0	7.0	41.0
41	31.551718627051013	38.0	27.0	41.0	7.0	41.0
42	31.42283060600883	38.0	25.0	40.0	7.0	41.0
43	31.323375373532457	37.0	24.0	40.0	7.0	41.0
44	31.20839246507595	37.0	24.0	40.0	7.0	41.0
45	31.16226621895075	37.0	23.0	40.0	7.0	41.0
46	31.08876735160693	37.0	23.0	40.0	7.0	41.0
47	31.055054993973503	37.0	23.0	40.0	7.0	41.0
48	30.982774211470222	37.0	23.0	40.0	7.0	41.0
49	30.845071347459864	36.0	24.0	40.0	7.0	41.0
50	30.755470049409148	36.0	24.0	40.0	7.0	41.0
51	30.205209521053224	35.0	23.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	8.0
10	10.0
11	10.0
12	17.0
13	20.0
14	25.0
15	44.0
16	115.0
17	237.0
18	534.0
19	1070.0
20	1903.0
21	3179.0
22	5316.0
23	9800.0
24	20642.0
25	45832.0
26	80756.0
27	93354.0
28	82258.0
29	62728.0
30	48161.0
31	40812.0
32	39006.0
33	45725.0
34	74389.0
35	97762.0
36	106428.0
37	145732.0
38	291388.0
39	423161.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.118736539174563	15.158066267069751	18.799651776509325	36.92354541724636
2	52.1917972214183	16.903774784481506	18.844923154886228	12.059504839213965
3	22.5558976576275	17.37456225076043	47.41435922112308	12.655180870488989
4	19.89546321511634	19.195122546541185	47.473694365311545	13.435719873030926
5	18.557836365179046	21.934651143058716	46.11433260457456	13.393179887187676
6	19.33738741723009	25.114631314311218	44.93303439113773	10.614946877320957
7	60.71850733466068	3.3474087220918514	32.77984859949301	3.1542353437544675
8	62.00609739797086	2.8530266735009593	32.259954182343115	2.8809217461850585
9	58.20057022177745	4.849384041926294	33.403477818186886	3.5465679181093646
10	27.901174731248407	23.314411175695955	39.43032450105595	9.354089591999692
11	18.967312786287714	18.755891381236147	48.2315105065629	14.045285325913243
12	18.85515134820373	16.45681435945358	49.98657549627078	14.70145879607191
13	16.88413200413312	18.099194878464655	50.87084930035671	14.145823817045516
14	14.348644241352817	20.775087840421588	48.99415017079921	15.882117747426388
15	13.526088285580457	20.607601174847478	51.54509645302527	14.321214086546789
16	15.771699751385166	19.387191744918155	50.43708092011897	14.404027583577705
17	15.868693243697166	19.55380668947089	48.36261734817816	16.214882718653783
18	15.777394995391504	19.429266812883338	49.95153231121138	14.841805880513782
19	16.241034349295127	20.929033772796956	48.067336380870024	14.762595497037893
20	17.20475099579598	20.444240655441224	48.8032432671174	13.547765081645391
21	16.844207181354005	20.026105138853538	48.96887026117924	14.16081741861322
22	15.7743149144493	18.64355559894789	48.37261308255663	17.20951640404618
23	14.649387993728258	20.207423111300177	49.00141451264402	16.141774382327544
24	15.001156483221695	19.118992244007497	50.10716357089474	15.772687701876059
25	14.721217805889811	21.328107656383846	47.540526310283866	16.410148227442477
26	13.943119622207734	21.851721416558284	48.22163100165395	15.98352795958004
27	14.572618429112227	21.278535787634812	48.24662033760011	15.902225445652842
28	13.722632318533833	20.752016290722448	49.324648667371015	16.2007027233727
29	15.057179087528926	19.656262966850193	48.35698021890658	16.9295777267143
30	15.607816199366084	20.19673000010461	47.11535891079039	17.080094889738913
31	16.081625631852454	21.51558579071489	45.738853303299635	16.663935274133017
32	16.67561633581948	22.769817996273684	43.727269874367565	16.827295793539268
33	16.33558702274727	23.25368127787328	41.78280896408161	18.627922735297844
34	16.313096620395715	23.9871473452608	40.87488246294895	18.824873571394534
35	16.057624246397175	25.537416009679593	38.88067533970968	19.524284404213553
36	17.026803677965333	27.277487397819765	36.66749189590024	19.02821702831466
37	17.867317086778083	26.31667648805684	37.856984286938015	17.95902213822706
38	17.959951973983195	26.200214559600727	36.683531562693595	19.15630190372248
39	17.94664369972349	25.59198574561786	37.16257132130823	19.29879923335042
40	17.65316428919287	25.390037042331937	37.99006702953507	18.966731638940125
41	16.23847730096575	26.414425471920705	36.97701097322422	20.370086253889326
42	17.670831168559463	26.466496274264355	36.14445928307339	19.718213274102794
43	18.336244881544737	26.371827371342693	35.07073144367462	20.221196303437953
44	17.919910921734562	25.826536815103324	34.92126034587565	21.33229191728646
45	18.094080781805904	24.86590024954467	35.1573805131996	21.882638455449825
46	17.789385227466884	25.610117542862522	35.85423429768924	20.746262931981352
47	16.23016689389528	25.0335031445883	38.59940002347835	20.13692993803807
48	16.992864672866347	24.32223690587854	37.64817804495058	21.03672037630453
49	17.67606149468773	23.722550957905174	37.63364936126095	20.967738186146146
50	16.949975998614544	23.267861273154363	37.859076417389325	21.923086310841768
51	16.480583285969825	23.16645106100071	36.50779260478377	23.84517304824569
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	271.0
1	663.0
2	1055.0
3	62911.5
4	124768.0
5	87631.5
6	50495.0
7	48480.5
8	46466.0
9	46047.5
10	45629.0
11	44730.0
12	43831.0
13	42117.0
14	40403.0
15	37340.0
16	34277.0
17	31698.0
18	29119.0
19	26754.0
20	24389.0
21	22674.5
22	20960.0
23	19568.0
24	18176.0
25	18329.5
26	19799.0
27	21115.0
28	21898.5
29	22682.0
30	24402.0
31	26122.0
32	28300.5
33	30479.0
34	36685.0
35	42891.0
36	45909.5
37	48928.0
38	53180.0
39	57432.0
40	61352.0
41	65272.0
42	72849.5
43	80427.0
44	87286.0
45	94145.0
46	105744.0
47	117343.0
48	124480.0
49	131617.0
50	128235.0
51	124853.0
52	108721.0
53	92589.0
54	80071.5
55	67554.0
56	60488.5
57	53423.0
58	47354.0
59	41285.0
60	38274.0
61	35263.0
62	31618.0
63	27973.0
64	23775.0
65	19577.0
66	14127.0
67	8677.0
68	6982.5
69	5288.0
70	4120.5
71	2953.0
72	2402.0
73	1851.0
74	1408.0
75	888.0
76	811.0
77	652.5
78	494.0
79	377.0
80	260.0
81	155.5
82	51.0
83	46.0
84	41.0
85	27.5
86	14.0
87	23.5
88	33.0
89	18.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1720734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.450846189244345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00297019524317	27.652712986957766
2	9.922693081806951	7.0353573245243455
3	3.6595713425193312	3.892047023466577
4	1.8529319960350148	2.6275202876226733
5	1.0634619670069294	1.8850313310236948
6	0.6888760331166027	1.4652742976084085
7	0.4860739010926947	1.2062211772970164
8	0.3680423709090151	1.043793078577625
9	0.27842604524276404	0.8883395014482733
>10	2.8387483582647266	24.03993534812355
>50	0.7554895754823961	17.692553133953982
>100	0.07701065096456984	4.788840987421802
>500	0.003187798941211993	0.7209454052259537
>1k	0.0023489044829983104	1.2887917696919369
>5k	0.0	0.0
>10k+	1.6777889164273647E-4	3.7726363470564968
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63428	3.6861013962646174	No Hit
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2859	0.16615002667466325	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2833	0.16463904357094122	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	1950	0.11332373277915123	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1751	0.10175890056220195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7434420427561728E-4	0.0	0.0	0.1296539732463007	0.0
2	1.7434420427561728E-4	0.0	0.0	0.6602415015917625	0.0
3	1.7434420427561728E-4	0.0	0.0	0.9942849969838453	0.0
4	1.7434420427561728E-4	0.0	0.0	1.351516271544585	0.0
5	2.3245893903415634E-4	0.0	0.0	2.262987771497512	0.0
6	2.3245893903415634E-4	0.0	0.0	2.7271501580139637	0.0
7	2.3245893903415634E-4	0.0	0.0	3.2227526160347852	0.0
8	2.3245893903415634E-4	0.0	0.0	4.0236898904769705	0.0
9	2.3245893903415634E-4	0.0	0.0	4.294330210247487	0.0
10	2.3245893903415634E-4	0.0	0.0	5.0397097982605095	0.0
11	2.3245893903415634E-4	0.0	0.0	6.1941008895041305	0.0
12	2.3245893903415634E-4	0.0	0.0	7.084941658617776	0.0
13	2.9057367379269544E-4	0.0	0.0	7.399632947335265	0.0
14	2.9057367379269544E-4	0.0	0.0	7.520685939837302	0.0
15	2.9057367379269544E-4	0.0	0.0	7.75628307454842	0.0
16	2.9057367379269544E-4	0.0	0.0	8.310581414675365	0.0
17	2.9057367379269544E-4	0.0	0.0	9.067293375966303	0.0
18	2.9057367379269544E-4	0.0	0.0	9.904668589102092	0.0
19	2.9057367379269544E-4	0.0	0.0	10.423575055761088	0.0
20	2.9057367379269544E-4	0.0	0.0	10.917375957004394	0.0
21	2.9057367379269544E-4	0.0	0.0	11.57029500201658	0.0
22	2.9057367379269544E-4	0.0	0.0	12.277667553497519	0.0
23	3.4868840855123456E-4	0.0	0.0	13.047106641700577	0.0
24	4.068031433097736E-4	0.0	0.0	13.597336950394425	0.0
25	4.068031433097736E-4	0.0	0.0	14.092067687393868	0.0
26	4.068031433097736E-4	0.0	0.0	14.560123761139142	0.0
27	4.068031433097736E-4	0.0	0.0	15.02126418144815	0.0
28	4.649178780683127E-4	0.0	0.0	15.489727058336733	0.0
29	4.649178780683127E-4	0.0	0.0	16.013515162715446	0.0
30	4.649178780683127E-4	0.0	0.0	16.551541377110002	0.0
31	5.230326128268518E-4	0.0	0.0	17.083872347498218	0.0
32	5.811473475853909E-4	0.0	0.0	17.581102018092277	0.0
33	5.811473475853909E-4	0.0	0.0	18.05839833466416	0.0
34	6.3926208234393E-4	0.0	0.0	18.514657117253453	0.0
35	6.3926208234393E-4	0.0	0.0	19.031529568195896	0.0
36	6.3926208234393E-4	0.0	0.0	19.50278195235289	0.0
37	6.973768171024691E-4	0.0	0.0	19.989027938077587	0.0
38	6.973768171024691E-4	0.0	0.0	20.464115894728646	0.0
39	6.973768171024691E-4	0.0	0.0	20.953848764538854	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAA	35	1.2123928E-7	45.000004	9
CTCCGTA	20	7.0342654E-4	45.0	30
ATTACGC	20	7.0342654E-4	45.0	35
CCGCTAA	20	7.0342654E-4	45.0	22
GTAATCG	20	7.0342654E-4	45.0	1
TGTAGCG	45	3.8562575E-10	45.0	1
CGTTTTT	49450	0.0	44.558647	1
TCGTAAG	65	0.0	41.538464	1
CGCGAAG	60	3.6379788E-12	41.249996	1
TGCGAAG	115	0.0	41.08696	1
GCGTTAG	110	0.0	40.909092	1
TAGCGAG	100	0.0	40.5	1
CCTCGTG	95	0.0	40.263157	15
CGACATA	45	1.9292202E-8	40.0	34
CACTCGA	40	3.459554E-7	39.375	44
TGTAACG	80	0.0	39.375	1
TAGTCCG	40	3.459554E-7	39.375	1
TCGCGCG	40	3.459554E-7	39.375	1
AGTCGTG	40	3.459554E-7	39.375	1
GTTGCCG	40	3.459554E-7	39.375	1
>>END_MODULE