##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934967.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1714504
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70302198186764	34.0	31.0	34.0	31.0	34.0
2	32.84886999388745	34.0	31.0	34.0	31.0	34.0
3	32.89055493600482	34.0	31.0	34.0	31.0	34.0
4	36.26445957548072	37.0	37.0	37.0	35.0	37.0
5	36.16224575737356	37.0	37.0	37.0	35.0	37.0
6	36.166570623340625	37.0	37.0	37.0	35.0	37.0
7	36.30046065800955	37.0	37.0	37.0	35.0	37.0
8	36.31134427216268	37.0	37.0	37.0	35.0	37.0
9	38.0913640329798	39.0	39.0	39.0	37.0	39.0
10	37.68951428518102	39.0	38.0	39.0	35.0	39.0
11	37.58202663860802	39.0	37.0	39.0	35.0	39.0
12	37.49984310331151	39.0	37.0	39.0	35.0	39.0
13	37.46056235506012	39.0	37.0	39.0	35.0	39.0
14	38.79594448306916	40.0	38.0	41.0	35.0	41.0
15	38.835248561683144	40.0	38.0	41.0	35.0	41.0
16	38.913447854306554	40.0	38.0	41.0	35.0	41.0
17	38.910558972157546	40.0	38.0	41.0	35.0	41.0
18	38.3785473816334	39.0	38.0	41.0	35.0	41.0
19	37.89305011828494	38.0	37.0	41.0	35.0	41.0
20	37.3214894803395	38.0	35.0	41.0	34.0	41.0
21	37.26339921050053	38.0	35.0	41.0	34.0	41.0
22	37.077904746795575	38.0	35.0	41.0	34.0	41.0
23	36.97897409396537	38.0	35.0	40.0	34.0	41.0
24	36.78782551688418	37.0	35.0	40.0	33.0	41.0
25	36.64524608866471	37.0	35.0	40.0	33.0	41.0
26	36.733966791561876	37.0	35.0	40.0	33.0	41.0
27	36.7520688199036	37.0	35.0	40.0	33.0	41.0
28	36.65932246293972	37.0	35.0	40.0	33.0	41.0
29	36.474881948365244	37.0	35.0	40.0	33.0	41.0
30	36.15810870082543	37.0	35.0	40.0	32.0	41.0
31	35.59528411715575	37.0	35.0	40.0	30.0	41.0
32	34.842275666898416	37.0	35.0	40.0	22.0	41.0
33	33.90317316261729	37.0	34.0	40.0	16.0	41.0
34	33.11316334053464	37.0	33.0	41.0	12.0	41.0
35	32.62597987522922	37.0	33.0	41.0	10.0	41.0
36	32.37758908698959	37.0	32.0	40.0	8.0	41.0
37	32.14379494011096	37.0	31.0	40.0	8.0	41.0
38	32.00288829888994	37.0	30.0	40.0	8.0	41.0
39	31.929862514173195	37.0	30.0	40.0	7.0	41.0
40	31.816591853970593	37.0	29.0	40.0	7.0	41.0
41	31.666092934166382	36.0	29.0	40.0	7.0	41.0
42	31.518821770027948	36.0	29.0	40.0	7.0	41.0
43	31.39764095038565	36.0	27.0	40.0	7.0	41.0
44	31.293567119120166	36.0	26.0	40.0	7.0	41.0
45	31.245189862490843	36.0	25.0	40.0	7.0	41.0
46	31.17961171277524	35.0	25.0	40.0	7.0	41.0
47	31.161375534848563	35.0	25.0	40.0	7.0	41.0
48	31.10358331039181	35.0	25.0	40.0	7.0	41.0
49	30.976411253633703	35.0	25.0	40.0	7.0	41.0
50	30.90861380317573	35.0	25.0	40.0	7.0	41.0
51	30.377872550895187	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	18.0
11	19.0
12	28.0
13	28.0
14	46.0
15	90.0
16	164.0
17	335.0
18	609.0
19	1088.0
20	1915.0
21	3079.0
22	4991.0
23	8677.0
24	17458.0
25	38094.0
26	69258.0
27	87705.0
28	83588.0
29	65935.0
30	51754.0
31	44232.0
32	42318.0
33	49424.0
34	91373.0
35	116429.0
36	114847.0
37	146111.0
38	274150.0
39	400433.0
40	299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.725856574262878	14.590575466723903	17.291823174515777	38.39174478449744
2	52.44764666632449	17.28762370924769	17.41150793465632	12.853221689771502
3	23.688133710974135	17.35277374680957	45.9933601788039	12.96573236341239
4	21.183910915343446	18.759652937525956	45.92646036404698	14.129975783083621
5	19.113749515894977	21.85121761162412	44.68551837732662	14.349514495154283
6	20.57014740123091	24.704112676319216	43.572193474030975	11.1535464484189
7	59.494990971149676	4.220287616710139	32.789074857801445	3.495646554338747
8	60.277491332770296	3.754846882830253	32.40797338472235	3.559688399677108
9	56.06163648495425	5.346444219436059	34.0489144382282	4.543004857381494
10	31.27388445871226	19.298759291316905	38.93965835017008	10.487697899800759
11	24.40250941380131	18.299753164763686	43.407597765884475	13.890139655550527
12	23.25558878836095	15.73953749889181	46.96139524900496	14.043478463742284
13	19.26218894794063	17.10605516230933	47.3682767727576	16.263479116992436
14	14.807197883469506	19.84177348084344	47.680203720726226	17.67082491496083
15	14.545081259652937	19.46358830308941	51.051382790591326	14.939947646666324
16	16.918712350627356	18.45064228488239	49.63563806208676	14.995007302403494
17	16.66796927857853	18.134865827084685	47.22736138265061	17.969803511686177
18	16.43087446865099	18.98502424024674	47.88661910383411	16.697482187268154
19	18.25944996337133	19.824217382986568	46.10388777162375	15.812444882018356
20	20.252737818051166	18.980941426791656	46.52377597252617	14.242544782631011
21	19.304883511499536	19.142970795052097	46.496304470564084	15.055841222884286
22	17.631454928072493	18.68260441503782	46.23745409751158	17.448486559378107
23	17.414657533607386	18.763210817822532	47.400122717707276	16.422008930862802
24	17.670183329989314	17.503137933769768	47.98221526458965	16.844463471651277
25	16.76869811910617	18.980999752698157	46.12873460779328	18.1215675204024
26	15.455490334230776	20.792077475468123	46.36250484105024	17.38992734925086
27	16.65764559312781	20.476301017670416	46.75958761251067	16.1064657766911
28	15.160419573241008	20.506922118583567	47.41330437257656	16.919353935598867
29	16.62859929169019	20.15480862103559	46.3983752735485	16.81821681372572
30	17.753939331725093	19.704124341500513	45.30762249606883	17.234313830705556
31	16.997160694871518	21.089189643185435	44.3690420086509	17.544607653292147
32	19.701849631146967	22.028878322826895	41.96508144629584	16.3041905997303
33	19.599954272489303	22.915898708897732	39.82726199530593	17.65688502330703
34	17.811507001441818	23.821641710955472	40.086637301516944	18.280213986085773
35	19.392897304409907	25.173927853186694	36.661856723577195	18.7713181188262
36	19.050582559154133	27.960214732657374	35.191693924306975	17.79750878388152
37	19.84982245594061	27.14837644006663	36.14654733963875	16.855253764354007
38	19.635708053174564	26.579407222147044	34.62873227475701	19.156152449921375
39	19.559126137938435	26.396905460704673	35.06885956521536	18.975108836141533
40	19.869653264151033	25.14085706420049	36.419279278438545	18.570210393209933
41	17.98875943129908	27.109764689962812	35.107646293038684	19.79382958569942
42	18.87513823239841	27.308597705225534	34.101057798640305	19.71520626373575
43	19.339295796335268	26.716998035583465	34.19356268635127	19.750143481729992
44	18.701793638276726	26.26864679230845	34.43456533201439	20.59499423740044
45	18.985432521592248	25.510934940950854	33.96918292404101	21.534449613415894
46	18.34880525213123	26.3889731374205	35.144099984601965	20.118121625846307
47	17.76548786121234	25.23884458712257	37.39408015379375	19.601587397871338
48	18.374060369646266	23.69641598969731	37.45905521363613	20.470468427020293
49	18.507510043721098	24.28626588214434	37.534528936648734	19.671695137485827
50	17.530317806199346	23.36710792159132	37.56724977019593	21.535324502013413
51	16.849887780955893	23.40968583333722	36.58848273319864	23.151943652508248
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	479.0
1	972.5
2	1466.0
3	72718.0
4	143970.0
5	99973.5
6	55977.0
7	53141.5
8	50306.0
9	48177.0
10	46048.0
11	43675.5
12	41303.0
13	38467.0
14	35631.0
15	32183.0
16	28735.0
17	26206.0
18	23677.0
19	21562.0
20	19447.0
21	17983.5
22	16520.0
23	15497.0
24	14474.0
25	13883.0
26	14880.5
27	16469.0
28	17123.0
29	17777.0
30	20150.5
31	22524.0
32	24826.0
33	27128.0
34	29839.0
35	32550.0
36	35461.5
37	38373.0
38	40804.0
39	43235.0
40	48895.0
41	54555.0
42	61326.5
43	68098.0
44	73414.0
45	78730.0
46	86600.0
47	94470.0
48	99756.5
49	105043.0
50	106338.5
51	107634.0
52	95957.0
53	84280.0
54	77174.5
55	70069.0
56	67393.5
57	64718.0
58	65612.0
59	66506.0
60	66316.5
61	66127.0
62	62044.5
63	57962.0
64	48126.5
65	38291.0
66	30874.5
67	23458.0
68	20234.0
69	17010.0
70	13722.5
71	10435.0
72	9064.5
73	7694.0
74	5927.5
75	3470.5
76	2780.0
77	2154.5
78	1529.0
79	1146.5
80	764.0
81	613.5
82	463.0
83	335.5
84	208.0
85	160.5
86	113.0
87	61.0
88	9.0
89	10.0
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1714504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.00893760790645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55706474853072	23.27405117091019
2	9.669552122861493	5.803459727026997
3	3.63371635299013	3.271319019651305
4	1.846608835319449	2.2165907730124053
5	1.0106405550783915	1.5164124680683693
6	0.6690104077140107	1.204577495047788
7	0.4586744287472318	0.9635032620232468
8	0.34377863158733746	0.8253145204988692
9	0.26676252550852225	0.7204733985701517
>10	3.268346240292609	25.112482419264648
>50	1.1586439716928167	23.188637576039522
>100	0.1100499224722963	5.871799322601706
>500	0.004767504803394883	0.9736356788304037
>1k	0.002185106368222655	1.1404288355907948
>5k	0.0	0.0
>10k+	1.9864603347478682E-4	3.917314332863598
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65714	3.8328286198224095	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	3337	0.19463354999463403	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	2977	0.17363622365418802	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1908	0.1112858296043637	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10516160942173364	0.0
2	0.0	0.0	0.0	0.6146967286165561	0.0
3	0.0	0.0	0.0	0.8919780881234456	0.0
4	0.0	0.0	0.0	1.171942439329392	0.0
5	0.0	0.0	0.0	1.9034076327614284	0.0
6	0.0	0.0	0.0	2.282584350925982	0.0
7	0.0	0.0	0.0	2.6780339970043814	0.0
8	0.0	0.0	0.0	3.2548772123016336	0.0
9	0.0	0.0	0.0	3.4618758544745303	0.0
10	0.0	0.0	0.0	3.990250241469253	0.0
11	0.0	0.0	0.0	4.803488355816026	0.0
12	0.0	0.0	0.0	5.477794160876848	0.0
13	5.832590650123884E-5	0.0	0.0	5.7154139039628955	0.0
14	5.832590650123884E-5	0.0	0.0	5.81730926262056	0.0
15	5.832590650123884E-5	0.0	0.0	6.033348420301149	0.0
16	5.832590650123884E-5	0.0	0.0	6.492664933998404	0.0
17	5.832590650123884E-5	0.0	0.0	7.027863452053772	0.0
18	5.832590650123884E-5	0.0	0.0	7.616021893212264	0.0
19	5.832590650123884E-5	0.0	0.0	7.967377153975727	0.0
20	5.832590650123884E-5	0.0	0.0	8.357927423908022	0.0
21	1.1665181300247768E-4	0.0	0.0	8.822084987844882	0.0
22	1.1665181300247768E-4	0.0	0.0	9.31925501486144	0.0
23	1.1665181300247768E-4	0.0	0.0	9.794436175127034	0.0
24	1.1665181300247768E-4	0.0	0.0	10.200267832562654	0.0
25	1.1665181300247768E-4	0.0	0.0	10.546432087647506	0.0
26	1.1665181300247768E-4	0.0	0.0	10.86063374596968	0.0
27	1.1665181300247768E-4	0.0	0.0	11.17413549341384	0.0
28	1.1665181300247768E-4	0.0	0.0	11.506301530938394	0.0
29	1.1665181300247768E-4	0.0	0.0	11.850424379295703	0.0
30	1.1665181300247768E-4	0.0	0.0	12.230301008338271	0.0
31	1.1665181300247768E-4	0.0	0.0	12.587897141097367	0.0
32	1.1665181300247768E-4	0.0	0.0	12.94963441321805	0.0
33	1.1665181300247768E-4	0.0	0.0	13.276026185998983	0.0
34	1.1665181300247768E-4	0.0	0.0	13.634263903729591	0.0
35	1.7497771950371652E-4	0.0	0.0	14.002067070126405	0.0
36	1.7497771950371652E-4	0.0	0.0	14.360246461950512	0.0
37	1.7497771950371652E-4	0.0	0.0	14.720233956876157	0.0
38	1.7497771950371652E-4	0.0	0.0	15.071064284481109	0.0
39	1.7497771950371652E-4	0.0	0.0	15.446041537377575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAC	30	2.166129E-6	45.000004	25
ATTACGC	20	7.0342596E-4	45.0	11
ATAGCGC	20	7.0342596E-4	45.0	36
TATATCG	25	3.891611E-5	45.0	15
TACTCGA	20	7.0342596E-4	45.0	44
TATAGCG	20	7.0342596E-4	45.0	1
ATCGCTA	25	3.891611E-5	45.0	34
CGTTTTT	48325	0.0	44.343506	1
TAGGTCG	70	0.0	41.785713	1
TCGATGG	160	0.0	40.78125	2
GTTTTTT	54155	0.0	39.81904	2
TTGGACG	80	0.0	39.375	1
TCAGACG	110	0.0	38.863636	22
CGTAAGG	215	0.0	38.720932	2
CTATCGA	30	1.1401559E-4	37.500004	45
AACGGAT	30	1.1401559E-4	37.500004	13
TCAATCG	30	1.1401559E-4	37.500004	31
CCGTACT	30	1.1401559E-4	37.500004	26
AGTAACG	60	1.5643309E-10	37.500004	1
AAATGCG	30	1.1401559E-4	37.500004	1
>>END_MODULE