##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934966.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137440
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56917287944859	34.0	31.0	34.0	31.0	34.0
2	32.72980113236742	34.0	31.0	34.0	31.0	34.0
3	32.773957307638206	34.0	31.0	34.0	31.0	34.0
4	36.18558253622169	37.0	37.0	37.0	35.0	37.0
5	36.07480482486989	37.0	35.0	37.0	35.0	37.0
6	36.064497467998315	37.0	35.0	37.0	35.0	37.0
7	36.23506910254607	37.0	37.0	37.0	35.0	37.0
8	36.2318197003798	37.0	37.0	37.0	35.0	37.0
9	38.0221216064144	39.0	39.0	39.0	35.0	39.0
10	37.55307268954846	39.0	37.0	39.0	35.0	39.0
11	37.43787276691518	39.0	37.0	39.0	34.0	39.0
12	37.38830971303981	39.0	37.0	39.0	35.0	39.0
13	37.34949535799691	39.0	37.0	39.0	34.0	39.0
14	38.63376441834295	40.0	38.0	41.0	34.0	41.0
15	38.681562104374734	40.0	38.0	41.0	35.0	41.0
16	38.779154065269374	40.0	38.0	41.0	35.0	41.0
17	38.80509829089886	40.0	38.0	41.0	35.0	41.0
18	38.31561049374033	39.0	38.0	41.0	35.0	41.0
19	37.83863412575609	38.0	37.0	41.0	35.0	41.0
20	37.29990241243494	38.0	35.0	41.0	34.0	41.0
21	37.24677609368406	38.0	35.0	41.0	34.0	41.0
22	37.05425780700521	38.0	35.0	40.0	34.0	41.0
23	36.930590624560416	38.0	35.0	40.0	33.0	41.0
24	36.73934537206358	37.0	35.0	40.0	33.0	41.0
25	36.59581955971304	37.0	35.0	40.0	33.0	41.0
26	36.718251512167676	37.0	35.0	40.0	33.0	41.0
27	36.745896926431286	38.0	35.0	40.0	33.0	41.0
28	36.666865944577296	38.0	35.0	40.0	33.0	41.0
29	36.52431688704459	38.0	35.0	40.0	33.0	41.0
30	36.20453826135884	38.0	35.0	40.0	32.0	41.0
31	35.6521522014348	38.0	35.0	40.0	30.0	41.0
32	34.92154487269658	38.0	35.0	40.0	23.0	41.0
33	34.033880468420314	38.0	34.0	40.0	17.0	41.0
34	33.29793571529048	38.0	33.0	40.0	12.0	41.0
35	32.8220398438599	37.0	33.0	40.0	10.0	41.0
36	32.59805704037136	37.0	32.0	40.0	10.0	41.0
37	32.35206076804051	37.0	31.0	40.0	8.0	41.0
38	32.19030893937263	37.0	31.0	40.0	8.0	41.0
39	32.15301642284428	37.0	31.0	40.0	8.0	41.0
40	32.02173213532142	37.0	31.0	40.0	8.0	41.0
41	31.861897770431845	37.0	30.0	40.0	8.0	41.0
42	31.73197443381629	37.0	29.0	40.0	7.0	41.0
43	31.62498153748769	36.0	29.0	40.0	7.0	41.0
44	31.507109825573217	36.0	29.0	40.0	7.0	41.0
45	31.4817247503165	36.0	28.0	40.0	7.0	41.0
46	31.40842769728513	36.0	28.0	40.0	7.0	41.0
47	31.37589850893234	35.0	28.0	40.0	7.0	41.0
48	31.302592664228442	35.0	28.0	40.0	7.0	41.0
49	31.17729726403151	35.0	27.0	40.0	7.0	41.0
50	31.070563722042483	35.0	27.0	40.0	7.0	41.0
51	30.435842769728513	35.0	25.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	11.0
11	7.0
12	13.0
13	22.0
14	27.0
15	47.0
16	98.0
17	207.0
18	363.0
19	731.0
20	1240.0
21	2088.0
22	3233.0
23	5811.0
24	11538.0
25	24696.0
26	44307.0
27	56772.0
28	52297.0
29	41109.0
30	32323.0
31	28439.0
32	28192.0
33	35395.0
34	63141.0
35	81546.0
36	82065.0
37	108316.0
38	192539.0
39	240686.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.861320157546775	15.125457166971445	17.678207202138136	36.33501547334365
2	51.99131382754255	17.247239414826275	17.38729075819384	13.374155999437331
3	23.902095934730625	17.728583485722325	45.712477141651426	12.656843437895626
4	21.69099029399353	18.70287663525109	44.81678154452103	14.789351526234352
5	19.140789843859896	21.865504993669997	43.76485792657195	15.228847235898158
6	20.141282177521454	25.30542270361514	42.95066113377409	11.602633985089323
7	60.090378393585596	4.204617386411591	31.87069208046139	3.8343121395414266
8	59.946898297932194	4.1934519623013085	31.667428611619076	4.192221128147419
9	55.663771275847516	5.67370586580391	33.52730693487129	5.135215923477282
10	30.403010268673512	19.098150232100156	39.64551976367984	10.85331973554649
11	23.648456182304123	18.806354620903083	43.276744267829514	14.268444928963287
12	22.230886903924603	16.717013644675763	46.7164861443241	14.335613307075537
13	18.11383457588972	19.63356308904206	47.105078070052045	15.147524265016177
14	14.640068926712619	21.802380784920523	46.77925868617246	16.7782916021944
15	13.667622028414685	20.162821775214518	51.33835630890421	14.831199887466592
16	16.267143761429175	18.92064636376424	48.96970389646926	15.842505978337318
17	16.39163384442256	18.878710085806723	46.2975629483753	18.432093121395415
18	16.359016739344494	19.49140174426783	47.813071458714305	16.336510057673372
19	17.246975664650442	20.593789562526375	45.5603812069208	16.598853565902377
20	19.494478829652554	19.79102194401463	46.07249613166409	14.642003094668729
21	18.090448726965818	19.907159938106624	46.310398790265864	15.691992544661696
22	16.297387114924746	19.72649106766071	45.55897453931636	18.417147278098188
23	16.103091152060767	20.284410606273738	46.402095934730625	17.21040230693487
24	16.86198832465888	18.955725137150093	47.413929525953016	16.76835701223801
25	16.491419327612885	20.13679842453228	44.9750316500211	18.396750597833734
26	16.24947249964833	21.40860177240118	45.43844070896047	16.903485018990015
27	16.988764242509493	20.726719651146432	46.01033900689267	16.2741770994514
28	14.56516387677592	21.36200590800394	46.75086158390772	17.32196863131242
29	16.280858770572515	19.965624560416373	46.48192432128288	17.27159234772823
30	17.554068786045857	21.013767759178506	45.03033127022085	16.40183218455479
31	18.202542551695032	22.62158883105922	42.478548319032214	16.697320298213533
32	18.525284850189898	22.113957659305107	42.18508229005486	17.175675200450133
33	19.184572373048248	23.956164720776478	39.47100506400338	17.388257842171896
34	18.324131382754256	23.91115135743424	38.86200590800394	18.902711351807568
35	19.487972991981994	23.862885075256717	37.46597622731748	19.183165705443802
36	20.882596005064002	26.346268814179208	35.15271135180757	17.61842382894922
37	19.99155999437333	27.04089886059924	36.133862005908	16.833679139119425
38	18.010796173864115	27.61200590800394	35.488465325643546	18.888732592488395
39	20.129149669433115	25.917850611900406	35.49321282880855	18.459786889857927
40	20.55132578421719	25.26023350682234	36.030032353354905	18.15840835560557
41	18.995111830074553	25.686629624419748	35.00545083696722	20.31280770853847
42	20.30296103530736	25.5267091011394	34.35996623997749	19.81036362357575
43	20.328017302011535	25.462881558587707	34.84403572935715	19.365065410043606
44	18.445280630187085	26.686770291180196	34.44427837951892	20.4236706991138
45	18.97911098607399	25.440902377268255	34.30466661977775	21.275320016880013
46	19.243388662259107	25.869056829371218	36.05438528625686	18.833169222112815
47	18.5993986495991	24.982064988043327	37.809027289351526	18.60950907300605
48	18.449500633000422	23.36949641299761	37.97167323111549	20.209329722886483
49	18.457413138275424	23.04121536081024	38.571880714587145	19.929490786327193
50	17.849381066254043	23.562473624982417	37.863535659023775	20.72460964973977
51	17.472306231537488	22.8519306512871	36.6689231959488	23.006839921226614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	334.0
1	708.0
2	1082.0
3	45121.5
4	89161.0
5	61399.0
6	33637.0
7	31867.5
8	30098.0
9	29023.5
10	27949.0
11	26896.5
12	25844.0
13	24348.0
14	22852.0
15	20782.0
16	18712.0
17	16845.0
18	14978.0
19	13839.5
20	12701.0
21	12167.5
22	11634.0
23	10733.5
24	9833.0
25	10303.5
26	11058.0
27	11342.0
28	11773.5
29	12205.0
30	13474.0
31	14743.0
32	16654.0
33	18565.0
34	20237.5
35	21910.0
36	23691.0
37	25472.0
38	27374.0
39	29276.0
40	33674.0
41	38072.0
42	43942.5
43	49813.0
44	54263.0
45	58713.0
46	66649.0
47	74585.0
48	81637.0
49	88689.0
50	88183.5
51	87678.0
52	74776.0
53	61874.0
54	54238.0
55	46602.0
56	42681.0
57	38760.0
58	36802.5
59	34845.0
60	33656.0
61	32467.0
62	30473.0
63	28479.0
64	23772.5
65	19066.0
66	15537.5
67	12009.0
68	10351.5
69	8694.0
70	7159.0
71	5624.0
72	4523.5
73	3423.0
74	2783.0
75	1761.5
76	1380.0
77	1111.0
78	842.0
79	572.0
80	302.0
81	223.5
82	145.0
83	105.0
84	65.0
85	40.0
86	15.0
87	29.5
88	44.0
89	24.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1137440.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.15810906655502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76628158950915	24.36500346922755
2	10.406420359343386	6.693016018163665
3	4.211661203245798	4.063171809760703
4	2.1567092216294776	2.774227614960229
5	1.2249610821059214	1.969621604032374
6	0.7984871724606041	1.5406702548139943
7	0.5604886977083453	1.261697967003345
8	0.4148087301490901	1.0671571508714903
9	0.2988881801927457	0.865051082760822
>10	3.1830847127022923	25.85526173210343
>50	0.8789667959783476	18.42177612270542
>100	0.09283053625490464	4.932483134200327
>500	0.004181555686924683	0.914671224014078
>1k	0.0019513926538981855	1.2660885716701777
>5k	0.0	0.0
>10k+	2.787703791283122E-4	4.010102243712412
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44732	3.9326909551273035	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3618	0.3180827120551414	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3498	0.30753270502180335	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	2143	0.18840554227036152	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	1388	0.12202841468560979	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1267	0.11139049092699395	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1146	0.1007525671683781	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6375017583345054E-4	0.0	0.0	0.1997467998311999	0.0
2	2.6375017583345054E-4	0.0	0.0	1.0892882261921508	0.0
3	2.6375017583345054E-4	0.0	0.0	1.5381910254606836	0.0
4	2.6375017583345054E-4	0.0	0.0	1.9793571529047687	0.0
5	2.6375017583345054E-4	0.0	0.0	3.1074166549444366	0.0
6	2.6375017583345054E-4	0.0	0.0	3.6700836967224646	0.0
7	2.6375017583345054E-4	0.0	0.0	4.243916162610775	0.0
8	2.6375017583345054E-4	0.0	0.0	5.116137994091996	0.0
9	2.6375017583345054E-4	0.0	0.0	5.4265719510479675	0.0
10	2.6375017583345054E-4	0.0	0.0	6.219844563229709	0.0
11	2.6375017583345054E-4	0.0	0.0	7.481361654241103	0.0
12	2.6375017583345054E-4	0.0	0.0	8.40888310592207	0.0
13	2.6375017583345054E-4	0.0	0.0	8.773473765649177	0.0
14	2.6375017583345054E-4	0.0	0.0	8.923283865522578	0.0
15	2.6375017583345054E-4	0.0	0.0	9.25138908425939	0.0
16	2.6375017583345054E-4	0.0	0.0	9.920787030524687	0.0
17	2.6375017583345054E-4	0.0	0.0	10.747467998311999	0.0
18	2.6375017583345054E-4	0.0	0.0	11.655999437332959	0.0
19	2.6375017583345054E-4	0.0	0.0	12.18710437473625	0.0
20	2.6375017583345054E-4	0.0	0.0	12.720231396820932	0.0
21	2.6375017583345054E-4	0.0	0.0	13.380222253481502	0.0
22	3.516669011112674E-4	0.0	0.0	14.078808552539035	0.0
23	3.516669011112674E-4	0.0	0.0	14.740469826979885	0.0
24	4.395836263890843E-4	0.0	0.0	15.283443522295682	0.0
25	4.395836263890843E-4	0.0	0.0	15.774722183148121	0.0
26	4.395836263890843E-4	0.0	0.0	16.232240821493882	0.0
27	5.275003516669011E-4	0.0	0.0	16.654856519904346	0.0
28	6.15417076944718E-4	0.0	0.0	17.124859333239556	0.0
29	6.15417076944718E-4	0.0	0.0	17.592400478266985	0.0
30	6.15417076944718E-4	0.0	0.0	18.109087072724716	0.0
31	6.15417076944718E-4	0.0	0.0	18.61038824025883	0.0
32	6.15417076944718E-4	0.0	0.0	19.07942396961598	0.0
33	6.15417076944718E-4	0.0	0.0	19.540810943873964	0.0
34	6.15417076944718E-4	0.0	0.0	19.989713743142495	0.0
35	6.15417076944718E-4	0.0	0.0	20.47562948375299	0.0
36	6.15417076944718E-4	0.0	0.0	20.94598396398931	0.0
37	6.15417076944718E-4	0.0	0.0	21.398930932620623	0.0
38	7.033338022225348E-4	0.0	0.0	21.844141229427485	0.0
39	7.912505275003516E-4	0.0	0.0	22.293571529047686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGA	30	2.1654814E-6	45.000004	29
TATAGCG	60	0.0	45.000004	1
CAACGCG	20	7.033257E-4	45.0	23
TCCGATA	20	7.033257E-4	45.0	15
ACGTTAC	20	7.033257E-4	45.0	12
ATTCGTT	20	7.033257E-4	45.0	24
ATCCGTG	20	7.033257E-4	45.0	25
ACGATCG	20	7.033257E-4	45.0	23
ACGATAG	25	3.8907812E-5	45.0	1
TGCGATA	20	7.033257E-4	45.0	1
GAACGTA	20	7.033257E-4	45.0	9
CTACCGA	25	3.8907812E-5	45.0	44
CGCACTA	20	7.033257E-4	45.0	15
TATACGA	20	7.033257E-4	45.0	20
GCGTAAG	25	3.8907812E-5	45.0	1
ACGGTAG	25	3.8907812E-5	45.0	1
CGTTTTT	30385	0.0	44.474247	1
CGTTAGG	130	0.0	41.53846	2
CGTTGAT	55	6.184564E-11	40.909092	25
TAACGAA	55	6.184564E-11	40.909092	30
>>END_MODULE