FastQCFastQC Report
Sat 14 Jan 2017
SRR2934965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256247
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386093.0733605731993787No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC85150.6778125639305009No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC65660.5226679148288513No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT42900.341493352819947No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC32770.2608563443335586No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC21990.17504519413777705No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG20790.16549293252043587No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC15730.1252142293673139No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC15590.12409979884529078No Hit
GCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG15060.11988088329763175No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC14720.11717440917271842No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG14200.11303509580520392No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG13580.10809976063624431No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG253.891014E-545.01
AGTCCGT207.0335384E-445.013
GTCGATA207.0335384E-445.018
GTCGACG207.0335384E-445.01
ACTTCGC253.891014E-545.038
CGTCTAA253.891014E-545.029
CATTACG207.0335384E-445.024
TACGAAG207.0335384E-445.01
GTCGTTG207.0335384E-445.029
GTCCGTT207.0335384E-445.014
CGAATTA207.0335384E-445.016
AATTCGT351.2120472E-745.031
CGTCGAA207.0335384E-445.031
CGCGTTA207.0335384E-445.035
CGGTAAG253.891014E-545.015
TACAGCG551.8189894E-1245.01
TATACGA253.891014E-545.041
TACGTAG406.8157533E-945.01
ATTTCGT207.0335384E-445.012
AGGTCCG253.891014E-545.012