##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934965.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1256247
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30020051789178	33.0	31.0	34.0	30.0	34.0
2	32.48589489168929	34.0	31.0	34.0	31.0	34.0
3	32.56401567526132	34.0	31.0	34.0	31.0	34.0
4	36.02237617283862	37.0	35.0	37.0	35.0	37.0
5	35.88573186642436	37.0	35.0	37.0	35.0	37.0
6	35.86683271681445	37.0	35.0	37.0	35.0	37.0
7	36.1027871111334	37.0	35.0	37.0	35.0	37.0
8	36.110213994540885	37.0	36.0	37.0	35.0	37.0
9	37.93124282087838	39.0	39.0	39.0	35.0	39.0
10	37.31024472098242	39.0	37.0	39.0	34.0	39.0
11	37.19608245830637	39.0	37.0	39.0	34.0	39.0
12	37.21912012526199	39.0	37.0	39.0	34.0	39.0
13	37.181449985552206	39.0	37.0	39.0	34.0	39.0
14	38.369045259411564	40.0	38.0	41.0	34.0	41.0
15	38.401110609617376	40.0	38.0	41.0	34.0	41.0
16	38.51880720909184	40.0	38.0	41.0	34.0	41.0
17	38.53567491106446	40.0	38.0	41.0	34.0	41.0
18	38.122089843796644	39.0	38.0	40.0	34.0	41.0
19	37.676876442292	38.0	37.0	40.0	34.0	41.0
20	37.15972575456897	38.0	35.0	40.0	34.0	41.0
21	37.12414596810977	38.0	35.0	40.0	34.0	41.0
22	36.95436725421036	38.0	35.0	40.0	33.0	41.0
23	36.82365410623866	38.0	35.0	40.0	33.0	41.0
24	36.58440099757452	37.0	35.0	40.0	33.0	41.0
25	36.44429320030217	37.0	35.0	40.0	32.0	41.0
26	36.63973326901477	38.0	35.0	40.0	33.0	41.0
27	36.730371495414516	38.0	35.0	40.0	33.0	41.0
28	36.71658678587889	38.0	35.0	40.0	33.0	41.0
29	36.63019135568085	38.0	35.0	40.0	33.0	41.0
30	36.31646881544792	38.0	35.0	40.0	32.0	41.0
31	35.85805976054072	38.0	35.0	40.0	30.0	41.0
32	35.18470810278552	38.0	35.0	40.0	24.0	41.0
33	34.32401828621282	38.0	34.0	40.0	18.0	41.0
34	33.62075770131192	38.0	34.0	40.0	12.0	41.0
35	33.1695510516642	38.0	33.0	40.0	10.0	41.0
36	32.98263080429247	38.0	33.0	40.0	10.0	41.0
37	32.799678725600934	38.0	33.0	40.0	8.0	41.0
38	32.651530312112186	38.0	32.0	40.0	8.0	41.0
39	32.60660045357322	38.0	32.0	40.0	8.0	41.0
40	32.518727606911696	38.0	32.0	40.0	8.0	41.0
41	32.430276848422324	38.0	31.0	40.0	8.0	41.0
42	32.35201914910046	38.0	31.0	40.0	8.0	41.0
43	32.272124032933014	38.0	31.0	40.0	8.0	41.0
44	32.14180411973123	38.0	31.0	40.0	8.0	41.0
45	32.09304460030551	38.0	30.0	40.0	8.0	41.0
46	32.012969583210946	37.0	30.0	40.0	7.0	41.0
47	31.980873188154877	37.0	30.0	40.0	8.0	41.0
48	31.941802846096348	37.0	30.0	40.0	7.0	41.0
49	31.854818359765236	37.0	30.0	40.0	7.0	41.0
50	31.75500040995123	37.0	29.0	40.0	7.0	41.0
51	31.147858661553023	36.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	8.0
11	9.0
12	8.0
13	12.0
14	19.0
15	43.0
16	78.0
17	205.0
18	365.0
19	797.0
20	1383.0
21	2313.0
22	3904.0
23	7234.0
24	14299.0
25	30566.0
26	50987.0
27	57369.0
28	49196.0
29	37786.0
30	31358.0
31	29222.0
32	31189.0
33	38962.0
34	65946.0
35	85928.0
36	91972.0
37	130692.0
38	243791.0
39	250513.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.77647389406701	16.37886498435419	19.45111112703155	32.39354999454725
2	48.22156789230144	18.053137639333666	19.53923074045152	14.186063727913382
3	23.379797125883684	18.29504866479283	44.41817572499676	13.906978484326729
4	21.7201712720508	20.013102518851785	43.74099997850741	14.525726230590003
5	19.104045621601486	21.779634100618747	43.68551725894669	15.43080301883308
6	19.204821981664434	25.161373519697953	42.99544595927393	12.638358539363676
7	60.73351816959563	3.764864712114736	30.818302451667545	4.683314666622089
8	60.376104380746774	3.2159280778381962	30.43231148014682	5.9756560612682055
9	56.77951867745754	4.391970687293183	32.504117422767976	6.324393212481303
10	30.062479751195426	17.42841972955955	39.5682536953322	12.940846823912814
11	21.360369417797614	19.201797098818943	42.37693701955109	17.06089646383235
12	18.53345719432564	16.8637218636144	48.16385631169666	16.438964630363294
13	16.79080626660203	20.048923499916814	48.7381860414393	14.42208419204185
14	15.124016216556138	22.266799443103146	45.80492530529426	16.80425903504645
15	13.581405567535684	21.097443416780298	50.0982688913884	15.22288212429562
16	15.175916838010359	20.42583982290107	46.508608577771724	17.88963476131684
17	15.186265121429146	20.683432477848704	43.86000523782345	20.2702971628987
18	15.720316148018663	20.145003331351237	47.49097908293513	16.643701437694975
19	15.63323136294057	22.098202025557075	44.34589694542554	17.922669666076814
20	16.246168150053293	21.67392240538684	45.91477631389368	16.16513313066618
21	15.880316530109125	21.920131948573808	46.29408070228227	15.905470819034791
22	15.342205792332239	20.019789101983925	44.26303107589511	20.374974029788728
23	14.15923779320468	20.907433012775353	44.84205733426627	20.091271859753697
24	16.54602956265766	18.84923904295891	45.4932827700285	19.111448624354924
25	14.220770278456389	21.411872028351112	43.65837291551741	20.70898477767509
26	13.415196215393948	21.337762398636574	44.91616696398081	20.33087442198867
27	15.26363844052961	20.073122562680748	44.900962947573205	19.76227604921644
28	12.914976115365848	19.459946969027587	46.72671855136768	20.89835836423888
29	15.033190129011253	17.734410510035048	44.92699286048046	22.305406500473236
30	15.65090304693265	19.63841505691158	44.85956981389806	19.851112082257707
31	15.795460606075078	20.15861530415595	43.85976643128302	20.18615765848595
32	16.487840368972027	20.736447529824947	40.87333143880144	21.902380662401583
33	15.863361265738346	20.112127630951555	41.943821557384815	22.080689545925285
34	14.800433354268709	20.6233328318396	42.12810060441935	22.44813320947234
35	15.652654294895829	20.71583056516752	37.279770618357695	26.351744521578958
36	16.698388135454252	21.791017212379412	39.17454131233746	22.336053339828872
37	16.41365113707734	22.647695875094627	40.49657432017748	20.44207866765055
38	17.093055744610734	24.303341619920285	36.82603819153399	21.777564443934992
39	18.857438067513794	21.451752720603512	39.92582668854134	19.76498252334135
40	17.975207104972192	21.297324491123163	38.567256280014995	22.16021212388965
41	15.381768075864061	22.57470067590211	35.94818534890034	26.095345899333488
42	17.034150131303797	21.164468452461975	36.869660186253185	24.931721229981047
43	19.2306926902114	20.75825852718454	36.81123218602711	23.199816596576948
44	17.225672976731488	21.67392240538684	36.49433590687182	24.606068711009858
45	18.186550893255866	20.229461244484565	35.996981485328924	25.58700637693065
46	19.48573807539441	20.532427142114567	37.29776071107035	22.684074071420667
47	14.775517871883476	20.298794743390435	40.87197820173899	24.053709182987106
48	15.93030669923988	19.64764890980834	39.11547649467024	25.306567896281546
49	17.41990229628409	18.2295360705339	41.52774096176946	22.82282067141255
50	17.540977212283888	18.156620473521528	39.76646312389204	24.535939190302543
51	16.15343161018494	18.292979008109075	36.57529132407878	28.9782980576272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	495.0
1	606.0
2	717.0
3	36792.5
4	72868.0
5	50610.0
6	28352.0
7	27612.5
8	26873.0
9	27208.0
10	27543.0
11	27230.0
12	26917.0
13	25681.5
14	24446.0
15	22826.5
16	21207.0
17	19391.0
18	17575.0
19	15926.5
20	14278.0
21	13267.0
22	12256.0
23	11344.0
24	10432.0
25	10125.0
26	9730.5
27	9643.0
28	10588.5
29	11534.0
30	12255.5
31	12977.0
32	14519.0
33	16061.0
34	18047.5
35	20034.0
36	22471.5
37	24909.0
38	28046.0
39	31183.0
40	35069.5
41	38956.0
42	46087.0
43	53218.0
44	64180.5
45	75143.0
46	94179.5
47	113216.0
48	128479.0
49	143742.0
50	140823.5
51	137905.0
52	112754.0
53	87603.0
54	72301.5
55	57000.0
56	47563.0
57	38126.0
58	32586.5
59	27047.0
60	23606.5
61	20166.0
62	18089.0
63	16012.0
64	13393.0
65	10774.0
66	8624.0
67	6474.0
68	5567.5
69	4661.0
70	3605.5
71	2550.0
72	1949.5
73	1349.0
74	1214.5
75	812.5
76	545.0
77	373.0
78	201.0
79	200.5
80	200.0
81	128.0
82	56.0
83	68.0
84	80.0
85	46.5
86	13.0
87	10.5
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1256247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.031898955629494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42603772994657	25.722535344677272
2	11.24257207322437	7.876972977411563
3	4.821715880232291	5.0674159052715515
4	2.621423188774667	3.6733372907639286
5	1.5738600677436765	2.756765343174833
6	1.032607054178303	2.1704511589706725
7	0.7067902952912296	1.7332144345223324
8	0.49122133532780216	1.3766732947242366
9	0.4157005175713771	1.310650067462703
>10	3.0840791291331118	24.085679294535208
>50	0.4922883995941978	11.259066510873138
>100	0.08246459818992839	5.328993223781994
>500	0.005543838475491686	1.3391670715506911
>1k	0.0030029125075579964	1.9544119349865428
>5k	4.619865396243071E-4	1.2203745607623404
>10k+	2.3099326981215355E-4	3.1242915865309464
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38609	3.0733605731993787	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	8515	0.6778125639305009	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	6566	0.5226679148288513	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	4290	0.341493352819947	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	3277	0.2608563443335586	No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	2199	0.17504519413777705	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	2079	0.16549293252043587	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	1573	0.1252142293673139	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	1559	0.12409979884529078	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	1506	0.11988088329763175	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	1472	0.11717440917271842	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	1420	0.11303509580520392	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	1358	0.10809976063624431	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.96021801445098E-5	0.0	0.0	0.3623491240178086	0.0
2	7.96021801445098E-5	0.0	0.0	2.1053184604619952	0.0
3	7.96021801445098E-5	0.0	0.0	2.983250905275794	0.0
4	7.96021801445098E-5	0.0	0.0	3.9694423150861256	0.0
5	7.96021801445098E-5	0.0	0.0	6.349627103587113	0.0
6	7.96021801445098E-5	0.0	0.0	7.516913473226205	0.0
7	7.96021801445098E-5	0.0	0.0	8.758389074759979	0.0
8	7.96021801445098E-5	0.0	0.0	10.634214449865352	0.0
9	1.592043602890196E-4	0.0	0.0	11.246912430437645	0.0
10	1.592043602890196E-4	0.0	0.0	12.854956071536888	0.0
11	1.592043602890196E-4	0.0	0.0	15.141210287467354	0.0
12	1.592043602890196E-4	0.0	0.0	16.992677395448506	0.0
13	1.592043602890196E-4	0.0	0.0	17.6488381663797	0.0
14	1.592043602890196E-4	0.0	0.0	17.88724669591251	0.0
15	1.592043602890196E-4	0.0	0.0	18.36087966777234	0.0
16	1.592043602890196E-4	0.0	0.0	19.431130979815276	0.0
17	1.592043602890196E-4	0.0	0.0	20.77569140463619	0.0
18	1.592043602890196E-4	0.0	0.0	22.21633166089153	0.0
19	1.592043602890196E-4	0.0	0.0	23.16686129399712	0.0
20	1.592043602890196E-4	0.0	0.0	24.020753880407277	0.0
21	1.592043602890196E-4	0.0	0.0	25.058049889870382	0.0
22	1.592043602890196E-4	0.0	0.0	26.110231507020515	0.0
23	2.388065404335294E-4	0.0	0.0	27.194174394048304	0.0
24	2.388065404335294E-4	0.0	0.0	28.008982310007507	0.0
25	2.388065404335294E-4	0.0	0.0	28.730018857756477	0.0
26	2.388065404335294E-4	0.0	0.0	29.388965704992728	0.0
27	2.388065404335294E-4	0.0	0.0	30.003176126987768	0.0
28	2.388065404335294E-4	0.0	0.0	30.6565508216139	0.0
29	2.388065404335294E-4	0.0	0.0	31.341129570856687	0.0
30	2.388065404335294E-4	0.0	0.0	32.09185773179956	0.0
31	2.388065404335294E-4	0.0	0.0	32.794665380295434	0.0
32	3.184087205780392E-4	0.0	0.0	33.423522603437064	0.0
33	3.98010900722549E-4	0.0	0.0	34.05397187018158	0.0
34	3.98010900722549E-4	0.0	0.0	34.664440989709824	0.0
35	3.98010900722549E-4	0.0	0.0	35.29138776052798	0.0
36	3.98010900722549E-4	0.0	0.0	35.87009561017857	0.0
37	3.98010900722549E-4	0.0	0.0	36.43821636986994	0.0
38	3.98010900722549E-4	0.0	0.0	37.03594914057506	0.0
39	3.98010900722549E-4	0.0	0.0	37.585841001013335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	25	3.891014E-5	45.0	1
AGTCCGT	20	7.0335384E-4	45.0	13
GTCGATA	20	7.0335384E-4	45.0	18
GTCGACG	20	7.0335384E-4	45.0	1
ACTTCGC	25	3.891014E-5	45.0	38
CGTCTAA	25	3.891014E-5	45.0	29
CATTACG	20	7.0335384E-4	45.0	24
TACGAAG	20	7.0335384E-4	45.0	1
GTCGTTG	20	7.0335384E-4	45.0	29
GTCCGTT	20	7.0335384E-4	45.0	14
CGAATTA	20	7.0335384E-4	45.0	16
AATTCGT	35	1.2120472E-7	45.0	31
CGTCGAA	20	7.0335384E-4	45.0	31
CGCGTTA	20	7.0335384E-4	45.0	35
CGGTAAG	25	3.891014E-5	45.0	15
TACAGCG	55	1.8189894E-12	45.0	1
TATACGA	25	3.891014E-5	45.0	41
TACGTAG	40	6.8157533E-9	45.0	1
ATTTCGT	20	7.0335384E-4	45.0	12
AGGTCCG	25	3.891014E-5	45.0	12
>>END_MODULE