##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934964.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1005244
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.513489262308454	34.0	31.0	34.0	31.0	34.0
2	32.68529630617044	34.0	31.0	34.0	31.0	34.0
3	32.74981099116234	34.0	31.0	34.0	31.0	34.0
4	36.1635642689735	37.0	37.0	37.0	35.0	37.0
5	36.04717859544549	37.0	35.0	37.0	35.0	37.0
6	36.032343391256255	37.0	35.0	37.0	35.0	37.0
7	36.22895635288547	37.0	37.0	37.0	35.0	37.0
8	36.24362940738766	37.0	37.0	37.0	35.0	37.0
9	38.05837388733482	39.0	39.0	39.0	35.0	39.0
10	37.48666293954503	39.0	37.0	39.0	34.0	39.0
11	37.40817154840019	39.0	37.0	39.0	34.0	39.0
12	37.39303790920413	39.0	37.0	39.0	35.0	39.0
13	37.357568908643074	39.0	37.0	39.0	34.0	39.0
14	38.601846914778896	40.0	38.0	41.0	34.0	41.0
15	38.66508927185838	40.0	38.0	41.0	34.0	41.0
16	38.76605779293386	40.0	38.0	41.0	35.0	41.0
17	38.78311335357386	40.0	38.0	41.0	35.0	41.0
18	38.28194945704725	39.0	38.0	41.0	35.0	41.0
19	37.79750190003621	38.0	37.0	40.0	35.0	41.0
20	37.23712849815567	38.0	35.0	40.0	34.0	41.0
21	37.188913338453155	38.0	35.0	40.0	34.0	41.0
22	37.01875365582883	38.0	35.0	40.0	34.0	41.0
23	36.90617700777125	38.0	35.0	40.0	33.0	41.0
24	36.693752959480484	37.0	35.0	40.0	33.0	41.0
25	36.53736008372097	37.0	35.0	40.0	33.0	41.0
26	36.6995873638639	37.0	35.0	40.0	33.0	41.0
27	36.77022295084576	38.0	35.0	40.0	33.0	41.0
28	36.73234060586285	38.0	35.0	40.0	33.0	41.0
29	36.59659744300886	38.0	35.0	40.0	33.0	41.0
30	36.2714226595732	38.0	35.0	40.0	33.0	41.0
31	35.71877375045263	38.0	35.0	40.0	30.0	41.0
32	34.94864430924233	38.0	35.0	40.0	23.0	41.0
33	33.99649139910311	38.0	34.0	40.0	16.0	41.0
34	33.22744726653429	38.0	33.0	40.0	10.0	41.0
35	32.76395581570246	38.0	33.0	40.0	10.0	41.0
36	32.58560906605759	38.0	33.0	40.0	8.0	41.0
37	32.389649677093324	38.0	31.0	40.0	8.0	41.0
38	32.26991556278874	38.0	31.0	40.0	8.0	41.0
39	32.19769031200385	37.0	31.0	40.0	8.0	41.0
40	32.06640278380174	37.0	31.0	40.0	7.0	41.0
41	31.9348964032613	37.0	30.0	40.0	7.0	41.0
42	31.7999460827421	37.0	30.0	40.0	7.0	41.0
43	31.697945971326366	37.0	29.0	40.0	7.0	41.0
44	31.591433522607446	37.0	29.0	40.0	7.0	41.0
45	31.569874577714465	37.0	29.0	40.0	7.0	41.0
46	31.52190711906761	37.0	28.0	40.0	7.0	41.0
47	31.501151959126343	37.0	28.0	40.0	7.0	41.0
48	31.493630402171014	37.0	28.0	40.0	7.0	41.0
49	31.417626964199737	36.0	27.0	40.0	7.0	41.0
50	31.336223842171652	36.0	27.0	40.0	7.0	41.0
51	30.78106907377711	35.0	26.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	5.0
11	9.0
12	7.0
13	16.0
14	15.0
15	37.0
16	71.0
17	151.0
18	329.0
19	668.0
20	1043.0
21	1764.0
22	3029.0
23	5367.0
24	10979.0
25	24336.0
26	42140.0
27	50849.0
28	44674.0
29	34283.0
30	26887.0
31	23583.0
32	24411.0
33	30119.0
34	52283.0
35	68643.0
36	69611.0
37	89731.0
38	173418.0
39	226643.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.04568443084465	15.688031960399664	19.167983096641215	36.09830051211447
2	50.77811954112633	17.976033679385303	18.568228211260152	12.67761856822821
3	22.374070374953742	17.74564185411701	46.40714095284329	13.473146818085958
4	20.840412874884105	18.39861764904839	46.885830703789324	13.875138772278174
5	18.500682421382272	21.610673627497405	45.323921356407006	14.564722594713322
6	19.315907381690415	24.4662987294627	44.045127352165245	12.172666536681641
7	59.825873121351634	3.6338441214272357	32.58671526514956	3.953567492071577
8	60.491681621576454	3.1749505592672027	32.05798791139266	4.275379907763687
9	56.83744444134956	4.2897047880912496	33.770308502214384	5.1025422683448
10	28.416185523116777	18.320626633931663	40.506285041243714	12.756902801707845
11	21.13417239993474	19.592457154680854	44.73033412783364	14.543036317550765
12	19.84702221550191	16.68012940141896	48.3804926963006	15.092355686778532
13	16.204722435547986	19.435082427748885	48.98472410678402	15.375471029919105
14	13.731591533995728	21.965015458933355	48.068130722491254	16.23526228457966
15	12.915172833660286	20.08666552598175	52.12296716021185	14.875194480146114
16	14.996955962930391	19.60359872826896	49.140905093688694	16.258540215111953
17	14.824659485657213	19.838864991982046	46.52084469044331	18.815630831917424
18	15.334684912319796	19.12739593571312	49.53623199939517	16.001687152571915
19	15.9842784438405	20.407881071660213	47.2268424382538	16.380998046245487
20	16.5906983777073	21.017086398924043	47.213412862946704	15.178802360421948
21	16.194078253637922	20.54018725801895	47.80222513141088	15.463509356932247
22	14.928614346367647	19.4673134084859	47.333781649032474	18.270290596113977
23	14.412520741232973	19.791513304232605	47.94199219294022	17.8539737615942
24	15.710713020918304	18.25566728077959	48.81143284615476	17.22218685214734
25	14.10334207416309	21.081846795404896	46.665088277075014	18.149722853356995
26	13.696077768183645	21.220818030249372	46.68776933759366	18.39533486397332
27	14.462558344043835	20.756552637966504	47.84758725244816	16.9333017655415
28	12.012804851359471	19.935856369199918	48.478379378538946	19.57295940090167
29	13.342034371754519	19.429511640954832	47.64087127105459	19.587582716236057
30	14.405258822733586	20.544066913107663	46.87150582346177	18.179168440696987
31	14.918169121128798	22.335373302402203	43.45969734711175	19.28676022935725
32	16.433323650775332	23.345575800502168	41.42536538392669	18.79573516479581
33	15.801337784657257	23.533390898130204	40.51941618154398	20.14585513566855
34	15.006306926477553	24.03456275292367	39.1922757061967	21.766854614402074
35	16.15707231279172	25.11907556772286	37.103927006776466	21.619925112708955
36	16.88376155440868	26.204782122549354	36.289199438146355	20.622256884895606
37	16.611190914842567	27.146841960757783	37.39808444516953	18.843882679230116
38	16.652474424119916	27.804891150805176	34.411048461865974	21.13158596320893
39	17.17582994775398	27.08218104261254	35.555049321358794	20.186939688274688
40	18.550521067521917	25.214873204913435	35.90302453931582	20.331581188248823
41	17.381849580798296	26.097345520092635	34.1537974859835	22.36700741312557
42	17.778967096545713	25.83551854077219	34.7527565446797	21.632757818002396
43	18.0179140586763	25.816816613677872	34.89859178468113	21.266677542964693
44	16.4082551102021	25.510423340005012	34.76718090334287	23.314140646450017
45	16.895400519674826	25.113305824257594	34.22114431919017	23.770149336877413
46	18.235871091993587	24.584677948836305	35.4848176164195	21.694633342750617
47	15.51901826820155	25.007361396834998	37.93516797911751	21.538452355845944
48	16.39850623331251	23.741400097886682	37.07209393938189	22.787999729418928
49	17.019648960849306	22.884493714958758	38.36600865063606	21.729848673555875
50	16.200643823788056	22.62734221741189	38.02977187628078	23.142242082519267
51	15.773185415680175	23.104340836652593	35.72306823020083	25.399405517466406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338.0
1	543.5
2	749.0
3	37446.0
4	74143.0
5	50687.5
6	27232.0
7	26596.0
8	25960.0
9	25782.5
10	25605.0
11	24816.5
12	24028.0
13	22554.0
14	21080.0
15	19421.0
16	17762.0
17	16144.5
18	14527.0
19	13063.0
20	11599.0
21	10522.5
22	9446.0
23	9026.0
24	8606.0
25	8681.5
26	10019.5
27	11282.0
28	11943.0
29	12604.0
30	13218.5
31	13833.0
32	15245.0
33	16657.0
34	18735.5
35	20814.0
36	22223.0
37	23632.0
38	25631.0
39	27630.0
40	31526.5
41	35423.0
42	40957.5
43	46492.0
44	51414.5
45	56337.0
46	66004.0
47	75671.0
48	83070.5
49	90470.0
50	88294.0
51	86118.0
52	72510.5
53	58903.0
54	50718.0
55	42533.0
56	36789.5
57	31046.0
58	27877.5
59	24709.0
60	22420.0
61	20131.0
62	17207.0
63	14283.0
64	12409.0
65	10535.0
66	8601.0
67	6667.0
68	5307.5
69	3948.0
70	3185.5
71	2423.0
72	1940.5
73	1458.0
74	1189.5
75	725.0
76	529.0
77	344.0
78	159.0
79	122.0
80	85.0
81	80.0
82	75.0
83	43.5
84	12.0
85	19.5
86	27.0
87	14.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1005244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.25315450129015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77736305567065	25.95613724446243
2	10.49367570371722	7.188829903317212
3	4.181812813816857	4.297208412214312
4	2.1759540891602107	2.981331664148751
5	1.303395130159153	2.232269738478533
6	0.8386621672746042	1.7236094874026313
7	0.5624028401078625	1.3484849961925294
8	0.4034402069058873	1.1055279791343857
9	0.3003259806797646	0.9258400995277912
>10	3.2670547731072106	28.054758191255825
>50	0.6199041293164511	13.55662220057488
>100	0.06831947248817019	3.9443303109305305
>500	0.005323595258780322	1.235522087373733
>1k	0.0020702870450812366	1.5524057450733917
>5k	0.0	0.0
>10k+	2.9575529215446233E-4	3.8971219399130974
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38469	3.826832092506894	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	4074	0.40527473926728236	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3621	0.3602110532368261	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	2532	0.2518791457596365	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1696	0.16871525719128888	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	1248	0.12414896283887294	No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1077	0.10713816744989275	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	1076	0.1070386891142847	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.947833560807128E-5	0.0	0.0	0.2675967227857117	0.0
2	9.947833560807128E-5	0.0	0.0	1.3753874681171934	0.0
3	9.947833560807128E-5	0.0	0.0	1.931471364166312	0.0
4	9.947833560807128E-5	0.0	0.0	2.5573890518122964	0.0
5	9.947833560807128E-5	0.0	0.0	4.0813971533279485	0.0
6	9.947833560807128E-5	0.0	0.0	4.813856138410177	0.0
7	9.947833560807128E-5	0.0	0.0	5.594164202919888	0.0
8	9.947833560807128E-5	0.0	0.0	6.771092391498979	0.0
9	9.947833560807128E-5	0.0	0.0	7.139162233248843	0.0
10	9.947833560807128E-5	0.0	0.0	8.148568904663943	0.0
11	9.947833560807128E-5	0.0	0.0	9.641738722141092	0.0
12	9.947833560807128E-5	0.0	0.0	10.79409576182499	0.0
13	9.947833560807128E-5	0.0	0.0	11.20882094297504	0.0
14	9.947833560807128E-5	0.0	0.0	11.358038446387146	0.0
15	9.947833560807128E-5	0.0	0.0	11.676369120332973	0.0
16	9.947833560807128E-5	0.0	0.0	12.397984966833922	0.0
17	9.947833560807128E-5	0.0	0.0	13.286028068807175	0.0
18	9.947833560807128E-5	0.0	0.0	14.309660142214229	0.0
19	9.947833560807128E-5	0.0	0.0	14.91458790104691	0.0
20	9.947833560807128E-5	0.0	0.0	15.481713892348525	0.0
21	9.947833560807128E-5	0.0	0.0	16.257246996749046	0.0
22	9.947833560807128E-5	0.0	0.0	17.046607589799095	0.0
23	1.9895667121614256E-4	0.0	0.0	17.79468467357179	0.0
24	1.9895667121614256E-4	0.0	0.0	18.387873988802717	0.0
25	1.9895667121614256E-4	0.0	0.0	18.926847611127247	0.0
26	1.9895667121614256E-4	0.0	0.0	19.406532145429367	0.0
27	1.9895667121614256E-4	0.0	0.0	19.890693204833852	0.0
28	1.9895667121614256E-4	0.0	0.0	20.364607995670703	0.0
29	1.9895667121614256E-4	0.0	0.0	20.903482139659626	0.0
30	1.9895667121614256E-4	0.0	0.0	21.494781366514	0.0
31	1.9895667121614256E-4	0.0	0.0	22.01525201841543	0.0
32	1.9895667121614256E-4	0.0	0.0	22.508565084695856	0.0
33	2.984350068242138E-4	0.0	0.0	22.978898655450816	0.0
34	2.984350068242138E-4	0.0	0.0	23.455598839684694	0.0
35	3.979133424322851E-4	0.0	0.0	23.981142886702134	0.0
36	3.979133424322851E-4	0.0	0.0	24.435559923759804	0.0
37	3.979133424322851E-4	0.0	0.0	24.890573830831123	0.0
38	3.979133424322851E-4	0.0	0.0	25.360211053236824	0.0
39	3.979133424322851E-4	0.0	0.0	25.85471785954455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	45	3.8380676E-10	45.000004	1
CTATGCG	25	3.8904553E-5	45.0	1
TAACGCG	40	6.8139343E-9	45.0	1
ATCGTTA	20	7.032865E-4	45.0	28
CCCCGTA	20	7.032865E-4	45.0	32
CCGTAGG	25	3.8904553E-5	45.0	2
CGTAGCA	20	7.032865E-4	45.0	24
ACAATCG	20	7.032865E-4	45.0	12
CGGGCCG	25	3.8904553E-5	45.0	6
TATTACG	35	1.2117198E-7	45.0	1
TATAGCG	20	7.032865E-4	45.0	1
CGTCGAC	20	7.032865E-4	45.0	26
CCGCGTG	20	7.032865E-4	45.0	39
ACCGTAG	20	7.032865E-4	45.0	22
GACGCGA	20	7.032865E-4	45.0	14
ATCGGCA	20	7.032865E-4	45.0	27
CAATCGT	20	7.032865E-4	45.0	13
ATACCGG	40	6.8139343E-9	45.0	2
GCGTACG	70	0.0	45.0	1
CACCCGC	25	3.8904553E-5	45.0	28
>>END_MODULE