##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934963.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498036
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.280451613939555	33.0	31.0	34.0	30.0	34.0
2	32.45028672626075	34.0	31.0	34.0	30.0	34.0
3	32.548127043025005	34.0	31.0	34.0	31.0	34.0
4	36.045095133685116	37.0	35.0	37.0	35.0	37.0
5	35.89778048173224	37.0	35.0	37.0	35.0	37.0
6	35.807736790111555	37.0	35.0	37.0	35.0	37.0
7	36.042035113927504	37.0	35.0	37.0	35.0	37.0
8	35.82826743448265	37.0	35.0	37.0	35.0	37.0
9	37.76894642154382	39.0	38.0	39.0	35.0	39.0
10	37.35439405986716	39.0	37.0	39.0	34.0	39.0
11	37.25963785750427	39.0	37.0	39.0	34.0	39.0
12	37.15207535198259	39.0	37.0	39.0	34.0	39.0
13	36.90509722188757	39.0	37.0	39.0	33.0	39.0
14	38.04440843633794	40.0	37.0	41.0	33.0	41.0
15	38.04667935651238	40.0	37.0	41.0	33.0	41.0
16	38.292701732404886	40.0	37.0	41.0	34.0	41.0
17	38.211571051088676	40.0	37.0	41.0	34.0	41.0
18	37.880175328691095	39.0	37.0	40.0	34.0	41.0
19	37.49686167265017	38.0	37.0	40.0	34.0	41.0
20	36.82694423696279	38.0	35.0	40.0	33.0	41.0
21	36.86586311029725	38.0	35.0	40.0	33.0	41.0
22	36.73439269450401	38.0	35.0	40.0	33.0	41.0
23	36.43738806030086	37.0	35.0	40.0	32.0	41.0
24	36.26896449252664	37.0	35.0	40.0	32.0	41.0
25	36.15714526660723	37.0	35.0	40.0	32.0	41.0
26	36.36759792464802	37.0	35.0	40.0	32.0	41.0
27	36.35375354392052	37.0	35.0	40.0	32.0	41.0
28	36.32693419752789	37.0	35.0	40.0	32.0	41.0
29	36.255808817033305	37.0	35.0	40.0	32.0	41.0
30	35.92708559220618	37.0	35.0	40.0	31.0	41.0
31	35.488398429029225	37.0	35.0	40.0	29.0	41.0
32	34.69622276301312	37.0	35.0	40.0	23.0	41.0
33	33.824932334208775	37.0	33.0	40.0	17.0	41.0
34	33.162709121428975	37.0	33.0	40.0	12.0	41.0
35	32.795048952284574	37.0	32.0	40.0	10.0	41.0
36	32.64440522371877	37.0	32.0	40.0	10.0	41.0
37	32.424198250728864	37.0	31.0	40.0	10.0	41.0
38	32.248066404838205	36.0	31.0	40.0	10.0	41.0
39	32.10225766812038	36.0	31.0	40.0	9.0	41.0
40	31.807533993526572	36.0	30.0	40.0	8.0	41.0
41	31.593541430739947	36.0	29.0	40.0	8.0	41.0
42	31.469933900360616	35.0	30.0	40.0	8.0	41.0
43	31.241518685396237	35.0	29.0	39.0	8.0	41.0
44	31.09326233444972	35.0	29.0	39.0	8.0	41.0
45	30.896188227357058	35.0	29.0	39.0	8.0	41.0
46	30.744648981198146	35.0	28.0	39.0	8.0	41.0
47	30.489789894706405	35.0	28.0	38.0	8.0	41.0
48	30.488518902248032	35.0	28.0	38.0	8.0	41.0
49	30.424517504758693	35.0	28.0	38.0	7.0	41.0
50	30.25736693732983	35.0	27.0	37.0	7.0	41.0
51	29.694869447188555	34.0	26.0	36.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	7.0
12	3.0
13	5.0
14	5.0
15	23.0
16	39.0
17	97.0
18	168.0
19	428.0
20	853.0
21	1664.0
22	2578.0
23	3795.0
24	6357.0
25	11550.0
26	18445.0
27	22002.0
28	19707.0
29	16346.0
30	14401.0
31	14269.0
32	15523.0
33	19892.0
34	33679.0
35	46451.0
36	46037.0
37	59787.0
38	71813.0
39	72057.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.53228280686537	13.30124729939201	15.919331132689205	31.247138761053417
2	43.96389016055065	27.607040454906873	15.781389297159244	12.647680087383243
3	20.3726236657591	27.60483177922881	39.61721642612181	12.40532812889028
4	18.938590784601917	17.070854315752275	51.287256342914965	12.703298556730838
5	25.760186010649832	19.62428418829161	39.17588286790513	15.439646933153425
6	16.731119838726517	32.248070420612166	39.90635215125011	11.114457589411208
7	54.282421351066986	3.5997397778473847	37.88922889108418	4.228609980001446
8	52.32754258728285	11.990498678810367	30.06469411849746	5.6172646154093275
9	49.57332401673774	4.080026343477178	30.537350713603033	15.809298926182045
10	34.477226545872185	14.344143796833963	37.775783276710925	13.40284638058293
11	27.387979985382582	16.69778891485756	39.73226835007911	16.181962749680746
12	21.971303279281017	14.141146423150133	44.6692608566449	19.21828944092395
13	18.111341348818158	20.091117911155017	48.962123220008195	12.835417520018632
14	15.093487217791484	26.05454224192629	41.93833377506847	16.91363676521376
15	10.855239380285763	17.804737006963354	55.33355018512718	16.006473427623703
16	12.000939691106668	19.098017010818495	45.09513368511513	23.805909612959706
17	12.592061618035643	19.972853368029618	46.19204234232063	21.243042671614102
18	13.579540434828004	18.920519801781396	47.25321061128111	20.246729152109484
19	14.427069529110344	20.535061722445764	43.34445702720286	21.693411721241034
20	17.393923330843553	19.720863552032384	47.74654844228128	15.138664674842783
21	15.998040302307464	25.058027933723665	43.54745440088668	15.396477363082187
22	13.976901268181416	17.897702174140022	45.497112658522674	22.628283899155885
23	14.395344914825433	23.306548121019365	43.97714221461902	18.320964749536177
24	16.7166630524701	18.354898039499155	43.06716783525689	21.861271072773857
25	12.535037627802007	26.740637223011994	41.86424274550434	18.86008240368166
26	13.959633440152922	20.086298982402877	45.695491892152376	20.258575685291827
27	18.86449975503779	21.2673381040728	41.38877510862669	18.47938703226273
28	12.539454979158132	19.82567525239139	47.54274791380543	20.092121854645047
29	20.82560296846011	16.233967022464242	42.85633970235083	20.084090306724814
30	18.076002537969142	24.109301335646418	41.69417471829346	16.120521408090983
31	19.582720927804413	20.983422885092644	36.04900047386133	23.384855713241613
32	24.885148864740703	25.199182388421722	34.11520452336779	15.80046422346979
33	18.112947658402202	25.63850805965834	33.03496132809676	23.213582953842693
34	21.225172477491586	25.11204009348722	33.49155482736188	20.171232601659316
35	20.92338706438892	26.136062453316626	31.743287633825666	21.197262848468785
36	22.183336144375104	27.390590238456657	30.390373386662812	20.035700230505427
37	22.858387747070495	24.90763719891735	34.37863929515135	17.855335758860804
38	17.21963874097455	29.55810423342891	29.145282670329053	24.07697435526749
39	22.483716036591733	29.173192299351854	31.603137122617643	16.73995454143877
40	18.698648290485025	29.580190990209545	32.807668521954234	18.913492197351196
41	16.543984772185144	32.74923901083456	28.877631335887365	21.829144881092933
42	19.327518492639086	25.83046205495185	34.05115292870395	20.790866523705116
43	21.998008176115782	27.00186331911749	29.308122304411725	21.692006200354992
44	20.81194933699572	23.35473740854075	33.389955746170955	22.443357508292575
45	18.124794191584545	23.163184990643245	31.811154213751617	26.90086660402059
46	24.314908962404324	26.764531078074675	30.704808487739843	18.215751471781154
47	15.695652523110779	25.02409464376069	40.001124416708834	19.279128416419695
48	19.266880305841344	25.229702270518594	32.231204169979684	23.27221325366038
49	17.37083263057289	21.328177079568544	39.37767550940093	21.923314780457638
50	19.327116915243074	22.567645712358143	35.27134584648499	22.83389152591379
51	18.218562513553238	21.932952637961915	32.20992056799107	27.638564280493778
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	414.5
2	484.0
3	15376.5
4	30269.0
5	20458.0
6	10647.0
7	10655.0
8	10663.0
9	10803.0
10	10943.0
11	10636.5
12	10330.0
13	9674.5
14	9019.0
15	8172.5
16	7326.0
17	6723.5
18	6121.0
19	5514.5
20	4908.0
21	4464.0
22	4020.0
23	3793.0
24	3566.0
25	3476.5
26	4056.0
27	4725.0
28	4560.5
29	4396.0
30	4393.0
31	4390.0
32	5012.0
33	5634.0
34	6507.0
35	7380.0
36	7528.0
37	7676.0
38	8612.5
39	9549.0
40	11793.0
41	14037.0
42	16724.0
43	19411.0
44	23487.0
45	27563.0
46	51626.0
47	75689.0
48	61853.5
49	48018.0
50	47311.5
51	46605.0
52	38337.5
53	30070.0
54	25625.5
55	21181.0
56	18621.0
57	16061.0
58	14079.0
59	12097.0
60	10847.5
61	9598.0
62	8295.5
63	6993.0
64	6332.5
65	5672.0
66	4515.0
67	3358.0
68	2763.0
69	2168.0
70	1720.5
71	1273.0
72	1290.0
73	1307.0
74	918.0
75	483.5
76	438.0
77	269.0
78	100.0
79	64.0
80	28.0
81	37.5
82	47.0
83	26.5
84	6.0
85	5.5
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.807614060700274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.63027323561622	25.906867029157226
2	10.260565766742245	6.937704949729101
3	4.101638103863028	4.159997940961912
4	2.0807680163779447	2.813832081902177
5	1.219494203097585	2.06140946837922
6	0.7634581206159299	1.5486418495974552
7	0.5234839675209082	1.2388420758637715
8	0.36543589808855015	0.9883612645202482
9	0.2937283377671106	0.8937228853729339
>10	3.304595430283825	27.65413816063349
>50	0.3999407953795871	8.449023494373925
>100	0.04577635609766359	2.8508225422065685
>500	0.0036139228498155464	0.7827544180172893
>1k	0.004818563799754062	3.632762439497513
>5k	6.023204749692578E-4	1.7897056659609667
>10k+	0.0018069614249077732	8.291413733826218
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16323	3.2774739175481296	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	12463	2.50242954324587	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	11932	2.3958107446048076	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	8789	1.764731866772683	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	4352	0.8738324137210965	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT	3243	0.6511577476327013	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT	2162	0.4341051650884675	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	2115	0.4246680962821965	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA	1825	0.36643937386052416	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC	1431	0.2873286268462521	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	1389	0.2788955015300099	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT	1323	0.26564344746162927	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	762	0.15300098788039418	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC	674	0.13533158245588672	No Hit
CGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	656	0.13171738589178292	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGCGAAAA	611	0.12268189448152343	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATG	574	0.11525271265531006	No Hit
ACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	567	0.11384719176926969	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30258856789469035	0.0
2	0.0	0.0	0.0	1.9976467564593725	0.0
3	0.0	0.0	0.0	2.585756852918263	0.0
4	0.0	0.0	0.0	3.443726959496904	0.0
5	0.0	0.0	0.0	6.812961312033668	0.0
6	0.0	0.0	0.0	7.584592278469829	0.0
7	0.0	0.0	0.0	8.813218321567115	0.0
8	0.0	0.0	0.0	10.258495369812623	0.0
9	0.0	0.0	0.0	10.653647527487973	0.0
10	0.0	0.0	0.0	14.281296934358158	0.0
11	0.0	0.0	0.0	15.824960444626493	0.0
12	0.0	0.0	0.0	19.568264141548	0.0
13	0.0	0.0	0.0	20.154968717120852	0.0
14	0.0	0.0	0.0	20.42382478375057	0.0
15	0.0	0.0	0.0	21.146061730477314	0.0
16	0.0	0.0	0.0	22.016480736332312	0.0
17	0.0	0.0	0.0	22.952356857737193	0.0
18	0.0	0.0	0.0	24.009509352737552	0.0
19	0.0	0.0	0.0	25.313431157587	0.0
20	0.0	0.0	0.0	25.995911942108602	0.0
21	0.0	0.0	0.0	26.73019621071569	0.0
22	0.0	0.0	0.0	27.42793693628573	0.0
23	0.0	0.0	0.0	28.162020416194814	0.0
24	0.0	0.0	0.0	28.703949112112376	0.0
25	0.0	0.0	0.0	29.227204459115406	0.0
26	0.0	0.0	0.0	29.693034238488785	0.0
27	0.0	0.0	0.0	30.149226160357887	0.0
28	0.0	0.0	0.0	30.599795998682826	0.0
29	0.0	0.0	0.0	31.071649438996378	0.0
30	0.0	0.0	0.0	31.610967881839866	0.0
31	2.0078869800576666E-4	0.0	0.0	32.103301769350004	0.0
32	2.0078869800576666E-4	0.0	0.0	32.52636355604816	0.0
33	2.0078869800576666E-4	0.0	0.0	32.954244271498446	0.0
34	2.0078869800576666E-4	0.0	0.0	33.37770763559261	0.0
35	2.0078869800576666E-4	0.0	0.0	33.907589009629824	0.0
36	2.0078869800576666E-4	0.0	0.0	34.353540707900635	0.0
37	2.0078869800576666E-4	0.0	0.0	34.79206322434523	0.0
38	2.0078869800576666E-4	0.0	0.0	35.21813684151346	0.0
39	2.0078869800576666E-4	0.0	0.0	35.60646218345662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.02943E-4	45.000004	21
GTCATCA	30	2.1630149E-6	45.000004	10
CGGGTGC	20	7.02943E-4	45.000004	6
CGGGTCG	20	7.02943E-4	45.000004	8
TGTCAAG	20	7.02943E-4	45.000004	1
CGTGGAA	20	7.02943E-4	45.000004	6
CCGGGAC	40	6.8012014E-9	45.000004	5
CTGTACG	20	7.02943E-4	45.000004	35
ATCTACC	20	7.02943E-4	45.000004	15
GTACACG	40	6.8012014E-9	45.000004	1
ACGCACG	20	7.02943E-4	45.000004	43
GACGTAG	30	2.1630149E-6	45.000004	1
ATCAGGC	20	7.02943E-4	45.000004	12
TAGAACG	60	0.0	45.000004	1
GACCGAG	20	7.02943E-4	45.000004	30
ACCGACG	20	7.02943E-4	45.000004	17
TAAGTTG	20	7.02943E-4	45.000004	11
AAGCGAT	20	7.02943E-4	45.000004	36
CGACACT	20	7.02943E-4	45.000004	12
CCTCGGT	20	7.02943E-4	45.000004	34
>>END_MODULE