FastQCFastQC Report
Sat 14 Jan 2017
SRR2934962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560571
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176213.1434019954653376No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC24440.43598402343324927TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC23110.4122582152840586TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT13810.24635594777467976TruSeq Adapter, Index 14 (95% over 23bp)
GCCGAAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCTT9800.1748217442571949No Hit
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC9310.16608065704433517TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG8870.158231517506257TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC7970.1421764593601881No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG6540.11666675586143416No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC6240.1113150698127445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG302.1635042E-645.0000041
CGAACTA302.1635042E-645.00000429
CACGACC351.2104465E-745.00000427
TACGCCG302.1635042E-645.0000043
CCGAGGT351.2104465E-745.0000047
TTACGAG302.1635042E-645.0000041
CGAGACC302.1635042E-645.00000436
AGTAGCG302.1635042E-645.0000041
CCTATCC302.1635042E-645.00000422
TACAGCG302.1635042E-645.0000041
ACGAGAC351.2104465E-745.00000435
TACACGC650.045.00000435
TCGCCAA207.030192E-445.019
CTTAACG253.8882397E-545.01
TAATACG502.1827873E-1145.01
GTCGCGC207.030192E-445.021
CTCACGA207.030192E-445.024
ACCGGTA207.030192E-445.041
GTCGCAC253.8882397E-545.012
TCCGCTA253.8882397E-545.031
Produced by FastQC (version 0.11.5)