##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934962.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560571
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53443363998495	34.0	31.0	34.0	31.0	34.0
2	32.69629716842291	34.0	31.0	34.0	31.0	34.0
3	32.76109538310045	34.0	31.0	34.0	31.0	34.0
4	36.16831231012664	37.0	37.0	37.0	35.0	37.0
5	36.0590201776403	37.0	35.0	37.0	35.0	37.0
6	36.04001455658605	37.0	35.0	37.0	35.0	37.0
7	36.20164617862858	37.0	37.0	37.0	35.0	37.0
8	36.20080239612823	37.0	37.0	37.0	35.0	37.0
9	38.018780850240205	39.0	39.0	39.0	35.0	39.0
10	37.51329804788332	39.0	37.0	39.0	34.0	39.0
11	37.43757347418971	39.0	37.0	39.0	35.0	39.0
12	37.31085981971954	39.0	37.0	39.0	35.0	39.0
13	37.196663758917246	39.0	37.0	39.0	34.0	39.0
14	38.451022261230065	40.0	38.0	41.0	34.0	41.0
15	38.52287043032908	40.0	38.0	41.0	34.0	41.0
16	38.632414092059705	40.0	38.0	41.0	35.0	41.0
17	38.678395778590044	40.0	38.0	41.0	35.0	41.0
18	38.204839351304294	39.0	38.0	40.0	35.0	41.0
19	37.701017355517855	38.0	37.0	40.0	35.0	41.0
20	37.11918383219967	38.0	35.0	40.0	34.0	41.0
21	37.053158297521634	38.0	35.0	40.0	34.0	41.0
22	36.88129603564936	37.0	35.0	40.0	34.0	41.0
23	36.778128015898076	37.0	35.0	40.0	33.0	41.0
24	36.56417295935751	37.0	35.0	40.0	33.0	41.0
25	36.38448831637741	36.0	35.0	40.0	33.0	41.0
26	36.52401033945745	37.0	35.0	40.0	33.0	41.0
27	36.56884498127802	37.0	35.0	40.0	33.0	41.0
28	36.51001211264942	37.0	35.0	40.0	33.0	41.0
29	36.37164426986055	37.0	35.0	40.0	33.0	41.0
30	36.049881281764485	37.0	35.0	40.0	32.0	41.0
31	35.45135763355579	37.0	35.0	40.0	30.0	41.0
32	34.63109400950103	37.0	35.0	40.0	21.0	41.0
33	33.704774239124035	37.0	34.0	40.0	15.0	41.0
34	32.93211743026307	37.0	33.0	40.0	10.0	41.0
35	32.444775059715894	37.0	32.0	40.0	8.0	41.0
36	32.243014711784944	37.0	31.0	40.0	8.0	41.0
37	32.05526151013877	37.0	31.0	40.0	8.0	41.0
38	31.931448469506986	37.0	30.0	40.0	8.0	41.0
39	31.880705566288658	37.0	30.0	40.0	7.0	41.0
40	31.685126415743948	36.0	30.0	40.0	7.0	41.0
41	31.542436194523084	36.0	29.0	40.0	7.0	41.0
42	31.4274088384879	36.0	29.0	40.0	7.0	41.0
43	31.333270896996098	36.0	28.0	40.0	7.0	41.0
44	31.20506233822299	36.0	27.0	40.0	7.0	41.0
45	31.118755697315773	36.0	26.0	40.0	7.0	41.0
46	30.983234951504805	35.0	26.0	40.0	7.0	41.0
47	30.906522099787537	35.0	25.0	40.0	7.0	41.0
48	30.858271298372554	35.0	25.0	40.0	7.0	41.0
49	30.751706741875694	35.0	25.0	40.0	7.0	41.0
50	30.65542634206907	35.0	24.0	40.0	7.0	41.0
51	29.979792033480148	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	1.0
9	8.0
10	4.0
11	6.0
12	6.0
13	9.0
14	11.0
15	18.0
16	52.0
17	102.0
18	196.0
19	370.0
20	692.0
21	1114.0
22	1900.0
23	3394.0
24	7169.0
25	14969.0
26	24992.0
27	29248.0
28	25204.0
29	18722.0
30	14632.0
31	13064.0
32	13610.0
33	17483.0
34	32497.0
35	47630.0
36	37961.0
37	50857.0
38	92977.0
39	111601.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0343007397814	14.48094888961434	17.87088522238931	35.61386514821495
2	50.3691771425921	18.30990186791682	17.261149791908608	14.059771197582466
3	23.586307532854892	18.92338347863161	43.8852527155347	13.605056272978802
4	21.312911299371535	19.532762130042403	44.81305668684252	14.341269883743541
5	18.94853640306045	22.442117055645046	43.49511480258522	15.11423173870928
6	19.820147670857036	25.92909729543626	43.07322355241352	11.177531481293181
7	59.627772396360136	4.502016693692681	31.868755251341934	4.001455658605243
8	59.60493853588573	4.124722827260062	31.711237291975504	4.559101344878704
9	56.09565960422498	4.96565109504416	33.37793071707241	5.5607585836584486
10	30.47678171007776	17.94081392009219	39.51738495212917	12.06501941770088
11	24.414391754122136	18.764081623915615	42.401230174233056	14.420296447729191
12	21.62491459601014	17.516960385035972	45.72088103023525	15.137243988718646
13	16.995527774358646	22.53702028824181	47.022767856346476	13.444684081053069
14	14.59779403501073	24.192296783101515	45.85271089656796	15.35719828531979
15	12.49904115624961	19.968210984870783	52.87947467849746	14.653273180382145
16	14.424399407033187	19.13388312988007	49.89448259007334	16.54723487301341
17	15.455490919080722	18.23087530393117	45.48094710571899	20.832686671269116
18	16.81874374521693	18.869331449539846	47.930592199739195	16.38133260550403
19	17.029421786000345	20.57990870023601	45.67646203603112	16.714207477732526
20	18.60727722268901	19.841019246446926	46.497053896830195	15.05464963403387
21	16.151745274015244	21.20926697956191	47.1827832692023	15.456204477220547
22	15.046800494495791	20.02137106628777	45.19356156490436	19.738266874312085
23	14.718385360641204	20.85177435150944	45.40227732080325	19.0275629670461
24	16.085027587941582	19.065203158921882	47.618232124030676	17.23153712910586
25	13.76417973815984	21.81347233445897	46.20574378624652	18.216604141134667
26	13.98484759290081	22.650119253404117	45.657731134860704	17.707302018834365
27	15.655822366836672	21.902131933332264	46.348990582816455	16.093055117014615
28	12.95589675527275	22.014160561284832	48.734237054717426	16.295705628724996
29	13.979674296387076	20.87514338058872	47.492824280956384	17.65235804206782
30	16.051311965834834	22.094079072945263	44.587037146052865	17.267571815167035
31	17.51267903619702	24.69071714376948	41.102732749286	16.693871070747505
32	17.295935751225088	24.37728673085122	41.51320706922049	16.813570448703196
33	17.318948001234453	23.49532887002717	38.057444998046634	21.12827813069174
34	16.862984349886098	24.94902519038623	38.04138993990057	20.146600519827103
35	17.155543187214466	25.547700469699645	36.32456905548093	20.97218728760496
36	20.659648822361483	26.18651339437823	34.02316566500943	19.130672118250853
37	18.302944676053524	27.035469191235368	36.622836357927895	18.03874977478321
38	16.01277982628427	28.838452221038906	34.45326283378912	20.695505118887706
39	18.62315389130012	25.27387253354169	35.25405345620805	20.848920118950144
40	20.483399961824638	24.704453137961117	34.682314996673036	20.12983190354121
41	18.496497321481133	26.25394463859172	32.47028476321465	22.779273276712495
42	19.051288775195292	25.847216498891306	32.92678358316788	22.174711142745522
43	20.359062455960082	24.538015701846867	33.963226781264105	21.139695060928947
44	17.32715391984245	26.152976161806446	34.277370752322184	22.242499166028924
45	18.198586798104078	25.405167231269544	33.760933048623635	22.635312922002743
46	20.4921410490375	25.400172324290764	34.10201383946012	20.005672787211612
47	17.439717716399887	25.274229312611606	36.69026760214138	20.59578536884712
48	17.29201118145605	24.227617911022868	35.960297625100125	22.52007328242096
49	18.138647914358753	21.97027673568558	37.55206744551538	22.339007904440294
50	17.630237739733236	23.333707951356743	36.625333811417285	22.410720497492733
51	16.799477675441647	23.306057573438512	33.806243990502544	26.088220760617297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	585.0
1	686.5
2	788.0
3	17085.5
4	33383.0
5	23368.0
6	13353.0
7	13403.0
8	13453.0
9	13898.0
10	14343.0
11	14350.5
12	14358.0
13	13615.0
14	12872.0
15	12091.0
16	11310.0
17	10319.0
18	9328.0
19	8415.0
20	7502.0
21	6926.5
22	6351.0
23	5692.0
24	5033.0
25	5080.0
26	4890.5
27	4654.0
28	4930.0
29	5206.0
30	5726.0
31	6246.0
32	7273.5
33	8301.0
34	8493.0
35	8685.0
36	9991.5
37	11298.0
38	12217.0
39	13136.0
40	15964.0
41	18792.0
42	23417.0
43	28042.0
44	31440.5
45	34839.0
46	40954.5
47	47070.0
48	50132.5
49	53195.0
50	51520.0
51	49845.0
52	41577.0
53	33309.0
54	28331.0
55	23353.0
56	20766.5
57	18180.0
58	15986.5
59	13793.0
60	12126.5
61	10460.0
62	9302.0
63	8144.0
64	7048.0
65	5952.0
66	4934.0
67	3916.0
68	3128.5
69	2341.0
70	1987.0
71	1633.0
72	1344.0
73	1055.0
74	873.0
75	503.5
76	316.0
77	262.5
78	209.0
79	135.5
80	62.0
81	41.0
82	20.0
83	18.0
84	16.0
85	11.5
86	7.0
87	11.0
88	15.0
89	8.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.93383610792814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98043817451608	27.662024488795556
2	10.251048603560871	7.36719000909524
3	3.9855680807926968	4.296502506365833
4	2.070318928383763	2.975780042547342
5	1.1861335055394524	2.1311163495088477
6	0.7395798088464552	1.5945563783892804
7	0.4653765235634507	1.170593460834448
8	0.3460811943561863	0.9948819934424977
9	0.2763539061964213	0.8937410375743153
>10	2.981105927011934	27.08712980877962
>50	0.6653727143135907	15.175283323193456
>100	0.0465507906591955	3.249677567729491
>500	0.004047894839930044	1.0820137193284955
>1k	0.0015179605649737662	1.1156505094605358
>5k	0.0	0.0
>10k+	5.059868549912555E-4	3.2038588049550363
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17621	3.1434019954653376	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2444	0.43598402343324927	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2311	0.4122582152840586	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	1381	0.24635594777467976	TruSeq Adapter, Index 14 (95% over 23bp)
GCCGAAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCTT	980	0.1748217442571949	No Hit
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	931	0.16608065704433517	TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	887	0.158231517506257	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	797	0.1421764593601881	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG	654	0.11666675586143416	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	624	0.1113150698127445	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26722752336456934	0.0
2	0.0	0.0	0.0	1.476173401763559	0.0
3	0.0	0.0	0.0	2.111775314812932	0.0
4	0.0	0.0	0.0	2.7584373790295964	0.0
5	0.0	0.0	0.0	4.3939126355091505	0.0
6	0.0	0.0	0.0	5.155100781167773	0.0
7	0.0	0.0	0.0	5.957318519866351	0.0
8	0.0	0.0	0.0	7.126661921505037	0.0
9	0.0	0.0	0.0	7.530714218181105	0.0
10	0.0	0.0	0.0	8.621031055834141	0.0
11	0.0	0.0	0.0	10.171949672744399	0.0
12	0.0	0.0	0.0	11.449753911636527	0.0
13	0.0	0.0	0.0	11.895906138562287	0.0
14	0.0	0.0	0.0	12.062521964211491	0.0
15	0.0	0.0	0.0	12.433393807385684	0.0
16	0.0	0.0	0.0	13.19440356350935	0.0
17	0.0	0.0	0.0	14.180362523212938	0.0
18	0.0	0.0	0.0	15.39305458184601	0.0
19	0.0	0.0	0.0	15.987270122785517	0.0
20	1.7838953495632134E-4	0.0	0.0	16.542418355569588	0.0
21	1.7838953495632134E-4	0.0	0.0	17.278275187264413	0.0
22	1.7838953495632134E-4	0.0	0.0	17.97774055382815	0.0
23	1.7838953495632134E-4	0.0	0.0	18.724300757620355	0.0
24	1.7838953495632134E-4	0.0	0.0	19.302282850878836	0.0
25	1.7838953495632134E-4	0.0	0.0	19.791783734798983	0.0
26	1.7838953495632134E-4	0.0	0.0	20.271651583831485	0.0
27	1.7838953495632134E-4	0.0	0.0	20.741172839836523	0.0
28	1.7838953495632134E-4	0.0	0.0	21.217651287704857	0.0
29	1.7838953495632134E-4	0.0	0.0	21.716428427442732	0.0
30	1.7838953495632134E-4	0.0	0.0	22.28584782302331	0.0
31	3.5677906991264267E-4	0.0	0.0	22.79996646276743	0.0
32	3.5677906991264267E-4	0.0	0.0	23.271806782726898	0.0
33	3.5677906991264267E-4	0.0	0.0	23.763805120136432	0.0
34	3.5677906991264267E-4	0.0	0.0	24.217449707530356	0.0
35	3.5677906991264267E-4	0.0	0.0	24.77348988798921	0.0
36	3.5677906991264267E-4	0.0	0.0	25.227312864918094	0.0
37	3.5677906991264267E-4	0.0	0.0	25.629759655779555	0.0
38	3.5677906991264267E-4	0.0	0.0	26.076447051310183	0.0
39	3.5677906991264267E-4	0.0	0.0	26.495662458457538	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	30	2.1635042E-6	45.000004	1
CGAACTA	30	2.1635042E-6	45.000004	29
CACGACC	35	1.2104465E-7	45.000004	27
TACGCCG	30	2.1635042E-6	45.000004	3
CCGAGGT	35	1.2104465E-7	45.000004	7
TTACGAG	30	2.1635042E-6	45.000004	1
CGAGACC	30	2.1635042E-6	45.000004	36
AGTAGCG	30	2.1635042E-6	45.000004	1
CCTATCC	30	2.1635042E-6	45.000004	22
TACAGCG	30	2.1635042E-6	45.000004	1
ACGAGAC	35	1.2104465E-7	45.000004	35
TACACGC	65	0.0	45.000004	35
TCGCCAA	20	7.030192E-4	45.0	19
CTTAACG	25	3.8882397E-5	45.0	1
TAATACG	50	2.1827873E-11	45.0	1
GTCGCGC	20	7.030192E-4	45.0	21
CTCACGA	20	7.030192E-4	45.0	24
ACCGGTA	20	7.030192E-4	45.0	41
GTCGCAC	25	3.8882397E-5	45.0	12
TCCGCTA	25	3.8882397E-5	45.0	31
>>END_MODULE