##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934960.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	979761
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.438768230211245	34.0	31.0	34.0	30.0	34.0
2	32.62344796332983	34.0	31.0	34.0	31.0	34.0
3	32.688835338414165	34.0	31.0	34.0	31.0	34.0
4	36.114817797401614	37.0	37.0	37.0	35.0	37.0
5	35.987129514238674	37.0	35.0	37.0	35.0	37.0
6	35.97287501747875	37.0	35.0	37.0	35.0	37.0
7	36.17371379346596	37.0	36.0	37.0	35.0	37.0
8	36.19923634437378	37.0	37.0	37.0	35.0	37.0
9	37.9969982475318	39.0	39.0	39.0	35.0	39.0
10	37.46913584027125	39.0	37.0	39.0	34.0	39.0
11	37.33470101381867	39.0	37.0	39.0	34.0	39.0
12	37.35281257367868	39.0	37.0	39.0	34.0	39.0
13	37.34848498766536	39.0	37.0	39.0	34.0	39.0
14	38.58673492821208	40.0	38.0	41.0	34.0	41.0
15	38.63654809693384	40.0	38.0	41.0	34.0	41.0
16	38.736599027722065	40.0	38.0	41.0	35.0	41.0
17	38.76639915244636	40.0	38.0	41.0	35.0	41.0
18	38.30458550605709	39.0	38.0	40.0	35.0	41.0
19	37.82351614322268	38.0	37.0	40.0	34.0	41.0
20	37.26816029623551	38.0	35.0	40.0	34.0	41.0
21	37.22889357710707	38.0	35.0	40.0	34.0	41.0
22	37.069352627834746	38.0	35.0	40.0	34.0	41.0
23	36.93110360587939	38.0	35.0	40.0	33.0	41.0
24	36.69748744846957	38.0	35.0	40.0	33.0	41.0
25	36.57060140177043	37.0	35.0	40.0	33.0	41.0
26	36.75920658201337	38.0	35.0	40.0	33.0	41.0
27	36.830061617067834	38.0	35.0	40.0	33.0	41.0
28	36.75088720616559	38.0	35.0	40.0	33.0	41.0
29	36.616421760000655	38.0	35.0	40.0	33.0	41.0
30	36.28186567948714	38.0	35.0	40.0	32.0	41.0
31	35.708836134526685	38.0	35.0	40.0	30.0	41.0
32	34.95255985898602	38.0	35.0	40.0	23.0	41.0
33	33.9503940246652	38.0	34.0	40.0	16.0	41.0
34	33.162873394634	38.0	33.0	40.0	10.0	41.0
35	32.724000036743654	38.0	33.0	40.0	8.0	41.0
36	32.51695974834679	38.0	32.0	40.0	8.0	41.0
37	32.32815145734521	38.0	31.0	40.0	8.0	41.0
38	32.20069996662451	38.0	31.0	40.0	8.0	41.0
39	32.16922902626253	38.0	31.0	40.0	8.0	41.0
40	32.098641403362656	38.0	30.0	40.0	8.0	41.0
41	32.012052939441354	38.0	30.0	40.0	7.0	41.0
42	31.901409629491273	38.0	30.0	40.0	7.0	41.0
43	31.782325485501055	38.0	29.0	40.0	7.0	41.0
44	31.657638954806327	37.0	29.0	40.0	7.0	41.0
45	31.592468979679737	37.0	28.0	40.0	7.0	41.0
46	31.478218667613838	37.0	27.0	40.0	7.0	41.0
47	31.39909222759428	37.0	27.0	40.0	7.0	41.0
48	31.333896736040728	37.0	27.0	40.0	7.0	41.0
49	31.21685390620774	37.0	27.0	40.0	7.0	41.0
50	31.102439268352178	36.0	26.0	40.0	7.0	41.0
51	30.49329275200789	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	3.0
11	2.0
12	10.0
13	10.0
14	13.0
15	32.0
16	51.0
17	150.0
18	311.0
19	657.0
20	1099.0
21	1865.0
22	3073.0
23	5789.0
24	12604.0
25	26570.0
26	44694.0
27	48847.0
28	40180.0
29	30139.0
30	23652.0
31	21620.0
32	23000.0
33	29015.0
34	47900.0
35	64616.0
36	68829.0
37	99702.0
38	183657.0
39	201550.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.391329109854343	16.302343122455373	18.086247564457047	34.22008020323324
2	50.341869088481786	18.054709260727872	17.79209419440047	13.811327456389874
3	23.524818807852117	17.84414770540979	45.02169406620594	13.609339420532148
4	21.773269195242513	20.290560657139853	44.36000208214044	13.576168065477193
5	19.25010283120067	22.31135960708785	43.95949624449228	14.479041317219199
6	20.545316663961927	24.764917158368213	43.04325238502043	11.646513792649433
7	59.962582711498015	4.238992978899956	31.51952363892827	4.2789006706737664
8	59.842349307637264	3.402258305852141	31.295081147341037	5.4603112391695525
9	55.262865127311656	5.563907932648881	33.31343052030036	5.8597964197391
10	27.377697213912374	22.92855094252578	39.08626695694154	10.607484886620307
11	19.834224877291504	18.908693038404266	46.062764286392294	15.19431779791194
12	18.459195660982626	17.520497345781266	49.37377584941633	14.646531143819768
13	17.009046083687757	19.87964411728983	49.62444922792395	13.486860571098461
14	14.388304902930408	22.541007449776014	46.98492795692011	16.085759690373468
15	13.342641725890294	21.00410202079895	50.90761930715756	14.745636946153196
16	15.732510275465138	20.556237694703096	47.85309886798924	15.85815316184253
17	16.074124199677268	19.47924034534953	45.642763898542604	18.8038715564306
18	15.976957645793208	19.91026382964825	48.394659513901864	15.71811901065668
19	15.088271527443938	21.14709607751278	46.53849255073431	17.226139844308967
20	16.67406643048662	21.655893631201895	47.093219672961055	14.576820265350426
21	15.668310945220314	21.46829686015263	48.021915548792	14.841476645835055
22	14.804835056712811	20.41365190082071	45.48160214582944	19.299910896637037
23	13.314471590520546	21.75336638220954	45.95947379003655	18.972688237233363
24	15.468160092104094	19.937005045107938	46.77957175270295	17.815263110085013
25	13.416231101258369	21.719684698615275	45.79229016055957	19.071794039566793
26	12.875691112424356	21.343266368022405	47.835645631944935	17.945396887608304
27	14.352173642347472	21.74469079704132	46.3195616073716	17.58357395323962
28	12.093153330250948	21.777147692141245	47.54087986764119	18.588819109966614
29	13.07257586288901	20.70219165694491	47.47331236903694	18.75192011112914
30	13.85521571077028	21.536068490172603	44.63619188761341	19.97252391144371
31	14.245617043340161	24.148236151469593	41.294866809354524	20.31127999583572
32	15.568898945763305	24.70867895333658	40.34208342646829	19.380338674431826
33	15.511027689405884	24.488931484310967	37.828817436089004	22.17122339019414
34	14.83096387792533	24.60130582866638	38.61533578086901	21.952394512539282
35	16.450848727393723	24.989053452831865	35.77545952533322	22.784638294441194
36	17.265639273251335	26.24558438231365	34.936989735251764	21.55178660918326
37	16.467587503482992	27.712166538574202	35.432110484087445	20.38813547385536
38	16.831962080548216	29.58415368646027	32.65551496742573	20.928369265565784
39	17.264924813296304	26.7891863423835	34.53566737194071	21.41022147237949
40	18.886340648382614	23.37906897702603	35.476304935591436	22.25828543899992
41	18.16636914512825	25.23697105722722	32.51619527619491	24.080464521449617
42	18.607701265920976	25.18328449489212	34.62630172052164	21.582712518665268
43	18.583001364618514	25.88263872515848	33.91347481681757	21.62088509340543
44	16.735305855203464	25.561437942518634	34.036872257622015	23.66638394465589
45	17.637464646990438	24.120066016099845	33.367423279759045	24.875046057150673
46	19.018924002894583	24.093426866348018	34.24733174723224	22.640317383525165
47	15.833249129124347	24.911993843396502	36.92706690713348	22.327690120345675
48	16.64885620064485	23.539005941244852	35.75994553773828	24.052192320372008
49	17.837819631522382	22.53059674757415	37.4162678449132	22.215315775990266
50	17.23348857527499	22.170304798823388	36.3166119084144	24.279594717487225
51	16.739796746349363	21.58608068702469	33.361095205871635	28.313027360754305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	528.0
1	704.0
2	880.0
3	26396.0
4	51912.0
5	36821.0
6	21730.0
7	21962.0
8	22194.0
9	22917.0
10	23640.0
11	23826.0
12	24012.0
13	23120.0
14	22228.0
15	20925.0
16	19622.0
17	18027.5
18	16433.0
19	15172.5
20	13912.0
21	12899.5
22	11887.0
23	10963.0
24	10039.0
25	9884.0
26	9184.0
27	8639.0
28	9551.0
29	10463.0
30	10232.0
31	10001.0
32	11790.5
33	13580.0
34	16538.0
35	19496.0
36	20473.0
37	21450.0
38	25522.0
39	29594.0
40	33296.0
41	36998.0
42	41604.0
43	46210.0
44	53818.5
45	61427.0
46	73536.5
47	85646.0
48	95482.0
49	105318.0
50	102482.5
51	99647.0
52	81663.5
53	63680.0
54	52133.0
55	40586.0
56	33019.5
57	25453.0
58	21598.0
59	17743.0
60	15186.5
61	12630.0
62	10923.5
63	9217.0
64	7715.5
65	6214.0
66	4631.0
67	3048.0
68	2410.5
69	1773.0
70	1358.0
71	943.0
72	722.0
73	501.0
74	429.0
75	270.0
76	183.0
77	152.5
78	122.0
79	71.5
80	21.0
81	20.5
82	20.0
83	33.5
84	47.0
85	26.5
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	979761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.51766262298085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95602584500267	26.62262837522238
2	10.530412430291584	7.480312719598807
3	4.481859364068555	4.775555064499033
4	2.389692116914172	3.395051135254178
5	1.4640233850835076	2.5999344331775203
6	0.965074345884852	2.056631101393926
7	0.6511380255989503	1.6188830499952167
8	0.4741869156578168	1.347360871245297
9	0.3442490056190375	1.100422803588625
>10	3.1393781640529093	26.110113726608326
>50	0.5266045944731597	11.960451419358384
>100	0.06976618097106173	4.416835457050896
>500	0.004670539311816609	1.1172465173320152
>1k	0.0020433609489197663	1.3285446244517858
>5k	5.838174139770761E-4	1.1377749889756437
>10k+	2.9190870698853804E-4	2.9322537122479635
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28282	2.8866223497363133	No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	5505	0.5618717217770457	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	5469	0.5581973562940349	No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	3535	0.3608022772900738	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	2278	0.23250568250828518	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1967	0.2007632473633876	No Hit
ACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	1587	0.16197827837605294	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	1385	0.14136100538804872	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1059	0.10808758462523002	No Hit
AGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1003	0.10237190498499124	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0413141572281403E-4	0.0	0.0	0.38519598146895007	0.0
2	2.0413141572281403E-4	0.0	0.0	2.0125316276112235	0.0
3	2.0413141572281403E-4	0.0	0.0	2.8994826289268505	0.0
4	2.0413141572281403E-4	0.0	0.0	3.7771456508270895	0.0
5	2.0413141572281403E-4	0.0	0.0	5.870819516188131	0.0
6	2.0413141572281403E-4	0.0	0.0	6.78471586437917	0.0
7	2.0413141572281403E-4	0.0	0.0	7.835788523935939	0.0
8	2.0413141572281403E-4	0.0	0.0	9.480883603246097	0.0
9	2.0413141572281403E-4	0.0	0.0	10.069088277651387	0.0
10	2.0413141572281403E-4	0.0	0.0	11.466980212521216	0.0
11	2.0413141572281403E-4	0.0	0.0	13.61587162583528	0.0
12	2.0413141572281403E-4	0.0	0.0	15.29372979736895	0.0
13	2.0413141572281403E-4	0.0	0.0	15.896325736582698	0.0
14	2.0413141572281403E-4	0.0	0.0	16.125361185023696	0.0
15	2.0413141572281403E-4	0.0	0.0	16.581594899164184	0.0
16	2.0413141572281403E-4	0.0	0.0	17.53815471324129	0.0
17	2.0413141572281403E-4	0.0	0.0	18.765698981690434	0.0
18	2.0413141572281403E-4	0.0	0.0	20.189311474941338	0.0
19	2.0413141572281403E-4	0.0	0.0	21.011450751764972	0.0
20	2.0413141572281403E-4	0.0	0.0	21.778372480635582	0.0
21	2.0413141572281403E-4	0.0	0.0	22.66583381049052	0.0
22	2.0413141572281403E-4	0.0	0.0	23.621168836073288	0.0
23	2.0413141572281403E-4	0.0	0.0	24.50863016592822	0.0
24	2.0413141572281403E-4	0.0	0.0	25.198798482487057	0.0
25	2.0413141572281403E-4	0.0	0.0	25.793433296487613	0.0
26	2.0413141572281403E-4	0.0	0.0	26.398682944105758	0.0
27	2.0413141572281403E-4	0.0	0.0	26.966372411230903	0.0
28	2.0413141572281403E-4	0.0	0.0	27.533653615524603	0.0
29	2.0413141572281403E-4	0.0	0.0	28.12267481559278	0.0
30	2.0413141572281403E-4	0.0	0.0	28.784264733950423	0.0
31	3.0619712358422106E-4	0.0	0.0	29.393801141298745	0.0
32	4.0826283144562806E-4	0.0	0.0	29.987211166804965	0.0
33	4.0826283144562806E-4	0.0	0.0	30.525199512942443	0.0
34	4.0826283144562806E-4	0.0	0.0	31.06410645045067	0.0
35	4.0826283144562806E-4	0.0	0.0	31.62414098948621	0.0
36	4.0826283144562806E-4	0.0	0.0	32.15131037059038	0.0
37	4.0826283144562806E-4	0.0	0.0	32.66163890989741	0.0
38	4.0826283144562806E-4	0.0	0.0	33.170844726417975	0.0
39	4.0826283144562806E-4	0.0	0.0	33.67178322060176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.0327753E-4	45.000004	1
AGCCGAC	20	7.0327753E-4	45.000004	19
TCGTTCC	20	7.0327753E-4	45.000004	16
AAATCGA	35	1.2116834E-7	45.000004	28
TTCAGCG	45	3.8380676E-10	45.000004	1
CTCCGCG	20	7.0327753E-4	45.000004	1
GCGCGAT	20	7.0327753E-4	45.000004	1
CGGCCAA	20	7.0327753E-4	45.000004	30
ATTACGA	20	7.0327753E-4	45.000004	18
GCTATCG	40	6.8139343E-9	45.000004	2
GGTCCGT	20	7.0327753E-4	45.000004	8
CGACCCG	20	7.0327753E-4	45.000004	32
CATTCGG	20	7.0327753E-4	45.000004	21
ATCACGC	20	7.0327753E-4	45.000004	13
ACCCTCG	20	7.0327753E-4	45.000004	15
CATTACG	20	7.0327753E-4	45.000004	17
ATCCGCC	20	7.0327753E-4	45.000004	13
CTAACGG	40	6.8139343E-9	45.000004	2
GCCGAGT	20	7.0327753E-4	45.000004	13
CCGTAAC	20	7.0327753E-4	45.000004	11
>>END_MODULE