SRR2934959.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934959.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	894722
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34512	3.8572875150046606	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3587	0.40090665033384665	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3452	0.38581816474837993	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	2046	0.22867438153974085	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1296	0.144849461620481	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC	1064	0.11891961972545662	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTG	965	0.10785473029611431	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	952	0.1064017650175138	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	917	0.1024899354212817	TruSeq Adapter, Index 21 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	130	0.0	45.000004	6
GCATAAG	25	3.89011E-5	45.0	1
CTATGCG	20	7.032449E-4	45.0	1
ACGCCCC	20	7.032449E-4	45.0	40
GGTCGAT	25	3.89011E-5	45.0	19
CGTGCAC	20	7.032449E-4	45.0	20
GAGTACG	20	7.032449E-4	45.0	1
ATTAACG	20	7.032449E-4	45.0	34
CGTCTAA	25	3.89011E-5	45.0	43
CGCGATT	20	7.032449E-4	45.0	43
CGATTCG	40	6.8121153E-9	45.0	10
ACGGGTC	55	1.8189894E-12	45.0	5
CTGCGTA	20	7.032449E-4	45.0	16
TAGCACG	20	7.032449E-4	45.0	1
CCGTATC	20	7.032449E-4	45.0	32
CCGTATA	25	3.89011E-5	45.0	30
CTAACGG	25	3.89011E-5	45.0	2
TACGAAT	55	1.8189894E-12	45.0	12
TATTCGA	20	7.032449E-4	45.0	14
AACGCTA	20	7.032449E-4	45.0	27