##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934959.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	894722
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.510826826656775	34.0	31.0	34.0	31.0	34.0
2	32.676800168096904	34.0	31.0	34.0	31.0	34.0
3	32.73931679337269	34.0	31.0	34.0	31.0	34.0
4	36.14925977007383	37.0	37.0	37.0	35.0	37.0
5	36.03284819195236	37.0	35.0	37.0	35.0	37.0
6	36.02964160934905	37.0	35.0	37.0	35.0	37.0
7	36.20044438384213	37.0	37.0	37.0	35.0	37.0
8	36.2065010137227	37.0	37.0	37.0	35.0	37.0
9	37.96891548436274	39.0	39.0	39.0	35.0	39.0
10	37.558756798201	39.0	37.0	39.0	35.0	39.0
11	37.42013385163213	39.0	37.0	39.0	34.0	39.0
12	37.28695281886441	39.0	37.0	39.0	34.0	39.0
13	37.19686450092878	39.0	37.0	39.0	34.0	39.0
14	38.42046132765261	40.0	38.0	41.0	34.0	41.0
15	38.44812131589477	40.0	38.0	41.0	34.0	41.0
16	38.550298304948356	40.0	38.0	41.0	34.0	41.0
17	38.563503523999636	40.0	38.0	41.0	34.0	41.0
18	38.04307594984811	39.0	37.0	40.0	34.0	41.0
19	37.60861362523778	38.0	37.0	40.0	34.0	41.0
20	37.09340778476443	38.0	35.0	40.0	34.0	41.0
21	37.05067719358639	38.0	35.0	40.0	34.0	41.0
22	36.84933532426832	38.0	35.0	40.0	33.0	41.0
23	36.736931694984584	37.0	35.0	40.0	33.0	41.0
24	36.557749781496376	37.0	35.0	40.0	33.0	41.0
25	36.44552833170527	37.0	35.0	40.0	33.0	41.0
26	36.57837741779011	37.0	35.0	40.0	33.0	41.0
27	36.6313357668639	37.0	35.0	40.0	33.0	41.0
28	36.580347862241005	37.0	35.0	40.0	33.0	41.0
29	36.48195193590858	37.0	35.0	40.0	33.0	41.0
30	36.17178520255454	37.0	35.0	40.0	32.0	41.0
31	35.6424397745892	37.0	35.0	40.0	30.0	41.0
32	34.948705855003006	37.0	35.0	40.0	23.0	41.0
33	34.08729974226631	37.0	34.0	40.0	18.0	41.0
34	33.388467032217825	37.0	33.0	40.0	14.0	41.0
35	32.93587952458976	37.0	33.0	40.0	10.0	41.0
36	32.75829922590481	37.0	33.0	40.0	10.0	41.0
37	32.56145260762561	37.0	32.0	40.0	10.0	41.0
38	32.457656121119186	37.0	32.0	40.0	10.0	41.0
39	32.44907356698505	37.0	32.0	40.0	10.0	41.0
40	32.33641846294156	37.0	31.0	40.0	9.0	41.0
41	32.22123072865091	37.0	31.0	40.0	8.0	41.0
42	32.11359841380898	37.0	31.0	40.0	8.0	41.0
43	32.02037727919957	36.0	31.0	40.0	8.0	41.0
44	31.908592836657643	36.0	30.0	40.0	8.0	41.0
45	31.858623125395376	36.0	30.0	40.0	8.0	41.0
46	31.78044353441628	36.0	30.0	40.0	8.0	41.0
47	31.732854450879714	36.0	30.0	40.0	8.0	41.0
48	31.680781292960273	35.0	30.0	40.0	8.0	41.0
49	31.56067024170636	35.0	29.0	40.0	8.0	41.0
50	31.459553917306156	35.0	29.0	40.0	8.0	41.0
51	30.894877962093254	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	11.0
11	10.0
12	9.0
13	16.0
14	19.0
15	36.0
16	77.0
17	165.0
18	329.0
19	577.0
20	986.0
21	1538.0
22	2497.0
23	4340.0
24	8818.0
25	18816.0
26	33010.0
27	40279.0
28	37808.0
29	31639.0
30	26663.0
31	24168.0
32	23949.0
33	30055.0
34	57275.0
35	69748.0
36	63969.0
37	79722.0
38	149851.0
39	188201.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.548371449455807	16.026654089203127	18.057340715887168	35.367633745453894
2	50.28567532708483	17.30995772988705	18.771417267039368	13.632949675988742
3	23.54139051012493	17.134707763975847	45.987133433625196	13.336768292274026
4	22.68626456038859	19.15377066843109	44.32896475106234	13.831000020117981
5	18.936831775680044	22.506096865842125	43.3424013268926	15.214670031585229
6	21.110803132146074	24.86504187892999	42.07318027275511	11.950974716168822
7	60.41172565333143	4.442720755720772	31.037014849305145	4.108538741642655
8	60.529751140577744	3.879081994183668	30.837846839576986	4.753320025661602
9	55.82124950543297	5.854444173720999	32.882392519687684	5.441913801158348
10	32.96554684024758	18.39454042708238	38.02220130945702	10.61771142321302
11	26.24725892511864	17.473695740129337	41.42236359450198	14.856681740250044
12	24.625079074841125	15.145486531011867	45.40952385210154	14.819910542045461
13	20.372026171257666	18.37129298262477	46.072858385062624	15.183822461054941
14	15.605517691528764	20.960812408770547	45.68882848527252	17.74484141442817
15	15.001419435310634	19.176123980409557	50.75844787542946	15.064008708850347
16	17.568585549477937	18.302444781731086	48.08610942840346	16.042860240387515
17	17.376123533343318	18.706704428861702	44.92479228184844	18.99237975594654
18	16.719494993975783	20.102333462237432	47.201253573735755	15.976917970051034
19	18.60421449344042	20.419862258891587	44.65364660754961	16.322276640118382
20	20.154751978826944	20.96964196700204	44.80140199972729	14.074204054443726
21	19.097999155044807	20.12692210541375	46.08269384233315	14.692384897208294
22	17.26279224161248	20.019626207917096	44.45436683126155	18.26321471920887
23	17.245803724508843	19.76211605392513	45.73968227002354	17.252397951542488
24	18.64556812060059	17.752776840180527	45.9491328032618	17.65252223595709
25	16.828690922990607	19.24821341154012	44.65979376834369	19.263301897125586
26	15.045120160228539	20.52525812487007	44.88109155693053	19.548530157970855
27	15.808262231173481	20.250088854415115	46.42168181848664	17.51996709592477
28	13.296308797592996	20.28943068349722	47.454516598451804	18.959743920457974
29	15.610658953283812	19.05809849316324	46.61246733622287	18.718775217330077
30	18.05231122069201	19.357968173354408	45.25416833385118	17.335552272102394
31	18.362575190953166	21.29354145757006	43.03604918622768	17.307834165249094
32	18.877483732377208	21.300359217723493	41.93347207288968	17.88868497700962
33	19.115881804627584	22.391647908512365	40.08507670539005	18.40739358147
34	17.767530026086316	23.22073224979379	39.874843806232555	19.136893917887342
35	17.235856500678423	24.196566084213867	37.7536262660357	20.813951149072
36	18.710504491898043	25.30663155706465	36.82719325108805	19.15567069994926
37	18.424717398253314	26.103303595977295	38.353700926097716	17.118278079671676
38	18.209455003900654	26.550928668346145	35.87237152992773	19.36724479782547
39	20.4999988823344	23.776212052458753	35.836829763881966	19.88695930132488
40	20.871510927416562	23.562514389944585	35.95865531416462	19.60731936847423
41	17.001929090823744	25.21889480754916	34.51194896291809	23.267227138709007
42	17.13336656525714	24.43820538670112	35.21540769088052	23.21302035716122
43	18.59806733264634	23.229002975225825	35.84890055235034	22.324029139777497
44	17.638327882850763	23.526078491419682	36.02280931954283	22.812784306186725
45	18.353857399281566	22.916503673766826	35.16175974213219	23.567879184819418
46	19.76345725264384	23.59459139263369	35.97463793222923	20.667313422493244
47	17.23227997076187	23.28075089245598	38.68922413889454	20.797744997887612
48	17.080500982428063	21.305276946358756	38.51788600257957	23.096336068633608
49	17.739476619553336	20.68240190807871	40.13369515894322	21.444426313424728
50	17.958427310382444	20.164922735777147	39.058612619338746	22.818037334501668
51	16.76118392081563	20.486139828907753	36.7796924631338	25.972983787142823
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	391.0
1	634.5
2	878.0
3	37420.0
4	73962.0
5	49523.5
6	25085.0
7	23332.5
8	21580.0
9	20717.5
10	19855.0
11	18925.5
12	17996.0
13	16781.5
14	15567.0
15	14285.5
16	13004.0
17	11826.0
18	10648.0
19	10028.0
20	9408.0
21	8605.5
22	7803.0
23	7528.5
24	7254.0
25	7205.5
26	7365.5
27	7574.0
28	8159.0
29	8744.0
30	9162.5
31	9581.0
32	10880.0
33	12179.0
34	13384.5
35	14590.0
36	17078.0
37	19566.0
38	20264.5
39	20963.0
40	24377.0
41	27791.0
42	31373.0
43	34955.0
44	40256.5
45	45558.0
46	52502.0
47	59446.0
48	66159.0
49	72872.0
50	72797.5
51	72723.0
52	62369.5
53	52016.0
54	46098.0
55	40180.0
56	36387.5
57	32595.0
58	31818.0
59	31041.0
60	30761.0
61	30481.0
62	28399.5
63	26318.0
64	22677.5
65	19037.0
66	14904.0
67	10771.0
68	8500.0
69	6229.0
70	5100.5
71	3972.0
72	3191.0
73	2410.0
74	1790.0
75	957.5
76	745.0
77	541.5
78	338.0
79	249.0
80	160.0
81	122.5
82	85.0
83	54.5
84	24.0
85	20.5
86	17.0
87	9.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	894722.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.39387670928013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67769785441487	24.191001372287886
2	10.783347531254668	6.98628860881568
3	4.440796836537062	4.315638756412285
4	2.2687567488478275	2.9397530562209497
5	1.3251979998739367	2.1464150311650463
6	0.8548242087057752	1.6614642014953698
7	0.5675699610105885	1.2870053938607553
8	0.4094876925763307	1.0611915061828208
9	0.32835115967540834	0.9572910285486899
>10	3.5777000813588997	29.223147215624586
>50	0.6751478415325629	13.914250205254449
>100	0.08338198439482662	4.8084668307138845
>500	0.005629163503448164	1.2704126416052575
>1k	0.0017591135948275512	1.3043754089147008
>5k	0.0	0.0
>10k+	3.5182271896551024E-4	3.9332987428976987
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34512	3.8572875150046606	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3587	0.40090665033384665	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3452	0.38581816474837993	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	2046	0.22867438153974085	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1296	0.144849461620481	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC	1064	0.11891961972545662	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTG	965	0.10785473029611431	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	952	0.1064017650175138	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	917	0.1024899354212817	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2427569680861765	0.0
2	0.0	0.0	0.0	1.3464517470230977	0.0
3	0.0	0.0	0.0	1.9105375747997702	0.0
4	0.0	0.0	0.0	2.478535232172675	0.0
5	0.0	0.0	0.0	3.911382529992556	0.0
6	0.0	0.0	0.0	4.576840627591587	0.0
7	0.0	0.0	0.0	5.355853550041242	0.0
8	0.0	0.0	0.0	6.4958724609431755	0.0
9	0.0	0.0	0.0	6.889961351123589	0.0
10	0.0	0.0	0.0	7.827012189261022	0.0
11	0.0	0.0	0.0	9.291712956650223	0.0
12	0.0	0.0	0.0	10.367577862173949	0.0
13	0.0	0.0	0.0	10.79542025344185	0.0
14	0.0	0.0	0.0	10.97402321614982	0.0
15	0.0	0.0	0.0	11.342294030995102	0.0
16	0.0	0.0	0.0	12.0432938946399	0.0
17	0.0	0.0	0.0	12.875731232718095	0.0
18	0.0	0.0	0.0	13.90577184868596	0.0
19	0.0	0.0	0.0	14.483604963329391	0.0
20	0.0	0.0	0.0	15.106815301289116	0.0
21	0.0	0.0	0.0	15.830056710352489	0.0
22	0.0	0.0	0.0	16.588169286102275	0.0
23	0.0	0.0	0.0	17.318340221878973	0.0
24	0.0	0.0	0.0	17.914055986105183	0.0
25	0.0	0.0	0.0	18.484288974675934	0.0
26	0.0	0.0	0.0	18.95996745357776	0.0
27	0.0	0.0	0.0	19.400327699553603	0.0
28	0.0	0.0	0.0	19.85644703047427	0.0
29	0.0	0.0	0.0	20.329554878498573	0.0
30	0.0	0.0	0.0	20.860669571107003	0.0
31	0.0	0.0	0.0	21.350430636555267	0.0
32	0.0	0.0	0.0	21.832032743131386	0.0
33	0.0	0.0	0.0	22.31911141114223	0.0
34	0.0	0.0	0.0	22.781713202536654	0.0
35	1.1176655989234645E-4	0.0	0.0	23.285221554851674	0.0
36	1.1176655989234645E-4	0.0	0.0	23.805383124590655	0.0
37	1.1176655989234645E-4	0.0	0.0	24.30810911098643	0.0
38	1.1176655989234645E-4	0.0	0.0	24.798540775794045	0.0
39	1.1176655989234645E-4	0.0	0.0	25.312555184738947	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	130	0.0	45.000004	6
GCATAAG	25	3.89011E-5	45.0	1
CTATGCG	20	7.032449E-4	45.0	1
ACGCCCC	20	7.032449E-4	45.0	40
GGTCGAT	25	3.89011E-5	45.0	19
CGTGCAC	20	7.032449E-4	45.0	20
GAGTACG	20	7.032449E-4	45.0	1
ATTAACG	20	7.032449E-4	45.0	34
CGTCTAA	25	3.89011E-5	45.0	43
CGCGATT	20	7.032449E-4	45.0	43
CGATTCG	40	6.8121153E-9	45.0	10
ACGGGTC	55	1.8189894E-12	45.0	5
CTGCGTA	20	7.032449E-4	45.0	16
TAGCACG	20	7.032449E-4	45.0	1
CCGTATC	20	7.032449E-4	45.0	32
CCGTATA	25	3.89011E-5	45.0	30
CTAACGG	25	3.89011E-5	45.0	2
TACGAAT	55	1.8189894E-12	45.0	12
TATTCGA	20	7.032449E-4	45.0	14
AACGCTA	20	7.032449E-4	45.0	27
>>END_MODULE