##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934958.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1554523
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5875461475964	34.0	31.0	34.0	31.0	34.0
2	32.74876923660827	34.0	31.0	34.0	31.0	34.0
3	32.80618041675807	34.0	31.0	34.0	31.0	34.0
4	36.200951674565125	37.0	37.0	37.0	35.0	37.0
5	36.09624238431982	37.0	35.0	37.0	35.0	37.0
6	36.08731360037773	37.0	36.0	37.0	35.0	37.0
7	36.26207396095137	37.0	37.0	37.0	35.0	37.0
8	36.27287470175739	37.0	37.0	37.0	35.0	37.0
9	38.08429016489303	39.0	39.0	39.0	37.0	39.0
10	37.601743428691634	39.0	38.0	39.0	35.0	39.0
11	37.44615808193253	39.0	37.0	39.0	34.0	39.0
12	37.42288341825756	39.0	37.0	39.0	35.0	39.0
13	37.42146690656877	39.0	37.0	39.0	35.0	39.0
14	38.71870342220733	40.0	38.0	41.0	35.0	41.0
15	38.7644943175495	40.0	38.0	41.0	35.0	41.0
16	38.85969007856429	40.0	38.0	41.0	35.0	41.0
17	38.86958700514563	40.0	38.0	41.0	35.0	41.0
18	38.37235859488731	39.0	38.0	41.0	35.0	41.0
19	37.9022709860195	38.0	37.0	41.0	35.0	41.0
20	37.357850607549715	38.0	35.0	41.0	34.0	41.0
21	37.3159400021743	38.0	35.0	41.0	34.0	41.0
22	37.137407423370384	38.0	35.0	41.0	34.0	41.0
23	37.025358904306984	38.0	35.0	40.0	34.0	41.0
24	36.819698389795455	38.0	35.0	40.0	33.0	41.0
25	36.68289436695372	38.0	35.0	40.0	33.0	41.0
26	36.82806815981494	38.0	35.0	40.0	33.0	41.0
27	36.87583522405265	38.0	35.0	40.0	33.0	41.0
28	36.83341835405459	38.0	35.0	40.0	33.0	41.0
29	36.69820259976855	38.0	35.0	40.0	33.0	41.0
30	36.397064565786415	38.0	35.0	40.0	33.0	41.0
31	35.86823996814457	38.0	35.0	40.0	31.0	41.0
32	35.160085762642304	38.0	35.0	41.0	23.0	41.0
33	34.27191620838032	38.0	35.0	41.0	17.0	41.0
34	33.50888343240981	38.0	34.0	41.0	12.0	41.0
35	33.049404222388475	38.0	33.0	41.0	10.0	41.0
36	32.85160914312622	38.0	33.0	41.0	9.0	41.0
37	32.65318750510607	38.0	33.0	41.0	8.0	41.0
38	32.52568279787433	38.0	32.0	41.0	8.0	41.0
39	32.4401575274216	38.0	32.0	41.0	8.0	41.0
40	32.298365479314235	38.0	31.0	40.0	8.0	41.0
41	32.17823023525545	38.0	31.0	40.0	8.0	41.0
42	32.07167471951203	38.0	31.0	40.0	7.0	41.0
43	31.987787250494204	37.0	30.0	40.0	7.0	41.0
44	31.88585244476923	37.0	30.0	40.0	7.0	41.0
45	31.82701896337333	37.0	30.0	40.0	7.0	41.0
46	31.766570838771766	37.0	29.0	40.0	7.0	41.0
47	31.73814861536304	37.0	29.0	40.0	7.0	41.0
48	31.715398228266807	37.0	29.0	40.0	7.0	41.0
49	31.628300128077875	36.0	29.0	40.0	7.0	41.0
50	31.549622617355936	36.0	29.0	40.0	7.0	41.0
51	30.99475594764439	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	12.0
11	12.0
12	28.0
13	21.0
14	34.0
15	63.0
16	115.0
17	266.0
18	458.0
19	905.0
20	1601.0
21	2510.0
22	4273.0
23	7805.0
24	16262.0
25	35078.0
26	61264.0
27	74823.0
28	68130.0
29	53038.0
30	41191.0
31	36401.0
32	36321.0
33	44215.0
34	78631.0
35	99463.0
36	108943.0
37	138457.0
38	271016.0
39	372933.0
40	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.55625423361378	15.679214781640413	18.331218000634276	35.433312984111524
2	50.112735546531	17.63035992391235	18.502202926556894	13.754701602999763
3	23.519883591300996	18.017938621686525	44.966848351552215	13.495329435460265
4	20.90403294129453	19.425508660856096	45.533324370240905	14.13713402760847
5	19.9007669876869	21.480672849485018	44.020448716422976	14.598111446405104
6	20.478243165266775	24.89252330135997	42.8862744391688	11.74295909420446
7	61.21717079773023	3.613198389473813	31.504069093863517	3.6655617189324317
8	62.15205564665174	3.3372938193902564	30.693852712375435	3.816797821582569
9	58.289520322311084	4.621417631003208	32.22750644409893	4.861555602586774
10	31.117841292795283	20.3798850193918	37.70294810691125	10.799325580901666
11	21.782180128566768	19.294343023551274	43.836726764415836	15.08675008346612
12	19.98188511845756	17.0885860164179	47.27430858211812	15.655220283006427
13	18.045728496780043	18.146595450823177	48.59561421735156	15.21206183504522
14	15.307267888606344	20.43018983958423	47.4971422101828	16.76540006162662
15	14.385634693085917	19.79848480852326	50.244480139566924	15.571400358823897
16	16.853079690683252	19.88680772172557	46.93201708820005	16.328095499391132
17	16.75472154480828	19.690670385706742	45.68919211873996	17.86541595074502
18	16.68736969475524	19.259026723953266	48.01028997319435	16.043313608097147
19	16.647679062966585	20.515553645716402	45.95235966273899	16.88440762857803
20	17.891790600718032	20.771645064112914	46.28718906056713	15.04937527460192
21	17.673652946916835	20.164963786319017	46.75974559398606	15.401637672778081
22	17.249921680155264	18.62230407655596	46.3467571724574	17.781017070831375
23	16.293744126011646	19.257933140905603	46.865115537048986	17.58320719603377
24	17.03770224049435	18.556560436867127	46.91702856760563	17.488708755032896
25	15.485714910618883	20.626198518773926	45.41785486609076	18.470231704516436
26	15.28166517960815	20.63906420168759	46.41912663884677	17.660143979857487
27	15.362268683062263	20.325849151154404	46.299668772993385	18.012213392789942
28	13.594266537066355	19.682757990714837	47.447416345721486	19.275559126497324
29	14.883600950259341	18.912746868331958	47.68626774901368	18.51738443239502
30	16.5966666302139	19.699161736429758	45.6570279114558	18.047143721900543
31	16.298375771860567	21.32332554745089	43.325187211768494	19.053111468920047
32	17.084211684227252	21.38167141946436	43.1082717978441	18.42584509846429
33	17.98230068001567	21.8556431780038	40.23916017968212	19.922895962298405
34	16.678749687203084	23.394507511307328	39.756246771517695	20.170496029971897
35	16.820079214009702	23.94972605744656	37.69362048679884	21.536574241744898
36	18.14839664643109	25.13465545379515	36.85419900509674	19.862748894677015
37	17.61234796783322	25.552725820074713	36.750565929227164	20.084360282864903
38	17.550271047774785	25.845548763189736	35.46933689626979	21.13484329276569
39	18.98846141227888	24.95498619190581	36.330244068437715	19.726308327377595
40	19.235739837879528	24.952477383737648	35.639678538046724	20.172104240336104
41	16.95156649338736	25.706663716136717	34.76654896711081	22.575220823365107
42	17.22772837712919	24.533635076483268	35.689983358239154	22.54865318814839
43	18.167180543485042	24.16985789209938	35.867980081349714	21.794981483065868
44	17.99394412305254	24.22048435436465	35.33315364262864	22.452417879954172
45	18.415938522620763	24.16895729429542	35.02264038550732	22.392463797576493
46	19.2772316652761	23.941427691967245	35.695901572379434	21.085439070377216
47	16.230637951320116	24.21508076754091	38.187855695927304	21.366425585211672
48	17.04657956170478	22.25782442588498	38.360899131116106	22.334696881294132
49	17.673202648014858	21.46150298194366	39.6749999839179	21.19029438612359
50	17.385590306479866	21.717787385583872	38.37749586207473	22.519126445861527
51	16.543788673438733	22.13296297320786	36.59321862719304	24.73002972616037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434.0
1	765.5
2	1097.0
3	59041.5
4	116986.0
5	79652.5
6	42319.0
7	40803.5
8	39288.0
9	38585.0
10	37882.0
11	36406.0
12	34930.0
13	32827.5
14	30725.0
15	28169.0
16	25613.0
17	23627.0
18	21641.0
19	19397.0
20	17153.0
21	15624.0
22	14095.0
23	13408.5
24	12722.0
25	12551.0
26	13410.5
27	14441.0
28	15203.0
29	15965.0
30	17344.0
31	18723.0
32	20593.0
33	22463.0
34	26053.5
35	29644.0
36	32916.5
37	36189.0
38	39032.0
39	41875.0
40	46241.0
41	50607.0
42	58641.0
43	66675.0
44	74087.0
45	81499.0
46	95282.5
47	109066.0
48	117920.0
49	126774.0
50	125136.0
51	123498.0
52	109953.0
53	96408.0
54	84199.0
55	71990.0
56	64355.0
57	56720.0
58	52229.0
59	47738.0
60	43915.5
61	40093.0
62	36627.0
63	33161.0
64	28325.0
65	23489.0
66	19555.5
67	15622.0
68	12562.5
69	9503.0
70	8002.5
71	6502.0
72	5366.0
73	4230.0
74	3168.0
75	1508.0
76	910.0
77	835.5
78	761.0
79	553.0
80	345.0
81	244.0
82	143.0
83	105.0
84	67.0
85	41.5
86	16.0
87	15.0
88	14.0
89	13.0
90	12.0
91	8.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1554523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.53901823216474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.54870975925985	24.908198625052435
2	10.138476832076535	6.597921649706365
3	3.949829508963496	3.855707232183166
4	1.9996277010787962	2.6026368889177847
5	1.152690120117552	1.8753702417270597
6	0.7196831213089452	1.40506693233918
7	0.5313185317253291	1.210200837362824
8	0.37608601331639996	0.9789975715331594
9	0.31635546113862006	0.9264506506050998
>10	3.33650337366252	27.21003555658476
>50	0.8445733270088254	17.653901658298025
>100	0.07969032608540434	4.450452697097648
>500	0.003429709761959265	0.7488318966701081
>1k	0.0028244668627899834	1.6708397855744361
>5k	0.0	0.0
>10k+	2.0174763305642738E-4	3.9053877763479794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59491	3.8269617110843646	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	4571	0.29404518299182447	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	3953	0.2542902227885982	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	2003	0.12884981438035975	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	1736	0.11167412769061634	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1716	0.1103875593992498	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	1694	0.10897233427874661	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1366978809576957	0.0
2	0.0	0.0	0.0	0.9162939371112554	0.0
3	0.0	0.0	0.0	1.3157733915805685	0.0
4	0.0	0.0	0.0	1.78414857805256	0.0
5	0.0	0.0	0.0	2.9787272365864	0.0
6	0.0	0.0	0.0	3.530472048338944	0.0
7	0.0	0.0	0.0	4.149890352217368	0.0
8	0.0	0.0	0.0	5.030867989730612	0.0
9	0.0	0.0	0.0	5.341059604779087	0.0
10	0.0	0.0	0.0	6.174562872340904	0.0
11	0.0	0.0	0.0	7.3684339183144925	0.0
12	0.0	0.0	0.0	8.399811389088486	0.0
13	0.0	0.0	0.0	8.759407226525436	0.0
14	0.0	0.0	0.0	8.896941376872519	0.0
15	0.0	0.0	0.0	9.187126854990245	0.0
16	0.0	0.0	0.0	9.799469033266153	0.0
17	0.0	0.0	0.0	10.550245959693102	0.0
18	0.0	0.0	0.0	11.350555765337663	0.0
19	0.0	0.0	0.0	11.886733100764673	0.0
20	0.0	0.0	0.0	12.382383535013634	0.0
21	0.0	0.0	0.0	13.010100204371373	0.0
22	0.0	0.0	0.0	13.677185863444928	0.0
23	0.0	0.0	0.0	14.35366347104546	0.0
24	0.0	0.0	0.0	14.878519005508442	0.0
25	0.0	0.0	0.0	15.362333011476833	0.0
26	0.0	0.0	0.0	15.79082458091646	0.0
27	0.0	0.0	0.0	16.24002989984709	0.0
28	0.0	0.0	0.0	16.67347475720848	0.0
29	0.0	0.0	0.0	17.15046995123263	0.0
30	0.0	0.0	0.0	17.666062193997774	0.0
31	0.0	0.0	0.0	18.133987081567785	0.0
32	0.0	0.0	0.0	18.57380045197144	0.0
33	0.0	0.0	0.0	19.000426497388588	0.0
34	6.432841456832739E-5	0.0	0.0	19.450532414123174	0.0
35	1.2865682913665478E-4	0.0	0.0	19.92579074095398	0.0
36	1.2865682913665478E-4	0.0	0.0	20.384580993655288	0.0
37	1.2865682913665478E-4	0.0	0.0	20.820213017112003	0.0
38	1.2865682913665478E-4	0.0	0.0	21.255394741666738	0.0
39	1.2865682913665478E-4	0.0	0.0	21.716114846805098	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	20	7.0340553E-4	45.0	1
TCCGCGC	20	7.0340553E-4	45.0	29
ATCGTAC	20	7.0340553E-4	45.0	39
GCTCGAT	20	7.0340553E-4	45.0	40
GTACGTC	20	7.0340553E-4	45.0	9
CGTTTTT	40585	0.0	44.595295	1
CGGTCTA	55	6.184564E-11	40.909092	31
TAACGGA	50	1.0822987E-9	40.5	20
TTAACGG	95	0.0	40.263157	2
GTTTTTT	45850	0.0	39.597057	2
CTACGTC	40	3.459263E-7	39.375	30
ACTCTCG	35	6.2497093E-6	38.57143	11
CGAATAT	105	0.0	38.57143	14
TATAGCG	70	0.0	38.57143	1
ATCTCGT	30	1.1401072E-4	37.499996	41
TAACGCG	60	1.5643309E-10	37.499996	1
ATCGACG	30	1.1401072E-4	37.499996	1
ACGGGAG	510	0.0	36.61765	5
CGGTTAG	25	0.0021073367	36.0	1
CGTTGCG	25	0.0021073367	36.0	2
>>END_MODULE