SRR2934956.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934956.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415691
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15432	3.712372892364761	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	2315	0.5569040465153202	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	1722	0.41425000781830734	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	1306	0.31417567375767097	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	941	0.22637006815158378	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC	728	0.17513008460611368	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG	687	0.16526698918186825	No Hit
ACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	596	0.14337572860610404	TruSeq Adapter, Index 14 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACCA	35	1.2094279E-7	45.000004	43
ACCGATT	35	1.2094279E-7	45.000004	10
CCTACAC	35	1.2094279E-7	45.000004	28
CGACAGG	35	1.2094279E-7	45.000004	2
ACGATTG	35	1.2094279E-7	45.000004	1
CGAGAGA	35	1.2094279E-7	45.000004	32
CGATAGC	35	1.2094279E-7	45.000004	13
TGGAGTA	20	7.0280855E-4	45.0	33
AGGTACG	20	7.0280855E-4	45.0	1
TCTTGCG	25	3.8864935E-5	45.0	1
GTCGATT	20	7.0280855E-4	45.0	9
ATGATCG	25	3.8864935E-5	45.0	27
TTGTGCG	75	0.0	45.0	1
GTACACG	20	7.0280855E-4	45.0	1
CAAGTAG	20	7.0280855E-4	45.0	1
GGTCGAT	20	7.0280855E-4	45.0	8
GCGGTTG	20	7.0280855E-4	45.0	5
GCGGTAT	25	3.8864935E-5	45.0	21
TAGACGA	20	7.0280855E-4	45.0	14
TGGACCG	20	7.0280855E-4	45.0	18