##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934956.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415691 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.512604314262276 34.0 31.0 34.0 31.0 34.0 2 32.68725327226233 34.0 31.0 34.0 31.0 34.0 3 32.73913074856083 34.0 31.0 34.0 31.0 34.0 4 36.16578901154944 37.0 37.0 37.0 35.0 37.0 5 36.04048199263395 37.0 35.0 37.0 35.0 37.0 6 36.04561080225456 37.0 35.0 37.0 35.0 37.0 7 36.231573452396134 37.0 37.0 37.0 35.0 37.0 8 36.24663993206492 37.0 37.0 37.0 35.0 37.0 9 38.05498795980668 39.0 39.0 39.0 35.0 39.0 10 37.56689223485714 39.0 38.0 39.0 35.0 39.0 11 37.415010187855884 39.0 37.0 39.0 34.0 39.0 12 37.298197459170396 39.0 37.0 39.0 35.0 39.0 13 37.177425058517024 39.0 37.0 39.0 33.0 39.0 14 38.35461677063011 40.0 38.0 41.0 33.0 41.0 15 38.45937487220075 40.0 38.0 41.0 34.0 41.0 16 38.604287800313216 40.0 38.0 41.0 35.0 41.0 17 38.63647757589171 40.0 38.0 41.0 35.0 41.0 18 38.17043669456399 39.0 37.0 40.0 35.0 41.0 19 37.711196537812945 38.0 37.0 40.0 35.0 41.0 20 37.14753266248247 38.0 35.0 40.0 34.0 41.0 21 37.07699950203396 38.0 35.0 40.0 34.0 41.0 22 36.87825812923542 37.0 35.0 40.0 34.0 41.0 23 36.75751459617841 37.0 35.0 40.0 33.0 41.0 24 36.57724367378654 37.0 35.0 40.0 33.0 41.0 25 36.38963557065224 36.0 35.0 40.0 33.0 41.0 26 36.523153015100156 37.0 35.0 40.0 33.0 41.0 27 36.56228063633805 37.0 35.0 40.0 33.0 41.0 28 36.54171006829592 37.0 35.0 40.0 33.0 41.0 29 36.44461150229377 37.0 35.0 40.0 33.0 41.0 30 36.159334698129136 37.0 35.0 40.0 32.0 41.0 31 35.60476892691928 37.0 35.0 40.0 30.0 41.0 32 34.81065502981782 37.0 35.0 40.0 23.0 41.0 33 33.94542099780847 37.0 34.0 40.0 18.0 41.0 34 33.2423434714728 37.0 33.0 40.0 12.0 41.0 35 32.70102552136082 37.0 33.0 40.0 10.0 41.0 36 32.546723407531076 37.0 33.0 40.0 10.0 41.0 37 32.37816310673072 37.0 32.0 40.0 8.0 41.0 38 32.246724129220986 36.0 31.0 40.0 8.0 41.0 39 32.24842731740644 37.0 31.0 40.0 8.0 41.0 40 32.085878693548814 37.0 31.0 40.0 8.0 41.0 41 31.87211895374208 36.0 31.0 40.0 7.0 41.0 42 31.683430721377178 36.0 30.0 40.0 7.0 41.0 43 31.605401608406243 36.0 30.0 40.0 7.0 41.0 44 31.51096607816864 36.0 29.0 40.0 7.0 41.0 45 31.42970860567104 36.0 29.0 40.0 7.0 41.0 46 31.346358232437076 35.0 28.0 40.0 7.0 41.0 47 31.2720169549016 35.0 28.0 40.0 7.0 41.0 48 31.20568162409097 35.0 27.0 40.0 7.0 41.0 49 31.137414569957013 35.0 27.0 40.0 7.0 41.0 50 31.056154691826382 35.0 27.0 40.0 7.0 41.0 51 30.489062789427724 35.0 25.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 1.0 11 4.0 12 4.0 13 7.0 14 8.0 15 17.0 16 24.0 17 75.0 18 148.0 19 237.0 20 485.0 21 721.0 22 1167.0 23 2300.0 24 4447.0 25 9745.0 26 16969.0 27 20700.0 28 17946.0 29 13760.0 30 11145.0 31 10053.0 32 10264.0 33 13421.0 34 25510.0 35 36583.0 36 30195.0 37 37773.0 38 69997.0 39 81930.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.028773776675465 16.287338431671603 18.78029594097539 35.90359185067754 2 49.15405914489368 17.697039387429605 19.296544789278574 13.852356678398136 3 23.01805908715849 18.348725375338894 46.42823635825649 12.204979179246124 4 22.003122511673332 19.687941283308994 44.73563295813477 13.573303246882901 5 18.28978736609645 21.75630456276417 45.099124109013665 14.854783962125714 6 20.254708425248563 24.51388170540137 43.11255235258882 12.11885751676125 7 60.35155921104859 3.8961632558799684 31.545547052979256 4.206730480092183 8 60.40544539092739 2.9240469483342193 31.796214014736908 4.874293646001477 9 56.81912766935056 4.773738185334779 33.26773973937372 5.139394405940951 10 30.620100026221397 21.155136868491258 38.31932853970858 9.905434565578759 11 23.1583074928252 16.891392885580874 45.63052844540776 14.319771176186158 12 21.82654904724904 15.82473519994419 48.44319458443892 13.905521168367851 13 16.450680914429228 21.738984004945983 48.47518950374196 13.335145576882828 14 12.360383073003746 24.472264253977112 47.358254087771925 15.809098585247213 15 11.047388565063955 20.512832849400155 54.51501235292561 13.924766232610281 16 13.319508962185855 19.291492959914937 51.40982123740952 15.97917684048969 17 13.640660971731405 18.248651041278258 46.69261542828688 21.41807255870346 18 14.739794703277193 18.572930373763207 49.74584487034841 16.941430052611196 19 16.844723604792982 18.726650324399614 47.12081810768095 17.307807963126457 20 17.850278211459955 20.894847374612393 46.42029776925649 14.834576644671163 21 16.01117175979273 20.916257508582095 47.73810354325689 15.334467188368283 22 14.829043688701463 19.25997916721796 45.80638021992297 20.104596924157608 23 13.116232971125186 20.858762879157837 47.08353079571124 18.94147335400574 24 16.24018802427765 18.426667885520736 48.4128836082571 16.92026048194452 25 13.178057740003993 21.41302072933982 47.11961529116579 18.28930623949039 26 12.511937953912883 23.803738834855697 46.38974622977163 17.294576981459787 27 13.964459177610292 24.06258494891638 46.14148490104428 15.83147097242904 28 12.543451746609863 21.517665766158036 48.554815957045015 17.384066530187088 29 13.42631906873134 20.7452170001275 46.828293131196006 19.000170799945153 30 16.70134787618688 21.90425099412785 44.55015865149835 16.84424247818692 31 19.679281004399904 21.938892109764225 41.2647856220125 17.117041263823367 32 17.96165902076302 23.18452889285551 41.01171302722455 17.842099059156922 33 18.523614896641977 23.15951030934035 40.199811879497034 18.11706291452064 34 17.797835411399333 23.041153164249405 38.934930032163315 20.226081392187947 35 16.404492760247397 24.777057958916597 37.276967747677965 21.541481533158045 36 21.087538580339725 25.336848765068282 36.91107096376876 16.66454169082323 37 18.96119954485423 26.277691843220087 37.86755065661754 16.89355795530815 38 18.00207365567212 25.55744531394714 36.22113541067764 20.2193456197031 39 19.476486139945294 24.33730824097707 37.39652770928406 18.78967790979357 40 21.014888462824548 24.021208060795157 36.58462656155654 18.37927691482375 41 17.755255706762956 24.72461515885598 35.615156450344124 21.90497268403694 42 18.054757019035776 25.023875907825765 36.497302082556516 20.424064990581947 43 19.764440413672656 24.973117050886355 34.830679519162075 20.43176301627892 44 18.79376748594509 24.973838740795447 33.930972765828464 22.301421007431 45 18.476464489248023 25.4383664789471 32.50635688528258 23.578812146522296 46 21.024751558248795 24.938957061856044 34.76115672458629 19.27513465530887 47 17.408844550399216 23.963232304764837 38.518274391314705 20.109648753521245 48 16.992429472853633 22.321628324885552 38.23537194695098 22.450570255309835 49 18.284735536732814 20.526785520975917 40.211118354739455 20.97736058755181 50 18.03382801167213 21.676918672764145 38.057114539405475 22.23213877615825 51 16.55965609070199 22.30166157073403 34.86147162194996 26.277210716614025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 364.0 1 438.0 2 512.0 3 13791.5 4 27071.0 5 18554.5 6 10038.0 7 10043.0 8 10048.0 9 10428.0 10 10808.0 11 10676.5 12 10545.0 13 9913.0 14 9281.0 15 8505.5 16 7730.0 17 7042.5 18 6355.0 19 5780.0 20 5205.0 21 4647.5 22 4090.0 23 3818.0 24 3546.0 25 3547.0 26 3844.0 27 4140.0 28 4289.0 29 4438.0 30 5001.0 31 5564.0 32 6078.5 33 6593.0 34 7173.0 35 7753.0 36 8366.5 37 8980.0 38 9350.0 39 9720.0 40 11575.0 41 13430.0 42 17436.5 43 21443.0 44 24655.5 45 27868.0 46 30918.0 47 33968.0 48 37696.5 49 41425.0 50 39882.0 51 38339.0 52 31613.0 53 24887.0 54 20976.0 55 17065.0 56 14462.0 57 11859.0 58 9948.5 59 8038.0 60 7568.0 61 7098.0 62 6172.0 63 5246.0 64 4589.0 65 3932.0 66 2872.5 67 1813.0 68 1528.5 69 1244.0 70 983.0 71 722.0 72 616.5 73 511.0 74 430.0 75 203.0 76 57.0 77 45.0 78 33.0 79 31.0 80 29.0 81 15.0 82 1.0 83 1.5 84 2.0 85 1.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 415691.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.202420768831686 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95183743501623 28.62794635192208 2 10.096245103141118 7.512095170246249 3 4.09960921561205 4.575461610809387 4 2.0517512801755147 3.053204577523143 5 1.1947433508388587 2.222367242433556 6 0.778893159626827 1.7386026635041205 7 0.5321743530248074 1.3858721942526764 8 0.35625209523765045 1.0602752277447178 9 0.27176908531799093 0.9099421077564377 >10 3.1195011250759745 28.302071685472203 >50 0.48975876813809915 11.483349200504993 >100 0.05224093526806391 3.3646657730646754 >500 0.0026120467634031955 0.7171475351687309 >1k 0.0019590350725523964 1.298007886316575 >5k 0.0 0.0 >10k+ 6.530116908507989E-4 3.748990773280439 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15432 3.712372892364761 No Hit CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC 2315 0.5569040465153202 TruSeq Adapter, Index 21 (95% over 23bp) GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC 1722 0.41425000781830734 TruSeq Adapter, Index 21 (95% over 23bp) CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT 1306 0.31417567375767097 TruSeq Adapter, Index 14 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC 941 0.22637006815158378 TruSeq Adapter, Index 21 (95% over 23bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC 728 0.17513008460611368 No Hit CGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG 687 0.16526698918186825 No Hit ACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC 596 0.14337572860610404 TruSeq Adapter, Index 14 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3415998903031338 0.0 2 0.0 0.0 0.0 1.7849797084853893 0.0 3 0.0 0.0 0.0 2.4910330028795427 0.0 4 0.0 0.0 0.0 3.226435020243402 0.0 5 0.0 0.0 0.0 5.102107093971243 0.0 6 0.0 0.0 0.0 5.960677522486654 0.0 7 0.0 0.0 0.0 6.9493926979415 0.0 8 0.0 0.0 0.0 8.411055327154063 0.0 9 0.0 0.0 0.0 8.944384169972407 0.0 10 0.0 0.0 0.0 10.069498738245475 0.0 11 0.0 0.0 0.0 11.846780421033893 0.0 12 0.0 0.0 0.0 13.257924756610079 0.0 13 0.0 0.0 0.0 13.767678395731444 0.0 14 0.0 0.0 0.0 13.95652058861029 0.0 15 0.0 0.0 0.0 14.356336798246774 0.0 16 0.0 0.0 0.0 15.221161872640977 0.0 17 0.0 0.0 0.0 16.31716828124737 0.0 18 0.0 0.0 0.0 17.718449521399307 0.0 19 0.0 0.0 0.0 18.451927032338926 0.0 20 0.0 0.0 0.0 19.102650767036092 0.0 21 0.0 0.0 0.0 19.899877553278756 0.0 22 0.0 0.0 0.0 20.686519554188088 0.0 23 0.0 0.0 0.0 21.5070809808247 0.0 24 0.0 0.0 0.0 22.10824867509761 0.0 25 0.0 0.0 0.0 22.64759160049171 0.0 26 0.0 0.0 0.0 23.138821865279738 0.0 27 0.0 0.0 0.0 23.573038627249566 0.0 28 0.0 0.0 0.0 24.081589449855784 0.0 29 0.0 0.0 0.0 24.607941956886243 0.0 30 0.0 0.0 0.0 25.23220372825007 0.0 31 0.0 0.0 0.0 25.805947205977517 0.0 32 0.0 0.0 0.0 26.28899831846251 0.0 33 0.0 0.0 0.0 26.734762118977798 0.0 34 0.0 0.0 0.0 27.19111070482642 0.0 35 0.0 0.0 0.0 27.697737021008393 0.0 36 0.0 0.0 0.0 28.151198847220652 0.0 37 0.0 0.0 0.0 28.59311363488745 0.0 38 0.0 0.0 0.0 29.057641373039107 0.0 39 0.0 0.0 0.0 29.58399388006957 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCACCA 35 1.2094279E-7 45.000004 43 ACCGATT 35 1.2094279E-7 45.000004 10 CCTACAC 35 1.2094279E-7 45.000004 28 CGACAGG 35 1.2094279E-7 45.000004 2 ACGATTG 35 1.2094279E-7 45.000004 1 CGAGAGA 35 1.2094279E-7 45.000004 32 CGATAGC 35 1.2094279E-7 45.000004 13 TGGAGTA 20 7.0280855E-4 45.0 33 AGGTACG 20 7.0280855E-4 45.0 1 TCTTGCG 25 3.8864935E-5 45.0 1 GTCGATT 20 7.0280855E-4 45.0 9 ATGATCG 25 3.8864935E-5 45.0 27 TTGTGCG 75 0.0 45.0 1 GTACACG 20 7.0280855E-4 45.0 1 CAAGTAG 20 7.0280855E-4 45.0 1 GGTCGAT 20 7.0280855E-4 45.0 8 GCGGTTG 20 7.0280855E-4 45.0 5 GCGGTAT 25 3.8864935E-5 45.0 21 TAGACGA 20 7.0280855E-4 45.0 14 TGGACCG 20 7.0280855E-4 45.0 18 >>END_MODULE