##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934953.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434273
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.394263516267415	34.0	31.0	34.0	30.0	34.0
2	32.57869358675303	34.0	31.0	34.0	31.0	34.0
3	32.64158029626525	34.0	31.0	34.0	31.0	34.0
4	36.08321493622675	37.0	35.0	37.0	35.0	37.0
5	35.95141535393635	37.0	35.0	37.0	35.0	37.0
6	35.943160178044685	37.0	35.0	37.0	35.0	37.0
7	36.13432794578525	37.0	36.0	37.0	35.0	37.0
8	36.138700771173895	37.0	37.0	37.0	35.0	37.0
9	37.93146016445876	39.0	39.0	39.0	35.0	39.0
10	37.417569132780535	39.0	37.0	39.0	34.0	39.0
11	37.31403978603321	39.0	37.0	39.0	34.0	39.0
12	37.199328532973496	39.0	37.0	39.0	34.0	39.0
13	37.06889675388523	39.0	37.0	39.0	33.0	39.0
14	38.22980935955033	40.0	37.0	41.0	33.0	41.0
15	38.329739587770824	40.0	37.0	41.0	33.0	41.0
16	38.442290909174645	40.0	37.0	41.0	34.0	41.0
17	38.48619186548554	40.0	37.0	41.0	34.0	41.0
18	38.050514307820194	39.0	37.0	40.0	34.0	41.0
19	37.600474816532454	38.0	37.0	40.0	34.0	41.0
20	37.06211069995141	38.0	35.0	40.0	34.0	41.0
21	36.98306364890288	38.0	35.0	40.0	34.0	41.0
22	36.77745565577413	37.0	35.0	40.0	33.0	41.0
23	36.66563198725226	37.0	35.0	40.0	33.0	41.0
24	36.44585778991556	37.0	35.0	40.0	33.0	41.0
25	36.31905506444103	37.0	35.0	40.0	33.0	41.0
26	36.487835992566886	37.0	35.0	40.0	33.0	41.0
27	36.564736928153486	37.0	35.0	40.0	33.0	41.0
28	36.547586426049975	37.0	35.0	40.0	33.0	41.0
29	36.45048160949449	37.0	35.0	40.0	33.0	41.0
30	36.14495490164021	37.0	35.0	40.0	32.0	41.0
31	35.581387744575416	37.0	35.0	40.0	30.0	41.0
32	34.89244093001407	37.0	35.0	40.0	24.0	41.0
33	34.005924844510254	37.0	34.0	40.0	18.0	41.0
34	33.320514975602904	37.0	34.0	40.0	12.0	41.0
35	32.91090397054388	37.0	33.0	40.0	10.0	41.0
36	32.73833279987473	37.0	33.0	40.0	10.0	41.0
37	32.53114054983847	37.0	33.0	40.0	9.0	41.0
38	32.394332597237224	37.0	32.0	40.0	9.0	41.0
39	32.33698618150334	37.0	32.0	40.0	8.0	41.0
40	32.184441583980586	37.0	31.0	40.0	8.0	41.0
41	32.0434012706293	36.0	31.0	40.0	8.0	41.0
42	31.919990420772187	36.0	31.0	40.0	7.0	41.0
43	31.82961409067568	36.0	31.0	40.0	7.0	41.0
44	31.70189949639973	36.0	30.0	40.0	7.0	41.0
45	31.65582248953999	36.0	30.0	40.0	7.0	41.0
46	31.612435035104646	36.0	30.0	40.0	7.0	41.0
47	31.547312404869746	36.0	30.0	40.0	7.0	41.0
48	31.52137250070808	35.0	30.0	40.0	7.0	41.0
49	31.440925408671504	36.0	29.0	40.0	7.0	41.0
50	31.353049809681927	35.0	29.0	40.0	7.0	41.0
51	30.731615826910723	35.0	27.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	8.0
10	5.0
11	4.0
12	7.0
13	5.0
14	7.0
15	16.0
16	37.0
17	80.0
18	154.0
19	320.0
20	544.0
21	868.0
22	1403.0
23	2543.0
24	4979.0
25	10216.0
26	17540.0
27	20612.0
28	17810.0
29	13597.0
30	11074.0
31	10226.0
32	10892.0
33	14375.0
34	26754.0
35	38085.0
36	33030.0
37	41766.0
38	75774.0
39	81490.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.06858128412312	16.444724862010762	19.731367135419426	34.755326718446696
2	48.68711616886152	18.76377301835481	18.32188508150403	14.227225731279633
3	22.749284436287773	18.673276947910647	44.53028394581289	14.047154669988693
4	20.8847890612587	20.13203676028673	44.6214708259551	14.361703352499463
5	18.63666403391415	22.296804084066935	43.76302464118193	15.303507240836986
6	19.67195750138738	24.305908955887194	43.2269563154974	12.795177227228033
7	59.05248541815863	4.630497406009583	31.22183511293587	5.095182062895921
8	58.532305715529176	3.7900122733856354	31.576911297732075	6.100770713353121
9	54.55070888588514	4.967612538656559	34.124156924331004	6.3575216511272865
10	30.294998768056036	17.22626090040136	40.42733487921193	12.051405452330677
11	23.560755561593744	19.23582631202032	41.708326329290564	15.495091797095375
12	21.144994047523102	16.859671220637708	46.85048345165369	15.144851280185506
13	16.175078809873053	22.579805790366887	46.81248891826109	14.432626481498964
14	12.443094551123373	26.626338731627342	44.79394298056752	16.136623736681766
15	11.505205251074784	20.850709116155045	52.599862298600186	15.044223334169981
16	11.976337465142894	19.615771645946214	49.92550768756059	18.482383201350302
17	12.249898105569539	19.707188795987776	44.31452105012285	23.728392048319837
18	13.714875205228047	19.48451780331727	49.05002153023559	17.750585461219096
19	15.38962818319352	20.62043921680602	45.751405222060775	18.238527377939683
20	17.68081368171634	20.947883013680336	45.32517563836573	16.0461276662376
21	15.416569761417357	21.423850895634775	46.38994365295563	16.769635689992242
22	14.206271170438873	20.313259171074417	44.05638849295259	21.424081165534123
23	13.201143059780367	20.45003949128774	44.399260373083294	21.949557075848602
24	15.615522954454915	18.784957849094923	47.25714009390406	18.342379102546094
25	13.43739997651247	20.41895305487562	45.049312298945594	21.094334669666313
26	12.021470365415302	22.245453896512103	44.9539805606151	20.7790951774575
27	14.352722826424852	21.69718126616207	46.88640555595213	17.06369035146095
28	11.172004706716743	20.204802048481024	49.59414930239734	19.029043942404893
29	14.572630580303173	18.0216131327529	46.906669307094845	20.499086979849082
30	16.576439244438408	19.643173763968747	45.97937242241631	17.80101456917653
31	16.695719052301204	20.85738694323617	43.87585689186295	18.57103711259968
32	17.51939448227267	21.311018644953748	40.847577445523896	20.32200942724968
33	17.792494582900616	23.756024436241717	39.63819993414281	18.813281046714856
34	15.476209665348756	21.7367876888501	41.14669804477828	21.64030460102286
35	16.308635351495486	21.751985502207134	37.208622226111224	24.73075692018615
36	19.773967066798996	23.30101111512804	37.807784504217395	19.11723731385557
37	17.158561549992747	23.896719344743975	40.40661058827052	18.538108516992768
38	18.429190854600677	26.581666371153627	33.97402094995544	21.01512182429025
39	20.067330918569656	24.686775369410483	36.16918390044972	19.07670981157014
40	19.89670092315203	22.11143681509097	36.63294747773866	21.35891478401835
41	16.01734392881897	23.693621293518135	34.41084294902055	25.87819182864235
42	17.644200767719845	23.60680954146355	34.557985414704575	24.19100427611203
43	19.82715941354862	23.584012821427997	35.17234550616778	21.416482258855606
44	18.091154642356305	23.683259148047426	35.24326863516728	22.982317574428986
45	19.300762423636744	22.975639747347866	33.43472884568002	24.28886898333537
46	20.87788096427823	21.87425881876147	36.250699444819276	20.997160772141026
47	15.638549944389817	22.936263594559183	38.910316782300534	22.51486967875046
48	16.941417034906614	21.294899751999317	37.414253246229904	24.34942996686416
49	18.250501412705834	19.59758032389764	39.97761776578327	22.174300497613253
50	18.335471005565623	19.606100310173556	38.36987332852837	23.688555355732454
51	16.108070269162486	20.463164875550632	35.148397436635484	28.2803674186514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	822.0
1	817.0
2	812.0
3	14260.5
4	27709.0
5	18816.0
6	9923.0
7	9937.5
8	9952.0
9	10273.5
10	10595.0
11	10483.0
12	10371.0
13	9760.5
14	9150.0
15	8319.5
16	7489.0
17	6855.5
18	6222.0
19	5612.5
20	5003.0
21	4502.0
22	4001.0
23	3940.5
24	3880.0
25	3659.5
26	3421.0
27	3403.0
28	3859.5
29	4316.0
30	4203.0
31	4090.0
32	4197.0
33	4304.0
34	5264.0
35	6224.0
36	6374.5
37	6525.0
38	8132.5
39	9740.0
40	11724.0
41	13708.0
42	16921.5
43	20135.0
44	24352.0
45	28569.0
46	33112.5
47	37656.0
48	42958.5
49	48261.0
50	47109.5
51	45958.0
52	36501.5
53	27045.0
54	22848.5
55	18652.0
56	15976.5
57	13301.0
58	10924.0
59	8547.0
60	7967.0
61	7387.0
62	6530.0
63	5673.0
64	4896.0
65	4119.0
66	3472.5
67	2826.0
68	2286.0
69	1746.0
70	1366.5
71	987.0
72	844.5
73	702.0
74	599.5
75	425.0
76	353.0
77	204.5
78	56.0
79	69.5
80	83.0
81	45.0
82	7.0
83	18.5
84	30.0
85	16.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.446705416428244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.74131594839086	28.362627461720113
2	10.33235999510398	7.7382568198629365
3	4.366081016991467	4.904860490026168
4	2.316891159469517	3.4703976292232754
5	1.3754028737138886	2.5752153120436425
6	0.9198499069572489	2.0667209095894217
7	0.5987588726821675	1.5695082984561255
8	0.44018719587853783	1.3186848201717762
9	0.3275913600455179	1.1040495440932432
>10	3.1721543464152173	28.399101859490926
>50	0.3521649628920916	8.252620710620054
>100	0.05164256522975901	3.7136793197790237
>500	0.0018665987432443017	0.5573198401977179
>1k	0.0031109979054071697	2.343213057219251
>5k	0.0	0.0
>10k+	6.221995810814339E-4	3.6237439275063155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15553	3.581387744575417	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	3211	0.7393966468097257	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2680	0.6171233302553923	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	1890	0.435210109769661	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1263	0.2908308828778211	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	1013	0.23326340804056436	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC	899	0.20701263951477528	No Hit
ACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	791	0.18214349038508035	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	702	0.16164946934301694	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	476	0.10960847209013685	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	468	0.10776631289534463	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.30269899349027E-4	0.0	0.0	0.46030952879870496	0.0
2	2.30269899349027E-4	0.0	0.0	2.402636129807748	0.0
3	2.30269899349027E-4	0.0	0.0	3.293780640288482	0.0
4	2.30269899349027E-4	0.0	0.0	4.157292762847334	0.0
5	2.30269899349027E-4	0.0	0.0	6.3280471040106105	0.0
6	2.30269899349027E-4	0.0	0.0	7.371169748061703	0.0
7	2.30269899349027E-4	0.0	0.0	8.561204587897475	0.0
8	2.30269899349027E-4	0.0	0.0	10.23779972505774	0.0
9	2.30269899349027E-4	0.0	0.0	10.878871124845432	0.0
10	2.30269899349027E-4	0.0	0.0	12.24252946879037	0.0
11	2.30269899349027E-4	0.0	0.0	14.337755282967166	0.0
12	2.30269899349027E-4	0.0	0.0	15.904972217936644	0.0
13	2.30269899349027E-4	0.0	0.0	16.51610853080896	0.0
14	2.30269899349027E-4	0.0	0.0	16.756510305729346	0.0
15	2.30269899349027E-4	0.0	0.0	17.20047067167427	0.0
16	2.30269899349027E-4	0.0	0.0	18.275370561835526	0.0
17	2.30269899349027E-4	0.0	0.0	19.59527762490415	0.0
18	4.60539798698054E-4	0.0	0.0	21.30687378676547	0.0
19	4.60539798698054E-4	0.0	0.0	22.135845424421966	0.0
20	4.60539798698054E-4	0.0	0.0	22.843464825121526	0.0
21	4.60539798698054E-4	0.0	0.0	23.75326119744953	0.0
22	4.60539798698054E-4	0.0	0.0	24.685163480115044	0.0
23	4.60539798698054E-4	0.0	0.0	25.621671160767537	0.0
24	4.60539798698054E-4	0.0	0.0	26.381792098518673	0.0
25	4.60539798698054E-4	0.0	0.0	26.95769711679059	0.0
26	4.60539798698054E-4	0.0	0.0	27.526463768182687	0.0
27	4.60539798698054E-4	0.0	0.0	28.019241352789603	0.0
28	4.60539798698054E-4	0.0	0.0	28.53343403803598	0.0
29	4.60539798698054E-4	0.0	0.0	29.11141148540204	0.0
30	4.60539798698054E-4	0.0	0.0	29.779424463413566	0.0
31	4.60539798698054E-4	0.0	0.0	30.359244069974416	0.0
32	4.60539798698054E-4	0.0	0.0	30.89393077626286	0.0
33	4.60539798698054E-4	0.0	0.0	31.402827253824206	0.0
34	4.60539798698054E-4	0.0	0.0	31.93198748252827	0.0
35	4.60539798698054E-4	0.0	0.0	32.52170869476113	0.0
36	4.60539798698054E-4	0.0	0.0	33.02623004423485	0.0
37	4.60539798698054E-4	0.0	0.0	33.52821842481573	0.0
38	4.60539798698054E-4	0.0	0.0	34.01639061143566	0.0
39	4.60539798698054E-4	0.0	0.0	34.49212822349075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTTG	20	7.028432E-4	45.000004	14
GTCATGC	20	7.028432E-4	45.000004	14
AGGTATC	20	7.028432E-4	45.000004	9
AACGTCG	30	2.162371E-6	45.000004	1
ACAGTCC	20	7.028432E-4	45.000004	30
ACTGATC	20	7.028432E-4	45.000004	17
CGTGCTC	20	7.028432E-4	45.000004	28
TAGAATG	20	7.028432E-4	45.000004	2
TTATGCG	20	7.028432E-4	45.000004	1
AGCCAAT	20	7.028432E-4	45.000004	30
ACATTAC	20	7.028432E-4	45.000004	12
TACGGGT	30	2.162371E-6	45.000004	4
CGTTAGG	40	6.7975634E-9	45.000004	2
TACGCCA	20	7.028432E-4	45.000004	36
TCTACGT	20	7.028432E-4	45.000004	24
AGGTTCC	20	7.028432E-4	45.000004	15
GCTTACT	30	2.162371E-6	45.000004	15
GCCCTAT	20	7.028432E-4	45.000004	23
GCTAGTC	40	6.7975634E-9	45.000004	27
GCGATAT	20	7.028432E-4	45.000004	9
>>END_MODULE