SRR2934951.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934951.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	630308
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19854	3.1498886258781424	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	5687	0.9022573091250626	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	5589	0.8867093547916257	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	4666	0.7402730093858876	TruSeq Adapter, Index 21 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	4559	0.7232971816952981	RNA PCR Primer, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	3370	0.5346592459559454	RNA PCR Primer, Index 21 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	1981	0.31429079116876196	RNA PCR Primer, Index 21 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCGATCT	1396	0.22147902295385746	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	1351	0.2143396561680956	TruSeq Adapter, Index 21 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT	1044	0.16563330942967563	RNA PCR Primer, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	958	0.1519891862391085	RNA PCR Primer, Index 21 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	853	0.13533066373899744	RNA PCR Primer, Index 14 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTA	843	0.1337441377866059	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	788	0.1250182450484525	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC	778	0.12343171909606097	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	697	0.11058085888168959	RNA PCR Primer, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	644	0.10217227133401449	TruSeq Adapter, Index 21 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	25	3.888794E-5	45.0	1
TTTAGCG	70	0.0	45.0	1
AACCGTC	20	7.0308597E-4	45.0	13
CGGCGAG	20	7.0308597E-4	45.0	16
TGCCCGC	20	7.0308597E-4	45.0	12
TCCGCAT	20	7.0308597E-4	45.0	34
GGTCCGT	25	3.888794E-5	45.0	33
TACGGAC	25	3.888794E-5	45.0	29
CATTCGC	25	3.888794E-5	45.0	38
TGGCCGT	20	7.0308597E-4	45.0	16
CGTTAAT	20	7.0308597E-4	45.0	38
AAACGAT	20	7.0308597E-4	45.0	29
AATGCGC	25	3.888794E-5	45.0	25
TCGGAGT	20	7.0308597E-4	45.0	43
GAGCGTT	20	7.0308597E-4	45.0	23
CGTAGCA	20	7.0308597E-4	45.0	17
GTCGTAG	35	1.2107557E-7	45.0	1
GGTGCTA	25	3.888794E-5	45.0	36
TGCTCGA	20	7.0308597E-4	45.0	24
CGTGTAG	20	7.0308597E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content