##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934946.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572151
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5441185980624	34.0	31.0	34.0	31.0	34.0
2	32.704249402692646	34.0	31.0	34.0	31.0	34.0
3	32.75628286938238	34.0	31.0	34.0	31.0	34.0
4	36.16135251008912	37.0	37.0	37.0	35.0	37.0
5	36.05690980178309	37.0	35.0	37.0	35.0	37.0
6	36.04078468795825	37.0	35.0	37.0	35.0	37.0
7	36.207990547949755	37.0	37.0	37.0	35.0	37.0
8	36.20700304639859	37.0	37.0	37.0	35.0	37.0
9	38.0065961608037	39.0	39.0	39.0	35.0	39.0
10	37.60030306684774	39.0	38.0	39.0	35.0	39.0
11	37.50666345073241	39.0	37.0	39.0	35.0	39.0
12	37.385990761180175	39.0	37.0	39.0	35.0	39.0
13	37.29762248077868	39.0	37.0	39.0	34.0	39.0
14	38.559693157925096	40.0	38.0	41.0	34.0	41.0
15	38.64356961711157	40.0	38.0	41.0	34.0	41.0
16	38.73253214623412	40.0	38.0	41.0	35.0	41.0
17	38.74351700862185	40.0	38.0	41.0	35.0	41.0
18	38.237891745360926	39.0	38.0	40.0	35.0	41.0
19	37.746505730130686	38.0	37.0	40.0	35.0	41.0
20	37.16244837464236	38.0	35.0	40.0	34.0	41.0
21	37.090589721944035	38.0	35.0	40.0	34.0	41.0
22	36.904076021889324	38.0	35.0	40.0	34.0	41.0
23	36.77190287179433	37.0	35.0	40.0	33.0	41.0
24	36.56995793068613	37.0	35.0	40.0	33.0	41.0
25	36.420081412074786	37.0	35.0	40.0	33.0	41.0
26	36.5555578859427	37.0	35.0	40.0	33.0	41.0
27	36.55718682655453	37.0	35.0	40.0	33.0	41.0
28	36.5088866400653	37.0	35.0	40.0	33.0	41.0
29	36.36811785699929	37.0	35.0	40.0	33.0	41.0
30	36.040688559488665	37.0	35.0	40.0	32.0	41.0
31	35.487392314266685	37.0	35.0	40.0	30.0	41.0
32	34.67204636538256	37.0	35.0	40.0	23.0	41.0
33	33.77253557190322	37.0	34.0	40.0	16.0	41.0
34	33.07370956268537	37.0	33.0	40.0	12.0	41.0
35	32.51878787243228	36.0	33.0	40.0	10.0	41.0
36	32.31695828548757	36.0	32.0	40.0	8.0	41.0
37	32.0900583936758	36.0	31.0	40.0	8.0	41.0
38	31.918240114934694	36.0	31.0	40.0	8.0	41.0
39	31.88917436131371	36.0	31.0	40.0	8.0	41.0
40	31.703772255925447	36.0	30.0	40.0	7.0	41.0
41	31.47999916106063	35.0	29.0	40.0	7.0	41.0
42	31.32477964733086	35.0	28.0	40.0	7.0	41.0
43	31.23358868550435	35.0	28.0	40.0	7.0	41.0
44	31.14388159769012	35.0	27.0	40.0	7.0	41.0
45	31.094319506563828	35.0	27.0	40.0	7.0	41.0
46	31.019057906042285	35.0	27.0	40.0	7.0	41.0
47	30.958407832897258	35.0	26.0	40.0	7.0	41.0
48	30.895036450167876	35.0	26.0	40.0	7.0	41.0
49	30.79237823581537	35.0	26.0	40.0	7.0	41.0
50	30.691191660942653	35.0	26.0	40.0	7.0	41.0
51	30.04709945451463	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	7.0
11	6.0
12	9.0
13	10.0
14	22.0
15	30.0
16	47.0
17	112.0
18	188.0
19	401.0
20	646.0
21	1085.0
22	1808.0
23	3109.0
24	6212.0
25	13151.0
26	23492.0
27	29107.0
28	26434.0
29	20584.0
30	16273.0
31	14703.0
32	14447.0
33	18465.0
34	33201.0
35	46954.0
36	45561.0
37	55991.0
38	94571.0
39	105455.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.411641332445456	14.8778906267751	17.836025804376817	37.87444223640263
2	51.46770695148658	17.521598319324795	17.563370508834204	13.447324220354417
3	23.39871817055288	17.3940096233337	45.930357545473136	13.276914660640285
4	21.049513152996326	18.995509926575327	45.80818699958578	14.146789920842576
5	18.27961499674037	21.790751042993893	44.62668071890113	15.302953241364605
6	19.859093141495865	23.899634886594622	43.903794627642	12.33747734426751
7	58.10022179459619	4.394294513161736	33.32476916058872	4.180714531653358
8	57.971060087284656	3.963114632326082	33.41233345742645	4.6534918229628195
9	54.081876987019164	5.243196289091516	35.339621883034376	5.335304840854949
10	30.022144503811056	18.61204472245963	40.23518267030906	11.130628103420252
11	23.379317697600808	17.813129750712662	43.61523443985941	15.192318111827122
12	21.398546887097986	15.36132943925642	48.251423138297405	14.988700535348187
13	16.75134710941692	19.69724775452634	48.71458758264864	14.836817553408105
14	13.664399782574879	21.798616099596085	48.02386083394069	16.513123283888344
15	12.183322234864573	18.38972578917104	53.514369458412204	15.912582517552185
16	14.032309652521798	17.531385945318632	50.948787994777604	17.487516407381968
17	13.922723197197943	18.09312576575065	47.375255832813366	20.608895204238042
18	15.843020461381698	17.061405118578836	49.47452682945586	17.621047590583604
19	16.598590232298815	18.76847195932542	46.75181901281305	17.88111879556271
20	17.633107344040297	19.516176673640352	46.85144306310747	15.999272919211888
21	16.699437735842462	19.3532826124572	47.89504868470037	16.05223096699997
22	15.830611149853796	18.269477812675326	46.21472303640123	19.685188001069648
23	15.02400590054024	19.35607907702687	47.12846783454018	18.491447187892707
24	16.83384281422212	17.555155894160805	48.293894443949235	17.317106847667834
25	15.465148186405337	19.01753208506146	46.619686061896246	18.897633666636953
26	14.800288734966818	20.702576767321915	46.62021039900306	17.87692409870821
27	15.686942782587115	21.054406965993245	46.47951327534165	16.779136976077993
28	13.237764156664936	19.881464858053207	48.09150032071953	18.789270664562327
29	15.410966685368024	19.583990939454797	46.46046236046079	18.544580014716395
30	16.96248018442684	20.632665153080218	45.27633439424208	17.128520268250863
31	18.410699273443548	21.718567301289347	41.698083198316525	18.17265022695058
32	18.36123680636755	22.804644228534077	41.03060206134395	17.803516903754428
33	18.336592962347353	23.907499943196814	38.817899470594305	18.938007623861534
34	18.585478309047787	23.053005238127696	38.566217659324195	19.79529879350032
35	19.002326308963895	24.29306249573976	36.85285877329586	19.851752422000484
36	21.394352190243485	26.22541951338021	34.215617905063525	18.16461039131278
37	18.838383573567118	26.738046424807436	36.012870728181895	18.410699273443548
38	17.55323332476916	28.39180565969473	34.05656898266366	19.99839203287244
39	20.34078416362114	25.952065101695183	35.260796537976866	18.446354196706814
40	21.794421402741584	24.220354416928398	35.903808609964855	18.081415570365166
41	18.15831834603103	25.77466438055688	34.46380413562154	21.603213137790547
42	18.77860914339047	25.83478836880474	34.348974309229554	21.037628178575236
43	19.43036016715867	26.198503541897157	34.078765920185404	20.292370370758768
44	18.53828796943464	25.662281460663355	34.161436404026205	21.63799416587579
45	19.248939528201472	24.9752250717031	33.48591543141583	22.289919968679598
46	20.339385931336306	24.900769202535695	35.11957507720864	19.64026978891936
47	17.4807000249934	24.085425001441926	38.52182378428072	19.912051189283947
48	17.15403800744908	23.42301245650187	38.103577552079784	21.31937198396927
49	18.65906028303717	22.53216371202707	38.66811383708147	20.14066216785429
50	18.295170330909148	22.4302675342698	37.62678034295142	21.647781791869626
51	16.93748678233543	22.529192468421797	35.80488367581285	24.728437073429916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	714.0
1	831.0
2	948.0
3	23100.5
4	45253.0
5	30689.0
6	16125.0
7	15677.5
8	15230.0
9	15283.0
10	15336.0
11	14786.5
12	14237.0
13	13255.0
14	12273.0
15	11202.5
16	10132.0
17	9133.0
18	8134.0
19	7293.5
20	6453.0
21	5865.5
22	5278.0
23	4950.0
24	4622.0
25	4496.5
26	4546.0
27	4721.0
28	5010.0
29	5299.0
30	5404.0
31	5509.0
32	6284.0
33	7059.0
34	8419.0
35	9779.0
36	10294.0
37	10809.0
38	11950.5
39	13092.0
40	14867.0
41	16642.0
42	19085.5
43	21529.0
44	26254.0
45	30979.0
46	34712.0
47	38445.0
48	43546.0
49	48647.0
50	47944.0
51	47241.0
52	40723.0
53	34205.0
54	29704.5
55	25204.0
56	22792.5
57	20381.0
58	18766.0
59	17151.0
60	16479.5
61	15808.0
62	14149.5
63	12491.0
64	11559.5
65	10628.0
66	8440.0
67	6252.0
68	5163.5
69	4075.0
70	3363.0
71	2651.0
72	2175.0
73	1699.0
74	1541.0
75	1042.5
76	702.0
77	549.5
78	397.0
79	280.5
80	164.0
81	95.5
82	27.0
83	40.5
84	54.0
85	35.0
86	16.0
87	8.5
88	1.0
89	1.0
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	572151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.0967077527176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2200028082662	27.873878740340174
2	10.093891659221613	7.287125146210327
3	3.9076345268564356	4.23158224561097
4	1.9607130156318109	2.831011388488444
5	1.086630827649062	1.9611897710370922
6	0.682350131071297	1.4778355959785494
7	0.47728459061309875	1.2059881667565542
8	0.33630601366373575	0.9711631912561056
9	0.26942098419828553	0.8752689476149553
>10	3.4763164756323883	32.14261635399031
>50	0.43961595743114346	9.834668823319383
>100	0.04489904840203951	3.120664018872522
>500	0.0024669806814307423	0.595031302765272
>1k	0.0019735845451445935	1.3597916781241697
>5k	0.0	0.0
>10k+	4.933961362861484E-4	4.232184629635186
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23763	4.153274223063492	No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2900	0.5068592032522883	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2146	0.37507581040669336	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	1550	0.27090750518656787	TruSeq Adapter, Index 21 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1039	0.1815954179928026	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	848	0.14821262219239326	No Hit
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	770	0.13457985741526277	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	626	0.10941167628825259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.495580712084747E-4	0.0	0.0	0.29380355885072296	0.0
2	3.495580712084747E-4	0.0	0.0	1.4849226864936005	0.0
3	3.495580712084747E-4	0.0	0.0	2.053828447385393	0.0
4	3.495580712084747E-4	0.0	0.0	2.6043824095387405	0.0
5	3.495580712084747E-4	0.0	0.0	3.994225300663636	0.0
6	3.495580712084747E-4	0.0	0.0	4.700682162575963	0.0
7	3.495580712084747E-4	0.0	0.0	5.521444513773462	0.0
8	3.495580712084747E-4	0.0	0.0	6.690191924858997	0.0
9	3.495580712084747E-4	0.0	0.0	7.10529213441906	0.0
10	3.495580712084747E-4	0.0	0.0	8.045778125005462	0.0
11	3.495580712084747E-4	0.0	0.0	9.506756083621282	0.0
12	3.495580712084747E-4	0.0	0.0	10.661521171858478	0.0
13	3.495580712084747E-4	0.0	0.0	11.111751967574993	0.0
14	5.24337106812712E-4	0.0	0.0	11.29719252435109	0.0
15	5.24337106812712E-4	0.0	0.0	11.654790431197359	0.0
16	5.24337106812712E-4	0.0	0.0	12.445840346342138	0.0
17	5.24337106812712E-4	0.0	0.0	13.338262102137373	0.0
18	5.24337106812712E-4	0.0	0.0	14.286263591254755	0.0
19	5.24337106812712E-4	0.0	0.0	14.839264459906563	0.0
20	5.24337106812712E-4	0.0	0.0	15.39313922373639	0.0
21	5.24337106812712E-4	0.0	0.0	16.033354831154714	0.0
22	5.24337106812712E-4	0.0	0.0	16.694718701881147	0.0
23	5.24337106812712E-4	0.0	0.0	17.369016221242294	0.0
24	5.24337106812712E-4	0.0	0.0	17.913452917149495	0.0
25	5.24337106812712E-4	0.0	0.0	18.38308418581808	0.0
26	5.24337106812712E-4	0.0	0.0	18.81583707797417	0.0
27	5.24337106812712E-4	0.0	0.0	19.24474483134697	0.0
28	5.24337106812712E-4	0.0	0.0	19.685537559140855	0.0
29	5.24337106812712E-4	0.0	0.0	20.15272192131098	0.0
30	5.24337106812712E-4	0.0	0.0	20.649443940498227	0.0
31	5.24337106812712E-4	0.0	0.0	21.12938717226746	0.0
32	6.991161424169494E-4	0.0	0.0	21.54606039314796	0.0
33	6.991161424169494E-4	0.0	0.0	21.98528010962141	0.0
34	6.991161424169494E-4	0.0	0.0	22.412789630709376	0.0
35	6.991161424169494E-4	0.0	0.0	22.875779296025	0.0
36	8.738951780211867E-4	0.0	0.0	23.31132865275076	0.0
37	8.738951780211867E-4	0.0	0.0	23.73324524469939	0.0
38	8.738951780211867E-4	0.0	0.0	24.165473799748668	0.0
39	8.738951780211867E-4	0.0	0.0	24.610286445361453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATG	30	2.1635824E-6	45.000004	9
GCGATAT	30	2.1635824E-6	45.000004	9
TTCGACC	30	2.1635824E-6	45.000004	22
TGTAACG	30	2.1635824E-6	45.000004	1
CGGGTAC	35	1.210501E-7	45.0	6
CACGGAT	20	7.0303126E-4	45.0	4
CCAGCGT	35	1.210501E-7	45.0	23
CGATTGC	20	7.0303126E-4	45.0	11
CGATTGA	25	3.888342E-5	45.0	10
TACGGAC	20	7.0303126E-4	45.0	9
CGCTAAG	20	7.0303126E-4	45.0	1
ACCAGTT	20	7.0303126E-4	45.0	12
CCGAGGT	20	7.0303126E-4	45.0	31
GTCCGTT	20	7.0303126E-4	45.0	9
TGTTACG	20	7.0303126E-4	45.0	1
ACGCTTT	20	7.0303126E-4	45.0	11
AATTACG	20	7.0303126E-4	45.0	19
CGTCGCT	20	7.0303126E-4	45.0	32
GACGCTT	20	7.0303126E-4	45.0	10
GTCCCGC	25	3.888342E-5	45.0	15
>>END_MODULE