SRR2934945.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934945.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754850
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28066	3.71808968669272	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	3596	0.47638603696098564	RNA PCR Primer, Index 41 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	3205	0.42458766642379275	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	1818	0.2408425515002981	RNA PCR Primer, Index 41 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1238	0.16400609392594556	Illumina PCR Primer Index 3 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTG	924	0.12240842551500299	RNA PCR Primer, Index 41 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCC	851	0.11273762999271378	No Hit
ACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	830	0.10995562032191826	TruSeq Adapter, Index 11 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCAAC	30	2.1645083E-6	45.000004	19
ACGATAC	35	1.2111923E-7	45.000004	45
ACGGCAT	60	0.0	45.000004	45
TATTCGG	30	2.1645083E-6	45.000004	1
AACGATA	35	1.2111923E-7	45.000004	44
GTATACG	30	2.1645083E-6	45.000004	1
CACACGG	65	0.0	45.000004	2
TGCGTAG	30	2.1645083E-6	45.000004	1
TCACGGC	50	2.1827873E-11	45.0	43
AACCGGA	20	7.0317474E-4	45.0	10
GATACGT	20	7.0317474E-4	45.0	9
GCCTTAC	25	3.889529E-5	45.0	35
CATGCGA	25	3.889529E-5	45.0	43
TCCGAAC	25	3.889529E-5	45.0	16
TAGCGAG	20	7.0317474E-4	45.0	1
CTCCCGA	20	7.0317474E-4	45.0	14
TCCCTCG	25	3.889529E-5	45.0	12
GAATCGG	40	6.8102963E-9	45.0	2
CCTACGG	20	7.0317474E-4	45.0	2
TAACGCG	40	6.8102963E-9	45.0	1